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http://dx.doi.org/10.5423/RPD.2005.11.1.056

Molecular Characterization of Small-Spored Alternaria Species  

Kim, Byung-Ryun (Crop Environment and Biotechnology, National Institute of Crop Science, RDA)
Park, Myung-Soo (Department of Applied Biology, College of Agriculture and Life Sciences, Chungnam National University)
Cho, Hye-Sun (Department of Applied Biology, College of Agriculture and Life Sciences, Chungnam National University)
Yu, Seung-Hun (Department of Applied Biology, College of Agriculture and Life Sciences, Chungnam National University)
Publication Information
Research in Plant Disease / v.11, no.1, 2005 , pp. 56-65 More about this Journal
Abstract
To establish taxonomic system of morphologically similar species of small-spored Alternaria, phylogenetic analysis of internal transcribed spacer (ITS 1, ITS 2 and 5.8S rDNA) and mitochondrial small subunit (mt SSU) rDNA sequences and URP-PCR fingerprinting analysis from 11 species ofAlternaria were performed. Phylogenetic analysis of ITS and mt SSU rDNA sequences revealed that 10 out of 11 species of the smallspored Alternaria were phylogenetically identical with a bootstrap value of 100%. A. infectoria only was phylogenetically differentiated from the other species. The results suggest that the 10 small-spored Alternaria species are very closely related evolutionally and the markers can not be used for differentiation of the smallspored Alternaria species. URP-PCR fingerprinting analysis from eleven species of smallspored Alternaria using 10 URP primers showed that it was possible to differentiate the species, although genetic similarities were found among the species. The Alternaria sp. from common pokeweed could be distinguished from other species by URP-PCR analysis, and it was considered as a new species. A. infectoria could be easily distinguished from the other 10 species by phylogenetic analysis of ITS and mt SSU rDNA sequences and the URPPCR fingerprinting analysis.
Keywords
ITS; Mitochondrial small subunit; Small-spored Alternaria; URP-PCR;
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