• 한국수산과학회지
• /
• 제40권1호
• /
• pp.8-15
• /
• 2007

This study selected the optimal microalgal species for mass culture of a brackish water copepod Paracyclopina nana. Fifteen microalgal species were tested to examine nauplius production and the survival and maturation of brood females. Total and daily nauplius production were highest in P. nana fed Tetraselmis suecica followed Isochrysis galbana, Dunaliella tertiolecta, and Phaeodactylum tricornutum. With a monospecific microalgal diet, the total density was highest with P. nana fed I. galbana, at 63.3 inds./mL. With a mixed microalgal diet, the total density of P. nana fed T. suecica+I. galbana was higher than that fed other mixed diets, although there was no difference between a monospecific diet of I. galbana and a mixed diet of T. suecica+I. galbana. Examining the fatty acid composition of P. nana, the eicosapentaenoic acid (EPA) was highest in P. nana fed T. suecica at 5.4% while the docosahexaenoic acid (DHA) content was highest I. galbana diet at 31.9%. Although no DHA was detected in T. suecica, P. nana fed this microalgal species had a high DHA composition of 24.3%. We suggest that the optimal microalgal species for the mass culture of P. nana is T. suecica which is easy to culture on masse and has a high linolenic acid content.

• Journal of Biosystems Engineering
• /
• 제24권1호
• /
• pp.9-18
• /
• 1999

This study was performed to investigate the optimum microalgal culture conditions using flask culture and to find the feasibility of using the flue gas of the rice husk incinerator for cultivating the microalgae. The optimum initial pH of media was 4.5 for the microalgae culture, and the intermittently illuminated culture was more effective than the continuous illuminated culture. Thus, the balance between photosynthesis and formative metabolism must be considered thoroughly to cultivate microalgal cells. The optimum CO2 concentrations were in the range of 7 to 10%, and the optimum temperature was about 35$^{\circ}C$ in both the daytime and the nighttime for the culture. When flue gas of the rice husk incinerator was applied to the microalgae culture using stirred photobioreactor, the dry cell weight was 0.026 g dry biomass/hr$.$l. The results obtained in experiments indicated that the flue gas was effective for microalgae culture without any limitations.

• Fisheries and Aquatic Sciences
• /
• 제14권4호
• /
• pp.339-345
• /
• 2011

Although culture techniques for the abalone Haliotis discus hannai are well known, mass culture of the benthic microalgae that are essential live food for the abalone larvae is still not practiced. This study was conducted to identify the microalgal species suitable for the growth of early larvae of H. discus hannai. The growth and attachment rates of 31 microalgal species were examined. Acrylic plates were used as the substrate. Among the 31 microalgal species, nine showing high growth and attachment rates were selected and tested for their dietary values via factors including settlement, metamorphosis, and survival rates of abalone larvae. Tetraselmis hazeni and Rhaphoneis sp. induced the highest settlement rate (65-69%) in abalone larvae. The metamorphosis rate was highest (57%) in larvae fed Rhaphoneis sp. and was also significantly higher in larvae fed Oscillatoria splendida (29%) and T. hazeni (22%) than in those fed other species. The highest survival rate of the larvae during the 15 days after metamorphosis was 67% in those fed Rhaphoneis sp., followed by T. hazeni (42%) and O. splendida (35%). In conclusion, Rhaphoneis sp. is the most suitable diatom for use as a live food for the culture of early larvae of H. discus hannai. In addition, T. hazeni and O. splendida are also potential species to be further developed and utilized in larval culture.

