• Title/Summary/Keyword: mixed media

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The physiological characteristics of Pleurotus ferulae Lanzi (아위버섯균의 생리적 특성)

  • Kim, Dae-Sik;Chai, Jung-Ki;Wi, Kye-Moon;Yoon, Dai-Ryoung;Oh, Deuk-Sil;Chang, Kyoung Soo;Son, Han-Gil
    • Journal of Mushroom
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    • v.2 no.2
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    • pp.88-96
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    • 2004
  • This study was executed to decide the physiological characteristics of Ferule mushroom. Four strains of Ferule mushroom were tested to select a superior strain in its mycelial growth. The pertinent substrates, temperature and pH ranges for the growth of selected strain were determined. And then, the wood rotting ability and type of the Ferule mushroom were determined. The superior strain F-2 among four strains was selected, on the basis of its vegetative mycelial growth and density on agar media. Mycelial growth of F-2 was the best on MYPA among other tested synthetic or semi-synthetic media. The temperature range for pertinent mycelial growth was about $25{\sim}34^{\circ}C$ and best at $30^{\circ}C$. The optimum pH range on MYPA was 5.0~6.0. The mycelial growth was mostly stimulated by soluble starch at cont. 1% (w/w) and secondly, maltose among several carabon sources and by mixed solution of YE(0.25%) and ME(0.25%) but not by ME alone. Cell thining and erosion of Pinus rigida wood by the mycelia of Ferule mushroom were found only on a few cell but largely at wood block test, indicating that the softwood rotting ability of Ferule mushroom mycelia was not so good. The result of polarized light microscopy appeared that cellulose of some tracheides showing the S3 layer lost brifringence was degraded by Ferule mushroom. But only part of cellulose of P. rigida wood was degraded by Ferule mushroom, because most of wood cells continued to showing briefingence. A largely degraded ray parenchyma and longitudinal parenchyma cell and partly thinning and erosion of hardwood(Quercus serrata) cell was found and it indicates that the rotting ability of Ferule mushroom mycelia on hardwood was higher than on softwood. It could be concluded that the difference in the wood rot by Ferule mushroom between the hardwood and softwood was made by the difference of chemical constitutions between them, especially in the contents and the types of lignin. Ferule mushroom was considered as white rotter as a result of bavendam test, although more research should be required.

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Effects of acid-treatment conditions on the surface properties of the RBM treated titanium implants (산-처리 조건이 RBM처리한 티타늄 임플란트의 표면 특성에 주는 영향)

  • Lee, Han-Ah;Seok, Soohwang;Lee, Sang-Hyeok;Lim, Bum-Soon
    • Korean Journal of Dental Materials
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    • v.45 no.4
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    • pp.257-274
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    • 2018
  • The purpose of this study was to evaluate the effect of acid-treatment conditions on the surface properties of the RBM (Resorbable Blast Media) treated titanium. Disk typed cp-titanium specimens were prepared and RBM treatments was performed with calcium phosphate ceramic powder. Acid solution was mixed using HCl, $H_2SO_4$ and deionized water with 4 different volume fraction. The RBM treated titanium was acid treated with different acid solutions at 3 different temperatures and for 3 different periods. After acid-treatments, samples were cleaned with 1 % Solujet solution for 30 min and deionized water for 30 min using ultrasonic cleanser, then dried in the electrical oven ($37^{\circ}C$). Weight of samples before and after acid-treatment were measured using electric balance. Surface roughness was estimated using a confocal laser scanning microscopy, crystal phase in the surface of sample was analyzed using X-ray diffractometer. Surface morphology and components were evaluated using Scanning Electron Microscope (SEM) with Energy Dispersive X-ray spectroscopy (EDX) and X-ray Photoemission Spectroscopy (XPS). Values of the weight changes and surface roughness were statistically analyzed using Tukey-multiple comparison test (p=0.05). Weight change after acid treatments were significantly increased with increasing the concentration of $H_2SO_4$ and temperature of acid-solution. Acid-treatment conditions (concentration of $H_2SO_4$, temperature and time) did not produce consistent effects on the surface roughness, it showed the scattered results. From XRD analysis, formation of titanium hydrides in the titanium surface were observed in all specimens treated with acid-solutions. From XPS analysis, thin titanium oxide layer in the acid-treated specimens could be evaluated. Acid solution with $90^{\circ}C$ showed the strong effect on the titanium surface, it should be treated with caution to avoid the over-etching process.

