• 제목/요약/키워드: microbial growth

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중·소하천에서 부착미생물군집의 성장에 따른 불소 제거 효과 평가 (Evaluation of Fluoride Removal Effect with Growth of Attached Microbial Community in Middle and Small Stream)

  • 김태경;류서영;박윤아;이종준;주광진;장광현;오종민
    • 환경영향평가
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    • 제27권6호
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    • pp.595-603
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    • 2018
  • 본 연구는 경기도 중소하천에서 하상의 부착미생물군집의 시간에 따른 생장 변화와 부착미생물군집의 성장에 따른 불소의 제거 효율을 파악하기 위해 수행하였다. 방류수가 유입되는 지점인 P1과 그 곳에서 약 2 km 유하한 하류 지점인 P2에서 12주 동안의 현장 모니터링을 실시하고, 조사 지점의 수질과 부착미생물군집의 생체량 분석을 진행하였다. 부착미생물군집의 성장량은 7차 조사 시점까지 증가하다 이후 탈리현상이 발생하는 것으로 관찰되었으며, 부착미생물군집의 성장에 영향을 미치는 요인으로는 유량, 유속, 영양물질(질소, 인) 등으로 조사되었다. 한편, 부착미생물군집 체내의 불소 함유량 또한 7차 조사 시기까지 증가하다 8차 시점부터 감소하는 경향을 보였다. 이는 부착미생물군집의 탈리현상에 의해 불소의 함유량 또한 감소하였음을 사사한다. 이를 통해 하천 관리의 방안으로서 부착미생물군집의 활용법에 대한 평가 및 관리에 기여할 수 있을 것으로 기대된다. 부착미생물군집을 활용한 기법의 적용 시에는 부착미생물군집의 성장에 영향을 미칠 수 있는 요인에 대한 기초 조사 및 탈리 시점에 따른 부착판 교체 방안 등이 포함되어야할 것으로 사료된다.

Relationship of Specific Microbial Growth and TBARS Value in Radiation-Sterilized Raw Ground Pork

  • Kim, Jae-Kyung;Jo, Cheo-Run;Kim, Hyun-Joo;Lee, Kyong-Haeng;Kim, Yeung-Ji;Byun, Myung-Woo
    • Preventive Nutrition and Food Science
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    • 제9권4호
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    • pp.312-316
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    • 2004
  • Sterilized raw ground pork was inoculated with Pseudomonas aeruginosa (PA) and Lactobacillus casei (LC) to investigate the relationship between microbial growth and 2-thiobarbituric acid reactive substances (TBARS) values. The analyses including microbial growth, pH, and TBARS values were performed during 3 weeks of storage at room temperature $(20^{\circ}C)$. The radiation-sterilized control sample did not show any microbial growth, but the samples inoculated at different levels (diluted twice vs non-diluted) exhibited differences until 1 week. However, the difference disappeared at weeks 2 and 3. The pH of raw ground pork inoculated with PA increased, but that of LC decreased. The pH of non-inoculated samples increased slightly after storage. The TBARS values in non-inoculated and LC inoculated with pork increased, but TBARS remained unchanged in samples inoculated with PA after 1 week. Results indicated that the microbial growth level and strains can influence the TBARS value of raw ground pork. Thus, it is important to use samples exposed to the same microbial conditions to compare the oxidation of lipids in meat samples.

미생물제제의 토양처리가 인삼생육 및 수량에 미치는 영향 (Effects of Soil Treatments with Microbial Materials on Growth and Yield of Korean Ginseng)

  • 최재을;이은정;김영창;유성준
    • 농업과학연구
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    • 제27권1호
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    • pp.1-4
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    • 2000
  • 본 시험은 미생물제제의 토양처리가 고려인삼의 생육 및 수량에 미치는 영향을 조사하기 위하여 실시하였다. 미생물제제의 토양처리는 고려인삼의 생육 및 수량증대에 크게 작용하였다. 미생물제의 토양처리로 5개월 후에 인삼의 생체중이 32-68% 증가하였다. 미생물제제에 의한 생육촉진 및 수량증대효과는 근권의 미생물상의 변화에 기인된 것으로 추정된다.

