Journal of Animal Science and Technology

  • 제48권6호
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  • Pages.847-860
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  • 2006
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  • 2672-0191(pISSN)
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  • 2055-0391(eISSN)
한국축산학회 (Korean Society of Animal Sciences and Technology)
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사료급원과 급여기간이 Charolais 거세우 근내 지방산 조성에 미치는 영향

Effects of Diet and Time on Feed on Fatty Acid Composition in Muscle of Charolais Steers

  • 발행 : 2006.12.31
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초록

본 시험은 n-6 및 n-3 계열 다중불포화지방산 함량이 풍부한 사료원을 Charolais 거세우에게 각각 사료 급여기간을 달리하여 급여하였을 때 근내 지방산 조성을 조사하기 위하여 수행하였다. 시험 수행은 영국 IGER (Institute of Grassland and Environmental Research) 연구소 육우 사양실험실에서 실시 하였으며, 공시축은 총 28두 Charolais 거세우로서 ad libitum으로 조사료 급여를 하였다. 그리고, 두 개의 지방원료원으로서 C18:2 n-6 함량이 풍부한 대두와 C18:3 n-3함량이 풍부한 아마종실을 60일 및 90일간 공급하였으며, 농후사료는 총 건물섭취의 73%으로 하였다. 도체중, conformation 및 fatness score는 60일 사료급여구와 비교하여 90일 사료급여구에서 높게 조사되었다(P<0.05). 공시축의 m. longissimus thoracis의 중성지방내 총지방산 함량은 지방원료원에 의한 영향은 없었지만, 반면에 인지질 내 총지방산 함량은 아마종실 급여구에 대략 15% 높게 나타났다(P<0.05). 근육 중성지방 내 C18:3 n-3와 cis-9, trans-11 CLA 함량은 아마종실급여구에 유의성있게 높게 조사되었으며(P<0.001), 반면에 C18:2 n-6 함량은 대두 급여구에서 높았다 (P<0.001). 근육 인지질 내 CLA와 C18:3 함량은 아마종실급여구에 높게 나타났으며, C18:2 n-6 함량은 대두 급여구에 높았다. 한편, 근육 중성지방 내 C14:0, C16:0, C16:1, CLA, C18:1 n-9, C18:2 n-6 및 C18:3 n-3 함량은 90일 사료급여구에서 높게 조사되었다. 근내 불포화지방산:포화지방산 비율은 사료급여기간에 영향을 받지 않았지만, C18:2 n-6:C18:3 n-3 비율과 n-6:n-3 불포화지방산 비율은 아마종실 급여구에서 높게 나타났다. 따라서 본 시험은 대두 혹은 아마종실 같은 사료급여와 급여기간에 의하여 쇠고기 근내 불포화지방산 함량을 변화 시킬 수 있음을 보여주고 있다. 그리고, 아마종실 급여는 쇠고기 내 지방산 함량이 조성이 인체 건강과 관련한 영양적 지표에 보다 바람직한 것으로 증명되었다.

This study investigated the effects of feeding Charolais steers on diets rich in either n-6 or n-3 polyunsaturated fatty acids (PUFA) and time on feed (TOF) on muscle fatty acid composition and content. Twenty eight steers were fed on ad libitum forage and one of two concentrates varying in the source of fat; soya (high in C18:2 n-6) or whole linseed (high in C18:3 n-3) for either 60 or 90 days in IGER (Institute of Grassland and Environmental Research, UK). The concentrates were fed at approximately 0.73 of total DM intake. TOF influenced carcass weight, conformation and fatness scores, which were higher at 90 v. 60 days (P<0.05). Diet did not affect total fatty acid content of neutral lipid in m. longissimus thoracis but feeding linseed increased total phospholipid fatty acid by approx- imately 15%(P<0.05). Linseed increased the amount and proportion of C18:3 n-3 (P<0.001) and the proportion of CLA (cis-9, trans-11 conjugated linoleic acid), while soya increased the content (P<0.05) and proportion (P<0.001) of C18:2 n-6 in muscle neutral lipid. In muscle phospholipid, linseed significantly increased the amount of CLA, C18:3 n-3 and its longer chain derivatives as well as C14:0, C16:0, C18:0. C18:1 trans and C18:2 n-6. The amount and proportion of C18:2 n-6 and its longer chain C20 derivatives were higher on feeding soya. TOF (90 v. 60 day) increased the content of C14:0, C16:0, C16:1, CLA, C18:1 n-9, C18:2 n-6 and C18:3 n-3 in muscle neutral lipid. The P:S was not affected by diet or TOF. The ratio of C18:2 n-6 : C18:3 n-3 and sum of n-6 : n-3 fatty acids were higher in muscle from animals fed on linseed v. soya (P<0.001). The study indicates that the PUFA composition of beef muscle may be significantly modified by feeding contrasting dietary lipids, soya vs. linseed. Feeding linseed produced a better balance of muscle fatty acids, more in line with current nutritional recommendations with a lower C18:2 n-6:C18:3 n-3 ratio associated with higher muscle content of C18:3 n-3 and C20:5 n-3 and CLA and lower C20:4 n-6.