Influence of Incorporation Rates of Sulfur on Reduction of the Bicarbonate Injury in Hydroponic Cultivation of 'Ssanta' Strawberry (유황 처리수준이 '싼타' 딸기의 중탄산 피해 경감에 미치는 영향)

  • Lee, Hee Su;Kim, Yun-Seob;Park, In Sook;Cheung, Jong Do;Choi, Jong Myung
    • Journal of Bio-Environment Control
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    • v.27 no.2
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    • pp.140-146
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    • 2018
  • This research was conducted to determine the influence of incorporation levels of sulfur into a coir dust+pine bark medium (1:1, v/v) on the changes in the bicarbonate ($HCO_3{^-}$) concentrations and pH of soil solution, crop growth, and nutrient uptake of 'Ssanta' strawberry. In the preparing of the mixed medium, sulfur powder was added with the rate of 0 (control), 0.23, 0.45, 0.90, and $1.80g{\cdot}L^{-1}$ and Hoagland nutrient solution containing $240mg{\cdot}L^{-1}$ $HCO_3{^-}$ was supplied during the crop cultivation. The growth measurements and tissue analysis for the determination of nutrient contents were carried out 140 days after solution application and the soil solution analysis was performed every two weeks. As the level of sulfur was elevated, the overall growth of mother plants became better showing that the growth indexes except chlorophyll contents were the lowest in control treatment but the statistical differences were not found among the three treatments of $0.45g{\cdot}L^{-1}$ or higher sulfur. The higher the levels of sulfur incorporation, the higher the occurrence of runners and the growth of daughter plants. The length of the runners and the number of daughter plants occurred per mother plants were higher in the treatments of 0.90 and $1.80g{\cdot}L^{-1}$ than the three treatments of 0, 0.23, and $0.45g{\cdot}L^{-1}$, but the statistical differences were not observed between the 0.90 and $1.80g{\cdot}L^{-1}$ treatments. The rose of pH and $HCO_3{^-}$ concentrations in soil solution of root media continued all the cropping period, but those decreased slightly in the treatments of $0.90g{\cdot}L^{-1}$ or higher. The soil solution concentrations of $K^+$ and $PO_4{^3-}$ in the treatments of 0.90 and $1.80g{\cdot}L^{-1}$ was lower than those in other treatments and the statistical differences in the $Ca^{2+}$ and $Mg^{2+}$ concentrations were not observed among all treatments. The nutrient contents in tissue analyzed in this experiment were the lowest in the control treatments and those increased as incorporation rates of sulfur were elevated. Above results indicated that when 'Ssanta' strawberry is grown hydroponically and the root medium is coir dust and the pine bark (5:5, v/v) mix, the sulfur incorporation rate as pre-planting fertilizer has to be higher than $0.9g{\cdot}L^{-1}$ root medium to reduce the $HCO_3{^-}$ injury.

Changes in Crop Growth and Nutrient Concentrations of Tissue and Soil Solution in Raising of Hot Pepper Plug Seedlings as Influenced by Various Pre-planting Nitrogen Levels Incorporated into a Inert Medium (상토 조제과정에서 혼합된 질소 시비 수준 차이가 고추 플러그 묘 생장과 상토 및 식물체 무기염 농도 변화에 미치는 영향)

