Korean Journal of Plant Resources (한국자원식물학회지)

The Plant Resources Society of Korea (한국자원식물학회)
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Somatic Embryogenesis from Various Parts of Muscari comosum var. plumosum

  • Xudong He (The Key Laboratory of Forest Genetics and Gene Engineering, Nanjing Forestry University) ;
  • Ko Jeong-Ae (Faculty of Biological Resources Sciences, College of Agriculture, Chonbuk National University) ;
  • Choi Jeong-Ran (Faculty of Biological Resources Sciences, College of Agriculture, Chonbuk National University) ;
  • Kim Hyung-Moo (Faculty of Biological Resources Sciences, College of Agriculture, Chonbuk National University) ;
  • Kim Myung-Jun (Faculty of Biological Resources Sciences, College of Agriculture, Chonbuk National University) ;
  • Choi So-Ra (Jinan Medicinal Herbs Experiment Station) ;
  • Kim Young-Gon (Faculty of Forest Science, Chonbuk National University) ;
  • Kim Dong-Hee (Interdisciplinary Program of International Tea Culture, Mokpo National University) ;
  • Kim Hyun-Soon (International Technical Cooperation Center Rural Development Administration)
  • Published : 2006.06.01
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Abstract

In vitro high-frequency plant regeneration of Muscari comosum var. plumosum through somatic embryogenesis was obtained via two developmental pathways: direct embryos and multiple shoots regenerated from embryogenic callus. Flower bud with pedicel, receptacle, petal and ovary wall, floral stalk and leaf as explants were cultured in MS medium supplemented with various plant growth regulators. Embryos formed directly from pedicel, receptacle and floral stalk. Depending on explant sources, the optimal medium was MS medium supplemented with 0.2 mg/L IBA and 0.3 mg/L BA, 3.0 mg/L IBA and 3.0 mg/L BA, and MS-free medium for pedicel, receptacle, and floral stalk, respectively. Multiple shoots regenerated from embryogenic cal]i which was initiated from petal, ovary and leaf were observed in MS medium with different concentrations and combinations of hormone. The most suitable medium for each type of explant was 3.0 mg/L IBA and 3.0 mg/L BA(petal and ovary) and 5.0 mg/L IBA and 5.0 mg/L BA (leaf) Furthermore, the combination of 0.1 mg/L 2,4-D and 1.0 mg/L BA was also good for all sources of explants not only for direct embryo formation, but also, for embryogenic callus induction.

Keywords

References

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