• Title/Summary/Keyword: miR-29a-3p

Search Result 100, Processing Time 0.029 seconds

Isolation and Characterization of a Protease-Producing Bacterium, Bacillus amyloliquefaciens P27 from Meju as a Probiotic Starter for Fermented Meat Products

  • Lee, Mi-Sun;Lee, Na-Kyoung;Chang, Kyung-Hoon;Choi, Shin-Yang;Song, Chi-Kwang;Paik, Hyun-Dong
    • Food Science of Animal Resources
    • /
    • v.30 no.5
    • /
    • pp.804-810
    • /
    • 2010
  • This study was performed to select protease-producing Bacillus sp. as a potential probiotic starter for fermented meat products. In order to isolate protease-producing bacterium from meju, measured the diameter of the clear zone on agar plate (TSA, 1% (w/v) skim milk) and analyzed for intracellular protease activity, then 10 Bacillus-like strains were isolated. Three Bacillus-like strains (P19, P27, and P33) among 10 strains were able to tolerate in acidic condition (TSB, pH 2.5, 2 h incubation). These 3 strains were showed antimicrobial activity against food-borne pathogenic bacteria. These vegetative cells of 3 strains were showed a survival rate of 0.04% to 0.08% under the artificial gastric acidic condition (TSB, pH 2.5 with 1% (w/v) pepsin), but spore-forming cells were 56.29% to 84.77%. Vegetative cells of 3 strains were the least bile-resistant, while spore-forming cells of 3 strains showed higher survival rate more than 76% under artificial bile condition (TSB, 0.1% (w/v) oxgall bile). In these strains, P27 strain was finally selected as a good probiotic strain. P27 strain was tentatively identified as Bacillus amyloliquefaciens by API CHB kit and 16S rDNA sequence analysis. The results of this study suggest that B. amyloliquefaciens P27 can be used as a potential probiotic starter for fermented meat product.

Factors Influencing Loneliness in Elderly Living in Nursing Homes (요양시설 노인의 외로움에 영향을 미치는 요인)

  • Kim, In-Sook;Kim, Mi-Sook;Kang, Hee-Young
    • Journal of Korean Academy of Fundamentals of Nursing
    • /
    • v.17 no.3
    • /
    • pp.391-399
    • /
    • 2010
  • Purpose: This study was done to examine factors influencing loneliness in the elderly living in nursing homes. Method: The participants for this study were 150 adults, age 60 or over who had lived for one year or longer in two nursing homes in Gwangju. The questionnaires consisted of measurements of activities of daily living (ADL), social support and loneliness. Frequencies, percentages and means were used with Pearson correlation coefficients, t-test, ANOVA, and Scheffe test to analyze the data. The analysis was done with SPSS 12.0 Stepwise multiple regression was used to identify significant factors influencing loneliness in the elderly living in nursing homes. Results: Significant correlations were found between level of ADL and loneliness (r=-0.379, p<.001), support from friends and loneliness (r=-0.472, p<.001), and support from significant others and loneliness (r=-0.449, p<.001). Loneliness was significantly influenced by support from friends, ADL, and support from significant others. This regression model explained 29% of the variances in loneliness. Conclusion: The findings in this study suggest that improvement in support from friends and ADL can lead to a reduction in loneliness in the elderly living in nursing homes.

Influencing Factors of Nursing Performance for Life Care of Delirium Patients among Nursing Students (섬망환자의 라이프케어를 위한 간호학생의 섬망간호 수행 영향요인)

  • Oh, Hyo-Sook;Chang, Mi-Young
    • Journal of Korea Entertainment Industry Association
    • /
    • v.13 no.4
    • /
    • pp.401-410
    • /
    • 2019
  • This study was conducted to identify factors affecting nursing performance of delirium among nursing students. A total of 252 fourth year students were recruited from nursing department in Gwangju. Structured questionnaire was self-administrated from April to September, 2017. The used statistical analysis were t-test, ANOVA, Pearson's coefficient and multiple regression analysis. Knowledge of delirium 29.0±7.24, self-confidence in the care for delirium 71.65±28.55 and nursing performance level for patients with delirium was 41.16±8.97. Nursing performance of delirium had significant positive correlations with delirium knowledge, self-confidence of delirium care. In multiple regression analysis, nursing experience for delirium patients, self-confidence of delirium care, practice experience in intensive care unit, use of nursing diagnosis related to delirium, and satisfaction of clinical practice were significant factors of nursing performance of delirium explaining 29.8% of the variables. In conclusion, to enhance nursing performance of delirium, it is necessary to develop educational program for increasing nursing experience for delirium patients during clinical practice and self-confidence of delirium care.

