• 생명과학회지
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• 제8권6호
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• pp.654-659
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• 1998

NR2B는 연접후 치밀질의 주요 tyrosine 인산화 단백질이다. 본 연구에서는 mass spectrometry 방법을 적용하여 NR2B의 tyrosine 인산화 위치를 동정하였다. NR2B를 N-octyl glucoside (NOG)에 용해되지 않는 PSD 분획으로부터 SDS-PAGE와 electroelution방법으로 분리하였다. 분리한 단백질을 NR2B와 phos-photyrosine에 특이한 항체로 조사한 결과 이들은 phosphotyrosine을 유지하고 있는 NR2B임이 확인되었다. 이 단백질을 trypsin 혹은 endolys-C 처리하고, matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry 방법으로 조사한 결과 Tyr-1304이 인산화됨을 확인하였다.

• 한국방사선학회논문지
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• 제5권4호
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• pp.217-221
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• 2011

$^{237}Np$ is very important material in the fission products of nuclear reactors. Resonance integral(RI) tests of this material is necessary to check between the experiments and the evaluated data. Such feedback to the evaluated data is very important to correct data and improve of codes. The RI for the $^{237}Np(n,{\gamma})^{238}Np$ reaction were measured by using the 46-MeV electron linear accelerator (linac) at the Research Reactor Institute, Kyoto University (KURRI). The measurement was performed in the energy region from 0.005 eV and 10 keV. RI obtained as 804.7 barns, compared with those of the evaluated data in JENDL-4.0 and Mughabghab.

• 전기학회논문지P
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• 제54권1호
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• pp.29-33
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• 2005

Energy distribution function for electrons in $SF_6$-Ar mixtures gas by MCS-BEq algorithm has been analysed over the E/N range $30{\sim}300$[Td] by a two term Boltana equation and by a Monte Carlo Simulation using a set of electron cross sections determined by other, authors, experimentally the electron swarm parameters for 0.2[%} and 0.5[%] $SF_6$-Ar mixtures were measured by time-of-flight(TOF) method. The result show that the deduced electron drift velocities, the electron ionization or attachment coefficients, longitudinal and transverse diffusion coefficients and mean energy agree reasonably well with theoretical for a rang of E/N values. The results obtained from Booltemann equation method and Monte Carlo simulation have been compared with present and previously obtained data and respective set of electron collision cross sections of the molecules.

• 한국전기전자재료학회:학술대회논문집
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• 한국전기전자재료학회 1997년도 춘계학술대회 논문집
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• pp.154-159
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• 1997

This paper describes the electron transport characteristic in SiH$_4$ gas calculated for range of E/N values from 0.5~300(Td) by the Monte Calro simulation and Boltzmann equation method using a set of electron collision cross sections determined by the authors and the values of electron swarm parameters are obtained for TOF method. The results gained that the value of an electron swarm parameter such as the electron drift velocity, the electron ionization coefficients longitudinal and transverse diffusion coefficients, characteristics energy agree with thee experimental and theoretical for a range of E/N. The electron energy distributions function were analysed in monosilane at EN : 30, 50(Td) for a case of equilibrium region in the mean electron energy. The validity of the results obtained has been confirmed by a TOF method.

• 한국전기전자재료학회:학술대회논문집
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• 한국전기전자재료학회 2005년도 하계학술대회 논문집 Vol.6
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• pp.578-580
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• 2005

Energy distribution function for electrons in SF6-Ar mixtures gas used by MCS-BEq algorithm has been analysed over the E/N range 30~300[Td] by a two term Boltzmann equation and by a Monte Carlo Simulation using a set of electron cross sections determined by other authors, experimentally the electron swarm parameters for 0.2[%] and 0.5[%] $SF_6$-Ar mixtures were measured by TOF method, The results show that the deduced electron drift velocities, the electron ionization or attachment coefficients, longitudinal and transverse diffusion coefficients and mean energy agree reasonably well with theoretical for a rang of E/N values. The results obtained from Boltzmann equation method and Monte Carlo simulation have been compared with present and previously obtained data and respective set of electron collision cross sections of the molecules.

