• Korean Journal of Food Science and Technology
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• v.10 no.2
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• pp.136-142
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• 1978

The young cells of Clostridiunm saccaroperbutylacetonicum were rapidly autolysed by exposing them to the hypertonic solution of sucrose(0.3-0.6M) without any other supplement to decompose the rigid cell wall. The cells were converted into the spherical cells by lysis. The spherical cells had following properties: (1) they were absent in the cell wall and osmotically fragile. (2) they were stabilized in the existence of 0.4M sucrose and 5mM $MgSO_4$ (3) they were resistant against adsorption of phage particles. (4) they allowed infection of the isolated phage DNA and produced progeny phage particles. (5) they were able to biosynthesize their macromolecules for a few hours according to a balanced manner of biosynthesis. (6) they were able to produce the bacteriocin particles by mitomycin C treatment. (7) they were unable to multiply. These results were all in the level of typical properties of bacterial protoplasts. It was apparent that the spherical cells formed by lysis occcurring by treatment with hypertonic sucrose were protoplasts.

• Journal of Korean Society for Atmospheric Environment
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• v.33 no.5
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• pp.445-457
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• 2017

In this study, we developed an approach to better account for uncertainties in estimated contributions from fine particulate matter ($PM_{2.5}$) modeling. Our approach computes a Concentration Correction Factor (CCF) which is a ratio of observed concentrations to baseline model concentrations. We multiply modeled direct contribution estimates with CCF to obtain revised contributions. Overall, the modeling system showed reasonably good performance, correlation coefficient R of 0.82 and normalized mean bias of 2%, although the model underestimated some PM species concentrations. We also noticed that model biases vary seasonally. We compared contribution estimates of major source sectors before and after applying CCFs. We observed that different source sectors showed variable magnitudes of sensitivities to the CCF application. For example, the total primary $PM_{2.5}$ contribution was increased $2.4{\mu}g/m^3$ or 63% after the CCF application. Out of a $2.4{\mu}g/m^3$ increment, line sources and area source made up $1.3{\mu}g/m^3$ and $0.9{\mu}g/m^3$ which is 92% of the total contribution changes. We postulated two major reasons for variations in estimated contributions after the CCF application: (1) monthly variability of unadjusted contributions due to emission source characteristics and (2) physico-chemical differences in environmental conditions that emitted precursors undergo. Since emissions-to-$PM_{2.5}$ concentration conversion rate is an important piece of information to prioritize control strategy, we examined the effects of CCF application on the estimated conversion rates. We found that the application of CCFs can alter the rank of conversion efficiencies of source sectors. Finally, we discussed caveats of our current approach such as no consideration of ion neutralization which warrants further studies.

• Journal of Korea Multimedia Society
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• v.14 no.3
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• pp.403-413
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• 2011

In this paper, we proposed a digital image watermarking technique using scrambled binary phase computer generated hologram in the discrete cosine transform(DCT) domain. For the embedding process of watermark. Using simulated annealing algorithm, we would generate a binary phase computer generated hologram(BPCGH) which can reconstruct hidden image perfectly instead of hidden image and encrypt it through the scramble operation. We multiply the encrypted watermark by the weight function and embed it into the DC coefficients in the DCT domain of host image and an inverse DCT is performed. For the extracting process of watermark, we compare the DC coefficients of watermarked image and original host image in the DCT domain and dividing it by the weight function and decrypt it using descramble operation. And we recover the hidden image by inverse Fourier transforming the decrypted watermark. Finally, we compute the correlation between the original hidden image and recovered hidden image to determine if a watermark exits in the host image. The proposed watermarking technique use the hologram information of hidden image which consist of binary values and scramble encryption technique so it is very secure and robust to the various external attacks such as compression, noises and cropping. We confirmed the advantages of the proposed watermarking technique through the computer simulations.

