• 농촌의학ㆍ지역보건
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• 제15권2호
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• pp.89-96
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• 1990

The results of infection state of anisakid larvae and parasitic helminths in salmon and sea-trout which caught from Taep'o port. Kang-won do were as follows. 1) From twelve specimens of sea-trout. 122 individuals of anisakid larvae were found and mean infection number of anisakid larvae per individual sea-trout was 10.17. 2) From total extracted anisakid larvae from sea-trout. 37 larvae(30.3%) were found in the muscle. This percentage was lower than that of salmon. but higher than that of other sea-fishes. 3) The taxonomic list of parasitic helminths which found in salmon and sea-trout was as follows. Class Trematoda Family Hemiuridae l. Brachyphallus crenatus(Rudolphi. 1802) 2. Lecithaster salmonis Yamaguti. 1934 Class Cestoda Family Phllobothriidae 3. Pelichnibothrium speciosum Monticelli, 1889-larval form Family Tentacuariidae 4. Tentacularia sp. - larval form 4) Among those 4 species of helminths. B.crenatus. L.salmonis, P.speciosum were the first recording species in Korea.

• Parasites, Hosts and Diseases
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• 제46권2호
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• pp.59-63
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• 2008

In order to obtain greater insight into the relevant genomic expression patterns of Trichinella spiralis, 992 expressed sequence tags (ESTs) were collected from a cDNA library of T. spiralis muscle stage larvae and assembled into 60 clusters and 385 singletons. Of them, 445 (44.7%) ESTs were annotated to their homologous genes, and small fractions were matched to known genes of nematodes. The annotated ESTs were classified into 25 eukaryotic orthologous groups (KOG). Cytochrome C oxidase (34 clones) was found to be most frequent species.

• Parasites, Hosts and Diseases
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• 제50권4호
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• pp.339-343
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• 2012

The 5th outbreak of trichinosis occurred in a mountainous area of North Vietnam in 2012, involving 24 patients among 27 people who consumed raw pork together. Six of these patients visited several hospitals in Hanoi for treatment. Similar clinical symptoms appeared in these patients within 5-8 days after eating infected raw pork, which consisted of fever, muscle pain, difficult moving, edema, difficult swallowing, and difficult breathing. ELISA revealed all (6/6) positive reactions against Trichinella spiralis antigen and all cases showed positive biopsy results for Trichinella sp. larvae in the muscle. The larvae detected in the patients were identified as T. spiralis (Vietnamese strain) by the molecular analysis of the mitochondrial cytochrome c oxidase subunit III (cox3) gene.

• 한국어병학회지
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• 제16권1호
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• pp.13-21
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• 2003

The infection characteristics with scuticociliates at on-land rearing farms and hatcheries of flounder, Paralithys olivaceus was investigated during the year of 2001 by juvenile infection routes. When culture tanks for living food organisms such as chlorella, rotifer, and Artemia were searched, scuticocilates were detected both in live and dead rotifer, and at the dregs of culture tank bottoms at almost hatcheries. When rotifer infected with scuticocilates fed on fish larvae, lots of scuticocilate were inhabited at the bottom of fry rearing tanks. After feeding on scuticocilates-infected rotifer on fish larvae, first infection was detected at 10 days after bottom dwelling or 40 days old after hatching. By histopathological examination we confirmed the infection route of eyeball or brain contamination was that the ciliate worms digged through mouth and front part of the dosal fin cuticle, transferred into eyeball along the epithelium and muscle tissue, and reached finally into brain by the muscle and nerve tissue. The infection of internal organs was clarified into two routes. The first route was started from the infection at ventral and anal fin rays by the worms, and reached at the anus and rectum through the epithelium and muscle tissue. The second route was initiated from the infection at urinary organ and reached into the rectum epithelium cells, inner wall of intestine, abdominal cavity, pancreas, kidney, and pancreas. At seed production farms where fish larvae fed on scuticocilate-free rotifer, the worms were not detected not only at the food organisms culture tanks and juvenile rearing tanks but also larval flounder less than 7cm in total length.

