• Title/Summary/Keyword: model B3

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Removal of Chromium (VI) by Escherichia coli Cells Expressing Cytoplasmic or Surface-Displayed ChrB: a Comparative Study

  • Zhou, Xiaofeng;Li, Jianghui;Wang, Weilong;Yang, Fan;Fan, Bingqian;Zhang, Chenlu;Ren, Xiaojun;Liang, Feng;Cheng, Rong;Jiang, Fengying;Zhou, Huaibin;Yang, Juanjuan;Tan, Guoqiang;Lyu, Jianxin;Wang, Wu
    • Journal of Microbiology and Biotechnology
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    • v.30 no.7
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    • pp.996-1004
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    • 2020
  • Various genetically engineered microorganisms have been developed for the removal of heavy metal contaminants. Metal biosorption by whole-cell biosorbents can be enhanced by overproduction of metal-binding proteins/peptides in the cytoplasm or on the cell surface. However, few studies have compared the biosorption capacity of whole cells expressing intracellular or surface-displayed metal-adsorbing proteins. In this study, several constructs were prepared for expressing intracellular and surface-displayed Ochrobactrum tritici 5bvl1 ChrB in Escherichia coli BL21(DE3) cells. E. coli cells expressing surface-displayed ChrB removed more Cr(VI) from aqueous solutions than cells with cytoplasmic ChrB under the same conditions. However, intracellular ChrB was less susceptible to variation in extracellular conditions (pH and ionic strength), and more effectively removed Cr(VI) from industrial wastewater than the surface-displayed ChrB at low pH (<3). An adsorption-desorption experiment demonstrated that compared with intracellular accumulation, cell-surface adsorption is reversible, which allows easy desorption of the adsorbed metal ions and regeneration of the bioadsorbent. In addition, an intrinsic ChrB protein fluorescence assay suggested that pH and salinity may influence the Cr(VI) adsorption capacity of ChrB-expressing E. coli cells by modulating the ChrB protein conformation. Although the characteristics of ChrB may not be universal for all metal-binding proteins, our study provides new insights into different engineering strategies for whole-cell biosorbents for removing heavy metals from industrial effluents.

Formal Models and Algorithms for XML Data Interoperability

  • Lee, Thomas Y.;Cheung, David W.
    • Journal of Computing Science and Engineering
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    • v.4 no.4
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    • pp.313-349
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    • 2010
  • In this paper, we study the data interoperability problem of web services in terms of XML schema compatibility. When Web Service A sends XML messages to Web Service B, A is interoperable with B if B can accept all messages from A. That is, the XML schema R for B to receive XML instances must be compatible with the XML schema S for A to send XML instances, Le., A is a subschema of B. We propose a formal model called Schema Automaton (SA) to model W3C XML Schema (XSD) and develop several algorithms to perform different XML schema computations. The computations include schema minimization, schema equivalence testing, subschema testing, and subschema extraction. We have conducted experiments on an e-commerce standard XSD called xCBL to demonstrate the practicality of our algorithms. One experiment has refuted the claim that the xCBL 3.5 XSD is backward compatible with the xCBL 3.0 XSD. Another experiment has shown that the xCBL XSDs can be effectively trimmed into small subschemas for specific applications, which has significantly reduced the schema processing time.

Mathematical Modeling for Estimation of Heart Work (심장 일의 측정을 위한 수학적 모델링)

  • Suh, Sang-Ho;Kaptan, Yalin;Roh, Hyung-Woon;Song, Ji-Hoon
    • Transactions of the Korean Society of Mechanical Engineers B
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    • v.36 no.2
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    • pp.145-151
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    • 2012
  • Evaluation of the heart work is starting to emerge as a new diagnostic tool for arterial diseases. The aim of this study is to develop a mathematical model for the estimation of heart work utilizing the pulse waves between two points of a vessel. In order to calculate heart work, medical data such as blood pressure waveforms (which are measured using a cuff) are utilized. The heart work is calculated by employing the modified Windkessel model together with the viscosity models of Casson or Herschel-Bulkely (H-B). The results indicate that the compliance values at the proximal and distal locations differ for the Casson and H-B models.

Types of Business Models of Business to Business Electronic Commerce (기업간 전자상거래 비즈니스 모델유형에 관한 고찰)

  • 김영상;용세중
    • Proceedings of the Technology Innovation Conference
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    • 2002.02a
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    • pp.269-296
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    • 2002
  • This paper is an exploitative research on the types of B2B e-business model. The research method adopted is a literature review of academic articles, reports of consulting firms, technical papers and seminar materials. This paper presents an integrated business model of business to business electronic commerce. The B2B business model comprises of four dimensions:1) type of operators 2) Product scope(industry scope) 3) transaction method 4) revenue source.

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3D Object's shape and motion recovery using stereo image and Paraperspective Camera Model (스테레오 영상과 준원근 카메라 모델을 이용한 객체의 3차원 형태 및 움직임 복원)

  • Kim, Sang-Hoon
    • The KIPS Transactions:PartB
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    • v.10B no.2
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    • pp.135-142
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    • 2003
  • Robust extraction of 3D object's features, shape and global motion information from 2D image sequence is described. The object's 21 feature points on the pyramid type synthetic object are extracted automatically using color transform technique. The extracted features are used to recover the 3D shape and global motion of the object using stereo paraperspective camera model and sequential SVD(Singuiar Value Decomposition) factorization method. An inherent error of depth recovery due to the paraperspective camera model was removed by using the stereo image analysis. A 30 synthetic object with 21 features reflecting various position was designed and tested to show the performance of proposed algorithm by comparing the recovered shape and motion data with the measured values.