• ALGAE
• /
• 제17권2호
• /
• pp.117-125
• /
• 2002

Five microalgal species isolated from the Jeju coast and four microalgal stock strains in hatchery were cultured in order to investigate their adapation to extreme changes in environmental factors such as salinity, water temperatue, adn nutrients. In case of salinity variation, Nitzschia sp. of Bacillariophyceae, Isochrysis galbana of Haptophyceae and Tetraselmis gracilis of Prasinophyceae showed optimum growth at the low salinity of 20 and 25 psu. Amphora coffeaeformis and Chetoceros simplex of Bacillariophyceae, and Pavlova lutheri of Haptophyceae adapted well at the relatively high salinities of 30 and 35 psu. However Phaeodactylum tricornutum of Bacillariophyceae and Chlorella sp. of Chlorophyceae showed euryhaline property In case of water temperature variation, most of all the species studied wer inhibited at 10℃. C. simplex, Nitzschia sp., p. tricornutum, Chlorella sp. and T. gracilis grew well at above 20℃. A. coffeaeformis, I. galbana and P. lutheri adapted also at the high temperature of 30℃. Each microalgal strain showed different growth rates and its maximum biomass. Generally microalgal populations from the Jeju coast grow well in relatively high salinity and high water temperature. Their growth were inhibited at low water temperature, but not likely affected at low salinity. This study indicates that the microalgal populations could not be affected by abnormally low salinity phenomena, which have happened occasionally around the west Jeju coast in summer and have led macrobenthic animals to mass mortality.

• ALGAE
• /
• 제22권3호
• /
• pp.229-234
• /
• 2007

This study was to determine the extent of bacteria contamination and resistance to various antibiotics used commonly in microalgal culture. Seven different dose levels of chloramphenicol, dihydrostreptomycin sulphate, neomycin, penicillin G, streptomycin sulphate, penicillin G + streptomycin sulphate, and penicillin G + streptomycin sulphate + chloramphenicol were added to each culture of microalgae. The lethal effects on microalgae and bacteria were the highest in chloramphenicol and the lowest in penicillin G. The axenic culture of bacillariophyceae and dinophyceae was more difficult than that of chlorophyceae and haptophyceae because of their complicate external morphology. The efficient antibiotics and their concentrations for axenic cultures varied with microalgal species. The optimum quantity for antibiotic treatments were 2,000 ppm of dihydrostreptomycin for Chlorella ellipsoidea, neomycin 500 ppm of Isochrysis galbana and Heterosigma ahashiwo, hloramphenicol 500 ppm of Cyclotella didymus, and dihydrostreptomycin sulphate and neomycin 6,000 ppm of Thalassiosira allenii.

• 한국재료학회지
• /
• 제31권7호
• /
• pp.386-391
• /
• 2021

The formation of CaCO₃ in microalgal culture is investigated and applied for effective separation of microalgae. The presence of several cationic ions in the culture medium mediates the formation of 3 types of mineral precipitates depending on the concentration of mineral precursors, Ca²⁺ and CO₃^2-, amorphous nano-flakes, rhombohedral calcites, and spherical vaterites. While amorphous phased precipitates are formed for all concentrations of mineral precursor, only calcites are formed for 30 mM solutions of mineral precursor, and mixtures of calcites and vaterites are formed for 50 and 100 mM solutions of mineral precursor. The harvesting efficiency is also dependent on the concentration of the mineral precursor: from 90 % for 10 mM to 99 % for 100 mM after 60 mins' of gravitational sedimentation. The formation of nano-flakes on the surface of microalgal cells induces the flocculation of microalgae by breaking the stable dispersion. The negatively charged surface of the microalgal cell is compatible not only with nano-flake attachment but also with the growth of calcitic crystals in which microalgal cells are embedded.