  • Oh, Sang Se;Kim, Yun-Seob;Park, Myong Sun;Kim, Hyun Cheul;Choi, Jong Myung
    • Journal of Bio-Environment Control
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    • v.27 no.2
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    • pp.173-179
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    • 2018
  • Investigation of the optimum levels of pre-plant nitrogen for raising of hot pepper (cv. Nokkwang) plug seedlings was the objective of this research. To achieve this, the pre-plant nitrogen levels were varied to 0, 100, 250, 500, 750, 1,000, and $1,500mg{\cdot}L^{-1}$ and the other essential nutrients were controlled to equal concentrations in all treatments. All the fertilizers were added during the formulation of the mixed medium of coir dust, peatmoss, and perlite with the ratio of 35, 35, and 30% (v/v/v). The root medium containing pre-plant fertilizer was packed into 50-cell plug trays and seeds were sown. The measurement of pH and EC in every week, soil solution analysis for nutrients in week 0, 3, and 7 and growth measurements as well as tissue analysis for nutrient contents in week 7 were conducted. The pHs measured before seed sowing did not show significant differences, but the differences among treatments became significant as seedlings grow bigger. The soil solution ECs were significantly different among treatments in week 0 and these differences were diminished by degrees after week 3, resulting in no significant differences among treatments in week 7. The trends in changes of $NH_4-N$, $NO_3-N$, and other the macro-element concentrations in soil solution of root media were similar to those of ECs. The treatments of 500 and $750mg{\cdot}L^{-1}$ N were more effective than other treatments on seedling growth. The seedling growths in the treatments containing higher N than $1,000mg{\cdot}L^{-1}$ and control were severely suppressed. The elevated pre-plant N concentrations in the root medium resulted in the increase of tissue N contents. The treatments of 500 and $750mg{\cdot}L^{-1}$ N shown the highest seedling growths had 5.13% and 5.31%, respectively, in tissue N contents based on the dry weight of above ground tissue at week 7. The results of this study indicated that the optimum level of pre-plant N is 500 to $750mg{\cdot}L^{-1}$ for the raising of hot pepper plug seedlings.

Characteristics of Culture of Oyster Mushroom (Pleurotus ostreatus) on Addition Rate of Acer tegmentosum (산겨릅나무(Acer tegmentosum) 톱밥의 첨가량에 따른 느타리버섯의 재배적 특성)

  • Lee, Chan-Jung;Jhune, Chang-Sung;Cheong, Jong-Chun;Moon, Ji-Won;Kong, Won-Sik;Suh, Jang-Sun;Park, Gi-Chun;Shin, Yu-Su
    • The Korean Journal of Mycology
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    • v.41 no.1
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    • pp.21-27
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    • 2013
  • This study was carried out to investigated optimum mixing ratio of Korean natural Acer tegmentosum for production of functional oyster mushroom. Total nitrogen and carbon sources of Acer tegmentosum were 0.2% and 44.4%, respectively and C/N ratio was 234. Total nitrogen source and pH of substrate mixed with Acer tegmentosum were 2.7~2.9 and 4.8~5.0, respectively. The contents of $P_2O_5$, $K_2O$ and MgO at Acer tegmentosum media were higher than that of the control. Mycerial growth was the fastest at Acer tegmentosum 10%, and slower by increase of Acer tegmentosum substrate. Yields of fruiting body was the highest to 159 g/850 mL at 10% of Acer tegmentosum and dimeter and thick of pileus were the highest, too. The L value of pileus and stipes were decreased by increase of Acer tegmentosum substrate, but there was no significant difference in the a-value and the b-value. The contents of $P_2O_5$ and $K_2O$ of fruiting body were increased at Acer tegmentosum substrate, but there was no significant difference in contents of CaO, MgO and $Na_2O$.

Quantification of endothelin-1 in human umbilical venous endothelial cell culture supernatants of small for gestational age and preeclampsia neonates (부당 경량아 및 전자간증 산모의 신생아 제대혈관내피세포의 endothelin-1 발현 비교)