A Spelling Error Correction Model in Korean Using a Correction Dictionary and a Newspaper Corpus (교정사전과 신문기사 말뭉치를 이용한 한국어 철자 오류 교정 모델)

  • Lee, Se-Hee;Kim, Hark-Soo
    • The KIPS Transactions:PartB
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    • v.16B no.5
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    • pp.427-434
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    • 2009
  • With the rapid evolution of the Internet and mobile environments, text including spelling errors such as newly-coined words and abbreviated words are widely used. These spelling errors make it difficult to develop NLP (natural language processing) applications because they decrease the readability of texts. To resolve this problem, we propose a spelling error correction model using a spelling error correction dictionary and a newspaper corpus. The proposed model has the advantage that the cost of data construction are not high because it uses a newspaper corpus, which we can easily obtain, as a training corpus. In addition, the proposed model has an advantage that additional external modules such as a morphological analyzer and a word-spacing error correction system are not required because it uses a simple string matching method based on a correction dictionary. In the experiments with a newspaper corpus and a short message corpus collected from real mobile phones, the proposed model has been shown good performances (a miss-correction rate of 7.3%, a F1-measure of 97.3%, and a false positive rate of 1.1%) in the various evaluation measures.

A Study on Floating Collar of Dragged Gears (인망그물의 부양깃판에 관하여)

  • 장지원
    • Journal of the Korean Society of Fisheries and Ocean Technology
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    • v.22 no.1
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    • pp.1-5
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    • 1986
  • In order to improve the net-mouth height of dragged gears, the authors devised models of floatingcollars of nylon cloth instead of floats and experimented with 4 types-A type (length 65em, breadth 3em), B type (length 65em, breadth 4em), C type (length 65em, breadth 5em) and D type (length 65 em, breadth 6em) attached respectively to the front edge of square of a model net after preliminary experimentation. These various types of floating collars were experimented in a circulating water channel to evaluate the characteristics of net-mouth height and hydrodynamic resistance and the effect of the length of bridles were also examined. The results obtained were as follows: 1. In case of attaching floats, the model net-mouth height reduced from 80 em to 20 em when current velocity was increased from 0.25m/see to 1m/sec. 2. In case of attaching floating collars, the model net-mouth heights were maintained 70 em, 71 em, 80 em, 78 em in maximum and 55 em, 63 em, 69 em, 73 em in minimum respectively even the current volocity was increased from 0.25 m/see to 1 m/see. 3. The model net-mouth height was reduced to 10 em maximum according to the current velocity and types of floating collars when the bridles were shortened 3~4 mm in length. 4. Hydrodynamic resistance of D type only was increased to 700 g in maximum and those of A, B, C type were reduced to 460 g in maximum at current velocity beyond 0.5 m/ see when bridles were shortened 3-4 mm in length. 5. But the model net-mouth heights became higher in accordance with breadth of floating coliars, B type was the best for this model net in case that hydrodynamic resistance was taken into account.

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The Magnetoresistance in Iron-based Superconductors

  • Lv, B.;Xie, R.B.;Liu, S.L.;Wu, G.J.;Shao, H.M.;Wu, X.S.
    • Journal of Magnetics
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    • v.16 no.2
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    • pp.192-195
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    • 2011
  • The phase transition of vortex matter from solid to liquid was studied in iron-based superconductors. Based on the traditional vortex glass theory, we have examined the magnetoresistivity data of iron-based superconductors using our extended thermal activation model: $\rho(B,T)=\rho((T-T_g(B))/(T_c(0)-T_g(B)))^{v(z-1)}$. We predict that the magnetic field-dependent area S + $S_0$ which integrates $\rho$ with T is proportional to $B^{\beta}$, where ${\beta}$ is the vortex glass transition exponent. From our calculation, the vortex glass transition exponent is 0.33, close to the exponent of area $S_0$ + S is 0.31 in $SmO_{0.9}F_{0.1}FeAs$; the exponent of area S is 0.63, which is close to the irreversibility line exponent 2/3. Both of the results show the validity of our model. In addition, our model is shown to be effective in describing irreversibility behavior in layered superconductors.

A Convergent Investigation on the Air Flow Analysis of a Light Aircraft Propeller (경비행기 프로펠러의 공기 유동해석에 관한 융합 연구)

  • Choi, Kye-Kwang;Cho, Jae-Ung
    • Journal of the Korea Convergence Society
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    • v.11 no.12
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    • pp.131-135
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    • 2020
  • In this study, the models with three, five and ten wings of the propeller which made a light aircraft fly were performed by air flow analyses. As for the flow model A with the shape with five wings, Model A can be seen to be the most ideal flow of air. The flow of air through the number of wings, which is not too many or too few, shows the most smooth flowing form. The smaller the number of propeller blades, the smaller the flow of air. Model A is applied under pressure of up to 0.5631 MPa at the front of air flow. Also, models B and C are applied under pressures of 0.5758 MPa and 0.5589 MPa, respectively. Comparing the pressure contours for each model of flux, model B can be shown to have the highest pressure distribution. The result of this study can be used to investigate the air flow without actual testing. It also seems to be helpful in the aesthetic convergent design of light aircraft propeller.