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Tolerance of Korean Cronobacter spp. (Enterobacter sakazakii) Isolates to Dessication (국내에서 분리한 Cronobacter spp.(Enterobacter sakazakii)의 건조내성 특성)

  • Lee, Eun-Jin;Ryu, Tae-Hwa;Park, Jong-Hyun
    • Korean Journal of Food Science and Technology
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    • v.41 no.6
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    • pp.681-686
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    • 2009
  • Cronobacter spp. (Enterobacter sakazakii) is known to be highly resistant to dry conditions than any other Enterobacteriaeae. In this study, one hundred and ten Korean Cronobacter isolates were characterized to find out their survival characteristics under conditions of desiccation and humidity. Thirty percentage strains of the isolates showed high resistance to desiccation exposed on the metal surface for eight hours by half survival of the initial number, whileas less than 10% strains showed dry sensitivity by less one log scale survival among seven log scales. Finally, more than 90% of the strains consisted of dry-resistant and dry-intermediate groups. The same tendencies were evident in a 15-day exposure. Dry-resistant and intermediate strain groups showed 3 log scale survival among 5 log initial numbers in the powdered infant formula for 30 days, which were more resistant than on the above metal surface exposed. So, almost the isolate strains showed high resistance to dry condition. Dry-resistant and intermediate groups exposed on the metal surface formed a biofilm at the beginning, and the dry-sensitive group showed biofilm formation mainly only after a 7-day exposure. However, without a time difference in formation of biofilm, the dry-resistant and sensitive isolates seemed to similar biofilm formation activity. Most of the isolates showed very low survival at 75% relative humidity in 48 hours; however, they showed high resistance by 60% survival at 40% relative humidity. The Cronobacter isolates showed high resistance to desiccation on the metal surface and in the powdered infant formula, but low survival at high relative humidity. Therefore, high humidity may be a control method for Cronobacter in food processing environments.

A Study on Significance Testing of Driver's Visual Behavior due to the VMS Message Display Forms on the Road (도로상 VMS 표출방식별 운전자 유의성 검증에 관한 연구)

  • Kum, Ki-Jung;Son, Young-Tae;Bae, Deok-Mo;Son, Seung-Neo
    • International Journal of Highway Engineering
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    • v.7 no.4 s.26
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    • pp.151-162
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    • 2005
  • Variable Message Sign (VMS), which provides drivers with direct information about state of traffic congestion and for prevent an accident, is the most effective method among the methods of providing information in Advanced Transportation Management System. Currently establishment and the VMS which is operated foundation lets in Guidelines on the use of Variable message sign (a book of the VMS) of 1999 November the Ministry Construction & Transportation, these contents mean main viewpoint on physical part such as message special quality variable (font, character size and line space, word interval) and position mainly among standard about establishment in general. But, it is true that using without effect verification on the character of VMS display and that using mode of stationary-centered. In this paper, it executed significance test to effort verification on the character of VMS display for more practical and effective information transmission based on the driver viewpoint For the researches; develop 3D-Simulation, select characteristics of driver's visual cognition behavior (the conspicuity, the legibility and the comprehensibility), evaluation each issue (day or night, 80km/h or 100km/h). Especially, that used the Eye Marker Recorder to measure of reading-time (legibility) thus, confirmed objectivity and reduce an observational error. The results showed that the conspicuity is Flashing> Stationary>Scroll. The legibility is not deference that Flashing between stationary form. Also the comprehensibility result showed that Flashing> Stationary>Stroll form.

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Developmental Standard of the Short Sensory Profile for Korean Children of School Age (7 to 9 years old) (만 7~9세 학령기아동의 감각통합 임상관찰평가의 발달기준에 관한 일연구)

  • Ji, Seok-Yeon;Kim, Mi-Sun;Keum, Hyo-Jin;Kim, Sung-Hee
    • The Journal of Korean Academy of Sensory Integration
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    • v.7 no.1
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    • pp.27-36
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    • 2009
  • Introduction : Occupational therapists commonly use clinical observation to assess neuromuscular function witch is a fundamental component of sensory integration function. Clinical Observation of Motor and Postural Skills (COMPS) is a standardized assessment with seven items and used to screen if a child's problem is due to neuromuscular and sensory integration system. However, developmental standard of the test need to be validated with Korean children. Objective : This study is purposed to propose developmental standard of the COMPS for Korean children. Method : Seven to nine years old students (76 male and 70 female) participated in this study. In order to find out any difference by gender and age, the data was analyzed using t-test and ANOVA. Results : There is no significant difference by gender for all other items except Prone Extension Position (PEP). There is significant difference between children who are 7 years old and those who are 9 years old for Slow Motion(SM), Finger-Nose Touching (FNT), Asymmetrical Tonic Neck Reflex (ATNR), Supine Flexion(SF). There is also significant difference between those who are 8 years old and 9 years old for SM, FNT, ATNR. However, there is no significant difference between those who are 7 years and 8 years old. Conclusions : This study examines any difference in neuromuscular characteristics by age among school-aged children, based on the COMPS. The result of this study will provide a good evidence to establish developmental standard of COMPS for Korean children. It issuggested to continue further standardization work of the COMPS in order to establish a developmental standard for Korean children.