• 전기학회논문지P
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• 제53권2호
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• pp.65-69
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• 2004

This paper calculates and gives the analysis of electron swarm transport coefficients as described electric conductive characteristics of pure Ar, pure $SiH_4$, Ar-$SiH_4$ mixture gases($SiH_4$-0.5%, 2.5%, 5%) over the range of E/N = 0.01~300[Td], P = 0.1, 1, 5.0 [Torr] by Monte Carlo the backward prolongation method of the Boltzmann equation using computer simulation without using expensive equipment. The results have been obtained by using the electron collision cross sections by TOF, PT, SST sampling, compared with the experimental data determined by the other author. It also proved the reliability of the electron collision cross sections and shows the practical values of computer simulation. Electron swann parameters in argon were drastically changed by adding a small amount of mono-silane. The electron drift velocity in these mixtures showed unusual behaviour against E/N. It had negative slope in the medium range of E/N, yet the slope was not smooth but contained a small hump. The longitudinal diffusion coefficient also showed a corresponding feature in its dependence on E/N. A two-tenn approximation of the Boltzmann equation analysis and Monte Carlo simulation have been used to study electron transport coefficients.

• Journal of Animal Science and Technology
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• 제48권6호
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• pp.847-860
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• 2006

본 시험은 n-6 및 n-3 계열 다중불포화지방산 함량이 풍부한 사료원을 Charolais 거세우에게 각각 사료 급여기간을 달리하여 급여하였을 때 근내 지방산 조성을 조사하기 위하여 수행하였다. 시험 수행은 영국 IGER (Institute of Grassland and Environmental Research) 연구소 육우 사양실험실에서 실시 하였으며, 공시축은 총 28두 Charolais 거세우로서 ad libitum으로 조사료 급여를 하였다. 그리고, 두 개의 지방원료원으로서 C18:2 n-6 함량이 풍부한 대두와 C18:3 n-3함량이 풍부한 아마종실을 60일 및 90일간 공급하였으며, 농후사료는 총 건물섭취의 73%으로 하였다. 도체중, conformation 및 fatness score는 60일 사료급여구와 비교하여 90일 사료급여구에서 높게 조사되었다(P<0.05). 공시축의 m. longissimus thoracis의 중성지방내 총지방산 함량은 지방원료원에 의한 영향은 없었지만, 반면에 인지질 내 총지방산 함량은 아마종실 급여구에 대략 15% 높게 나타났다(P<0.05). 근육 중성지방 내 C18:3 n-3와 cis-9, trans-11 CLA 함량은 아마종실급여구에 유의성있게 높게 조사되었으며(P<0.001), 반면에 C18:2 n-6 함량은 대두 급여구에서 높았다 (P<0.001). 근육 인지질 내 CLA와 C18:3 함량은 아마종실급여구에 높게 나타났으며, C18:2 n-6 함량은 대두 급여구에 높았다. 한편, 근육 중성지방 내 C14:0, C16:0, C16:1, CLA, C18:1 n-9, C18:2 n-6 및 C18:3 n-3 함량은 90일 사료급여구에서 높게 조사되었다. 근내 불포화지방산:포화지방산 비율은 사료급여기간에 영향을 받지 않았지만, C18:2 n-6:C18:3 n-3 비율과 n-6:n-3 불포화지방산 비율은 아마종실 급여구에서 높게 나타났다. 따라서 본 시험은 대두 혹은 아마종실 같은 사료급여와 급여기간에 의하여 쇠고기 근내 불포화지방산 함량을 변화 시킬 수 있음을 보여주고 있다. 그리고, 아마종실 급여는 쇠고기 내 지방산 함량이 조성이 인체 건강과 관련한 영양적 지표에 보다 바람직한 것으로 증명되었다.

• Journal of Microbiology and Biotechnology
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• 제15권5호
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• pp.1054-1059
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• 2005

An extracellular protease was identified from Bacillus subtilis KCCM 10257 by N-terminal sequencing and mass spectral analysis. The molecular mass of the extracellular protease was estimated to be 28 kDa by SDS-PAGE. Sequencing of the N-terminal of the protease revealed the sequence of A(G,S,R)QXVPYG(A)V(P,L)SQ. The N-terminal sequence exhibited close similarity to the sequence of other proteases from Bacillus sp. A mass list of the monoisotopic peaks in the MALDI-TOF spectrum was searched after peptide fragmentation of the protease. Six peptide sequences exhibiting monoisotopic masses of 1,276.61, 1,513.67, 1,652.81, 1,661.83, 1,252.61, and 1,033.46 were observed from the fragmented protease. These monisotopic masses corresponded to the lytic enzyme L27 from Bacillus subtilis 168, and the Mowse score was found to be 75. A doubly charged Top product (MS) at a m/z of 517.3 exhibiting a molecular mass of 1034.6 was further analyzed by de novo sequencing using a PE Sciex QSTAR Hybrid Quadropole-TOF (MS/MS) mass spectrometer. MS/MS spectra of the Top product (MS) at a m/z of 517.3 obtained from the fragmented peptide mixture of protease with Q-star contained the b-ion series of 114.2, 171.2, 286.2, 357.2, 504.2, 667.4, 830.1, and 887.1 and y-ion series of 147.5, 204.2, 367.2, 530.3, 677.4, 748.4, 863.4, and 920.5. The sequence of analyzed peptide ion was identified as LGDAFYYG from the b- and y-ion series by de novo sequencing and corresponded to the results from the MALDI-TOF spectrum. From these results the extracellular protease from Bacillus subtilis KCCM 10257 was successfully identified with the lytic enzyme L27 from Bacillus subtilis 168.

