• 한국약용작물학회지
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• 제5권3호
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• pp.186-190
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• 1997

This study was carried out to investigate the effect of polyamines, salt strength. sucrose and gelling agents on the regeneration of plantlets by meristem culture of Aloe arborescens Mill. and Aloe vera L.. Shoot multiplication was more effective when 10mg/ l spermine in Aloe arborescens and 1mg/ l spermidine in Aloe vera added into MS medium than when other polyamines were treated into media. A quarter strength of MS medium was effective for rooting of shoots regenerated. Higher concentration of sucrose (45g/ l) was more effective for shoot regeneration. Addition of 4g/ l gelrite into the medium was effective for induction of multiple shoots from Aloe than that of agar or other concentrations of gelrite. When plantlets regenerated from meristem culture were transferred to pot. survival rate of plantlets was 80% on perlite and was 95% on vermiculite. respectively.

• Journal of Plant Biotechnology
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• 제7권2호
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• pp.135-141
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• 2005

Protocols for in vitro conservation was developed for Coleus forskohlii. Plants maintained both in field served as explant source. Shoot tips and single node cuttings were used to optimize protocols for in vitro multiplication. MS basal medium supplemented with $0.54\;{\mu}M$ naphthalene acetic acid (NAA) and $8.87\;{\mu}M$ benzy-ladenine (BA) induced multiple shoots in shoot tips and nodes. Shoot multiplication was amplified with a gradual decrease of BA concentration, leading to its final omission after 4 months. Concomitant rooting on multiplication media enabled successful establishment extra vitrum. For in vitro conservation studies, experiments were carried out with 2-3 week maintained in vitro plants under standard and reduced culture conditions (SCC, RCC). In vitro plants could be successfully conserved in full strength MS medium (FMS) under SCC for 6 months without subculture with full potential to regenerate, producing viable shoots and nodes. The root production remained unaffected due to conservation, showing high rooting activity in mannitol and low temperature treatments. Preset low temperature (15 and $10^{\circ}C$) and reduction in media constituents does not appear to favour conservation, although the former accomplished conservation levels equal to (FMS) under SCC.

• Journal of Plant Biotechnology
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• 제8권1호
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• pp.15-19
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• 2006

An efficient protocol for in vitro multiple shoot bud induction and plant regeneration from mature green cotyledon derived callus tissues of Acacia sinuata has been developed. Callus formation occurs at all the concentrations of thidiazuron (TDZ) in Murashige and Skoog's (MS) medium, but 0.6 ${\mu}M$ proved to be the best with maximum callus formation frequency. Supplementation of TDZ in combination with indole-acetic acid (IAA) in MS media accelerates shoot bud organogenesis in differentiating callus tissues with 60-70% conversion of shoot buds into shoot Most efficient shoot organogenesis was recorded when TDZ induced calli were subcultured at different concentrations of 6-benzyla-denine (BA). Optimum shoot bud induction and plant regeneration from callus was achieved when 0.6 ${\mu}M$ (TDZ) induced calli were subcultured at 3.0 ${\mu}M$ (BA) where $16.6{\pm}0.74$ shoots/unit callus on obtained. Rooting in in vitro differentiated shoots was achieved when transferred to medium containing different concentration of indole-3-butyric acid (IBA) in full & half strength MS medium. The well rooted plantlets were hardened and transferred to net house with 90% survival rate.

• 한국약용작물학회지
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• 제11권5호
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• pp.397-401
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• 2003

심장질환 및 암치료에 약제로 사용되어지는 D. purpurea의 잎 절편으로부터 신초의 재분화와 증식을 시도하였다. MS 배지에 $2\;mg/l$ BA와 $0.1\;mg/l IAA를 조합하여 처리하였을 때 잎절편으로부터 부정아가 형성되었다. 유기된 부정아로부터 신초의 증식은 식물생장조절물질이 첨가된 $B_5$ 배지에서 배양 5주 후 약 21개의 신초가 분화되었으며, 신초의 길이 신장과 생육은 WPM 배지와 MS 배지에서 가장 양호한 것으로 나타났다. 한편 기내 증식된 신초로 부터의 발근은 $1\;mg/l$ IBA를 첨가된 MS 배지에서 신초당 21.5개가 형성 되었다.