키워드

참고문헌

  1. Chalupa, W., Rickabaugh, B., Kronfeld, D. S. and Sklan, D. 1984. Rumen fermentation in vitro as influenced by long chain fatty acids. Journal of Dairy Science 67:1439-1444 https://doi.org/10.3168/jds.S0022-0302(84)81459-9
  2. Choi, N. J., Enser, M., Wood, J. D. and Scollan, N. D. 2000. Effect of breed on the deposition in beef muscle and adipose tissue of dietary n-3 polyunsaturated fatty acids. Animal Science 71: 509-519 https://doi.org/10.1017/S1357729800055417
  3. Clinquart, A., Istasse, L., Dufrasne, I., Mayombo, A., van Eenaeme, C. and Bienfait, J. M. 1991. Effects on animal performance and fat composition of two fat concentrates in diets for growing- fattening bulls. Animal Production 53:315-320 https://doi.org/10.1017/S0003356100020316
  4. Department of Health. 1994. Report on health and social subjects No. 46. Nutritional aspects of cardiovascular disease. HMSO, London
  5. Duckett, S. K., Wagner, D. G., Yates, L. D., Dolezal, H. G. and May, S. G. 1993. Effects of time on feed on beef nutrient composition. Journal of Animal Science 71:2079-2088
  6. Enser, M., Hallett, K. G., Hewett, B., Fursey, G. A. J., Wood, J. D. and Harrington, G. 1998. Fatty acid content and composition of UK beef and lamb muscle in relation to production system and implications for human nutrition. Meat Science 49:329-341 https://doi.org/10.1016/S0309-1740(97)00144-7
  7. Enser, M., Scollan, N. D., Choi, N. J., Kurt, E., Hallett, K. and Wood, J. D. 1999. Effect of dietary lipid on the content of conjugated linoleic acid (CLA) in beef muscle. Animal Science. 69:143-146 https://doi.org/10.1017/S1357729800051171
  8. French, P., Stanto, C., Lawless, F., O'Ricordan, E. G., Monahan, F. J., Caffrey, P. J. and Moloney, A. P. 2000. Fatty acid composition, including conjugated linoleic acid, of intramuscular fat from steers offered grazed, grass silage, or concentrate- based diets. Journal of Animal Science. 78:2849- 2855
  9. Gillis, M. H., Duckett, S. K., Sackmann, J. S. and Keisler, D. H. 2003. Effect of rumen- protected conjugated linoleic acid (CLA) or linoleic acid on leptin and CLA content of bovine adipose depots. Journal of Animal Science. 81 (suppl. 2):12 (Abstract)
  10. Hansen, H. S. 1994. New biological clinical roles for the n-6 and n-3 fatty acids. Nutrition Reviews 52:162-167 https://doi.org/10.1111/j.1753-4887.1994.tb01412.x
  11. Hecker, A. L., Cramer, D. A. and Hougham, D. F. 1975. Compositional and metabolic growth effects in the bovine muscle, subcutaneous and serum total fatty acids. Journal of Food Science 40:144-149 https://doi.org/10.1111/j.1365-2621.1975.tb03757.x
  12. Huerta-Leidenz, N. O., Cross, H. R., Savell, J. W., Lunt, D. K., Baker, J. F. and Smith, S. B. 1996. Fatty acid composition of subcutaneous adipose tissue from male calves at different stages of growth. Journal of Animal Science 74:1256- 1264
  13. Jenkins, T. C. 1993. Lipid metabolism in the rumen. Journal of Dairy Science 76:3851-3863 https://doi.org/10.3168/jds.S0022-0302(93)77727-9
  14. Jiang, J., Bjöerck, L., Fonden, R. and Emanuelson, M. 1996. Occurrence of conjgated cis-9, trans-11 octadecadienoic acid in bovine milk: effects of feed and dietary regimen. Journal of Dairy Science. 79:438-445 https://doi.org/10.3168/jds.S0022-0302(96)76383-X
  15. Kempster, A. J., Cook, G. L. and Grantley-Smith, M. 1986. National estimates of the body composition of British cattle, sheep and pigs with special reference to trends in fatness. A review. Meat Science 17:107-138 https://doi.org/10.1016/0309-1740(86)90059-8
  16. Lawes Agricultural Trust. 1990. GENSTAT V mark 2.2. Rothamsted Experimental Station, Harpenden, UK
