• 한국작물학회지
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• 제50권spc1호
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• pp.224-227
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• 2005

뽕나무 오디는 기능성 식품소재 및 농가의 소득을 증진시킬 수 있는 과실로서 뽕나무 묘목에 대한 일반인의 수요가 증가하고 있으나, 현재 오디생산을 위한 뽕 품종은 '대성뽕'외에 등록되어 있지 않다. 따라서 본 연구는 우리나라에 유전자원으로 보존 중인 뽕나무의 결실 오디를 계통별로 채취하여 과실적 특성을 조사하고 우수한 계통을 선발함으로써, 오디 생산용 뽕 품종의 육종 효율을 높이고 품종의 조기 농가보급을 위한 기초 자료를 제공코자 하였다. 그 결과를 요약하면 다음과 같다. 1. 그루당 10kg이상 수확 가능한 계통으로서 '수성뽕', '청노상', '수원노상', '절곡조생(충북)' 4계통을 다수성 계통으로 선발하였다. 또한 단과중이 3.5g 이상인 대과형 계통과 17 Brix $\%$ 이상인 고당도 계통을 각각 5계통, 4계통 선발하였다. 공시계통 중 '절곡조생(충북)'은 단과중이 4.5g으로서 가장 높았으며, '강선'은 19.6 Brix $\%$으로 당도 함량이 가장 높았다. 2. 수량, 단과중 및 당도 값을 동시에 만족시키는 오디 생산용 우량 계통으로서 '절곡조생(충북)', '팔청시평', '강선', '수원노상', '죽천조생', '수성뽕', '당상7호', '장소상' 8계통을 선발하였다.

• 환경생물
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• 제32권3호
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• pp.263-269
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• 2014

농생태계의 뽕나무 경작지에서 서식하고 있는 지렁이상을 확인하기 위하여, 2013년 4월부터 2014년 3월까지 조사를 하였다. 분류된 남방계지렁이는 총 5속 9종으로 다음과 같다. Aporrectodea caliginosa (Savigny, 1826), Bimastos parvus (Eisen, 1874), Eisenia fetida (Savigny, 1826), Drawida japonica (Michaelsen, 1892), Amynthas heteropodus (Goto & Hatai, 1898), Amynthas agrestis (Goto & Hatai, 1899), Amynthas corticis (Kinberg, 1867), Amynthas hilgendorfi (Michaelsen, 1895), Amynthas sp. 등이다. 장미줄지렁이 (Ap. caliginosa) 개체군 밀도 소장은 10월에 정점을 보이고 다음해 2월까지 감소하고, 성체와 미성체는 동시에 출현하며, 그들의 조성 비율은 성체 11.4%, 준성체 12.6 11%, 미성체 76% 이었다.

• 한국잠사곤충학회지
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• 제52권1호
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• pp.64-72
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• 2014

주로 누에사육을 위한 사료 생산용으로 이용되던 뽕나무의 용도가 다양화 되면서, 부산물로 이용되어 온 오디가 새로운 소득작목으로 정착되어 가고 있다. 이에 따라 오디 생산에 적합한 뽕 품종에 대한 수요가 늘어나고 있어, 새로운 조숙계 오디용 뽕품종으로 '수향'을 육성하였으며 이 품종의 육성 경위와 특성은 아래와 같다. 1. '07~'12년까지 수원, 공주, 완주 및 진주 4개 지역에서 '청일'을 대조품종으로 하여 지역적응시험을 거쳤으며, 직무 육성신품종 심의회에서 아래와 같이 오디 생산용으로 우수한 품종임이 인정되어 품종으로 보호 출원하였다. 2. '수향'은 국립농업과학원 잠사양봉소재과 뽕나무 유전자원 포장(수원 소재)에 보존되어 있는 계통 중 오디의 특성이 우수한 '조생홍피상'을 모본으로 하고, '개량뽕'을 부본으로 하여 교배한 계통이다. 3. '수향'은 백상형(Morus alba L.)에 속하는 암나무이며, 발아개엽기는 '청일뽕'과 비슷한 직립성의 중생종이다. 4. '수향'의 잎모양은 타원형으로, 오디 수확시기에 조사한 '수향' 품종의 잎의 기부부분부터 잎의 정단부까지 측정한 잎의 길이는 26.7 cm, 잎의 가장 넓은 부위의 길이를 측정한 옆폭은 19.8 cm로 청일뽕보다 상대적으로 큰 편이다. 5. 오디는 식재 후 3년차부터 결실하기 시작하였으며, 식재 후 3년차('09, 결실 1년차)에는 대조품종인 '청일'과 같은 수준을 나타내었다. 식재 후 4년차('10, 결실 2년차)에는 19%, 5년차('11, 결실 3년차) 14%, 6년차('12, 결실 4년차) 11% 높아, 시험기간 결실 4년간의 평균수량은 '청일' 보다 12% 증수되는 결실성이 우수한 품종이다. 오디 숙기는 '청일' 보다 초숙기 기준 7일정도 빨리 수확되는 조숙계이다. 6. 오디 한 개의 무게인 단과중은 2.5 g으로 '청일' 품종 2.0 g에 비하여 무거운 중과형이며, 육안 관찰에 의한 완숙된 오디의 색상은 검은색으로, 오디의 당도는 '청일'보다 높다.

