• The Journal of Korean Society for School & Community Health Education
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• v.22 no.4
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• pp.11-24
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• 2021

Purpose: This study aims to explore the mediating effect of the health self-efficacy and activity confidence in the relationship between body image and health promoting behavior in middle school girls. Methods: A descriptive correlational design was used. Participants were 142 in middle school girls. The questionnaire consisted of measuring tools for body image, health promoting behavior, health self-efficacy and activity confidence. The collected data were analyzed using descriptive statistics, independent t-test, one-way ANOVA, Pearson's correlation coefficient and the PROCESS Macro for SPSS 23.0 to verify the multiple mediated effect with a parallel structure. Results: Body image showed a significant positive correlation with health self-efficacy (r=.28, p=.001), activity confidence (r=.45, p<.001) and health promoting behavior (r=.37, p<.001). Health self-efficacy was also significantly correlated with activity confidence (r=.51, p<.001) and health promoting behavior (r=.63, p<.001). Activity confidence was significantly health promoting behavior (r=.67, p<.001). Furthermore, health self-efficacy (B=.35, p<.001) and activity confidence (B=.38, p<.001) showed a mediation effect on the relationship between body image and health promoting behavior. Conclusion: The impact of body image on health promoting behavior in middle school girls was mediated by health self-efficacy and activity confidence. Result suggest that strategies for improving health self-efficacy and activity confidence in middle school girls should be considered when developing an educational program for enhancing their health promoting behavior.

• Journal of Ginseng Research
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• v.41 no.4
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• pp.477-486
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• 2017

Background: Our previous studies have demonstrated that ginsenoside-Rg1 can promote angiogenesis in vitro and in vivo through activation of the glucocorticoid receptor (GR). Furthermore, microRNA (miRNA) expression profiling has shown that Rg1 can modulate the expression of a subset of miRNAs to induce angiogenesis. Moreover, Rb1 was shown to be antiangiogenic through activation of a different pathway. These studies highlight the important functions of miRNAs on ginseng-regulated physiological processes. The aim of this study was to determine the angiogenic properties of Korean Red Ginseng extract (KGE). Methods and Results: Combining in vitro and in vivo data, KGE at $500{\mu}g/mL$ was found to induce angiogenesis. According to the miRNA sequencing, 484 differentially expressed miRNAs were found to be affected by KGE. Among them, angiogenic-related miRNAs; miR-15b, -23a, -214, and -377 were suppressed by KGE. Meanwhile, their corresponding angiogenic proteins were stimulated, including vascular endothelial growth factor, vascular endothelial growth factor receptor-2, endothelial nitric oxide synthase, and MET transmembrane tyrosine kinase. The miRNAs-regulated signaling pathways of KGE were then found by Cignal 45-Pathway Reporter Array, proving that KGE could activate GR. Conclusion: KGE was found capable of inducing angiogenesis both in vivo and in vitro models through activating GR. This study provides a valuable insight into the angiogenic mechanisms depicted by KGE in relation to specific miRNAs.

• Journal of Korean Medicine for Obesity Research
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• v.5 no.1
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• pp.57-66
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• 2005

Objectives : The purpose of this study is to examine accuracy of predicted resting energy expenditure (REE), relationship fat free mass (FFM) and REE. Methods : 60 normal, obese women $(body\;mass\;index\;{\geq}25kg/m^2)$ were recruited for this study, they had low calorie diet for 8 weeks. At week 0, 4, and 8, REE was measured by MedGem (indirect calorimeter), Bioelectrical impedance analysis (BIA) using Cunningham equation, and Harris-Benedict (H-B) equation, FFM was also measured by BIA. Results : The REE predicted by BIA was lower than the REE measured by MedGem (MG) in every measurement. The REE predicted by H-B equation predicted REE was lower than that of MG in the second measurement (p<0.01). The REE measured by MedGem was declined after 8 weeks, BIA and H-B equation predicted REE were declined after 4 weeks (p<0.01). H-B equation predicted REE had more significant correlation with the REE measured by MedGem than that of BIA. There was significant correlation between measured REE and FFM, but measured REE declined after 8 weeks, FFM declined after 4 weeks (p<0.01). We derived a prediction equation as follows : REE = 108.36+31.42 (FFM), $R^2=0.23$.