  17. Lawless, F., Murphy, J. J., Harrington, D., Devery, R. and Stanton, C. 1998. Elevation of conjugated cis-9, trans-11-octadecadienoic acid in bovine milk because of dietary supplementation. Journal of Dairy Science. 81:3259-3267 https://doi.org/10.3168/jds.S0022-0302(98)75890-4
  18. Link, B. A., Bray, R. W., Cassens, R. G. and Kauffman, R. G. 1970. Fatty acid composition of bovine skeletal muscle lipids during growth. Journal of Animal Science 30:726-731 https://doi.org/10.2527/jas1970.305726x
  19. Maczulak, A. E., Dehority, B. A. and Palmquist, D. L. 1981. Effects of long-chain fatty acids on growth of rumen bacteria. Applied and Environmental Microbiology 42:856-862
  20. Madron, M. S., Peterson, D. G., Dwyer, D. A., Corl, B. A., Baumgard, L. H., Beermann, D. H. and Bauman, D. E. 2002. Effect of extruded full- fat soybeans on conjugated linoleic acid content of intramuscular, intermuscular, and subcutaneous fat in beef steers. Journal of Animal Science. 80:1135-1143
  21. Marmer, W. N., Maxwell, R. J. and Williams, J. E. 1984. Effects of dietary regimen and tissue site on bovine fatty acid profiles. Journal of Animal Science 59:109-121 https://doi.org/10.2527/jas1984.591109x
  22. Robelin, J. 1986. Growth of adipose tissues in cattle; portioning between depots, chemical composition and cellularity. Review of Livestock Production Science 14:349-364 https://doi.org/10.1016/0301-6226(86)90014-X
  23. Rule, D. C., MacNeil, M. D. and Short, R. E. 1997. Influence of sire growth potential, time on feed and growing-inishing strategy on cholesterol and fatty acids of the ground carcass and longissimus muscle of beef steers. Journal of Animal Science 75:1525-1533
  24. Sackmann, J. R., Duckett, S. K., Gillis, M. H., Realini, C. E., Parks, A. H. and Eggelston, R. B. 2003. Effects of forage and sunflower oil levels on ruminal biohydrogenation of fatty acids and conjugated linoleic acid formation in beef steers fed finishing diets. Journal of Animal Science. 81:3174-3181
  25. Scollan, N. D., Choi, N, J., Kurt, E., Fisher, A. V., Enser, M. and Wood, J. D. 2001a. Manipulating the fatty acid composition of muscle and adipose tissue in beef cattle. British Journal of Nutrition 85:115-124 https://doi.org/10.1079/BJN2000223
  26. Scollan, N. D., Choi, N, J., Maeng, W. J., Enser, M. and Wood, J. D. 2001b. Digestion of long chain fatty acids from different feed sources and their effect on the rumen function of steers. Journal of Agricultural Science, Cambridge, 136: 345-355
  27. Scollan, N. D., Enser, M., Gulati, S. K., Richardson, I. and Wood, J. D. 2003. Effects of including a ruminally protected lipid supplement in the diet on the fatty acid composition of beef muscle. British Journal of Nutrition. 90:709-716 https://doi.org/10.1079/BJN2003933
  28. Sinclair, A. J., Johnson, L., O'Dea, K. and Holman, R. T. 1994. Diets rich in lean beef increase arachidonic acid and long chain $\omega$-3 polyunsaturated fatty acids levels in plasma phospholipids. Lipids 29:337-343 https://doi.org/10.1007/BF02537187
  29. Solomon, R., Chase, L. E., Ben-hedalia, D. and Bauman, D. E. 2000. The effect of non-structural carbohydrate and addition of full fat extruded soybeans on the concentration of conjugated linoleic acid in the milk fat of dairy cows. Journal of Dairy Science. 83:1322-1329 https://doi.org/10.3168/jds.S0022-0302(00)74998-8
  30. Van Nevel, C. J. and Demeyer, D. I. 1996a. Influence of pH on lipolysis and biohydrogenation of soybean oil by rumen contents in vitro. Reproduction Nutrition Development 36:53-63 https://doi.org/10.1051/rnd:19960105
  31. Westerling, D. B. and Hedrick, H. B. 1979. Fatty acid composition of bovine lipids as influenced by diet, sex and anatomical location and the relationship to sensory characteristics. Journal of Animal Science 48:1343-1348 https://doi.org/10.2527/jas1979.4861343x