• Korean Journal of Veterinary Service
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• v.40 no.1
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• pp.61-66
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• 2017

Calf diarrhea is a disease experienced by almost all of calves after birth and is one of the representative causes of damage to farmers due to mass mortality and of economic losses to them by inhibiting normal growth. In this study, we conducted quick detection of etiologic agents of diarrhea by using a rapid diagnostic kit to multiply diagnose antigens of five etiologic agents of calf diarrhea (rotavirus, coronavirus, Escherichia coli, Cryptosporidium, Giardia) in Hanwoo (Korean native cattle) calves. When the positive antigen proportion of the calf diarrheal feces for each farm was analyzed, rotavirus, coronavirus, Escherichia coli, Cryptosporidium, and Giardia showed antigen positive rates of 0~67%, 0~20%, 0~60%, 0~20%, and 0~67%, respectively. With regard to the antigen positive rate by age in days after birth, 1-week-old calves showed the antigen positive rate of 20% in rotavirus and 20% in Giardia, and 2-week-old calves showed that of 50% in rotavirus. In addition, 4-week-old calves showed the antigen positive rate of 10% in rotavirus, 10% in coronavirus, 10% in Escherichia coli, and 30% in Giardia, and 8-week-old calves showed the antigen positive rate of 17% in coronavirus, 50% in Escherichia coli, 17% in Cryptosporidium, and 33% in Giardia. Based on the results of this study, the etiologic agents of diarrhea in Hanwoo calves for each farm are widely distributed. Although younger than 2-week-old calves were strongly positive for rotavirus, older than 4-week-old calves were highly positive for Giardia and Escherichia coli. In conclusion, we considered that a rapid diagnostic kit is an effective method for quick detection of etiologic agents and would be helpful for cattle farmers and veterinarians to select appropriate therapeutic method.

• Journal of Korean Society of Transportation
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• v.18 no.4
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• pp.7-18
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• 2000

KS-SIGNAL, a traffic signal optimization model for coordinated arterials, is an optimization model using the mixed integer linear Programming that minimizes total delay on arterials by optimizing left-turn Phase sequences. However, the Previous version of KS-SIGNAL had a difficulty in reducing computation speed because the related variables and constraints multiply rapidly in accordance with the increase of intersections. This study is designed to propose a new model, improving optimizing computation speed in KS-SIGMAl, and evaluate it. This Paper Puts forth three kinds of methodological approaches as to achieve the above goals. At the first step to reduce run-time in the proposed model objective function and a few constraints are Partially modified, which replaces variable in related to queue clearance time with constant, by using thru-movements at upstream intersection and the length of red time at downstream intersection. The result shows that the run-time can be reduced up to 70% at this step. The second step to load the library in LINDO for Windows, in order to solve mixed integer linear programming. The result suggests that run-time can be reduced obviously up to 99% of the first step result. The third step is to add constraints in related to left-turn Phase sequences. The proposed methodological approach, not optimizing all kinds of left-turn sequences, is more reasonable than that of previous model , only in the view of reducing run-tim. In conclusion, run-time could be reduced up to 30% compared with the second results. This Proposed model was tested by several optimization scenarios. The results in this study reveals that signal timing plan in KS-SIGNAL is closer to PASSER-II (bandwidth maximizing model) rather than to TRANSYT-7F(delay minimizing model).

• The Journal of the Korea Contents Association
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• v.11 no.9
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• pp.437-442
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• 2011

The purpose of this study is an measurement of the skin dose of a patient by using the OSLD(optically stimulated luminescent dosimeter) under several irradiation conditions of the X-ray beam for diagnostic radiography. The measurements of skin dose were performed for head, chest, and pelvis. And test of reproducibility was carried out at the chest. As a result, we obtained the skin dose at forehead of head to be 1.30 mSv. The skin doses at xiphoid process, breast and apex of the lung of the chest were acquired 0.92, 0.52 and 0.70 mSv, respectively. And we obtained the skin doses at the left pelvis and the right pelvis to be 2.78 and 3.08 mSv, respectively. As for reproducibility, a coefficient of variation was 0.033. The skin doses were exhibited the values corresponding from 1/100 to 1/17 of the dose limit of the public(50 mSv) at the deterministic effect. In order to make accurate measurements of the skin doses for each tube voltage, the measured values have to multiply by the displayed values of reader by a correction factor. The energy response of the OSLD with the tube voltage will be studied in the near future.