The Impact on Supportive Education on the Effectiveness of Breast Self-examination among College Women and Correlate to Self-Efficacy (여대생에게 실시한 지지교육이 유방자가검진 교육에 미치는 영향과 자기효능과의 관계연구)

  • Lee, Mi-Wha;Choi, In-Hee
    • Korean Journal of Adult Nursing
    • /
    • v.13 no.3
    • /
    • pp.397-408
    • /
    • 2001
  • Currently, breast cancer ranks third among women' s cancers, and as its incidence is increasing, the incidence age is also becoming lower. Therefore it is necessary to address breast cancer for women in their twenties. As there is no way presently to prevent breast cancer, it is imperative that women take available interventions against predisposing factors. It is thus advisable that women acquire the necessary skills to recognize their own health status. The purposes of this study were to identify the effects of education on breast self-examination (BSE) through supportive education among college women during the period from August 2000 to February 2001, and to attempt to design an effective BSE educational program. The first class was implemented through lectures, pamphlets, videotapes, breast palpation on cloths, demonstration and practice for identification of breast masses through palpation using breast model. Supportive education was implemented bimonthly to the experimental group, and effects of the education between experimental and control groups were compared 6 months later. The results of this study are summarized as follows: 1. The effects on supportive education of college women in BSE 1) The mean score of retained knowledge about breast cancer and BSE was 30.88 in the experimental group, and 29.66 in the control group and significantly greater in the experimental group than in the control group (t= -2.062, p= 0.041). 2) Frequency of BSE practice was significantly greater in the experimental group than in the control group($\chi^2=0.045$, p=0,012). 3) The mean accuracy score in BSE practice was 19.10 in the experimental group, and 18.29 in the control group; accuracy was higher in the experimental group than in the control(t= -2.035, p= 0.444). 4) The mean score of self-efficacy was 35.05 in the experimental group, and 31.22 in the control group. The experimental group mean score was higher(t=-3.016, p=0.003). 2. There was a statistically significant correlation between self-efficacy and accuracy of BSE(r=0.447, p=0.000), knowledge of breast cancer and BSE(r= 0.306, p=0.000) and frequency of BSE(r=0,259, p=0,002) but no significant correlation between knowledge of breast cancer and BSE and frequency of BSE (r=0.071, p=0.403). On the basis of this study, periodic supportive education can increase knowledge of breast cancer and BSE, frequency of BSE, accuracy of BSE and self-efficacy. Suggestions: 1. There is a need to compare the effects of individual programs to acquire BSE behaviors in the young. 2. Further research is needed to test the continuity of the effects of BSE education.

  • PDF

A study on the Influence of Empathy Ability and Sense of Humor on the Communication Ability of Psychiatric Nurses (정신간호사의 공감능력과 유머감각이 의사소통능력에 미치는 영향)

  • Park, Seo Hyun;Kim, Mi Young
    • The Journal of the Convergence on Culture Technology
    • /
    • v.7 no.2
    • /
    • pp.1-7
    • /
    • 2021
  • The purpose of this descriptive study was to examine the degree and the relationship among empathy ability, sense of humor and communication ability of psychiatric nurses and to understand factors which influence on communication ability. Data were collected from 219 psychiatric nurses in B and G city and analyzed by t-test, ANOVA, Pearson correlation coefficient, and multiple regression using SPSS/WIN 22.0. The degree of communication ability in psychiatric nurses was 3.59±.43. There were significant differences in communication ability with respect to sense of humor(r=.53, p<.001), and also between communication ability and empathy ability(r=.39, p<.001), with an explanatory power of 34.4%(F=29.56, p<.001). In conclusion, these findings indicate that the higher sense of humor and empathy ability of psychiatric nurses, the higher communication ability. Accordingly, to improve the communication ability of psychiatric nurses, educational programs to improve sense of humor and empathy ability of psychiatric nurses should be developed and utilized

Treatment Kinetics of Wastewater and Morphological Characteristics of Biofilm in Upflow Biobead® Process (상향류식 바이오비드 공법을 이용한 오·폐수 처리특성 및 부착 생물막의 형태적 특징)