• International Journal of Industrial Entomology and Biomaterials
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• 제31권2호
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• pp.115-126
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• 2015

The tropical climate prevailing in India adversely affects temperate bivoltine silkworm rearing and causes crop loss especially during summer. Identification of high temperature tolerant bivoltine breeds by screening for thermotolerance in the silkworm, Bombyx mori (Lepidoptera: Bombycidae) is an essential prerequisite for the development of thermotolerant bivoltine breeds / hybrids. Therefore, in this study, 20 silkworm breeds were reared at different temperatures (25 ± 1℃,32 ± 1℃, 34 ± 1℃ and 36 ± 1℃) for 6 h every day from 3^rd d of 5^th instar to till spinning. Significant differences (p < 0.01) were found among all the rearing traits over temperature. Based on pupation percentage, SK4C and BHR3 were identified as thermotolerant bivoltine breeds. Hierarchical clustering analysis based on rearing traits at tested temperatures grouped 20 silkworm breeds in four clusters which included one cluster each of susceptible and tolerant, and two clusters of moderately tolerant silkworm breeds. This suggests that clustering based on rearing data at high temperatures by using Euclidean distance can be an effective approach in classifying the silkworm breeds on their thermotolerance capacity. The identified breeds would be used for development of thermo tolerant bivoltine silkworm breeds / hybrids.

• International Journal of Industrial Entomology and Biomaterials
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• 제13권2호
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• pp.85-95
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• 2006

Seveval Chinese and Japanese varieties with good characters were used in the breeding. After 5 years (15 generations), a pair of robust and high quality silkworm variety with NPV resistance was bred by means of a combination of crossing and pedigree selection complemented by the selection of NPV resistance. The variety was identified jointly nationwide in 2003 and 2004, and appraised by National Mulberry and Silkworm Appraising Committee. Results are as follows: its cocooning rate is over 93%, shell rate 23-25%, filament length 1200-1300 meters, reelability 75-88%, Length of non-broken cocoon filament 900-1100 meters, raw silk rate 17-19%, neatness 95-97 points, and cocoon crop, cocoon shell weight and raw silk weight per 10000 larvae is higher than those of the control variety by 7-10%, 14-19% and 14-18%, respectively. The variety is not only robust, resistant to high temperature and NPV, easy to rear, uniform in hatching, molting and maturing, but also lays more eggs, and its fecundity is high. It is suitable to rear in the Yangtze River Basin, the Yellow River basin and the Pearl River basin of China.

• 한국잠사학회:학술대회논문집
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• 한국잠사학회 1997년도 Progress and Future Development of Sericultural Science and Technology 40th Anniversary Commemoration Symposium
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• pp.1-21
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• 1997

Silk is praised "Queen of Fiber", which wrote a glorious page in the history of human adornment culture. Thanks to the development of science and technology, silk production has been increased dramatically during last half century. Nowadays, as "Healthy Fiber" silk lets all the globe to be forming the "Silk Boom" Like in other scientific fields, a great deal of progress has been made in sericultural science and technology, which also accelerated the development of multipurpose utilization of sercultural recourses. The main tendency of the sericulture development is raising the quality of cocoon, and at the same time, increasing the output per unit mulberry area and labor production efficiency. The chief measure of raising the cocoon quality is breeding and selecting the new mulberry and silkworm varieties, preventing and curing the diseases and pests and improving the environment of mounting, and the symble for increasing the labor productivity is carrying out the technological system of saving labor and operation ease, and enlarging the production scale. As a natural protein fiber, silk will be still a favorite with human in the next century, because its structure and properties can not be replaced by other any fiber. The 21st century is the biological time, and measures of developing sericulture will use the achievement on the field of molecular biology and biotechnology to carry out the molecular breeding, development regulating, silkworm rearing industrializing, exploiting the new silk and its new usage, utilizing the sericultural resources, and producing the special protein from silkworm as a host of expressing exogenous genes so that make the sericulture to contribute more what it can do for the human being.