• Journal of Korean Critical Care Nursing
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• v.10 no.1
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• pp.63-74
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• 2017

Purpose: The purpose of this study was to examine the effect of professional autonomy, organizational commitment, and perceived patient safety culture on patient safety management activities of nurses in medium and small-sized hospitals. Methods: A cross-sectional design was employed. Self-reported questionnaires were completed by 121 nurses with at least 3 months of working experience in medium and small-sized hospitals located in B city. Data were analyzed using descriptive statistics, a t-test, a one-way ANOVA, Pearson correlation coefficients, and a multiple regression analysis. Results: Professional autonomy (r=.22, p=.016), organizational commitment (r=.34, p<.001), and perceived patient safety culture (r=.55, p<.001) had a statistically significant positive correlation with patient safety management activities. The factors that might affect patient safety management activities were professional autonomy (${\beta}=.23$, p=.003) and perceived patient safety culture (${\beta}=.55$, p<.001). The explanatory power of these factors for patient safety management activities was 33.5% (F=21.19, p<.001). Conclusions: The development of repetitive and continuous education programs is needed to improve a nurse's professional autonomy and perceived patient safety culture.

• Journal of East-West Nursing Research
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• v.28 no.2
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• pp.104-111
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• 2022

Purpose: This study aimed to investigate factors affecting the problem solving ability of nursing students. Methods: The data in this study was collected through structured questionnaires from September 1st to October 30th, 2021. Study participants were 172 fourth grade nursing students at four departments of nursing located in B and K city. IBM SPSS WIN v 21.0. program with t-test, ANOVA, Scheffe' test, Pearson's correlation coefficients, and hierarchical regression was used for data analysis. Results: The mean scores of academic self-efficacy was 3.88±051, empathy was 3.76±0.33, communication skills were 3.94±0.48, and problem solving ability was 3.70±0.40. There were significantly positive correlations between problem solving ability and academic self-efficacy (r=.45, p=<.001), empathy (r=.51, p=<.001), communication skills (r=.52, p=<.001), Factors affecting participants' problem solving ability were empathy (β=.31, p=<.001), and communication skills (β=.23, p=.006), which explained about 38.4% of the problem solving ability. Conclusion: In this study, it is necessary to develop curricular and non curricular nursing program that can strengthen empathy and good communication skills together when developing programs that increase problem solving ability.

• The Plant Pathology Journal
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• v.32 no.6
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• pp.575-579
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• 2016

Burkholderia glumae (bacterial grain rot), Xanthomonas oryzae pv. oryzae (bacterial leaf blight), and Acidovorax avenae subsp. avenae (bacterial brown stripe) are major seedborne pathogens of rice. Based on the 16S and 23S rDNA sequences for A. avenae subsp. avenae and B. glumae, and transposase A gene sequence for X. oryzae pv. oryzae, three sets of primers had been designed to produce 402 bp for B. glumae, 490 bp for X. oryzae, and 290 bp for A. avenae subsp. avenae with the $63^{\circ}C$ as an optimum annealing temperature. Samples collected from naturally infected fields were detected with two bacteria, B. glumae and A. avenae subsp. avenae but X. oryzae pv. oryzae was not detected. This assay can be used to identify pathogens directly from infected seeds, and will be an effective tool for the identification of the three pathogens in rice plants.

• Animal cells and systems
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• v.4 no.1
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• pp.23-30
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• 2000

Horizontal starch gel electrophoresis for 29 populations (n=543) of the wrinkled frog, Rana rugosa, from Korea and Japan was peformed to assess the degree of genic variation and genetic diversity, and to understand the biogeographic pattern of distribution and speciation. A sum of 22 presumptive loci was screened from 17 enzymes and general proteins. Four loci, Aco, Est-3, Me-2, and Pgm, demonstrated high levels of polymorphism. The degree of average genetic variation of R. rugosa was P=22.7% (9.1-40.9%), Ho=0.086 (0.048-0.165) and He=0.090 (0.042-0.168). In the south-eastern region of the Korean peninsula (Chongsong, Yongchon, Ulsan, Kyongju, Pohang, yongdok and Ulchin), a few unique alleles in the Mpi locus were detected and their biogeographic implications were considered. The degree of genetic differentiation among the Korean populations was moderate (S=0.900), whereas the degree of genetic diversity between Korean and Japanese populations was notably high (S=0.687, D=0.293). This result corresponds with the data obtained by the mitochondrial cytochrome b gene sequence (Lee et al., 1999) suggesting that the Korean and Japanese R. rugosa might have evolved a specific level of genetic differentiation since their geographic isolation.