  • Yum, Kyu-Jin;Lee, Jeong-Hun;Kim, Sun-Mi;Choi, Weon-Seok
    • Journal of Korean Society on Water Environment
    • /
    • v.18 no.2
    • /
    • pp.201-212
    • /
    • 2002
  • The objective of this study was to investigate the treatment efficiency, kinetics, and morphological characteristics of biofilm in upflow $Biobead^{(R)}$ process, a kind of biological aerated filter(BAF). The $Biobead^{(R)}$ system showed high removal rates of $COD_{Mn}$(76~83%), $BOD_5$(67~88%) and SS(71~91%) for food wastewater with high salt concentration ($>4,000mg/{\ell}$) under short reaction times(2~3hrs). Even at aerobic condition, the system had high treatment efficiency for both T-N (51~63%) and T-P(62~81%). The removal kinetics of $COD_{Mn}$, $BOD_5$, T-N, T-P, and $Cl^-$ in the $Biobead^{(R)}$ system showed a plug-flow pattern with reaction rate constants($hr^{-1}$) of 0.58, 0.63, 0,30, 0.48, and 0.38 respectively. A backwashing process to remove excess biomass and filtered solids was needed at least once during 22-hour operation at $0.5kg\;BOD\;m^{-3}{\cdot}d^{-1}$ loading. At the higher loading($1.0kg\;BOD\;m^{-3}{\cdot}d^{-1}$) the backwashing interval was shorten by 8 hours. The COD, BOD, T-N, and T-P were removed from 43 to 66% only by aerobic biodegradation. The SS was removed over 70% by the filtering of $Biobead^{(R)}$ media in the treatment system. The first one of three serial Biobead reactors showed the highest removal values for $COD_{\alpha}$(52.3%), $COD_{Mn}$(38.8%), BOD(62.5%), and T-N(40.0%). The SS and T-P had the highest removal values(47.5% and 29.2%) at the second one of the serial reactors. The biofilm had non-homogeneous spatial distribution and the colonies were embedded in the sunk area of the Biobead. The thickness of the biofilm was very thin ($5.0{\sim}29.4{\mu}m$) compared to the biofilm thickness($200{\sim}300{\mu}m$) used in other BAF systems.

Knowledge, attitude, and preventive action on COVID-19 infectious disease of cosmetology major and arts and physical education college students (뷰티전공과 예체능전공 대학생의 COVID-19에 대한 지식, 태도, 예방실천행위)

  • Ahn, Mi Jeung;Park, Sun Ju
    • Journal of the Korea Fashion and Costume Design Association
    • /
    • v.23 no.4
    • /
    • pp.73-80
    • /
    • 2021
  • This study is a descriptive cross-sectional study to confirm the knowledge, attitudes, and preventive practice behaviors of university students concerning COVID-19 and to identify the factors that influence preventive practice behaviors. The number of study subjects was calculated using G*power program 3.19. The data collected from the study were analyzed using SPSS/WIN version 25. The subject's characteristics, knowledge, attitude, and preventive practices against COVID-19 were analyzed with descriptive statistics, and the difference in knowledge, attitude, and prevention practices against COVID-19 according to the subject's characteristics was evaluated using an independent t-test, ANOVA, and post-hoc test was conducted using a Scheffé test. Correlations of knowledge, attitude, and prevention practices against COVID-19 were analyzed using Pearson Correlation coefficients, and factors affecting COVID-19 prevention practices were analyzed using stepwise multiple regression. The number of subjects analyzed in the study was 232. The general characteristics of the study subjects were 78 males (33.7 %) and 154 females (66.3 %). The subject's knowledge score about COVID-19 was 85.13±6.22 out of 100 points, and the attitude score toward COVID-19 was 12.47±1.31 out of 14 points. The prevention practice score for COVID-19 was 29.36±3.42 out of 32 points. As a result of the study, knowledge about COVID-19 was found to have a significant positive correlation with attitude (r=.34, p<.001); COVID-19 prevention practice behavior was found to have a significant positive correlation with knowledge (r=.54, p<.001), and attitude (r=.62, p<.001). The main factors influencing the COVID-19 prevention practice behavior were knowledge, attitude, and major (Cosmetology major).

A Study on Characteristics and Predictions of Seasonal Chlorophyll-a using Bayseian Regression in Paldang Watershed (베이지안 추정을 이용한 팔당호 유역의 계절별 클로로필a 예측 및 오염특성 연구)