• International Journal of Industrial Entomology and Biomaterials
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• 제42권2호
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• pp.33-45
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• 2021

Many researchers are using the heterosis, heterobeltiosis and hybrid vigour over check parent value formulae to determine the hybrid vigour for animals, plants and silkworm breeding. These formulae are ideal for determine the hybrid vigour for the positive direction of single trait. It is difficult using these formulae for multiple traits. Suggested modification for cardinal formulae were made as well as suggestion new formula for determines hybrid vigour for multiple traits. Modifications of hybrid vigour were made to facilitate judgment of best hybrids under study for multiple traits. Nineteen local hybrids of mulberry silkworm were prepared for these investigations in addition the imported Bulgarian hybrid. Comparison between the cardinal and the modifications formulae were applied for thirteen economic characters. Nine positive and four negative direction characters were observed. Modified formulae make the judgment of heterobeltiosis, heterosis and hybrid vigour over check parent value very facilitate for positive and negative traits.

• 한국잠사곤충학회지
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• 제2권
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• pp.1-31
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• 1962

본 연구는 견사중의 Cystine 성분을 가잠전과정을 통하여 기존재를 확인하고 견사구조중에서 cross linkage를 조성하고 있음을 확인하는데 있으며 다음과 같은 결론을 얻었다. 1. Cystine 결정체를 Folin 씨 방법으로 견사로부터 분리하였다. 2. 견사 및 그와 관련있는 과정에서 Sullivan 씨 방법으로 다음과 같이 분석하였다. 시료 Cystine 함유량 ％ A. 상엽단백질 0.175 B.잠란 0.33 C. 잠체 (탈지, 숙잠, 견사선제외) 0.41 D. 견사선 (숙잠) 1.23 E. 잠 0.0 F. 잠통 (탈지) 0.30 G. 잠아 (탈지) 0.60 H. 생계 0.22 I. Fibroin 0.175 J. Sericin 0.30 3. 잠체내의 Cystine 존재는 상엽에서 0.175%, 견사선에서 0.12%의 Methionine을 확인함으로 생화학적의의와 더불어 재확인하였다. 4. 견사선내의 Sulfhydryl 화합물은 Sericin과 Methionine으로 전환하는 동시에 전자는 액상견으로 분비되어 견사를 구성하고 후자는 영양목적으로 견사선세포 육성에 사용되는 것으로 믿어진다. 5. Cystine 성분량은 상품종, 잠품종, 자웅성, 육종과정과 사육환경에 따라 변화한다. 6. 잡종강세로 육성된 교잡종은 원잠종보다 효과적인 성장을 위하여 더욱 많은 영양성 아미노산을 필요로 한다. 7. 여기한 관찰을 통하여 견사중 아미노산조성은 어느정도 변화하는 것이며 Cystine은 섬유의 무정형부분 특히 Sericin에 함유되고 있다. 8. 순수한 Cystine 또는 양모 Cystine을 첨식한 결과 하등의 이익점을 초래하지 못하였음으로 상엽중에 함유되어있는 Cystine과 Methionine 함유량은 잠아의 영양생리에 충분한 것으로 보인다. 9. 견사중의 disulfide cross linkage는 Harris 씨 방법과 여러가지 염류와 산에 의한 수축현상으로 확인하였고 양모와 대조하여 시험하였다. 10. 본 연구중 견사의 사상구조와 액상견의 입망상구조를 현미경으로 포착하였다. 액상견의 입망상은 입상 peptide의 OH ion과 물의 H ion의 인력관계로 형성되고 있다는 것을 기 film의 배열상태로 고찰하게 되었고 상엽단백질의 입망상은 견사의 사상형성에 적당한 사료임을 보이고 있다. 11. 본 논문을 통한 Cystine 존재는 비록 그 량이 적지마는 견사중의 Cystine 전무에 대한 종래관념을 전환시킬 것이다.

