Environmental Analysis Health and Toxicology

The Korean Society of Environmental Health and Toxicology (환경독성보건학회)
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Expression of Metallothionein mRNA in Cadmium Treated A549 Cell Line Derived from Human Lung Epithelial Cell

인간 폐포세포 유래 A549세포주에서의 Cadmium 처리에 의한 메탈로치오닌 유전자 발현

  • 박광식 (동덕여자대학교 약학대학) ;
  • 구자민 (성균관대학교 약학부)
  • Published : 2003.03.01
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Abstract

Metallothionein gene expression activity of cadmium was investigated in a human lung epithelial cell line. Cells, grown to near confluence, were exposed to 0∼10 ${\mu}$M Cd metal for 6 hours. Cadmium did not cause morphological alteration in lung epithelial cells that are characteristic of cell damages such as cell shrinkage, detachment of the cell from its neighbors, cytoplasmic and chromatic condensation. However, metallothionein genes of MT-1 and MT-2 were rapidly induced in the treated cell measured by RT-PCR. Regarding the induction pattern of motallothionein mRNA, MT-1 mRNA was induced in a dependent manner. MT-2 mRNA induction, which was measured using oligo primers based on cDNA of human reticulocytes, seemed to be slightly increased in low doses but decreased at high concentration used in the experiment.

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References

  1. Coyle P, Philcox JC, Carey LC and Rofe AM. Metallothionein: The multipurspose protein. Cellular and Molecular Life Science. 2002; 59: 627-647 https://doi.org/10.1007/s00018-002-8454-2
  2. Dally H, Hartwig A. Induction and repair inhibition of oxidative DNA damage by nickel (II) and cadmium (II) in mammalian cells. Carcinogenesis 1997; 18: 1021-1026 https://doi.org/10.1093/carcin/18.5.1021
  3. Eneman JD, Potts RJ, Osier M, Shukla GS, Lee CH, Chiu J-F. Suppressed oxidant-induced apoptosis in cadmium adapted alveolar epithelial cells and its potential involvement in cadmium carcinogenesis., Toxicology 2000; 147: 215-228 https://doi.org/10.1016/S0300-483X(00)00215-8
  4. Gong Q, Hart BA. Effect of thiols on cadmium-induced expression of metallothionein and other oxidant stress genes in rat lung epithelial cells, Toxicology 1997; 119: 179-191 https://doi.org/10.1016/S0300-483X(96)03608-6
  5. Hart BA, Lee CH, Shukla GS, Shulka A, Osier M, Eneman, JD, Chiu J-F. Characerization of cadmium-induced apoptosis in rat lung epithelial cells: evidence for the participation of oxidant stress. Toxicology 1999; 133: 43-58 https://doi.org/10.1016/S0300-483X(99)00013-X
  6. Hartmann A, Speit G. Effect of arsenic and cadmium on the persistence of mutagen-induced DNA lesions in human cells. Environ. Mol. Mutat. 1996; 27: 98-104 https://doi.org/10.1002/(SICI)1098-2280(1996)27:2<98::AID-EM4>3.0.CO;2-A
  7. IARC, Beryllium, Cadmium, Mercury, and Exposure in the glass manufacturing Industry. IARC Monograph on the evaluation of carcinogenic risk to humans. 1993; 58: 119-238
  8. Kenaga C, Cherian MG, Cox C and Oberdorster G. Metallothionein induction and pulmonary responses to inhaled cadmium chloride in rats and mice, Fund. Appl. Toxicol. 1996; 30: 204-212 https://doi.org/10.1006/faat.1996.0057
  9. Klassen CD, Liu J and Choudhuri S. Metallothionein: an ntracellular protein to protect against cadmium toxicity, Annu. Rev. Pharmacol. Toxicol. 1999; 39: 267-294 https://doi.org/10.1146/annurev.pharmtox.39.1.267
  10. Lambert E, Kille P and Swaminathan R. Cloning and sequencing a novel metallothionein I isoform expressed in human reticulocytes, EEBS lett. 1996; 389: 210-212
  11. McKenna IM, Bare RM and Waalkes MP. Metallothionein gene expression in testicular interstitial cells and liver of rats treated with cadmium. Toxicology 1996; 107: 120-130
  12. Page AL, EI-Amamy MM, Chang AC. Cadmium in the environment and its entry into terrestrial food chain crops. In: Foulkes, E.C. (Ed), Cadmium. SpringerVerlag. New York, 1986; 33-74
  13. Thun MJ, Schnorr TM, Smith AB, Halperin WE and Lemen RA. Motality among a cohort of U.S. cadmium production workers - An update, J. Natl. Cancer Inst. 1985; 74(2): 325-333
  14. Vallee BL. The function of metallothionein. Neurochem. Int., 1995; 27: 23-33 https://doi.org/10.1016/0197-0186(94)00165-Q
  15. Virengo A, Burlando B, Ceratto Nand Panfoli I. Antioxidant role of metallothionein: a comparative overview, Cell Mol. BioI. 2000; 46: 407-417
  16. Yang C-F, Shen H-M, Shen Y, Zhung Z-X, Ong C-N. Cadmium-induced oxidative cellular damage in human fetal lung fibroblasts (MRC-5 cells), Environ. Health Perspect. 1997; 105: 712-716 https://doi.org/10.2307/3433726