• Proceedings of the Korean Society of Life Science Conference
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• 2007.10a
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• pp.73.2-73.2
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• 2007

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• Proceedings of the PSK Conference
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• 2001.04a
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• pp.202.1-202.1
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• 2001

• Proceedings of the PSK Conference
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• 2001.10a
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• pp.211.1-211.1
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• 2001

• Toxicological Research
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• v.33 no.3
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• pp.225-231
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• 2017

National reference standards (NRSs) for biologics are established through potency estimation by a multi-center joint study of standard materials used in the approval process for national lot release and quality control of vaccines, blood products, and other biologics. In this study, a stability evaluation was conducted to determine whether the potency of NRSs for six blood products was being maintained at a consistent level in Korea. The present study conducted real-time stability tests via in-vivo/in-vitro bioassay on NRSs for blood coagulation factor VIII concentrate (2nd standard), antithrombin concentrate, prekallikrein activator, anti-hepatitis B immunoglobulin, blood coagulation factor IX concentrate, and anti-tetanus human immunoglobulin, as well as a trend analysis using cumulative annual results. The real-time stability test results showed that the mean potency of six NRSs was all within the control limit. In the trend analysis, the potency of NRS for blood coagulation factor VIII concentrate (2nd standard) showed a decreasing trend, while the potency of all other products had been stably maintained. The present study confirmed that the mean potency of NRSs for six blood products had been stably maintained in Korea. The findings of the present study establish a foundation that can ensure the quality of NRSs for biologics in Korea, and it is expected to make a major contribution to the supply of high-quality biologics.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.47 no.5
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• pp.489-494
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• 2014

This study determined the effect of packaging and electron beam irradiation on the quality of dried Undaria pinnatifida. Samples were air or vacuum packaged and irradiated at 7 kGy. The dried Undaria pinnatifida had total viable cells and coliform counts of 5.51 and 4.40 log CFU/g in total, respectively, and counts of 5.56 and 4.19 log CFU/g in surface. These counts were reduced by 2-4 log cycles after irradiation. Irradiation increased the lightness and yellowness of the dried Undaria pinnatifida, but not the redness. In the sensory evaluation, there were no significant differences among samples. Therefore, electron beam irradiation improves the microbial safety and quality of dried Undaria pinnatifida.

• Food Science and Preservation
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• v.16 no.2
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• pp.253-258
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• 2009

This study investigated the effects of mycelium and culture supernatant of Cordyceps ochraceostromat(Co) on air way hyper-responsiveness, pulmonary immune cell infiltration, and Th2 cytokine expression in animal models of atopy and asthma. After ConA(+/-) activation of mouse primary spleen cells, decreased IL-4 and IL-13 cytokine production were seen in the presence of Co mycelium extracts and culture supernatant. The asthma model involved mice sensitized to ovalbumin by i.p. injection treatment; Co mycelium extract was also injected. The atopy model was the dinitrofenylbenzene-treated mouse ear. Ear thicken ing induced by DNFB was decreased by Co mycelium extract, and the extract also inhibited lung cell infiltration in ovalbumin-induced asthmatic mice. The results thus indicated that the Co mycelial extract reduced the undesirable immune responses seen in asthma and atopy.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.47 no.6
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• pp.751-756
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• 2014

The anti-inflammatory effects of the ethanol extract of Undaria pinnatifida root (UPREE) were investigated using the lipopolysaccharide (LPS)-induced inflammatory response in RAW 264.7 cells by measuring the production of nitric oxide (NO), interleukin (IL)-6, tumor necrosis factor (TNF)-${\alpha}$, and IL-$1{\beta}$, and cell proliferation. We found that NO levels were reduced by 34% at $100{\mu}g/mL$. Moreover, the production of IL-6 and TNF-${\alpha}$ was suppressed by the UPREE treatment. In particular, the IL-6 production was inhibited by more than 30% at $100{\mu}g/mL$ UPREE. The proliferation of RAW 264.7 cells was measured by MTT assay, and we found no cytotoxicity in those cells treated with UPREE compared to the control. Our results suggest that UPREE shows promise as a therapeutic anti-inflammatory treatment.