  • Cho, Won Kyoung;Kim, So Young;Chun, Chung Sik
    • Clinical and Experimental Pediatrics
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    • v.50 no.12
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    • pp.1194-1199
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    • 2007
  • Purpose : It was generally accepted now a days that the pathogenesis of preeclampsia, small for gestational age (SGA), intrauterine growth retardation and fetal origin of adult diseases were related with a endothelial cell dysfunction. The purpose of this study was to know the relation of such diseases by assessing the level of endothelin-1. Methods : SGA babies, newborns of preeclampsia and normal control mother were included in this study. Isolated endothelial cells were centrifugated and mixed with media in $37^{\circ}C$, 5% $CO_2$ to obtain confluent monolayer of cultured human umbilical venous endothelial cell (HUVEC). Endothelin-1 levels were determined by Endothelin-1 colorimetric (EIA) Kits. We examined the endothelin-1 level in the HUVEC supernatants from SGA baby, and newborns from preeclampsia as well as normal mother. Also, we compared the endothelin-1 level of cultured normal HUVEC incubated with serum from cord blood of SGA, babies of preeclampsia or normal control mother. Results : The endothelin-1 levels in cultured HUVEC supernatants of three groups showed no significant difference but the endothelin-1 levels of cultured normal HUVEC incubated with serum from preeclampsia mother or SGA mother was significantly higher than those from newborns of control mothers (P<0.05). Conclusion : These findings suggest that there may be the factor which affect the endothelin-1 level in serum of cord blood from SGA and preeclampsia.

The Effect of Freeze and Thaw for the Stabilized Soil Bottom Liners in the Landfill (폐기물 매립지 바닥층의 고화토 포설시 동결/융해 현상에 관한 연구)

  • Lee, Song;Lee, Jai-Young;Kim, Heung-Suck
    • Journal of the Korean Geotechnical Society
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    • v.16 no.1
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    • pp.179-189
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    • 2000
  • The purpose of this research is to complement the existing researches on landfill bottom liners behavior during the periods of freeze and thaw. Landfill-related researches have been typically focused on small-scale soil samples that are often compacted under conditions different from those used in the field. Although these tests have been invaluable in clarifying the problem of freeze and thaw, extending the results of such experimental studies to prototype landfills are questionable. In this investigation, the author utilized a large scale laboratory simulation allowing inclusion of the field depth of the cover systems, layered soil profiles, rainfall simulation, a cold climate and boundary conditions similar to those encountered in the landfill. The soil materials were stabilized soils (mixed clays, cements, and minerals) instead of clays. The bottom liners are made up of drainage layer (30 cm), stabilized layer (75 cm), and leach collection layer (60 cm). The stabilized layers are made up of supporting layer (45 cm) and low permeable layer (30 cm) - consisting of $P_A\; and\; P_B$ layer. As a results, depths of penetration increased by about 2~5 more centimeters at rainfall simulated designs than those at no rainfall simulated designs (that is design 3, design 5 and design 7) - it increased by about 20mm/day in the bottom liners and frost heaves also increased it by a few millimeters. Also, a few cracks appeared partly. According to these results, we can surmise that the compacted stabilized soil is more reliable than the compacted clay liners for construction of the landfill liners.

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A research on EEG coherence variation by relaxation (이완에 따른 EEG 코히런스 변화에 대한 연구)

  • Kim, Jong-Hwa;Whang, Min-Cheol;Woo, Jin-Cheol;Kim, Chi-Joong;Kim, Young-Woo;Kim, Ji-Hye;Kim, Dong-Keun
    • Science of Emotion and Sensibility
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    • v.13 no.1
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    • pp.121-128
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    • 2010
  • This study is to analyze change of connectivity between brain positions caused by relaxation through EEG coherence. EEG spectrum analysis method has been used to analyze brain activity when relaxation was experienced. However, the spectrum analysis method has a limit that could not observe interactive reaction between brain-functional positions. Therefore, coherence between positions was analyzed to observe connectivity between the measurement positions in this study. Through the method, the reaction of the central nervous system caused by the emotion change was observed. Twenty-four undergraduates of both genders(12 males and 12 females) were asked to close their eyes and listen to the sound. During experiment, EEG was measured at eight positions. The eight positions were F3, F4, T3, T4, P3, P4, O1, and O2 in accordance with International 10-20 system. The sounds with white noise and without were used for relaxation experience. Subjective emotion was measured to verify whether or not they felt relaxation. Subjective emotion of participants were analyzed by ANOVA method(Analysis of Variance). In the result, it was proved that relaxation was subjectively evoked when participants heard sound. Accordingly, it was proved that relaxation could be enhanced by the mixed white noise. EEG coherence between the measurement positions was analyzed. T-test was performed to find its significant difference between relaxation and not-relaxation. In the results of EEG coherence, connectivity with occipital lobes has been increased with relaxation, and connectivity with parietal lobes has been increased with non-relaxed state. Therefore, brain connectivity has shown different pattern between relaxed emotion and non-relaxed emotion.