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Influence of Cerebral Protection Methods in Thoracic Aortic Surgery Using Hypothermic Circulatory Arrest (저체온 순환정지를 이용한 흉부 대동맥 수술 시 뇌관류 방법에 따른 수술결과)

  • Kim, Jae-Hyun;Na, Chan-Young;Oh, Sam-Sae
    • Journal of Chest Surgery
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    • v.41 no.2
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    • pp.229-238
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    • 2008
  • Background: Protection of the brain is a major concern during thoracic aortic surgery using hypothermic circulatory arrest (HCA). This study compares the surgical outcomes of two different cerebral protection methods in thoracic aortic surgery using HCA: retrograde cerebral protection (RCP) and antegrade cerebral protection (ACP). Material and Method: We retrospectively reviewed data on 146 patients who underwent thoracic aortic surgery from May 1995 to February 2007 using either RCP (114 patients, Group 1) or ACP (32 patients, Group 2) during HCA. There were 104 dissections (94 acute and 10 chronic) and 42 aneurysms (41 true aneurysms and 1 pseudoaneurysm), and all patients underwent ascending aortic replacement. There were 33 cases of hemiarch replacement, 5 of partial arch replacement, and 21 of total arch replacement. Result: The two groups were similar in preoperative and operative characteristics, but Group 2 had more elderly (over 70 years old) patients (34.4% vs. 10.5%), more coronary artery diseases (18.8% vs. 4.4%), more total arch replacements (46.9% vs. 5.3%) and longer HCA time ($50{\pm}24$ minutes vs. $32{\pm}17$ minutes) than Group 1. The operative mortality was 4.4% (5/114) and 3.1% (1/32), the incidence of permanent neurologic deficits was 5.3% (6/114) and 3.1% (1/32), and the incidence of temporary neurologic deficits was 1.8% (2/114) and 9.4% (3/32) in Groups 1 and 2, respectively. There were no statistical differences between the two groups in operative mortality, postoperative bleeding, or neurologic deficits (permanent and temporary). Conclusion: The early outcomes of aortic surgery using HCA were favorable and showed no statistical difference between RCP and ACP. However, the ACP patients endured longer HCA times and more extended arch surgeries. ACP is the preferred brain protection technique when longer HCA time is expected or extended arch replacement is needed.

Embryonic Development and Early Life History of the Northern Loach, Cobitis pacifica (Pisces: Cobitidae) (북방종개 Cobitis pacifica(Pisces: Cobitidae)의 배 발생과 초기생활사)

  • Lee, Wan-Ok;Kim, Kyeong-Hwan;Baek, Jae-Min;Kang, Young-Jin;Jeon, Hyoung-Zoo;Kim, Chi-Hong
    • Korean Journal of Ecology and Environment
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    • v.44 no.1
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    • pp.1-8
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    • 2011
  • We investigated developmental stages of embryo and early life history of the Korean indigenous fish, the northern loach, Cobitis pacifica in 2009 in order to understand fundamental knowledges for conservation of this species. Eggs were obtained after hormones injections (LHRH-a, HCG) and were artificially fertilized by the dry method. The embryo was spherical, separative demersal, faint white, and averaged $1.09{\pm}0.04\;mm$ (n=20) in diameter. The hatching of the embryo took place in about 48 hours after fertilization under water temperature of $21.0{\sim}24.0^{\circ}C$ and the newly hatched larvae averaged $2.87{\pm}0.05\;mm$ (n=20) in total length (TL). Four days after hatching, the larvae grew up to $6.86{\pm}0.10\;mm$ (n=10) in TL and york sac absorption, mouth and anus opening were shown. Fourteen days after hatching, most of fin-rays appeared at $10.71{\pm}0.34\;mm$ (n=10) in TL and color spots on the body surface were attained. Twenty six days after hatching, the larvae grew up to $14.88{\pm}0.45\;mm$ (n=10) in TL, and all their fin-rays were formed. Therefore, according to current study regarding the morphological development of Cobitis pacidica, the conversion from larval to juvenile stages occurred at 26 days after hatching. Eighty days after hatching, the larvae were $33.3{\pm}1.25\;mm$ (n=10), and their body shape and color pattern were similar to adult fish. In this study, embryonic development and early life history of the northern loach, Cobitis pacifica show morphological characteristics of Cobitidae family. We expected that our results can be used as an fundamental knowledges for restoration study of indigenous fish species.

