• Korean Journal of Microbiology
• /
• v.45 no.2
• /
• pp.228-231
• /
• 2009

We have purified Phytophthora capsici alpha and beta tubulin from Escherchia coli BL21(DE3). The recombinant alpha and beta tubulins were assembled into microtubule in vitro with specific conditions. The metagenome library was isolated from soil in the Mt. Yeo-Ki, Suwon, Korea and manufactured with the method mentioned in experiment contents for in vitro screening of microtubule assembly screening. FRET effect was used for microtubule assembly inhibitor screening with metagenome library. We got 2 metagenome clones from in vitro screening, and these 2 hit clones showed P. capsici growth inhibition activity on the growing pepper plants. These results suggest that new development of potent inhibitor for pepper blight disease and new approach to prevention of pepper blight disease.

• Korean Journal of Mineralogy and Petrology
• /
• v.33 no.4
• /
• pp.463-475
• /
• 2020

Presence of microplastics in soil and groundwater has recently been reported and environmental concerns are raised as to the plastic pollution. In the subsurface environment, clay minerals and metal oxide minerals are commonly found as finely dispersed states. Because the minerals have high sorption capacities for diverse pollutants, interactions with mineral surface play an important role in the transport of microplastics in groundwater. Accordingly, environmental mineralogy investigating the interactions between microplastics and mineral surfaces is the essential research area to understand the fate and transport of microplastics in the subsurface environment. The microplastic-mineral surface research requires molecular- to nano-scale analyses to be able to probe the relatively weak interactions between them. The current report introduces a nano-scale analysis tool called quartz crystal microbalance (QCM) that can measure the sorbed/desorbed mass of nanoplastics on mineral surfaces at the level of a few nanograms (~10-9 g). This report briefly reviews the main principles in the QCM measurement and discusses applications of QCM to the environmental mineralogy research.

• Journal of Korean Society of Environmental Engineers
• /
• v.34 no.4
• /
• pp.288-294
• /
• 2012

The current growth of nano-industries has resulted in released nanoparticles entering into water and soil ecosystems via various direct or indirect routes. Physicochemical properties of nanoparticles differ from bulk materials, and nanomaterials influence the fates of nanoparticles and the interactions of living or non-living things in the environment. Microcosm analysis is a research methodology for revealing natural phenomena by mimicking part of an ecosystem under controlled conditions. Microcosm study allows for the integrated analysis of toxic effects and fates of nanoparticles in the ecosystem. Ecotoxicity studies of nanomaterials are steadily increasing, and microcosm studies of nanomaterials are currently beginning to surface. In this study, microcosm studies of nanomaterials in water and soil ecosystems were extensively investigated based on SCI(E) papers. We found that the microcosm studies have been reported in 12 instances, and mesocosm studies have been reported in only once until now. Advanced research was mostly evaluated at the microorganism level. But integrated analysis of nanotoxicity is required to research the interactions based of various species. Thus, our studies analysed the trend of microcosm studies on nanomaterials in water and soil ecosystems and suggested future directions of microcosm research of nanomaterials.

• Proceedings of the Korean Geotechical Society Conference
• /
• 2009.09a
• /
• pp.1472-1477
• /
• 2009

The landfill ground that consists of sandy soil or contains boulder or has waste ground such as waste landfill can be often applied by dynamic compaction method considering quick construction and economic advantages. In this study, the improvement efficiency of the dynamic compaction method that is used on the waste disposal ground of Tague Freight Terminal constrution site is analyzed. The results show that the N values are increased from 6.5/30 to 22.5/30, which is 3.5 times increase compared with the N value before dynamic compaction. The amount of settlement is in the range of 0.706~1.729m. the $\alpha$ vlues suggested by Leonards et al.(1980) was about 0.25~0.48, which are quite similar to to not only 0.3~0.5 of the findings of waste layer of the Society of Soil Engineering of Japan (1987) but also 0.35~0.4 of that of mine waste of Lukas (1986).

• Journal of the Korean Geosynthetics Society
• /
• v.5 no.4
• /
• pp.27-33
• /
• 2006

Frictional properties between geostrip and sand paper interface were estimated considering soil particle size also the friction coefficients and angles were determined with normal stress. Three kinds of geostrips of design strength 50, 70, 100 KN/m were used and 5 sandpapers of P100, P220, P320, P400, P600 were used also. Shear strength between geostrip and sand paper interface with design strength showed big difference and this is due to the uniform surface pattern of each geostrip when contact to sandpaper without regard to design strength. Shear strength of geostrip was increased with design strength and geostrips/P100 sandpaper interface showed the biggest value. Finally, all of geostrips showed the decrease phenomena of post-peak strength and this is due to the abrasion of geostrip surface by shear test.

• Resources Recycling
• /
• v.16 no.4
• /
• pp.40-46
• /
• 2007

This research constructed property analysis for an high additional value construction materials. We try to utilize by the addition material of soil stabilizer. It was described to the material properties analysis of a sludge. A sample used 4 kinds of filtration cake which occurs by 4 companies and 3 kinds of sludge before filtration. ph of a sludge, a particle analysis and a scanning electron microscope observation were performed. We analyzed on the chemical ingredient by XRF, the phase by XRD, observed and analyzed the thermo-property of the sludge and the amount of the volatile constituent by TG-DTA.

