Browse > Article
http://dx.doi.org/10.15188/kjopp.2020.12.34.6.348

Effect of Anti-inflammation on Oryeong-san Formulation for Mix Extract Tablet  

Kim, Se Jin (Korean Medicine R&D Team 2, National Institute for Korean Medicine Development)
Leem, Hyun Hee (Korean Medicine R&D Team 2, National Institute for Korean Medicine Development)
Nam, Won Hee (Korean Medicine R&D Team 2, National Institute for Korean Medicine Development)
Son, Su Mi (Korean Medicine R&D Team 2, National Institute for Korean Medicine Development)
Choi, Hye Min (Korean Medicine R&D Team 2, National Institute for Korean Medicine Development)
Kim, Myung Jin (Korean Medicine R&D Team 2, National Institute for Korean Medicine Development)
Kim, Jung Ok (Korean Medicine R&D Team 2, National Institute for Korean Medicine Development)
Lee, Hwa Dong (Korean Medicine R&D Team 2, National Institute for Korean Medicine Development)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.34, no.6, 2020 , pp. 348-354 More about this Journal
Abstract
Oryeong-san (ORS) is a traditional Korean herbal medicine widely used for renal associated diseases, composed of five medicine herbs; Atractylodes japonica Koidzumi, Cinnamomum cassia Presl, Polyporus umbellatus Fries, Poria cocos Wolf and Alisma orientale Juzepzuk. We studied to improve the convenience of intake and portability by developing modernized dosage forms, and examined the effect on anti-inflammation of ORS. In order to develop the tablet formulation of ORS (ORS-F), the tablets were evaluated on the basis of physical characteristics include diameter, thickness, weight variation, hardness, friability and disintegration. To analyze the marker components of ORS-F, eight index markers from five herbal medicines were chosen. And the method using high performance liquid chromatography (HPLC) with diode-array detector method was established for the simultaneous analysis. The biological activities were examined the effect of ORS-F on pro-inflammation mediated by LPS-stimulation. The production of nitric oxide (NO) and cytokines were determined by reacting cultured medium with griess reagent and enzyme-linked immunosorbent assay (ELISA). The expression of cyclooxygenase-2 (COX-2) and inducible NO synthase (iNOS) were investigated by Western blot and RT-PCR. The anti-oxidant activities of OJS-F increased markedly, in a dose-dependent manner. and, The total phenolic compound and flavonoids contents of OJS-F were 10.20±0.09 ㎍/㎎ and 12.86±0.86 ㎍/㎎. OJS-F which is LPS has diminished in the LPS-induced release of inflammatory mediators (NO, iNOS, COX2 and PGE2) and pro-inflammatory cytokines (TNF-α, IL-6 and IL-1β) from the RAW264.7 macrophages. Therefore, the developed formulation for tablet of ORS-F provide efficiency and usability, and indicated effect of anti-inflammation.
Keywords
Oryeong-san (ORS)(五 ?散 ); Tablet; Simultaneous analysis; Anti-inflammation; Cytokines;
Citations & Related Records
Times Cited By KSCI : 6  (Citation Analysis)
연도 인용수 순위
1 Kim Y, Kim OS, Seo CS, Shin HK. Comparison of the ingredient quantities and biological activities of Hwangryunhaedok-tang (Hwanglianjiedu-tang) decoction and commercial extractive granules. Kor. J. Pharmacogn. 2013;44:22-9.
2 Kim OS, Seo CS, Kim Y, Shin HK. Comparative study on chemical composition and biological activities of Samchulkunbi-tang decoction and commercial herbal medicine. Formula Science. 2012;20:1-11.
3 Biosis MS. Antioxidant determinations by the use a stable free radical. Nature. 1958;181:1199-200.   DOI
4 Singleton VL, Rossi JA. Colorimetry of Total Phenolics with Phosphomolybdic- Phosphotungstic Acid Reagents. Am. J. Enol. Vitic. 1965;16:144-58.
5 Kobayachi H, Takahachi K, Mizuno N, Kutsuna H, Takahachi M. An alternative approach to atopic dermatitis: part I-case-series presentation. eCAM. 2004;1:49-62.
