• Bulletin of the Korean Chemical Society
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• 제34권3호
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• pp.735-742
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• 2013

Peptide nucleic acids (PNAs) that bind to complementary nucleic acid sequences with extraordinarily high affinity and sequence specificity can be used as antisense oligonucleotides against microRNAs, namely antagomir PNAs. However, methods for efficient cellular delivery must be developed for effective use of PNAs as therapeutic agents. Here, we demonstrate that antagomir PNAs can be delivered to hepatic cells by complementary DNA oligonucleotide and cationic liposomes containing galactosylated ceramide and a novel cationic lipid, DMKE (O,O'-dimyristyl-N-lysyl glutamate), through glycoprotein-mediated endocytosis. An antagomir PNA was designed to target miR-122, which is required for translation of the hepatitis C virus (HCV) genome in hepatocytes, and was hybridized to a DNA oligonucleotide for complexation with cationic liposome. The PNA-DNA hybrid molecules were efficiently internalized into hepatic cells by complexing with the galactosylated cationic liposome in vitro. Galactosylation of liposome significantly enhanced both lipoplex cell binding and PNA delivery to the hepatic cells. After 4-h incubation with galactosylated lipoplexes, PNAs were efficiently delivered into hepatic cells and HCV genome translation was suppressed more than 70% through sequestration of miR-122 in cytoplasm. PNAs were readily released from the PNA-DNA hybrid in the low pH environment of the endosome. The present study indicates that transfection of PNA-DNA hybrid molecules using galactosylated cationic liposomes can be used as an efficient non-viral carrier for antagomir PNAs targeted to hepatocytes.

• 한국융합학회논문지
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• 제11권8호
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• pp.237-245
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• 2020

본 연구의 목적은 자궁절제술이라는 외상사건을 경험한 대상자의 스트레스 대처, 배우자지지, 의도적 반추, 외상 후 성장 간의 관계를 파악하기 위해 시도되었다. 본 연구 대상자는 자궁절제술을 경험한 67명의 여성으로 수집된 자료는 t-검정, ANOVA 및 다중회귀분석을 사용하여 분석하였다. 본 연구결과 대상자의 외상 후 성장은 스트레스 대처 중 소극적 대처(r=0.27, p=.026), 적극적 대처(r=0.34, p=.004) 그리고 의도적 반추(r=0.46, p<.001)와 유의한 양의 상관관계가 있는 것으로 나타났으며, 소극적 대처는 적극적 대처(r=0.74, p<.001)와 유의한 양의 상관관계가 있는 것으로 나타났다. 자궁절제술을 경험한 여성의 외상 후 성장에 영향을 미치는 요인으로는 의도적 반추(β=0.42, p<.001)인 것으로 나타났고 이들의 총 설명력은 26%로 확인되었다(F=8.68, p<.001). 그러므로 본 연구의 결과를 토대로 자궁절제술을 가진 대상자의 외상 후 성장을 증진시키기 위해서는 의도적 반추를 증가하여 이들이 겪는 외상에 효과적으로 직면할 수 있도록 하는 중재 프로그램이 필요하다.

• 디지털융복합연구
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• 제19권11호
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• pp.545-553
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• 2021

본 연구는 사회복지전공 대학생의 죽음에 대한 태도의 영향을 미치는 요인을 파악하기 위한 서술적 조사 연구이다. 연구대상자는 2개 대학에 재학 중인 사회복지전공 대학생 179명을 대상으로 시행하였으며, 구조화된 설문지를 통해 자료 수집을 하였다. 수집한 자료는 기술통계와 t-test, ANOVA, Pearson correlations, 다중회귀분석으로 분석하였다. 연구결과 사회복지전공 대학생의 삶의 의미(r=.34, p=.001)와 삶의 만족도(r=.61, p<.001), 회복탄력성(r=.65, p<.001)과 죽음에 대한 태도는 정적 상관관계를 보였다. 위계적 회귀분석을 실시한 결과 학년(β=.21, p=.002)과 삶의 만족도(β=.22, p=.013)가 주요 영향요인이었으며, 이 모델은 20.2%의 설명력을 나타내었다. 연구결과를 통해 사회복지 전공 대학생의 죽음에 대한 태도를 긍정적으로 변화시킬 수 있는 상담 및 지지프로그램과 죽음 관련 교육 프로그램의 개발이 이루어져야 할 것이다.