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Recirculating Integrated System for the Treatment of Authentic Integrated-textile-dyeing Wastewater from Dyeing Industrial Complex (염색산업단지 종합폐수처리용 재순환 통합시스템)

  • Lee, Eun Ju;Lim, Kwang-Hee
    • Korean Chemical Engineering Research
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    • v.55 no.6
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    • pp.837-845
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    • 2017
  • A recirculating integrated system composed of a fluidized biofilter filled with waste-tire crumb media fixed with return sludge from wastewater treatment facility of D dyeing industrial center, and a UV/photocatalytic reactor packed with calcined $TiO_2$ coated-glass beads as photocatalyst-support, was constructed and was run to treat authentic textile-dyeing wastewater from D-dyeing industrial center, which was mixed with an alkaline polyester-weight-reducing wastewater and a wastewater from sizing process. As a result, its total removal efficiency(RE(tot)) of $COD_{cr}$ and colors were ca. 81% and 55%, respectively. The synergy effect of the recirculating integrated system to enhance total removal efficiency(RE(tot)) of $COD_{cr}$ and colors were evaluated at most ca. 7% and 3%, respectively. The fluidized biofilter and the UV/photocatalytic reactor were responsible for ca. 94% and 6% of the total $COD_{cr}$ removal efficiency, respectively, and were also responsible for ca. 86% and 14% of the total color-removal efficiency, respectively. Thus, the degree of the UV/photocatalytic reactor-unit process's contribution to RE(tot) of color, was about 2.4 times of that to RE(tot) of $COD_{cr}$. Therefore, the UV/photocatalytic reactor facilitated the more effective elimination of colors by breaking down the chemical bonds oriented from colors of dyes such as azo-bond, than $COD_{cr}$. In addition, the effect of the removal efficiency of each unit process(i.e., the fluidized biofilter or the UV/photocatalytic reactor) of the recirculating integrated system on RE(tot) of $COD_{cr}$ and colors, was analysed by establishing its model equation with an analytic correlation.

Removal of Soluble Mn(II) using Multifunctional Sand Coated with both Fe- and Mn-oxides (철과 망간이 동시에 코팅된 다기능성 모래를 이용한 용존 Mn(II) 제거)

  • Lim, Jae-Woo;Chang, Yoon-Young;Yang, Jae-Kyu
    • Journal of Korean Society of Environmental Engineers
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    • v.32 no.2
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    • pp.193-200
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    • 2010
  • This study evaluated treatability of soluble Mn(II) using multifunctional sand media simultaneously coated with iron and manganese. In the preparation of IMCS(Iron and Manganese Coated Sand), 0.05 M Mn(II) solution and Fe(III) solution was mixed with sand at pH 7. The mineral type of IMCS was identified as the mixture of ${\gamma}-MnO_2$, goethite and magnetite($F_{e3}O_4$). The contents of Mn and Fe coated onto sand were 826 and 1676 mg/kg, respectively. The $pH_{pzc}$ of IMCS was measured as 6.40. The removal of soluble Mn(II) using IMCS and oxidants such as NaOCl and $KMnO_4$ was investigated with variation of the solution pH, reaction time and Mn(II) concentration in a batch test. The removal of Mn(II) on IMCS was 34% at pH 7.4 and the removals of Mn(II) on IMCS in the presence of NaOCl(13.6 mg/L) at pH 7 and $KMnO_4$(4.8 mg/L) at pH 7.6 were 96% and 89%, respectively. The removal of Mn(II) using IMCS and oxidants followed a typical cationic type, showing a gradual increase of removal as the solution pH increased. The removal of Mn(II) was rapid in the first 6 hrs and then a constant removal was observed. The maximum removed amount of Mn(II) on IMCS-alone and IMCS in the presence of oxidants such as NaOCl(13.6 mg/L) and $KMnO_4$(4.8mg/L) were 833.3, 1428.6 and 1666.7 mg/kg, respectively. Mn(II) removal onto the IMCS in the presence of oxidants was well described by second-order reaction and Langmuir isotherm expression.