• Environmental Analysis Health and Toxicology
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• v.18 no.1
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• pp.15-19
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• 2003

Metallothionein gene expression activity of cadmium was investigated in a human lung epithelial cell line. Cells, grown to near confluence, were exposed to 0∼10 ${\mu}$M Cd metal for 6 hours. Cadmium did not cause morphological alteration in lung epithelial cells that are characteristic of cell damages such as cell shrinkage, detachment of the cell from its neighbors, cytoplasmic and chromatic condensation. However, metallothionein genes of MT-1 and MT-2 were rapidly induced in the treated cell measured by RT-PCR. Regarding the induction pattern of motallothionein mRNA, MT-1 mRNA was induced in a dependent manner. MT-2 mRNA induction, which was measured using oligo primers based on cDNA of human reticulocytes, seemed to be slightly increased in low doses but decreased at high concentration used in the experiment.

• BMB Reports
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• v.36 no.4
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• pp.379-386
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• 2003

A mutant herpes simplex virus 1, mtHSV, was constructed by inserting the E. coli beta-galactosidase gene into the loci of icp34.5, the apoptosis-inhibiting gene of HSV. The mtHSV replicated in and lysed U251 (human glioma cells), EJ (human bladder cells), and S-180 (mice sarcoma cells), but not Wish (human amnion cells) cells. With its intact tk (thymidine kinase) gene, mtHSV exhibited susceptibility to acyclovir (ACV), which provided an approach to control viral replication. An in vivo test with mtHSV was conducted in immune-competent mice bearing sarcoma S-180 tumors, which were treated with a single intratumoral injection of mtHSV or PBS. Tumor dimensions then were measured at serial time points, and the tumor volumes were calculated. Sarcoma growth was significantly inhibited with prolonged time and reduced tumor volume. There was microscopic evidence of necrosis of tumors in treated mice, whereas no damage was found in other organs. Immunohistochemical staining revealed that virus replication was exclusively confined to the treated tumor cells. HSV-1 DNA was detected in tumors, but not in the other organs by a polymerase chain reaction analysis. From these experiments, we concluded that mtHSV should be a safe and promising oncolytic agent for cancer treatment.

• Environmental Analysis Health and Toxicology
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• v.19 no.3
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• pp.261-269
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• 2004

Although the biological functions of metallothioneins (MTs) are still being investigated, they have been suggested to be involved in detoxification of heavy metals, scavenging of free radicals, and protection against alkylating agents. MTs have been reported to be induced in most of animal tissues by heavy metals such as zinc, copper, mercury and cadmium, and the proteins have binding affinities to the metals. However, the presence or induction of MTs was reported not to be clear in leydig cells, which produce testosterone for the maturation of spermatozoa in male testes. In this study, we investigated the inducibility of metallothionein isomers by cadmium in cultured mouse leydig cells. Total RNA was extracted from the near confluent grown leydig cells and RT-PCR was Performed using the Primers which were synthesized on the basis of MT-1, 2, 3 and 4 cDNA from GenBank database. As results, MT-1 and MT-2 mRNA were found to be expressed in cadmium non-treated control cells and MT 1 mRNA expression was dose-dependent when leydig cells were treated with cadmium chloride. But MT-3 which is known to be brain specific and MT-4 which is another isoform of metallothionein, were not expressed. Other genes induced or depressed in cadmium treated leydig cells were also identified by microarray techniques.

• The Korean Journal of Malacology
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• v.31 no.3
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• pp.249-252
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• 2015

An aeolid nudibranch species Flabellina athadona (Bergh, 1875) is newly recorded from Korean waters. This species is distinguished from other congeneric species by the following characteristics: translucent white body, deep orange colored digestive glands, plenty of white specks, and a reversed Y-shaped marking on the head between rhinophores and oral tentacles. In this report, we also provide an identification key for the genus Flabellina in Korea, photographs, and diagnostic characteristics of F. athadona. Molecular identification using mtDNA cox1 partial sequences is also provided to help differentiate F. athadona from some of its closely related congeneric species.

• Journal of Species Research
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• v.13 no.3
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• pp.326-328
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• 2024

The beetle family Georissidae, represented by Georissus kurosawai Nakane, is identified for the first time in Korea. Worldwide, the family Georissidae Laporte contains 83 described species in the only genus Georissus Latreille. This beetles usually inhabits sandy or muddy banks of streams, rivers, and standing water, and some species live in tidal flats and leaf litter. Mostly, adults are covered by a layer of mud or sand grains dorsally and are attracted to light. They are characterized by the combination of the following characters: prosternum reduced, procoxae enlarged, metacoxae separate, ventral hydrofuge pubescence absent, elytra punctatostriate and without scutellary stria, metathoracic anepisterna concealed, and sternite III enlarged. Habitus and aedeagus photograph, mtDNA sequence, and habitat information are provided.

• 보존과학연구
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• s.24
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• pp.153-167
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• 2003

We performed nuclear DNA typing and mitochondrial DNA sequencing analysis based on PCR from an ancient Korean remainsexcavated from Siheung in Korea. 7 bones were collected and partially STR(short tandem repeat) systems, Sex determination Amelogenin kit(Promega co, USA), were used in this study. Mitochondrial DNAs were also amplified and sequenced by ABI 310 DNA sequencer. We know that sample no. 2 and no. 3 were females and also sample no. 2 and no.7 possessed the same maternal inheritance by mitochondrial DNA sequencing results. Throughout this research, the mitochondrial DNA sequencing of human in the middle of Joseon Dynasty in Korea is obtained. In addition, this finding will be an important foundation for the future research.

