Journal of agriculture & life science (농업생명과학연구)

Institute of Agriculture & Life Science, Gyeongsang National University (경상대학교 농업생명과학연구원)
권호 표지

Effects of Plant Growth regulators on Rapid in vitro Propagation of Camptotheca acuminata from Axillary Buds

  • Kang, Seung-Mi (Division of Forest Research, Gyeongsangnam-do Forest Environment Research Institute) ;
  • Min, Ji-Yun (Suncheong Oriental Medicinal Herb Institute) ;
  • Park, Dong-Jin (Division of Environmental Forest Science, Gyeongsang National Univ.) ;
  • Jeong, Mi-Jin (Division of Environmental Forest Science, Gyeongsang National Univ.) ;
  • Song, Hyun-Jin (Division of Environmental Forest Science, Gyeongsang National Univ.) ;
  • Heo, Chang-Mi (Division of Environmental Forest Science, Gyeongsang National Univ.) ;
  • Moon, Hyun-Shik (Division of Environmental Forest Science, Gyeongsang National Univ.) ;
  • Kim, Jong-Gab (Division of Environmental Forest Science, Gyeongsang National Univ.) ;
  • Karigar, Chandrakant S. (Department of Biochemistry, Bangalore Univ.) ;
  • Choi, Myung-Suk (Division of Environmental Forest Science, Gyeongsang National Univ.)
  • Received : 2011.01.03
  • Accepted : 2011.02.22
  • Published : 2011.02.28
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Abstract

An efficient method for the rapid micropropagation of Camptotheca acuminata from axillary buds was established by application of various plant growth regulators. Among various cytokinins, $0.5mg\;L^{-1}$ BA showed the best performance on shoot multiplication, number average multiple shoots up to 10.8. The propagated shoot cuttings in vitro were elongated on NN basal medium without plant growth regulators. The secondary multiple shoots were induced at the site of initially induced buds. Rooting was induced directly near the base of the shoot on half-strength NN medium containing $0.5mg\;L^{-1}$ of IBA, whereas high concentration of $1.0mgL^{-1}$ IBA could induce callus at the base of the shoot. The camptothecin content, anticancer compound of the micropropagated plants was contained in various tissues. Camptothecin contents were 1.8 and $2.5mg\;g^{-1}$ dry weight in stems from propagated in vitro and mother plant, respectively. This result may be used to develop strategies for large-scale propagation of elite C. acuminata trees.

Keywords

Acknowledgement

Supported by : Korea Forest Service

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