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Soil Acclimatization of Calanthe discolor through Multiple Shoot Formation from Tissue Culture  

Bae, Kee-Hwa (Jeju Biodiversity Research Institute, Jeju Hi-Tech Industry Development Institute)
Yoon, Eui-Soo (Department of Biological Science, College of Natural Sciences, Kongju National University)
Yun, Pil-Yong (Jeju Biodiversity Research Institute, Jeju Hi-Tech Industry Development Institute)
Choi, Yong-Eui (Division of Forest Resources, College of Forest and Environmental Sciences, Kangwon National University)
Publication Information
Korean Journal of Plant Resources / v.23, no.1, 2010 , pp. 7-13 More about this Journal
Abstract
This experiment was conducted to establish the micropropagation of Calanthe discolor through multiple shoot formation from the culture of leaf, corm and root explants. Frequency of adventitious shoot formation from leaf explants was higher than those of corms and root explants. Frequency of adventitious shoot formation on medium with various concentrations of BA (0. 1.0, 3.0, and 5.0 mg/L) and NAA (0, 0.1, 0.5, and 1.0 mg/L) was tested. The maximun induction of adventitious shoot was obtained on half strength Murashige and Skoog (MS) medium supplemented with 3.0 mg/L BA and 1.0 mg/L NAA after 6 weeks of culture. Multiple shoots were transferred onto half strength MS medium with various concentrations of GA3 (0, 0.1, 0.5, 1.0, 3.0, and 5.0 mg/L). The number and length of multiple shoots on medium were highest on medium with 3.0 mg/L GA3. All the adventitious shoot grew well and rooted on half strength MS medium with 3.0 mg/L NAA. The plantlets were acclimatized up to 100% on sand with TKS-II or pearlite with TKS-II.
Keywords
Calanthe discolor; adventitious shoot initiation; multiple shoot; soil acclimatization;
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Times Cited By KSCI : 4  (Citation Analysis)
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