• Animal Systematics, Evolution and Diversity
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• v.17 no.1
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• pp.49-57
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• 2001

The partial sequences of mtDNA cytochrome b gene in two subspecies of striped field mouse(Apodemus agrarius coreae and A. agrarius manchuricus) from Korea and northeast China were analyzed to determine the degree of genetic diversity in ech subspecies and to confirm their subspecific difference. In 18 specimens of A. agrarius coreae, ten haplotypes were resulted, and in two specimens of A. agrarius manchuricus. one haplotype was revealed. Tamura-Nei nucleotide distance among ten haplotypes in subspecies coreae ranged 0.36 to 1.86%. and nucleotide distance between two subspecies (coreae and manchuricus) was 0.37 to 1.47%: maximum infrasubspecific divergence in coreae was greater than maximum intersubspecific difference between two subspecies. Moreover, no major subgroup was resulted when 11 haplotypes in two subspecies were compared. Our sequence result was not cancordant with the morphological data studied so far, and it is concluded that cytochrome b gene sequence is not a good genetic marker to distinguish two subspecies of A. agrarius. In futurem, mtDNA control region analyses seemded to be necessary to reveal genetic relationship between these two subspecies of A. agrarius.

• Korean Journal of Ichthyology
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• v.31 no.4
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• pp.187-194
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• 2019

This study was conducted to know the genetic diversity and population structure of Japanese halfbeak (Hyporhamphus sajori) in the Northeast Asia, using mitochondrial DNA control region. In the present study, a total of 70 individuals were collected from three locations of China (Liaoning), Korea (Tongyeong) and Japan (Wakasa Bay), and 47 individuals sequences from three locations of Japan (Wakasa Bay, Toyama Bay and Mikawa Bay) were downloaded from genbank. A total of 7 haplotypes were identified with 7 polymorphic sites from 358 bp length sequences. Haplotype and nucleotide diversity were very low and ranged from 0 to 0.295±0.156 and 0 to 0.0009±0.0011, respectively. Ancestral haplotype was shared by 94% individuals. An extremely low haplotype and nucleotide diversity, and starlike minimum spanning tree indicated that the species have undergone a recent population expansion after bottleneck. Pairwise F_ST values were low and there was no significant differences among populations suggesting a gene flow among the populations. Dispersal of the eggs with the aid of drifting seaweed and currents might be the major responsible factor for the genetic homogeneity.

• Animal Systematics, Evolution and Diversity
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• v.10 no.2
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• pp.231-243
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• 1994

Samples of Siberian chipmunks (Tamias sibiricus borberi ) from Korea were analyzed by multivariate, air-drying , and blot-hybridization methods in order to determine the degree of variations of morphological characters, chromosomal karyotype, and mtDNA restriction fragment patterns, respectively, Moreover, morphometric characters of samples of Siberian chipmunks from Manchuria(T.sibiricus orientalis) were also analyzed in order to clarify the taxonomic status of the subspecies barberi. Siberian chipmunks from six localities in Korea were in morphometric characters more or less similar with one another and Siberian chipmunks from Manchuria were not so distinct enough to from a subgroup. Siberian chipmunks from Four localities in Korea were identical in their karyotypes (2n=38) : samples from three localities in Korea were nore or less similar in their mtDNA fragment patterns. Siberian chipmunks from Korea appeared to be a omogeneous population and as noted by Corbet (1978) subspecies barberi is the synonym of subspecies orientalis. The scientific name of Siberian chipmunks from Korea and Manchuria is T. sibiricus orientalis, although systematic studies with the samples from North korea, Manchuria and China will be necessary for their taxonomic reconsideration.

• Research in Plant Disease
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• v.11 no.1
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• pp.56-65
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• 2005

To establish taxonomic system of morphologically similar species of small-spored Alternaria, phylogenetic analysis of internal transcribed spacer (ITS 1, ITS 2 and 5.8S rDNA) and mitochondrial small subunit (mt SSU) rDNA sequences and URP-PCR fingerprinting analysis from 11 species ofAlternaria were performed. Phylogenetic analysis of ITS and mt SSU rDNA sequences revealed that 10 out of 11 species of the smallspored Alternaria were phylogenetically identical with a bootstrap value of 100%. A. infectoria only was phylogenetically differentiated from the other species. The results suggest that the 10 small-spored Alternaria species are very closely related evolutionally and the markers can not be used for differentiation of the smallspored Alternaria species. URP-PCR fingerprinting analysis from eleven species of smallspored Alternaria using 10 URP primers showed that it was possible to differentiate the species, although genetic similarities were found among the species. The Alternaria sp. from common pokeweed could be distinguished from other species by URP-PCR analysis, and it was considered as a new species. A. infectoria could be easily distinguished from the other 10 species by phylogenetic analysis of ITS and mt SSU rDNA sequences and the URPPCR fingerprinting analysis.