• 생약학회지
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• 제44권1호
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• pp.47-52
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• 2013

We invesitagat the effects of antioxidant and suppression of MMP-1 in UV-irradiated human dermal fibroblasts (HDF) by schizandrin isolated from Schisandra chinensis. Ultrasonic technique was used to extract schizandrin from S. chinensis. The higher contents of schizandrin (6.34 mg/ml) in 70% EtOH extract obtained by ultrasonic treatment at 20 MHz for 2 h during 10 days. Schizandrin had antioxidant activities. Schizandrin (1-10 ${\mu}g/ml$) induced a significant dose-dependent decrease in the expression fo MMP-1 protein. These results suggest that schizandrin is a potential candidate for the prevention and treatment of skin aging.

• 성인간호학회지
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• 제21권5호
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• pp.529-537
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• 2009

Purpose: The purpose of this study was to investigate the correlation of sexual satisfaction and daily stress in breast cancer patients. Methods: Data was collected through self-administered questionnaires and analyzed by descriptive statistics, t-test, ANOVA and Pearson's correlation. Data survey was conducted with 500 conveniently selected breast cancer patients who visited the out patient department in 5 university hospitals in Seoul, Gyung-gi and Gang-won province. Results: The sexual satisfaction of breast cancer patients score was 31.34 and there were significant sexual satisfaction differences by age, education level, menopause and sexual activity frequency. Sexual satisfaction was negatively related with daily stress (r = -.177) especially personal stress (r = -.155), economic stress (r = -.138), stress of self (r = -.181), family stress (r = -.154) and stress about leisure (r = -.139). Conclusion: These findings are expected to make a contribution to creation of ideal sexual rehabilitation nursing interventions for breast cancer patients care nurse. Furthermore continuous and customized education and counseling programs can contribute to promote healthy sexual life for breast cancer patients.

• 환경생물
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• 제34권3호
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• pp.193-200
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• 2016

해산로티퍼 (Brachionus plicatilis)의 생존율 및 개체군 성장률을 사용하여 중금속 As, Cr 및 Pb에 대한 독성평가를 수행하였다. 중금속에 24시간 노출한 생존율은 As와 Cr의 30과 $150mg\;L^{-1}$ 농도에서 급격한 감소가 시작되어 농도의존적으로 감소하였으나, Pb에서는 영향이 나타나지 않았다. 중금 속에 72시간 노출한 개체군 성장률은 As, Cr 및 Pb의 5, 25 및 $50mg\;L^{-1}$ 농도에서 급격한 감소가 시작되어, 3종 중금속에서 모두 농도의존적으로 감소하는 경향을 보였다. As, Cr 및 Pb에 노출된 개체군 성장률의 $EC_{50}$ 값은 각각 12.98, 82.34 및 $110.14mg\;L^{-1}$이고 독성의 세기는 As>Cr>Pb로 생존율과 동일하였다. 또한 As, Cr 및 Pb에 대한 개체군 성장률의 NOEC는 각각 1, 12.5 및 $50mg\;L^{-1}$, LOEC는 각각 5, 25 및 $50mg\;L^{-1}$로 나타났다. 해양환경에서 각각의 LOEC 이상의 농도는 B. plicatilis에게 독성영향을 미칠 수 있는 농도로 판단되며, NOEC와 $EC_{50}$ 값은 WET test를 위한 혼합독성과 해양생태계 내 중금속독성에 대한 가이드라인으로 적절하게 활용될 수 있을 것이다.

• 대한간호학회지
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• 제34권7호
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• pp.1307-1314
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• 2004

Purpose: The purpose of this study was to identify the factors influencing Symptoms of Stress among hospital staff nurses. Method: Data was collected by questionnaires from 249 hospital staff nurses in three General Hospital. The data were analyzed using descriptive statistics, pearson correlation coefficients, and stepwise multiple regression. Results: The score of the symptoms of stress showed a significantly positive correlation with the score of work stress(r=.22, p=.00). The symptoms of stress showed a significantly negative correlation with the score of social support(r=-.28, p=.00), self efficacy(r=-.31, p=.00), and hardiness(r=-.24, p=.00). The most powerful predictor of symptoms of stress was social support and the variance explained was $16\%$. A combination of social support, ways of coping, and work stress account for $32\%$ of the variance in symptoms of stress among hospital staff nurses. Conclusion: This study suggests that social support, ways of coping, self efficacy, hardiness, and work stress are significantly influencing factors on symptoms of stress among hospital staff nurses.