• Mycobiology
• /
• v.48 no.5
• /
• pp.383-391
• /
• 2020

Use of nanoparticles (NPs) in several commercial products has led to emergence of novel contaminants of air, soil and water bodies. The NPs may exhibit greater ecotoxicity due to nano-scale dependent properties over their bulk counterparts. The present investigation explores the effect of in vitro supplementation of TiO₂, silica and silver NPs on radial growth and ultrastructural changes in the hyphae and spores of two mushroom genera, Ganoderma lucidum and Volvariella volvaceae. A concentration dependent decrease in radial growth on NP amended potato dextrose agar medium was recorded. However, in comparison to control, there was decrease in radial diameter on supplementation with TiO₂ NPs while an increase was recorded for silica and silver NPs amendments as compared to their bulk salts at same concentrations after 48 h of incubation. Optical microscopy studies showed decrease in the number of spores while increase in spore diameter and thinning of hyphal diameter on NPs supplementation. Scanning electron microscopy analysis of fungal growth showed presence of deflated and oblong spores in two fruiting strains of Ganoderma while Volvariella exhibited decreased sporulation. Further, hyphal thinning and branching was recorded in response to NP amendments in both the test mushrooms. Enhancement of protein content was observed on NP compared to bulk supplementation for all cultures, concentrations and hours of incubation except for TiO₂ NPs. Likewise, bulk and NP supplementations (at 100 mg L^-1) resulted in enhanced laccase activity with occurrence of laccase specific protein bands on SDS-PAGE analysis.

• Korean Journal of Microbiology
• /
• v.52 no.2
• /
• pp.212-219
• /
• 2016

Forty-nine pigments were extracted from the collections of 106 pigment producing bacteria from the plant rhizosphere soil. Antibacterial activity test was performed in the subjects of the extracted pigments with plant pathogenic bacteria including Xanthomonas axonopodis and Xanthomonas campestris, and with plant pathogenic fungi including Botrytis cinerea, Colletotrichum acutatum, and Fusarium oxysporum. The yellow pigment by Chryseobacterium sp. RBR9 and the red pigment by of Methylobacterium sp. RI13 showed the antibacterial activities against Xanthomonas axonopodis and Xanthomonas campestris. The violet pigment by Collimonas sp. DEC-B5 showed the antibacterial activity as well as the antifungal activities against Botrytis cinerea and Fusarium oxysporum. Especially, the violet pigment inhibited the growth of Botrytis cinerea more than 65% at MIC $20{\mu}M$. Upon the HPLC analysis result for the isolation of pigment with antifungal activity, violacein (91.6%) and deoxyviolacein (8.4%) were isolated for the pigment by Collimonas sp. DEC-B5. The production amount of the pigment was increased more than 10 times higher when D-mannitol 1.5% and yeast extract 0.2% were added as the nitrogen source to SCB medium. This study suggests that produced violacein by Collimonas sp. DEC-B5 will be effective to control strawberry gray-mold rot fungi by its preventive activity.

• Korean Journal of Environmental Agriculture
• /
• v.22 no.3
• /
• pp.177-184
• /
• 2003

Although clay can sorb significant amounts of inorganic contaminants from soils and wastewater, the hydration of exchangeable cations in clay minerals makes it hydrophilic at the clay mineral surfaces and interlayers. Thus, natural clays are often ineffective in complexing and stabilizing toxic organic contaminants in soils and groundwater environment. But, substituting these hydrated cations with cationic surfactant such as QAC(Quaternary ammonium Compound) can change the natural clay from hydrophilic to hydropobic. Furthermore functionalized organoclay can act as a powerful dual function sorbent for both toxic metals and organic compounds. It also can be used as landfill clay liners, slurry walls, nano-composite materials, petroleum tank farms, waste treatment, and filter systems. To use this modified clay minerals effectively, it is required to understand the fundamental chemistry of organoclay, synthetic procedures, its engineering application, bioavailability of sorbed ion-clay complex, and potential risk of organoclay. In this review, we investigate the use, application and historical background of the organoclay in remediation technology. The state-of-the-art of organoclay research is also discussed. Finally, we suggest some future implications of organoclay in environmental research.

• Journal of the Korea Fashion and Costume Design Association
• /
• v.11 no.2
• /
• pp.169-181
• /
• 2009

The purpose of this study was to provide basic information for developing a smart taekwondo uniform with enhanced functionality for children by investigating the wearing condition and satisfaction of taekwondo uniform among taekwondo training children. The data were collected from 162 children living in Seoul and Daegu. For data analysis were performed descriptive statistics, ${\chi}2$ -test and t-test using SPSS Ver. 12. The results of the analysis are as follows. The results revealed that respondents wearing the general training uniform during their training period were the most. The dissatisfaction for the general training uniform was a high price, lack of symbolic, lack of functionality in textiles, lack of variety in design and unfittness of size in order. The developed uniform can adjust the length using a zipper type fastener, and can be used in all seasons as the lining is detachable. It also uses nano-silver material, and soil release finish. It enhances the functionality of movement by treating with raglan sleeve and sleeve hem velcro tape, and designs the upper garment in the draping. Safety is improved through attaching GPS, and elastic material is mixed in the neckline for putting on and off easily. Piece of cloth is added inside the closing of the upper garment. Rings are made for fixing the taekwondo uniform belt. Pocket is designed in the lower garment and colors are multiplied.