• Journal of Chest Surgery
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• 제33권4호
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• pp.290-300
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• 2000

Background: To evaluate the diagnostic accuracy of EBT(Electron Beam Tomography) in the diagnosis of conotruncal anomaly and to determine whether it can be used as a substitute for cardiac angiography. Material and Method: 20 patients(11M & 9F) with TOF(n=7, pulmonary atresia 2), DORV(n=7), complete TGV(n=4), & corrected TGV(n=2) were included. The age ranged from 7 days to 26 years(median 60 days). We analyzed the sequential chamber localization, the main surgical concenrn in each disease category (PA size, LVED volume and coronary artery pattern for TOF & pulmonary atresia, the LV mass, LVOT obstruction, coronary artery pattern for complete TGV, and type of VSD and TV-PV distance for DORV, etc) and other associated anomalies(e.g., VSD, arch anomalies, tracheal stenosis, etc). Those were compared with the results of echocardiography(n=19), angiography (n=9), and surgery(n=11). The interval between EBT and echocardiography/angiography was within 20/11 days, respectively except for an angiography in a patient with corrected TGV (48 days). Result: EBT correctly diagnosed the basic components of conotruncal anomalies in all subjects, compared to echocardiography, angiography or surgery. These included the presence, type and size of VSD(n=20), pulmonic/LV outflow tract stenosis(n=15/2), relation of great arteries and the pattern of the proximal epicardial coronary arteries(16 out of 20). EBT proved to be accurate in quantitation of the intrapericardial and hilar pulmonary arterial dimension and showed high correlation and no difference compared with echocardiography, angiography, or surgery(p>0.05) except for left pulmonary arterial & ascending arterial dimension by echocardiography. LVED volume in seven TOF(no difference: p>0.05 & high correlation: r=0.996 with echocardiography), and LV mass in 4 complete TGV were obtained. Additionally, EBT enabled the cdiagnosis of subjlottic tracheal stenosis and tracheal bronchus in 1 respectively. Some peripheral PA stenosis were not detected by echocardiography, while echocardiography appeared to be slightly more accurate than EBT in detecing ASD or PDA. Conclusion: EBT can be a non-invasive and accurate modality of for the evaluation of most anatomical alteration including peripheral PS or interruption in patients with conotruncal anomalies. Combined with echocardiography, EBT study provides sufficient information for the palliative or total repair of anomalies.

• Bulletin of the Korean Chemical Society
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• 제34권6호
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• pp.1708-1714
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• 2013

Polyhexamethylene guanidine (PHMG) polymers used as an active ingredient in an antibacterial humidifier disinfectant were reported to cause harm to the human health when inhaled, although physical contact with this material was known to present low toxicity to humans. It is therefore necessary to develop an optimal analysis method which enables detection and analysis of PHMG polymers. MALDI-TOF investigations of PHMG are performed with a variety of matrices, and it is found that CHCA and 2,5-DHB are excellent matrices which well reflects the polymer population even at high mass. For the provided PHMG sample, the number-average ($M_n$) and weight-average ($M_w$) molecular masses were determined to be 744.8 and 810.7, respectively, when the CHCA was used as a matrix. The rank of the matrices in terms of averaged molecular weight was CHCA ~2,5-DHB > 5-NSA > DHAP, THAP > ATT > IAA ~ super-DHB ~ HABA. In addition, PSD of the PHMG oligomer ions exhibited a few unique fragmenation characteristics. The formation of a- and c-type fragments was the major fragmentation pathway, and the 25-Da loss peaks generally accompanied a- and c-type fragments.