• Journal of Microbiology and Biotechnology
• /
• 제28권4호
• /
• pp.630-637
• /
• 2018

The high rate algal ponds (HRAP) powered and mixed by a paddlewheel have been widely used for over 50 years to culture microalgae for the production of various products. Since light incidence is limited to the surface, water depth can affect microalgal growth in HRAP. To investigate the effect of water depth on microalgal growth, a mixed microalgal culture constituting three major strains of microalgae including Chlorella sp., Scenedesmus sp., and Stigeoclonium sp. (CSS), was grown at different water depths (20, 30, and 40 cm) in the HRAP, respectively. The HRAP with 20cm of water depth had about 38% higher biomass productivity per unit area ($6.16{\pm}0.33g{\cdot}m^{-2}{\cdot}d^{-1}$) and required lower nutrients and energy consumption than the other water depths. Specifically, the algal biomass of HRAP under 20cm of water depth had higher settleability through larger floc size (83.6% settleability within 5 min). These results indicate that water depth can affect the harvesting process as well as cultivation of microalgae. Therefore, we conclude that water depth is an important parameter in HRAP design for mass cultivation of microalgae.

• 한국수산과학회지
• /
• 제25권3호
• /
• pp.205-218
• /
• 1992

미소조류의 응용연구 추세를 1970년을 기준으로 양분하여 조사하였다 전반기(1970년 이전)에는 배양한 미소조류 세포체 자체를 이용하기 위한 연구가 많다. 이 때에는 유용생물의 먹이, 식용단백질, 생물비료, 유기물 폐수처리 등의 분야를 주된 연구 대상으로 삼았다. 1970년 이후(후반기)에는 미소조류 세포체의 대량생산 방식을 탈피하여, 미소조류의 세포대사 특성물질 중 유용성분을 생산하기 위한 연구가 급격히 증가하고 있다. 즉, 비타민, 아미노산, 베타 카로틴, phycofluor, 약제성분, 생물활성물질, 수소가스 및 중수소 화학물질 등 단가가 매우 높은 천연순물질의 생산을 목표로 하여 연구력을 집중하는 추세이다. 국내의 관련 연구분야는 아직 초기단계에 있다고 판단된다. 미소조류 응용분야의 미래가능성으로 보아, 국내의 관련연구 수준을 시급히 향상시킬 필요가 있다. 이를 위해 국내에서 순수분리한 미소조류의 clonal culture를 관리하는 체계를 우선적으로 구축하여야 한다.

• 한국농업기계학회:학술대회논문집
• /
• 한국농업기계학회 1996년도 학술발표대회 논문집
• /
• pp.63-67
• /
• 1996

• Fisheries and Aquatic Sciences
• /
• 제14권4호
• /
• pp.323-332
• /
• 2011

To find seasonally optimal microalgae for mass culture of the rotifer Brachionus plicatilis, the growth rates of 12 microalgal species (two marine Chlorella spp., five marine Nannochloris spp., two marine Nannochloropsis spp., one estuarine Nannochloropsis sp., and two estuarine Chlorella spp.) were compared at $25^{\circ}C$ at 15 psu and 30 psu. Among these, six species showing high growth rates were chosen and examined again at high ($30^{\circ}C$ and $32^{\circ}C$) and low ($10^{\circ}C$) temperatures. Their amino and fatty acids and the dietary value of the rotifers that fed on each microalgal species were examined. Nannochloris sp. (KMMCC-119) and Chlorella vulgaris (KMMCC-120) showed the highest growth rates at temperatures over $30^{\circ}C$ and at $10^{\circ}C$, respectively. The growth rate of Nannochloris was higher than those of Chlorella and Nannochloropsis at high temperatures, but lower than those of the latter at low temperatures. The growth rate of rotifers fed on Nannochloropsis was highest and that of those fed on Chlorella was lowest. Levels of eicosapentaenoic acid and docosahexaenoic acid were highest in Nannochloropsis and lowest in Nannochloris. However, total amino acid content was highest in Nannochloris and lowest in Chlorella. In conclusion, Nannochloropsis sp. (KMMCC-33) was the best microalgal species for the mass culture of the rotifer. However, during high- or low-temperature seasons in which Nannochloropsis does not grow well, Nannochloris spp. (KMMCC-119, 395) and C. vulgaris (KMMCC-120) would adequately replace Nannochloropsis sp. (KMMCC-33).