6 Choi SB, Bae GS. Jo IJ, Park KC, Seo SH, Kim DG, Shin JY, Gwak TS, Lee JH, Lee GS, Park SJ, Song HS. The anti-inflammatory effect of Lithospermum Erythrorhizon on lipopolysaccharide-induced inflammatory response in RAW 264.7 cells. Kor. J. Herbology. 2013;28:67-73.   DOI
7 Kwqamata H, Ochiai H, Mantani N, terasawa K. Enhanced expression of inducible nitric oxied RAW264.7 cells, a murine macrophage cell line. Am J Chin Med. 2000;28:217-26.   DOI
8 Chiou WF, Chou CJ, Chen CF.) Camptothecin suppresses nitric oxied biosythesis in RAW264.7 macrophages. Life Sci. 2001;69:625-35.   DOI
9 Oh YC, Jeong YH, Ha JH, Cho WK, Ma JY. Oryeongsan inibits LPS-induced production of inflammatory mediators via blockade of the NF-kappaB, MAPK pathways and eads to HO-1 induction in macrophage cells. BMC complement Altern Med. 2014;14:242-53.   DOI
10 Park J, Lim EK. Research about application of Shanghanlun disease pattern identification diagnostic system to 2 cases treated by Oreong-san. J of KMediACS. 2018;10:81-94.
11 Jeon WY, Lee MY, Shin IS, Lim HS, Shin HK. Protective effects of the traditional herbal formula Oryeongsan water extract on ethanol-induced acute gastric mucosal injury in rats. Evid Based Complement Alternat Med. 2012:2012:438191.
12 Park JK. A case report of primary insomnia treated by Oryeong-san decoction. J of KMediACS. 2015;7:45-52.
13 Heo J. A case report of dysmenorrhea treated by Oryeong-san based on Shanghanlun provisions. J of KMediACS. 2014;6:63-70.
14 Kim JH, Shin HK. Analysis of biological experiment on Oryeong-san (Wuling-san). Korean J. Orient. Int. Med. 2012;33:69-82.
15 Park SJ, Kim SH, Kim KS, Kim HS, Lee AY, Kim HK, Kim YK. A quantitative analysis of marker compounds in single herb extracts by the standard of KHP. Kor. J. Herbology. 2014;29:35-42.
16 Baek KY, Kim SJ, Kim JB, Lee JH, Moon SO, Lee HD. Comparison of marker componenets and bioloical acivities of socheongryong-tang by different exract methods. Kor. J. Pharmacogn. 2018;49:349-61.
17 Choi SM, Kim BW. Theoretical chemical reaction for herb medicine. J. Kor. Pharmacopuncture instit. 2002;5:116-19.   DOI
18 Son S. M. (2007) Demand and preference for the modification of dosage forms of oriental herbal medicines by 20s-30s younger consumers. M.S Thesis. Chung-Ang Univ; 2007.
19 Choi SI, Ahn EJ, Lee MY. Research trend for the formulation of traditional chinese medicine. Kor. Herb. Med. Inf. 2016;4:11-9.
20 Kim SJ, Lee JB, Choi HM, Lee HY, Kim MJ, Lee MH, Kim JO, Lee HD. Evaluation on Pharmacological effects and component analysis of hwanggeumjakyak-tang formulation for tablet. Kor. J. Pharmacogn. 2018;49:362-71.
21 Jee EH, Kim HJ, Jeong SH, Moon JH, Jang YP. Assessment of quality variance among commercial Hwang-Ryun-Hae-Dok-Tang by simultaneous analysis of characterizing copounds. Kor. J. Pharmacogn. 2010;41:161-5.
22 Bishop-Bailey D, Calatayud S, Warner TD, Hla T, Michell A. Prostaglandins and the regulation of tumor growth. J Environ Pathol Tox Oncol. 2002;21:93-101.
23 Kim MS, Jeong JS, Lee HY, Ju YS, Bae GS, Seo SW, Cho IJ, Park SJ, Song HJ. The anti-inflammatory effect of Achyranthes japonica on lipopolysaccharide-induced inflammatory activity in murine macrophages. Kor. J. Herbology. 2011;26:51-7.
24 Jung YS, Eun CS, Jung YT, Kim HJ, YU MH. Anti-Inflammatory Efects of Picrasma Quasioides (D.DON) BENN Leaves Extrac. Journal of Life Science. 2013;23:629-36.   DOI
25 Rocca B, FitzGerald GA. Cyclooxygenases and rostaglandins: shaping up the immune response. International mmunopharmacology. 2002;2:603-30.   DOI