• Journal of the Korean Home Economics Association
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• v.44 no.8
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• pp.79-87
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• 2006

The purpose of this study was to estimate the minimum monthly food cost for the low income population. The food consumption data of 9,311 individuals from the 2001 Korean National Health and Nutrition Survey was used. The monthly food cost was calculated using the Consumer Food Price Database for the year 2001 provided by the Public Health Nutrition Laboratory, Seoul National University. The low income population (n = 1,310) was characterized as older age, lower income, smaller family size, lower education level, and lower energy intake as compared with the total population (n = 8,001). The estimated food cost showed that men in the low income population needed 15% more money for purchasing food to maintain the energy intake level at the average energy intake level of men in the total population. It was also estimated that women in the low income population needed 9% more money for purchasing food to maintain the energy intake level at the average energy intake level of women in the total population. There were differences in monthly food costs depending on the sex and age, and family size. The results of this study could be used as basic information to establish minimum food cost for the low income population in Korea.

• Journal of Microbiology and Biotechnology
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• v.31 no.12
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• pp.1684-1691
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• 2021

In this study, the physicochemical changes related to fishy smell were determined by storing high hydrostatic pressure (HHP)-treated mackerel (Scomber japonicus) meat in a refrigerator for 20 days. The inhibition of crude urease activity from Vibrio parahaemolyticus using HHP treatment was also investigated. The mackerel meat storage experiment demonstrated that production of trimethylamine (TMA) and volatile basic nitrogen (VBN), the main components of fishy smell, was significantly reduced on the 20th day of storage after the HHP treatment compared to the untreated mackerels. The results demonstrated that the increased ammonia nitrogen rates in the 2000, 3000, and 4000 bar, HHP-treated groups decreased by 23.8%, 23.8%, and 31.0%, respectively, compared to the untreated groups. The enzyme activity of crude urease was significantly reduced in the HHP-treated group compared to that in the untreated group. Measurement of the volatile organic compounds (VOCs) in mackerel meat during storage indicated that the content of ethanol, 2-butanone, 3-methylbutanal, and trans-2-pentenal, which are known to cause off-flavor due to spoilage, were significantly reduced by HHP treatment. Collectively, our results suggested that HHP treatment would be useful for inhibiting the activity of urease, thereby reducing the fishy smells from fish and shellfish.

• Journal of Microbiology and Biotechnology
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• v.32 no.6
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• pp.808-815
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• 2022

In this study, gamma-irradiated mackerel (Scomber japonicus) meat was stored in a refrigerator for 20 days to examine the physicochemical changes related to fishy smell. The effect of gamma irradiation on the inhibition of the activity of crude urease extracted from Vibrio parahaemolyticus was also evaluated. Increased levels of trimethylamine (TMA) and volatile basic nitrogen (VBN) content, which are the main components causing fishy smell, were significantly reduced by day 20 of storage after gamma irradiation, indicating that freshness was maintained during storage. The ammonia nitrogen contents of 3, 7, 10, and 20 kGy gamma-irradiated groups were significantly decreased by 6.5, 15.2, 17.4, and 23.9%, respectively, compared to non-irradiated groups on day 20 of storage. In addition, urease activity decreased in a gamma irradiation intensity-dependent manner. Volatile organic compounds (VOCs) were measured during the storage of gamma-irradiated mackerel meat. The contents of ethanol, 2-butanone, 3-methylbutanal, and trans-2-pentenal, which are known to cause off-flavors due to spoilage of fish, were significantly reduced by day 20 of storage. Therefore, gamma irradiation can be considered useful for inhibiting urease activity and reducing fishy smell during fish storage.