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Analysis of Temperature Effects on Microbial Growth Parameters and Estimation of Food Shelf Life with Confidence Band

  • Park, Jin-Pyo;Lee, Dong-Sun
    • Preventive Nutrition and Food Science
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    • 제13권2호
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    • pp.104-111
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    • 2008
  • As a way to account for the variability of the primary model parameters in the secondary modeling of microbial growth, three different regression approaches were compared in determining the confidence interval of the temperature-dependent primary model parameters and the estimated microbial growth during storage: bootstrapped regression with all the individual primary model parameter values; bootstrapped regression with average values at each temperature; and simple regression with regression lines of 2.5% and 97.5% percentile values. Temperature dependences of converted parameters (log $q_o$, ${\mu}_{max}^{1/2}$, log $N_{max}$) of hypothetical initial physiological state, maximum specific growth rate, and maximum cell density in Baranyi's model were subjected to the regression by quadratic, linear, and linear function, respectively. With an advantage of extracting the primary model parameters instantaneously at any temperature by using mathematical functions, regression lines of 2.5% and 97.5% percentile values were capable of accounting for variation in experimental data of microbial growth under constant and fluctuating temperature conditions.

미생물제제 처리가 토양 미생물상의 변화 및 배추의 생육에 미치는 영향 (Effect of Microbial Product on Microorganisms in Soil and Growth of Chinese Cabbage)

  • 석운영;오주성;김도훈;정원복;정순재
    • 한국유기농업학회지
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    • 제12권4호
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    • pp.399-409
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    • 2004
  • This study was conducted to investigate the effect of different concentrations of microbial products on growth of chinese cabbage and microorganisms in soil. Two different levels of microbial products, such as 50 times and 100 times diluted solutions of chitosan, wood vinegar and EM activity liquid, were treated for foliar application. the results were summarized as follows : Among foliar applications of microbial products, 100 times diluted solution of chitosan was effective on growth of chinese cabbage comparing to other levels of dilutions and untreated control plot. The number of microorganism in the soil tended to increase under the treatment of microbial products compared to control plot. Especially, the numbers of the bacteria and actinomycetes were estimated $73.67{\times}10^3$ CFU/g and $34.00{\times}10^3$ CFU/g, respectively, under the treatment of 100 times diluted solution of chitosan.

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홍삼박을 활용한 토양미생물제제가 수박의 생육에 미치는 영향 -현장연구를 중심으로- (Effects of Soil Microbial Agent with Red Ginseng Marc on Growth of Watermelon -A Field Study-)

  • 류효승;이종화;김창만;최인학
    • 한국환경과학회지
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    • 제24권12호
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    • pp.1705-1710
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    • 2015
  • The aim of the present study was to determine the effects of soil microbial agent with red ginseng marc on growth of watermelon during 5 months. The three treatments were distributed in a completely randomized design with four replicates per plot. After 1 week in planting dates, the growth of watermelon (full length, stem thichness, leaf length and lead width) showed no significant difference in all treatments. During elongation stage (20 days), soil microbial agent with red ginseng marc was increased by 5% in leaf thickness (May 23) and 7~14% in leaf length (May 16 and 23) when compared to other treatments. For changes in fruit bearing thickness, there were no differences among treatments. Characteristics of watermelon in harvest season have an effect on harvest and length, stalk length, naval length, weight, sugar content and yield, except for harvest and width. In particular, yields increased with treatments with two soil microbial agent (7~12%), indicating that soil microbial agent with red ginseng marc showed higher yield than the other treatments. In conclusion, red ginseng marc-treated soil microbial agents have a positive effect on the harvest season of watermelon and can provide useful information for the selection of the functional microbial properties and the registration of microbial fertilizer.

Effects of Chemical Compounds on Vase Life and Microbial Growth of Cut Calla Flowers

  • Lee, Seon-Ha;Kim, Jung-Ho
    • Plant Resources
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    • 제3권1호
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    • pp.59-65
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    • 2000
  • The opening process of cut calla flower was faster at 30 t than at lower temperatures as it could be expected from its tropical origin. Gibberellin enhanced the flower opening, however, it also speeded up senescent. Silver thiosulfate was effective in prolonging the vase life of the cut calla flower. Silver thiosulfate reduced ethylene generation by the flower and inhibited microbial growth in the flower stalk. Reduction in ethylene generation and inhibition of microbial growth is thought to be responsible for the extension of the vase life of cut calla flowers by silver thiosulfate.