• 한국잠사곤충학회지
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• 제13권2호
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• pp.123-133
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• 1971

The author is the first man who tried to use an anesthetic on insect specially for silkworm in orde to evaluate the silkworm health and silk yielding ability and the obtained results are as followings. 1. The necessary ether vapor induction for narcosis on silkworms is varied by the glowth of the silkworm which the larger worm is, the longer induction is required. For instance, it was 2∼3 minutes for the worms of third day fifth instar silkworm in case use of ether anesthetic. 2. The longer anesthetic induction for silkworms, the longer recovery needs from anesthesia. In case five minutes ether vapor induction, silkworms recovered narcosis during in 5∼130 minutes which had varied very much by the health variation. 3. The ether induction caused silkworm to vomit digestive juice from a few per cent of the worms, but the chloroform induction showed majority of the worms to vomit the digestive juice out of mouth. So, the ether was found as better anesthetic for silkworms. 4. When ether induction last more than 20∼30 minutes, the recovered silkworms can eat mulberry, but when it gets more than three hours they can not eat mulberry. And when it last more than ten minutes, the silkworm may eat mulberry leaf, but they can not spinn cocoon properly. 5. In case five minutes ether induction for silkworms on third day fifth instar, the stronger variety is, the rallier recovered from narcosis. 6. The recovering duration from narcosis varies regarding with each worm health which shows Poisson′s distribution even in a same variety silkworm. 7. The female worms recover from narcosis earlier than male worms which means the female worm is stronger than male one. 8. The later recovered silkworm from narcosis spinned more rich cocoon silk and ended with smaller pupae weight. Such a tendency showed until at some recovery duration, then the silk yield droped down on the worms recovered in more longer duration. The author (Choe) had named such a relation curve as "Silk Yield Curve against Silkworm Health." 9. The silk yield or cocoon layer ratio had varied from 13 to 27% even in a same worm varity cocoon which showed serious variation and call attention carefulness for the duplication work of a variety silkworm eggs. 10. Not always the rich silk yielding worm is the best worm during the silkworm selection and it should be considered with the silkworm health evaluation. 11. At present situation, only specific breeding expert is allowed to join in the selection service because of need many years experience by use of visual observation, but the ether anesthesia method may help such an evaluation with more accuracy and easy way even for the people in fresh on the field. 12. The effect of the narcosis on the silkworm for the next generation or hybrid worm will be reported in next publication.

• 한국잠사곤충학회지
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• 제17권1호
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• pp.63-68
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• 1975

Flacherie of all other silkworm diseases greatly affects cocoon crop as it is far-reaching and wide spreading. Fleacherie which kills silkworms caused by bacteria can be classified as bacterial digestive organ disease, and "Sotto" disease. Bacterial digestive organ disease is caused by the bacteria living in the silkworms alementary canal and a majority of flacherie belongs to this disease. Septicemia is caused by bacterias breeding in silkworms body fluid but its attach is comparatively limited during the larva period. "Sotto" disease is caused by eating mulberry leaves infected with bacteria which produce toxin and silkworms are intoxicated and killed by the toxin. The cause of flacherie is mainly due to a poor environment. The unclean and unsanitary silkworm rearing beds help bacterias breeding and bacteria enter silkworms body through mouth organ or skin. The present study is to investigate various causes of flacherie by means of pulverization of silkworm. Filtrated fluid is extracted by centrifuge and hypodermic of peroral inoculation-is given to young and medium silkworms of spring and autumn. The gained results of the experiment are summarized as follows： 1. Silkworms infected with flacherie were pulverized and their filtrated fluid was extracted by centrifuge and inspected under microscope to find polyhedron from the fluid. 2. The experimenting group of peroral inoculation. a) From the third day of peroral inoculation silkworms appetite generally decreased and ate less compared with the control group. b) After 7 days of the inoculation silkworms suffered from empty head, loose bowels and fainting. c) Some of the silkworms still ate but as were shown in Fig. 3 and 4 some dwarfish silkworms were found. d) There was no remarkable difference between 1st and 2nd instar inoculation groups. e) There was a tendency that the number of diseased silkworms was decerased as the increase of instars. 2. The experimental group of hypodermic inoculation a) Both of 3rd and 4th instar inoculation groups showed no remarkable singularity and the number of diseased silkworms decreased. b) The rate of diseased silkworms was comparatively low because the body fluid was acidy or toxin was hard soluble. Hypodermic inoculation could not give much harm to the silkworms compared to peroral inoculation.