• 한국해양학회지:바다
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• 제1권1호
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• pp.55-57
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• 1996

1995년 11, 12월에 동해안 주문진 어시장에서 구입한 살오징어(Todarodes pacificus) 248개체중 34개체로부터 116개체의 아니사키스 유충이 채집되어 13.7% 의 유충 감염율을 나타내었다. 형태 및 크기를 바탕으로 유충의 종류 및 근육, 내장, 망 등 어체 부위별 감염상황을 조사한 결과 유충의 길이는 13.5-29.5 mm 이었으며 Anisakis type I이 68개체 (58.6%:회수율), Contracaecum type A 5개 체 (4.3%), Anisakis type II 17개체 (14.6%), Contracaecum type D 8개체 (7.0%) 및 불명 18개체 (15.5%)로 분류되었다.

• Animal cells and systems
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• 제4권3호
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• pp.223-229
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• 2000

Limbs and supporting structures of an organism experience a full weight of its own when it lands from water, because neutral buoyancy in the aquatic habitat will be no longer available in the terrestrial world. Metamorphosis of anuran amphibians presents 8 good research model to examine how this transition from non-loading to weight-loading affects development of motor capacity at the time of their first emergence on land. Our video analysis of the transitional anurans, Rana catesbeiana, at Gosner stage 46 (the stage of complete transformation) demonstrated that the take-off speed increased 1.23-fold after the first six hours of weight-loading on the wet ground. It did not increase further during the following three days of loading, and was close to the level of mature frogs with different body mass. During development of larvae in deep water with no chance of landing through metamorphosis, both tension and power of a hindlimb anti-gravity muscle increased 5-fold between stages 37 and n. However, the muscle contractility increased more rapidly when the larvas could access the wet ground by their natural landing behavior after stages 41-42. Muscle power, one of major factors affecting locomotory speed, was 1.29-fold greater in the loaded than in the non-loaded larvae at the transitional stage. Thus, weight-loading had a potentially significant effect on the elevation of motor capacity, with a similar extent of increment in locomotory speed and muscle power during the last stages of metamorphosis. Such a motor adjustment of the froglets in a relatively short transitional period would be important for effective ecological interactions and survival in their inexperienced terrestrial life.

• Parasites, Hosts and Diseases
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• 제30권4호
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• pp.263-268
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• 1992

인체 소차관의 급성 질환 띤 만성으로 호산구성 육아종증을 일으키는 것으로 알려져 있는 Anisakis류에 있어서 중간 숙주가 되는 우리나라 남해안산 허산 어류 중 부산 각 지역별 광안리, 자갈치, 송도와 다대포에서 붕장어를 구입하여 유충류를 채집한 후 헙대학적인 조사를 시도하였던 바 Anisakis type과 Contracaecum type의 두가지 유충을 검출하였다. 붕장어 382마리 중 259마리가 양성으로 67.8%의 감염률이었으며 붕장어 마리당 감염를은 전체 충체 1,768마리로써 4.6마리였다. 넉장어의 크기에 따른 선충류의 감염률은 꽐수록 증가되었다. 통장어의 각 장기별 선충류의 감염률은 망 1,430(80.8%), 소장 166(9.38%), 위장 107(6.0%), 퍼부 32(1.8%), 근육 18(1.0%), 간장 13(0.7%) 그리고 두부 2(0.1%)의 순위이었다. 이상의 결과에 의하면 붕장어회의 생식으로 Anisakis류의 인체 감염의 가능성이 충분하다고 판단된다.

• Parasites, Hosts and Diseases
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• 제51권6호
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• pp.629-632
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• 2013