• The Journal of the Institute of Internet, Broadcasting and Communication
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• v.15 no.2
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• pp.269-272
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• 2015

this is the study of the human body antenna device which can detect the powder in a 360-degree on(under) the ground whether it is metal or nonmetal using superhigh frequency RF beam equipped with the body. and it is able to transmit the data of the detection of the powder, battle combats can share that among them. with its flexible roof radial antenna structure, it emits the superhigh frequency RF beam to the front and flank multiply, preprocesses through the powder preprocessing part. and with the non-linear regression model algorism engine part, reflecting the attenuation characteristics depend on the delayed time of degree of the signal power which is received to the superhigh frequency RF beam. so it is able to detect the signal of the most likely mine or powder based on the degree of the answer signal power according to the delayed time of the superhigh frequency RF beam. also, it can detect the powder whether it is metal or nonmetal, mine, dud, VBIED. it can increase the chance of detection about 90% more than existing mine detector.

• Journal of the Institute of Electronics and Information Engineers
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• v.53 no.4
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• pp.59-67
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• 2016

With the enhancement of built-in communication capabilities in various meters and wearable devices, which implies Internet of things (IoT), the demand of small-area embedded processors has increased. In this paper, we introduce a small-area 32-bit pipelined processor, Juno, which is available in the field of IoT. Juno is an EISC (Extendable Instruction Set Computer) machine and has a 2/3-stage pipeline structure to reduce the data dependency of the pipeline. It has a simple pipeline controller which only controls the program counter (PC) and two pipeline registers. It offers $32{\times}32=64$ multiplication, 64/32=32 division, $32{\times}32+64=64$ MAC (multiply and accumulate) operations together with 32*32=64 Galois field multiplication operation for encryption processing in wireless communications. It provides selective inclusion of these algebraic logic blocks if necessary in order to reduce the area of the overall processor. In this case, the gate count of our integer core amounts to 12k~22k and has a performance of 0.57 DMIPS/MHz and 1.024 Coremark/MHz.

• Archives of Plastic Surgery
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• v.33 no.2
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• pp.205-212
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• 2006

Using adipose derived stem cells(ASCs), neurogenic differentiation was induced in a mono layered culture medium containing neuronal induction agents. Cells differentiated to the neuronal cells were observed with a inverted microscope and immunofluorecent study. We made a 15 mm long defect in the sciatic nerve of 14 rats and connected a silicone tube to the defect. Then, we mixed neuronal progenitor cells differentiated from ASCs with collagen gel and grafted them to a group of rats(experimental group) and grafted only collagen gel into another group(control group). In 4 and 8 weeks after the graft, histological observation was made. According to the result, the number and diameter of myelinated axons were significantly increased in the experimental group. In addition, the nerve conduction velocity was improved more in the experimental group and neovascularity also increased. Moreover, reaction with S100 and p75 was observed in regenerated nerves in the experimental group, suggesting that the grafted cells were differentiated into supportive cells such as Schwann's cells. In conclusion, this research proved that ASCs can multiply and differentiate into neuronal cells. If they are grafted into nerve defects, the grafted cells are differ entiated into supportive cells such as Schwann's cells and thus contribute to nerve regeneration. Accordingly, the use of adipose tissue obtained easily without the limitation of donor site can be greatly helpful in treating peripheral nerve defects.

• The Journal of the Korean Society for Microbiology
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• v.12 no.1
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• pp.11-18
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• 1977

A total of 665 strains of Escherichia coli isolated in Korea from stools of patients who were treated with antimicrobial drugs, doctors, and students were tested for the drug resistance and distribution of R plasmids. Approximately 25 to 41% of isolates were resistant to chloramphenicol, tetracycline, streptomycin, sulfisemidine and ampicillin(AP), and 9.5% were resistant to kanamycin. Nalidixic acid-resistant strains were only rarely encountered. The prevalence of resistant strains was significantly higher among patients than doctors and students. Strains multiply resistant to four or more drugs were significantly more prevalent among patient isolates than those from doctors and students, while no difference on the incidence of strains resistant to three or less drugs was noted among isolates from the three groups. The persons carrying strains resistant to four or more drugs were more frequently found among patients than doctors and students. Quite large proportions of drug-resistant strains transferred their resistance to drug-sensitive E. coli, with frequent transfer of whole resistance and AP resistance. Strains having higher multiplicity of resistance showed a tendency toward higher incidence of resistance transfer, irrespective of the origins of strains.