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Pepper Blight Disease Inhibition Metagenome Clone Screening Using Soil Metagenome Library  

Park, Hae-Chul (Department of chemistry, Hanyang university)
Sung, So-Ra (Department of chemistry, Hanyang university)
Kim, Dong-Gwan (Research Center for Drugs, Kunkuk University)
Koo, Bon-Sung (Fermentation & Food Processing Division, Department of Korean Food Research for Globalization, National Academy of Agricultural Science, Rural Development Administration)
Jeong, Byeong-Moon (Department of Chemistry, Division of Nano Sciences, Ewha Womans University)
Kim, Jin-Heung (Department of Chemistry, Division of Nano Sciences, Ewha Womans University)
Yoon, Moon-Young (Department of chemistry, Hanyang university)
Publication Information
Korean Journal of Microbiology / v.45, no.2, 2009 , pp. 228-231 More about this Journal
Abstract
We have purified Phytophthora capsici alpha and beta tubulin from Escherchia coli BL21(DE3). The recombinant alpha and beta tubulins were assembled into microtubule in vitro with specific conditions. The metagenome library was isolated from soil in the Mt. Yeo-Ki, Suwon, Korea and manufactured with the method mentioned in experiment contents for in vitro screening of microtubule assembly screening. FRET effect was used for microtubule assembly inhibitor screening with metagenome library. We got 2 metagenome clones from in vitro screening, and these 2 hit clones showed P. capsici growth inhibition activity on the growing pepper plants. These results suggest that new development of potent inhibitor for pepper blight disease and new approach to prevention of pepper blight disease.
Keywords
FRET; metagenome; pepper blight disease; Phytophthora capsici;
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