  • Kim, Mi-Ah;Shin, Yuna;Kim, Kyunghyun;Heo, Tae-Young;Yoo, Moonkyu;Lee, Su-Woong
    • Journal of Korean Society on Water Environment
    • /
    • v.29 no.6
    • /
    • pp.832-841
    • /
    • 2013
  • In recent years, eutrophication in the Paldang Lake has become one of the major environmental problems in Korea as it may threaten drinking water safety and human health. Thus it is important to understand the phenomena and predict the time and magnitude of algal blooms for applying adequate algal reduction measures. This study performed seasonal water quality assessment and chlorophyll-a prediction using Bayseian simple/multiple linear regression analysis. Bayseian regression analysis could be a useful tool to overcome limitations of conventional regression analysis. Also it can consider uncertainty in prediction by using posterior distribution. Generally, chlorophyll-a of a P2(Paldang Dam 2) site showed high concentration in spring and it was similar to that of P4(Paldang Dam 4) site. For the development of Bayseian model, we performed seasonal correlation. As a result, chlorophyll-a of a P2 site had a high correlation with P5(Paldang Dam 5) site in spring (r = 0.786, p<0.05) and with P4 in winter (r = 0.843, p<0.05). Based on the DIC (Deviance Information Criterion) value, critical explanatory variables of the best fitting Bayesian linear regression model were selected as a $PO_4-P$ (P2), Chlorophyll-a (P5) in spring, $NH_3-N$ (P2), Chlorophyll-a (P4), $NH_3-N$ (P4) in summer, DTP (P2), outflow (P2), TP (P3), TP (P4) fall, COD (P2), Chl-a (P4) and COD (P4) in winter. The results of chlorophyll-a prediction showed relatively high $R^2$ and low RMSE values in summer and winter.

Self Care Behavior of Hemodialysis Patients (혈액투석환자의 자가간호행위)

  • Choi, Mi-Kyoung;Choe, Myoung-Ae
    • Journal of Korean Biological Nursing Science
    • /
    • v.9 no.2
    • /
    • pp.105-117
    • /
    • 2007
  • Purpose: The purpose of this study was two folds: first, to identify the level of self care behavior of the hemodialysis patients and second, to find the correlation between the self care behavior and the physiologic indices. Method: The subjects were 52 hemodialysis patients, male and female, who have regularly received hemodialysis dialysis at the Dialysis Room in a leading teaching hospital, Seoul. The patients responded to the self care behavior questionnaires including their socio-demographic characteristics. The respondents have regularly recorded the self care log book. The physiologic indices, clinical characteristics related to the disease and hemodialysis were collected by the chart review. Result: The mean score of the self care behavior was 3.46. The mean score of the self care behavior on categories demonstrated as follows: medication 4.29, fistula management 4.13, management of physical problem 3.71, diet 3.28, exercise and rest 3.22, blood pressure and body weight management 2.97 and social adjustment 2.05 in order. Thirty patients managed discomfort of their fistula. Eleven patients took exercise for 0.5-1 hr/week. Thirty patients measured their body weight daily and thirty two measured their blood pressure daily. The score of self care behavior was significantly correlated with the mean weight gains between the dialysis sessions(r=-.312, p=.05). The mean weight gains between dialysis sessions was found to be high as the level of serum phosphorus and potassium increased(r=-.316, p=.05, r=-.465, p=.01). Conclusion: The result suggests that nursing intervention to the hemodialysis patients to improve self care behavior should be encouraged and further developed.

  • PDF

Influence of Infection Control Fatigue and Social Support on Nurses' Burnout During the Coronavirus Disease 2019 Pandemic (코로나19 대유행에 따른 간호사의 감염관리 피로도, 사회적 지지가 소진에 미치는 영향)

  • Kang, Hee Jeong;Lee, Mi Hyang;Lim, Hyo Nam;Lee, Kyung Hwa
    • Journal of Korean Clinical Nursing Research
    • /
    • v.28 no.3
    • /
    • pp.299-307
    • /
    • 2022
  • Purpose: The purpose of this study was to understand the effects of infection control fatigue and social support on burnout among nurses during the Coronavirus Disease 2019 (COVID-19) pandemic. Methods: This study is a descriptive survey study designed to confirm the effects of nurses' infection control fatigue and social support on burnout among nurses during the COVID-19 pandemic. The collected data were analyzed using frequency, percentage, mean, standard devia analyzed using frequency, percentage, mean, standard deviation, independent t-test, One-way ANOVA, Scheffe test, Pearson's correlation coefficient, and stepwise multiple regression analysis using SPSS Statistics 26.0. Results: An analysis of the correlations between fatigue from infection control, social support, and burnout showed a positive correlation between burnout and fatigue from infection control (r=.39, p<.001), and a negative correlation between burnout and social support (r=-.29, p<.001). Conflict and lack of support due to uncertain circumstances (β=.51, p<.001), support from supervisor's (β=-.22, p<.001), and experience of infection management education during the previous 1year (β=-.15, p=.007) were identified as the factors that influenced burnout among nurses, and explained 39.0% of the variance in burnout. Conclusion: The results of this study demonstrate that fatigue from infection control and social support influence burnout levels among nurses, which suggests the need to establish a new kind of work culture. Additionally, the findings call for the development and implementation of interventional programs that can reduce fatigue from infection control and increase social support for nurses.