Agroclimatology of North Korea for Paddy Rice Cultivation: Preliminary Results from a Simulation Experiment (생육모의에 의한 북한지방 시ㆍ군별 벼 재배기후 예비분석)

  • Yun Jin-Il;Lee Kwang-Hoe
    • Korean Journal of Agricultural and Forest Meteorology
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    • v.2 no.2
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    • pp.47-61
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    • 2000
  • Agroclimatic zoning was done for paddy rice culture in North Korea based on a simulation experiment. Daily weather data for the experiment were generated by 3 steps consisting of spatial interpolation based on topoclimatological relationships, zonal summarization of grid cell values, and conversion of monthly climate data to daily weather data. Regression models for monthly climatological temperature estimation were derived from a statistical procedure using monthly averages of 51 standard weather stations in South and North Korea (1981-1994) and their spatial variables such as latitude, altitude, distance from the coast, sloping angle, and aspect-dependent field of view (openness). Selected models (0.4 to 1.6$^{\circ}C$ RMSE) were applied to the generation of monthly temperature surface over the entire North Korean territory on 1 km$\times$l km grid spacing. Monthly precipitation data were prepared by a procedure described in Yun (2000). Solar radiation data for 27 North Korean stations were reproduced by applying a relationship found in South Korea ([Solar Radiation, MJ m$^{-2}$ day$^{-1}$ ] =0.344 + 0.4756 [Extraterrestrial Solar Irradiance) + 0.0299 [Openness toward south, 0 - 255) - 1.307 [Cloud amount, 0 - 10) - 0.01 [Relative humidity, %), $r^2$=0.92, RMSE = 0.95 ). Monthly solar irradiance data of 27 points calculated from the reproduced data set were converted to 1 km$\times$1 km grid data by inverse distance weighted interpolation. The grid cell values of monthly temperature, solar radiation, and precipitation were summed up to represent corresponding county, which will serve as a land unit for the growth simulation. Finally, we randomly generated daily maximum and minimum temperature, solar irradiance and precipitation data for 30 years from the monthly climatic data for each county based on a statistical method suggested by Pickering et a1. (1994). CERES-rice, a rice growth simulation model, was tuned to accommodate agronomic characteristics of major North Korean cultivars based on observed phenological and yield data at two sites in South Korea during 1995~1998. Daily weather data were fed into the model to simulate the crop status at 183 counties in North Korea for 30 years. Results were analyzed with respect to spatial and temporal variation in yield and maturity, and used to score the suitability of the county for paddy rice culture.

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The Factors Associated with Dental Caries Experience and Oral Hygiens Status in Smoking Adolescents (흡연청소년의 치아우식경험도 및 구강위생 관련요인)

  • Shin, Seon-Haeng;Kim, Myung-Seok
    • Journal of dental hygiene science
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    • v.9 no.5
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    • pp.497-506
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    • 2009
  • This study was conducted to estimate the dental caries experience, oral hygiene status and the factors influencing the dental disease in the smoking adolescents and to provide the baseline data for managing smokers efficiently. We recruited 156 smokers(male: 106, female: 50) in middle, high school students in 5 day Non-smoking program in seoul city and 176 non-smokers(male: 64, female: 112) by matching method for considering sex and age from June 1 to August 31 2009. Data on general characteristics, basic oral health care, smoking factors, self-efficiency, control of oral health, oral health promotion behavior, knowledge of oral health were collected by a questionnaire interview. DMFT index, DT index, MT index, FT index, Plaque index, Calculus index were calculated by the oral examination. The results of this study were as follows. 1. Dental clinic visit(p < 0.05), self-perception of oral health status(p < 0.001), oral health concern (p < 0.01) in non-smoker group were significantly higher than that of smoker group. 2. self-efficiency(p<0.05), oral health promotion behavior(p < 0.05) in non-smoker group were significantly higher than that of smoker group. 3. DT index, Plaque index, Calculus index in non-smoker group was significantly lower than that of smoker group(p < 0.0001). 4. The fewer smoke amount, the lower DT index(p < 0.05), Plaque index(p < 0.01), Calculus index(p < 0.001). 5. It was significant correlated among DT index and self-efficiency, oral health promotion behavior, control of oral health. 6. In multiple regression analysis, oral health promotion behavior, Plaque index was proved as a significant factors related with the degree of dental caries experience in smoking adolescents. In other word, the higher oral health promotion behavior, the lower Plaque index, the fewer DT index.