• The Journal of Korean Society for School & Community Health Education
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• v.23 no.2
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• pp.39-50
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• 2022

Objectives: This study aimed to confirm the association between self-determination and self-directed learning among Korean nursing students, as well as the mediating effect of academic self-efficacy. Methods: Data from 139 nurse students were surveyed in this descriptive cross-sectional study. They were collected from Oct 1 to 30, 2020, using self-report questionnaires. The collected data were analyzed using descriptive statistics, independent t-test, one-way analysis of variance, Scheffé test, Pearson's correlation coefficient analysis, and mediated model for PROCESS macro using the SPSS/WIN 24.0. Results: Self-directed learning was positively associated with self-determination (r=.56, p<.001) and academic self-efficacy (r=.63, p<.001). Furthermore, academic self-efficacy had a mediating effect on the relationship between self-determination and self-directed learning (B=0.21, 95% CI=0.12~0.32). Conclusion: The impact of self-determination on the self-directed learning among nursing students was mediated by academic self-efficacy. Therefore, these results provide important data for future self-directed learning in nursing education.

• Korean Journal of Veterinary Research
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• v.38 no.3
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• pp.559-564
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• 1998

The polymerase chain reaction(PCR) assay was used for rapid diagnosis from blood and organ samples experimentally infected with Listeria monocytogenes. This method used a pair of primers based on a unique region in the 16S rRNA sequence of L monocytogenes. Procedure A was based on dilution of the blood sample followed by lysis of bacterial cell and direct analysis of the lysate with PCR. In artificially infected blood samples with L monocytogenes, it was possible to detect fewer than 40 cells per ml of blood. However, L monocytogenes was detected low rates on infected organs by the direct PCR. In procedure B, enrichment cultivation was used to increase numbers of bacteria before lysis and PCR. L monocytogenes was detected from 23 samples of 24 liver and spleen, respectively, and 18 samples of 24 blood were found to be positive by PCR on a subset of 72 organ samples, whereas L monocytogenes were detected on 63 organ samples in classical culture technique. It was required to analyze including enrichment steps were 6h and 18h on the procedure A and B, respectively.

• Proceedings of the Korea Water Resources Association Conference
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• 2023.05a
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• pp.264-264
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• 2023

물순환 과정에서의 증발산은 장기적인 관점에서의 수자원 계획 수립 시 중요한 요소이다. 증발산은 기온, 상대습도, 일사량 등 기상학적 인자뿐만 아니라 증발표면, 식생분포 등 다양한 인자의 복합작용에 의해 일어나므로, 유역 단위에서 발생한 실제증발산(Actual evapotranspiration, AET)을 측정하기에는 기술적인 한계가 존재한다. 그러나 증발산 보완관계(Complementary relationship of evapotranspiration, CRE) 가설을 활용하면, 수문요소의 상호작용을 고려한 모델링을 거치지 않고도, 비교적 간단하게 AET를 추정할 수 있다. 본 연구는 증발산 관측자료를 기반으로 유역 단위에서의 CRE를 검증하고자 하며, 플럭스 타워 등 다양한 관측장비가 설치되어 있는 용담댐 시험유역을 대상유역으로 선정하였다. 용담댐 유역 내 산지에 위치한 덕유산 플럭스 타워에서 측정된 증발산을 AET로 보았으며, 유역 인근에 위치한 전주 기상관측소에서 측정되는 팬 증발량(E_pan)을 잠재증발산량(Potential evapotranspiration, PET)으로 보았다. E_pan 계측시, 증발팬의 가열 등 주변환경 변화로 인해 과다하게 추정되는 값을 보완하기 위해 FAO Penman-Monteith 식을 활용해 팬 증발량 보정계수(Coefficient of pan evaporation, k_p)를 산정하여 적용하였다. 습윤증발산량(Wet evapotranspiration, WET)은 대기가 완전히 포화되었을 때 발생하는 증발산량으로, 댐 수표면에서 계측되는 수면증발량을 WET로 보았다. CRE 검증을 위해 AET와 PET를 각각 WET로 나누어 AET+와 PET+로 무차원화하였으며, 습윤지수(Moisture Index, MI)는 AET를 PET로 나누어 산정하였다. CRE 가설은 MI에 따른 AET+와 PET+가 서로 보완관계를 갖는다는 것인데, 용담댐 유역의 관측자료를 활용하여 CRE를 검증한 결과 AET+와 PET+ 간의 비대칭계수(b)가 1.23인 것으로 나타났다. 이 때의 평균제곱오차(MSE)는 0.599, 결정계수(R₂)는 0.631로 나타나 CRE의 b가 적합하게 추정된 것으로 판단된다. 본 연구결과와 같이 검증된 CRE를 통해 증발산 관측지점이 없거나, 조밀하지 않은 유역의 AET를 간접추정할 수 있으며, 이를 활용해 보다 정확한 댐의 장기유출 모의와 용수공급계획 수립에 도움을 줄 수 있을 것으로 기대된다.