• Journal of Plant Biology
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• 제27권3호
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• pp.139-147
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• 1984

This in vitro study was employed to clarify the effects of several pollutants i.e. $SO_2$, fluoride, cadmium(Cd), aluminum(Al) and NaCl, on the organogenesis and growth responses of shoot-tip, stem and multiple-buds segments derived from hypocotyl or cotyledon culture of petunia seedlings. ${Na_2}{SO_3}$levels of more than 200$\mu{g}$/ml had significantly reduced organogenesis, growth, and chlorophyll content. The injuries caused by ${Na_2}{SO_3}$, concentration of more than 400$\mu{g}$/ml were alleviated by increasing hydrogenion concentration of medium, indicating some relationship between two factors. Organogenesis was not affected by the fluoride concentration up to 100ppm in the media, but the growth and chlorophyll content were greatly reduced by the fluoride. The effect of Cd depended on the explant sources used for the culture; 1.0ppm was effective for fresh weight increase in shoot tip culture, and 3.0ppm in stem segments culture. Organogenesis and growth were greatly reduced by more than 10.0 Cd treatment. Growth and formation of shoots were better with Na conc. of 0.3% compared to control, but those of roots were inhibited. Na concentration goes over 1.0%, organogenesis and subsequent growth were inhibited, and chlorophyll synthesis was drastically reduced. Chlorophyll content was increased on the medium supplemented with Al 50$\mu{g}$/ml compared to control. However the formation and growth of shoots were greatly inhibited with more than 400$\mu{g}$/ml and roots were not produced at all.

• Journal of Plant Biotechnology
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• 제34권4호
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• pp.293-298
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• 2007

Robinia pseudoacacia var. umbraculifera, commonly called umbrella black locust were regenerated after co-cultivation of internode segments with Agrobacterium tumefaciens which included yeast cadmium factor 1 (YCF 1) gene. The tolerance to cadmium and lead for plants can be increased by the YCF1 gene expression. Moreover, the recent studies have shown that YCF1 gene transgenic plants increase the accumulation of cadmium and lead into plant vacuoles. The effect of plant growth regulator such as 2,4-dichlorophenoxyacetic acid (2,4-D), ${\alpha}$-naphthaleneacetic acid (NAA), 6-benzyladenine (BA), and thidiazuron (TDZ) were studied to evaluate the propagation of plants through internode explants. The efficient induction of multiple adventitious shoots and callus were observed on a medium supplemented with 0.1 mg/L TDZ + 0.2 mg/L BA. To induce shoot elongation and rooting, regenerated shoots were transferred into basal MS medium without any plant growth regulator. Successful Agrobacterium tumefaciens mediated transformation was obtained by 20 min vacuum-infiltration with $50{\mu}M$ acetosyringone on the optimal multiple shoot induction medium with 30 mg/L hygromycin and 300 mg/L cefotaxime. To confirm the integration and expression of transgene, Polymerase Chain Reaction (PCR) and Reverse Transcriptase PCR (RT-PCR) were performed with specific primers. The frequency of transformation was approximately 18.94%. This study can be used to genetic engineering of phytoremediator.

• 한국자원식물학회지
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• 제19권3호
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• pp.427-431
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• 2006