Members of the genus Trichinella are small nematodes that can infect a wide range of animal hosts. However, their infectivity varies depending on the parasite and host species combination. In this study, we examined the susceptibility of 4 species of laboratory rodents, i.e., mice, rats, hamsters, and gerbils to Trichinella papuae, an emerging non-encapsulated Trichinella species. Trichinella spiralis and Trichinella pseudospiralis were also included in this study for comparison. Fifteen animals of each rodent species were infected orally with 100 muscle larvae of each Trichinella species. Intestinal worm burden was determined at day 6 and 10 post-inoculation (PI). The numbers of muscle larvae were examined at day 45 PI. The reproductive capacity index (RCI) of the 3 Trichinella species in different rodent hosts was determined. By day 6 PI, 33.2-69.6% of the inoculated larvae of the 3 Trichinella species became adult worms in the small intestines of the host animals. However, in rats, more than 96% of adult worms of all 3 Trichinella species were expelled from the gut by day 10 PI. In gerbils, only 4.8-18.1% of adult worms were expelled by day 10 PI. In accordance with the intestinal worm burden and the persistence of adults, the RCI was the highest in gerbils with values of $241.5{\pm}41.0$ for T. papuae, $432.6{\pm}48$ for T. pseudospiralis, and $528.6{\pm}20.6$ for T. spiralis. Hamsters ranked second and mice ranked third in susceptibility in terms of the RCI, Rats yielded the lowest parasite RCI for all 3 Trichinella species. Gerbils may be an alternative laboratory animal for isolation and maintenance of Trichinella spp.

• Parasites, Hosts and Diseases
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• 제51권3호
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• pp.297-304
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• 2013

Trichinosis is a parasitic zoonosis caused by the nematode Trichinella spiralis. Anthelmintics are used to eliminate intestinal adults as well as tissue-migrating and encysted larvae. This study aimed to investigate the effects of ivermectin and myrrh obtained from the aloe-gum resin of Commiphora molmol on experimental trichinosis. Ninety albino mice were orally infected with 300 T. spiralis larvae. Drugs were tested against adult worms at day 0 and day 5 and against encysted larvae on day 15 and day 35 post-infection (PI). Mature worms and encysted larvae were counted in addition to histopathological examination of muscle specimens. Serum aspartate aminotransferase (AST), alanine aminotransferase (ALT), total protein, albumin, globulin, urea, and creatinine values were estimated. Significant reductions in mean worm numbers were detected in ivermectin treated mice at day 0 and day 5 PI achieving efficacies of 98.5% and 80.0%, while efficacies of myrrh in treated mice were 80.7% and 51.5%, respectively. At days 15 and 35 post-infection, ivermectin induced significant reduction in encysted larval counts achieving efficacies of 76.5% and 54.0%, respectively, while myrrh efficacies were 76.6% and 35.0%, respectively. AST, ALT, urea, and creatinine levels were reduced, while total proteins were increased in response to both treatments compared to their values in the infected non-treated mice. Ivermectin use for controlling T. spiralis could be continued. Myrrh was effective and could be a promising drug against the Egyptian strains of T. spiralis with results nearly comparable to ivermectin.

• Parasites, Hosts and Diseases
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• 제53권5호
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• pp.619-625
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• 2015

The present study was performed to determine the infection status of swamp eels with Gnathostoma sp. larvae in Myanmar. We purchased total 37 Asian swamp eels, Monopterus albus, from a local market in Yangon in June and December 2013 and 2014. All collected eels were transferred with ice to our laboratory and each of them was examined by the artificial digestion technique. A total of 401 larval gnathostomes (1-96 larvae/eel) were detected in 33 (89.2%) swamp eels. Most of the larvae (n=383; 95.5%) were found in the muscle. The remaining 18 larvae were detected in the viscera. The advanced third-stage larvae ($AdL_3$) were 2.3-4.4 mm long and 0.25-0.425 mm wide. The characteristic head bulb ($0.093{\times}0.221mm$ in average size) with 4 rows of hooklets, muscular long esophagus (1.025 mm), and 2 pairs of cervical sacs (0.574 mm) were observed by light microscopy. The average number of hooklets in the 1st, 2nd, 3rd, and 4th rows was 41, 45, 48, and 51, respectively. As scanning electron microscopic findings, the characteristic 4-5 rows of hooklets on the head bulb, a cervical papilla, tegumental spines regularly arranged in the transverse striations, and an anus were well observed. Based on these morphological characters, they were identified as the AdL3 of Gnathostoma spinigerum. By the present study, it has been confirmed for the first time that Asian swamp eels, M. albus, from Yangon, Myanmar are heavily infected with G. spinigerum larvae.