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Impact of Mother's Oral Health Literacy on Preschool Children's Oral Health Status and Behavior (어머니의 구강건강정보이해력이 유아 자녀의 구강건강상태와 행동에 미치는 영향)

  • Kang, Yu-Min;Cho, Young-Sik
    • Journal of dental hygiene science
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    • v.16 no.1
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    • pp.26-36
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    • 2016
  • The purpose of this study was to examine how oral health literacy of mothers affect the oral health status of their preschool children. The subjects were 233 mothers and their preschool children who are between 5 and 6 years old. They were selected according to the convenience sampling method. The individual self-administered questionnaire was used for the mother's survey while the children were interviewed using structured questionnaire to examine their oral health status and behavior. According to study results, the number of decayed primary teeth and the number of decayed and filled primary teeth had a statistically significant negative correlation with the oral health literacy of the mother, the children's oral health knowledge, attitude and behavior (COHKAB), and the mother's oral health management behavior. Hierarchical multiple regression analysis was performed after including general characteristics variables, the COHKAB and the mother's oral health management behavior. Meanwhile, mother's oral health literacy had a statistically influence on children's oral health status. The higher the mother's oral health literacy level, the lower the number of decayed and filled primary teeth were. The findings suggest that efforts to improve the oral health status of preschool children should consider mother's oral health literacy as an important factor. Therefore, the effective intervention and education programs are necessary to enhance mother's oral health literacy.

Fermentation Conditions for High Acceptability of Korean Traditional Fermented Beverage Kyejang (전통 발효음청류 계장의 기호도 우수 발효조건)

  • Jung, Jin-Kyoung;Song, Kyung-Mo;Yi, Sung-Hoon;Kim, Hyo-Jin;Han, Young-Sook;Lee, Myung-Ki
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.44 no.1
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    • pp.137-144
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    • 2015
  • Kyejang is a Korean traditional beverage manufactured from medicinal plants and fruits using honey, sugar, or starch in cold water. In this study, cinnamon-containing kyejang, which is a type of beverage Jang, was reproduced based on Imwonsibyukji's method published in 1827 in the Korean literature. Kyejang made by nuruk, cinnamon, and medicinal plants was prepared at various temperatures and periods. Kyejang was assayed for physiochemical properties (pH and acidity), contents of metabolites (organic acids, sugars, and amino acids), and sensory characteristics (aroma and taste). During fermentation, content of organic acids (e.g. lactic acid, acetic acid, and shikimic acid) increased, which lowered pH, increased acidity, and increased intensity of sour taste. In the case of free sugars, fructose and maltose levels decreased while glucose and mannitol levels increased during fermentation periods, and sweetness decreased. The main amino acid in kyejang was tryptophan, followed by asparagine, proline, and arginine. The sensory evaluation score of overall preference was highest for kyejang which was fermented at $20^{\circ}C$ for 3 days. The results will be provide the basic data of fermentation conditions for standardized manufacturing process of kyejang.

Studies on the utilization of sandy barren lands and sandy farm lands of low productivity -1. Studies on growing rice-plant in sandy barren lands (식량증산을 위한 유휴사지(遊休砂地) 및 사질계(砂質系) 농지(農地) 활용(活用)에 관한 기초적(基礎的) 연구(硏究) -1. 수도(水稻)의 사지재배(砂地栽培)에 관한 연구(硏究))

  • Kim, Yong Chul;Choe, Gyu Ho
    • Korean Journal of Soil Science and Fertilizer
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    • v.9 no.1
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    • pp.33-38
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    • 1976
  • As a basic studies for increasing food production utilizing sandy barren lands and sandy farmlands of low productivity which distributed widely in Korea, an experiment of growing rice-plant on sandy barren land was undertaken as follows. 1. Variety, IR-667 was adopted and the growing method was a nutrient-irrigation culture which aimed to minimize percolation loss in sand with an automatic contineous supplying nutrient solution for supplmenting the sand characteristics. 2. The growth type price-plant after heading was a typical higher yield plant, that is, numerous, small, narrow, and thickend leaves, straight attitute, dense fasciculated etc. though the rooting of plant after planting was delayed because of using paddy-field grown seedling. 3. The adaptability of rice-plants on sandy land seemed to be different by varieties and IR-667 was more adaptable than ordinary Japonica varieties. 4. Even at the period of heading and maturing, the root system of rice-plant grown on sand showed vigorous growth having more activated apical portions. while, even the lower leaves showed flourished state. 5. The suppling of calcium and magnecium in addition to nitrogen, phosphorus and potassium on sand made notable increase of stem number per plant, grain number per stem and yields.

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