In vitro high-frequency plant regeneration of Muscari comosum var. plumosum through somatic embryogenesis was obtained via two developmental pathways: direct embryos and multiple shoots regenerated from embryogenic callus. Flower bud with pedicel, receptacle, petal and ovary wall, floral stalk and leaf as explants were cultured in MS medium supplemented with various plant growth regulators. Embryos formed directly from pedicel, receptacle and floral stalk. Depending on explant sources, the optimal medium was MS medium supplemented with 0.2 mg/L IBA and 0.3 mg/L BA, 3.0 mg/L IBA and 3.0 mg/L BA, and MS-free medium for pedicel, receptacle, and floral stalk, respectively. Multiple shoots regenerated from embryogenic cal]i which was initiated from petal, ovary and leaf were observed in MS medium with different concentrations and combinations of hormone. The most suitable medium for each type of explant was 3.0 mg/L IBA and 3.0 mg/L BA(petal and ovary) and 5.0 mg/L IBA and 5.0 mg/L BA (leaf) Furthermore, the combination of 0.1 mg/L 2,4-D and 1.0 mg/L BA was also good for all sources of explants not only for direct embryo formation, but also, for embryogenic callus induction.

• 농업생명과학연구
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• 제45권1호
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• pp.33-40
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• 2011

An efficient method for the rapid micropropagation of Camptotheca acuminata from axillary buds was established by application of various plant growth regulators. Among various cytokinins, $0.5mg\;L^{-1}$ BA showed the best performance on shoot multiplication, number average multiple shoots up to 10.8. The propagated shoot cuttings in vitro were elongated on NN basal medium without plant growth regulators. The secondary multiple shoots were induced at the site of initially induced buds. Rooting was induced directly near the base of the shoot on half-strength NN medium containing $0.5mg\;L^{-1}$ of IBA, whereas high concentration of $1.0mgL^{-1}$ IBA could induce callus at the base of the shoot. The camptothecin content, anticancer compound of the micropropagated plants was contained in various tissues. Camptothecin contents were 1.8 and $2.5mg\;g^{-1}$ dry weight in stems from propagated in vitro and mother plant, respectively. This result may be used to develop strategies for large-scale propagation of elite C. acuminata trees.

• 한국자원식물학회지
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• 제23권1호
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• pp.7-13
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• 2010

본 실험은 새우난초의 기내 다신초 유도를 통한 재분화 조건의 확립과 이를 통한 토양순화을 목적으로 수행되었다. 우선 새우난초의 유식물체로부터 잎, 구경, 뿌리를 달리하여 부정아을 유도한 결과, 잎 부위는 구경이나 뿌리보다 양호한 부정아의 유도를 보였다. 또한, 부정아 유도시 3.0 mg/L의 BA와 1.0 mg/L의 NAA가 첨가된 배지에서 가장 높은 부정아 유도율을 보였다. 유도된 부정아을 이용하여 $GA_3$의 농도에 따른 다신초 증식률을 조사한 결과, 3.0 mg/L의 $GA_3$가 포함된 배지에서 가장높은 결과를 보였다. 최적의 발근조건을 확인하고자 IBA와 NAA의 농도는 달리하여 실험한 결과, 3.0 mg/L의 NAA가 첨가된 배지에서 가장 높은 발근수와 발근길이를 보였다. 발근이 되어 모본과 유사하게 배양된 새우난초의 유식물체를 모래단용상, 모래와 TKS-II를 1:1로 배합한 ST혼용상, 펄라이트 단용상, 펄라이트와 TKS-II를 1:1로 배합한 PT혼용상, TKS-II단용상을 조성하여 심재한 후 신장률, 줄기직경, 뿌리길이, 생존률을 조사한 결과 모래와 펄라이트에 TKS-II를 1:1로 혼용하여 제조된 배양토에서 가장 양호한 결과를 보였다.

• 한국자원식물학회지
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• 제7권1호
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• pp.17-22
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• 1994

This study was carried out to investigate the optimum medium and concentrations of growth regulators for induction of multiple shoot by meristem culture of floe otorefcenf Mill. MS medium supple-mented with 3${\mu}{\textrm}{m}$ TDZ was effective for induction of multiple shoot. Shoot multiplication was more ef-fective when 2mg/1 BA combined with 0.Img/1 IAA than when only BA were treated on medium. Halfstrength of MS medium supplemented with 2mg/L IAA was effective for rooting of shoots regenerated.When plantlets regenerated from meristem culture were transferred to pot, survival rate of plantletswas 80% on perlite and was 95% on vermiculite, respectively.