• 한국염색가공학회지
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• 제34권2호
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• pp.102-108
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• 2022

The physicochemical properties of stainless steel fibers which were modified by oxygen plasma treatment were analyzed through microscopy and XPS analysis. The wettability of the surface of the stainless steel fiber was observed by measuring water contact angle to find out the effect of the plasma treatment time on the surface characteristics of the stainless steel fiber. In addition, in order to understand the effect of oxygen plasma treatment on the deterioration of the stainless steel fiber properties, the physical properties due to plasma treatment was investigated by measuring the weight reduction, tensile strength, elongation, tensile modulus of the stainless steel fibers according to the treatment time. As a result, the stainless steel fiber surface was etched by the oxygen plasma and the surface became more wettable by the introduction of hydrophilic functional groups. However the physical properties of the stainless steel fiber were not significantly deteriorated even if the surface of the stainless steel fiber made hydrophilic.

• 한국보건간호학회지
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• 제36권1호
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• pp.20-32
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• 2022

Purpose: This study aimed to investigate factors affecting handwashing practice among elementary school students by assessing the perception of coronavirus disease-19 (COVID-19) and characteristics related to knowledge, attitude, and practice of handwashing. Methods: The cross-sectional study design and an online self-reported survey were used in this study. The subjects were 211 elementary students at a school located in G province, Korea. Data were collected from January 4 to 8, 2021. The data were analyzed using SPSS version 25.0. Results: Mean scores for knowledge, attitude, and practice of handwashing were 17.09 (out of 20), 21.15 (out of 24), and 27.51 (out of 33), respectively. There were significant positive correlations between the perception of COVID-19 and knowledge of handwashing (r=.23, p=.001), between knowledge and attitude toward handwashing (r=.45, p<.001), between knowledge and practice of handwashing (r=.18, p=.010), and between attitude and practice of handwashing (r=.28, p<.001). The factors influencing handwashing practice were handwashing frequency at home (β=.39, p<.001), handwashing frequency at school (β=.18, p=.006), and attitude toward handwashing (β=.15, p=.026), which explained 34.2% of the variance. Conclusion: These results suggest a need to raise awareness of the importance of handwashing and develop an education program for infectious disease prevention.

• 동의생리병리학회지
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• 제34권6호
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• pp.348-354
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• 2020

Oryeong-san (ORS) is a traditional Korean herbal medicine widely used for renal associated diseases, composed of five medicine herbs; Atractylodes japonica Koidzumi, Cinnamomum cassia Presl, Polyporus umbellatus Fries, Poria cocos Wolf and Alisma orientale Juzepzuk. We studied to improve the convenience of intake and portability by developing modernized dosage forms, and examined the effect on anti-inflammation of ORS. In order to develop the tablet formulation of ORS (ORS-F), the tablets were evaluated on the basis of physical characteristics include diameter, thickness, weight variation, hardness, friability and disintegration. To analyze the marker components of ORS-F, eight index markers from five herbal medicines were chosen. And the method using high performance liquid chromatography (HPLC) with diode-array detector method was established for the simultaneous analysis. The biological activities were examined the effect of ORS-F on pro-inflammation mediated by LPS-stimulation. The production of nitric oxide (NO) and cytokines were determined by reacting cultured medium with griess reagent and enzyme-linked immunosorbent assay (ELISA). The expression of cyclooxygenase-2 (COX-2) and inducible NO synthase (iNOS) were investigated by Western blot and RT-PCR. The anti-oxidant activities of OJS-F increased markedly, in a dose-dependent manner. and, The total phenolic compound and flavonoids contents of OJS-F were 10.20±0.09 ㎍/㎎ and 12.86±0.86 ㎍/㎎. OJS-F which is LPS has diminished in the LPS-induced release of inflammatory mediators (NO, iNOS, COX2 and PGE2) and pro-inflammatory cytokines (TNF-α, IL-6 and IL-1β) from the RAW264.7 macrophages. Therefore, the developed formulation for tablet of ORS-F provide efficiency and usability, and indicated effect of anti-inflammation.