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미생물제 Mity-Gro$^{TM}$의 작물재배효과에 관한 연구 (Effects of Plant Cultivation with Microbial Inoculant, Mity-Gro$^{TM}$)

  • 윤세영
    • 한국유기농업학회지
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    • 제8권1호
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    • pp.139-145
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    • 1999
  • The experiment was conducted to determine the effects of Plant growth and the microbial distribution in soils treated with microbial products, Mity-GroTM. The results from this experiment were as follows ; 1. Plant height of Tomato and Pepper were significantly increased at 30 days after planted in soils treated with Mity-GroTM. Therefore, treatment of microbial products, Mity-GroTM, was considered to contribute the plant growth at early stage. 2. Microbial distribution in soils treated with Mity-GroTM was significantly changed at specific mcirobial population. However, The ratio of bacteria/actinomycetes in the plot treated with Mity-GroTM was significantly enhanced.

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Two Bacterial Entophytes Eliciting Both Plant Growth Promotion and Plant Defense on Pepper (Capsicum annuum L.)

  • Kang, Seung-Hoon;Cho, Hyun-Soo;Cheong, Hoon;Ryu Choong-Min;Kim, Ji-Hyun;Park, Seung-Hwan
    • Journal of Microbiology and Biotechnology
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    • 제17권1호
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    • pp.96-103
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    • 2007
  • Plant growth-promoting rhizobacteria (PGPR) have the potential to be used as microbial inoculants to reduce disease incidence and severity and to increase crop yield. Some of the PGPR have been reported to be able to enter plant tissues and establish endophytic populations. Here, we demonstrated an approach to screen bacterial endophytes that have the capacity to promote the growth of pepper seedlings and protect pepper plants against a bacterial pathogen. Initially, out of 150 bacterial isolates collected from healthy stems of peppers cultivated in the Chungcheong and Gyeongsang provinces of Korea, 23 putative endophytic isolates that were considered to be predominating and representative of each pepper sample were selected. By phenotypic characterization and partial 16S rDNA sequence analysis, the isolates were identified as species of Ochrobacterium, Pantoea, Pseudomonas, Sphingomonas, Janthinobacterium, Ralstonia, Arthrobacter, Clavibacter, Sporosarcina, Acidovorax, and Brevundimonas. Among them, two isolates, PS4 and PS27, were selected because they showed consistent colonizing capacity in pepper stems at the levels of $10^6-10^7CFU/g$ tissue, and were found to be most closely related to Pseudomonas rhodesiae and Pantoea ananatis, respectively, by additional analyses of their entire 16S rDNA sequences. Drenching application of the two strains on the pepper seedlings promoted significant growth of peppers, enhancing their root fresh weight by 73.9% and 41.5%, respectively. The two strains also elicited induced systemic resistance of plants against Xanthomonas axonopodis pv. vesicatoria.

Humic Substances Act as Electron Acceptor and Redox Mediator for Microbial Dissimilatory Azoreduction by Shewanella decolorationis S12

  • Hong, Yi-Guo;Guo, Jun;Xu, Zhi-Cheng;Xu, Mei-Ying;Sun, Guo-Ping
    • Journal of Microbiology and Biotechnology
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    • 제17권3호
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    • pp.428-437
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    • 2007
  • The potential for humic substances to serve as terminal electron acceptors in microbial respiration and the effects of humic substances on microbial azoreduction were investigated. The dissimilatory azoreducing microorganism Shewanella decolorationis S12 was able to conserve energy to support growth from electron transport to humics coupled to the oxidation of various organic substances or $H_2$. Batch experiments suggested that when the concentration of anthraquinone-2-sulfonate (AQS), a humics analog, was lower than 3 mmol/l, azoreduction of strain S12 was accelerated under anaerobic condition. However, there was obvious inhibition to azoreduction when the concentration of the AQS was higher than 5 mmol/l. Another humics analog, anthraquinone-2-sulfonate (AQDS), could still prominently accelerate azoreduction, even when the concentration was up to 12 mmol/l, but the rate of acceleration gradually decreased with the increasing concentration of the AQDS. Toxic experiments revealed that AQS can inhibit growth of strain S12 if the concentration past a critical one, but AQDS had no effect on the metabolism and growth of strain S12 although the concentration was up to 20 mmol/l. These results demonstrated that a low concentration of humic substances not only could serve as the terminal electron acceptors for conserving energy for growth, but also act as redox mediator shuttling electrons for the anaerobic azoreduction by S. decolorationis S12. However, a high concentration of humic substances could inhibit the bacterial azoreduction, resulting on the one hand from the toxic effect on cell metabolism and growth, and on the other hand from competion with azo dyes for electrons as electron acceptor.