• Biomolecules & Therapeutics
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• 제20권2호
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• pp.234-238
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• 2012

Propofol is an anesthetic commonly used to provide sedation or to induce and maintain an anesthetic stated. However, there are reports which indicate propofol may cause psychological dependence or be abused. In the present study, we used various behavioral tests including climbing test, jumping test, conditioned place preference, and self-administration test to assess the dependence potential and abuse liability of propofol compared to a positive control (methamphetamine) or a negative control (saline or intralipid). Among the tests, the conditioned place preference test was conducted with a biased method, and the selfadministration test was performed under a fixed ratio (FR) 1 schedule, 1 h per session. No difference was found in the climbing test and jumping test, but propofol (30 mg/kg, i.p.) increased the rewarding effect in the conditioned place preference test, and it showed a positive reinforcing effect compared to the vehicle. These results indicate that propofol tends to show psychological dependence rather than physical dependence, and it seems not to be related with dopaminergic system.

• 대한한의학회지
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• 제18권1호
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• pp.326-336
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• 1997

In order to investigate the effect of aqua-acupuncture solution with Panax Ginseng into $Qihai(CV_6)$ and $Shenshu(BL_{23})$ on immune response induced by glucocorticoid in mouse, Panax Ginseng aqua-acupuncture solution was injected into $Qihai(CV_6)$ and $Shenshu(BL_{23})$ for seven days after injection with glucocorticoid. And then MA-HRP (methamphetamine-horseradish peroxidase) induced antibody production, numbers and lysozyme activity in macrophage were measured. The results were as follows: 1. The antibody production in mouse immunized with MA-HRP was decreased in control group as compared with normal group. Although $Qihai(CV_6)$ group showed slight increasement, $Shenshu(BL_{23})$ group indicated great increasement compared with normal group. However in Aa-BL group, antibody production was almost increased to normal group. 2. In control group, the numbers of macrophage were decreased about 14% as compared with normal group. And in the pretreated groups of $Qihai(CV_6)$ and $Shenshu(BL_{23})$ were respectively increased 3.0 times and 2.9 times as compared with normal group. 3. Effect of Panax Ginseng-aqua acupuncture solution on the lysozyme activity in macrophage was increased gradually in the pretreated groups of $Qihai(CV_6)$ and $Shenshu(BL_{23})$ as compared with control group. These results suggest that Panax Ginseng aqua-acupuncture at $Qihai(CV_6)$ and $Shenshu(BL_{23})$ may increase antibody production and lysozyme activity in macrophage that is suppressed by glucocorticoid, and Panax Ginseng aqua-acupuncture will have immuno adjuvant effects on the cells which concerned with immunomechanisms.

• 약학회지
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• 제55권2호
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• pp.145-153
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• 2011

A simultaneous detection and quantification method for determining the Phenylalkylamine derivatives, such as methamphetamine (MA), amphetamine (AM), 3,4-methylenedioxymethamphetamine (MDMA), 3,4-methylenedioxyamphetamine (MDA), ketamine (KT), norketamine (NKT), phentermine (PT), fenfluramine (FFA) and phenmetrazine (PM), in oral fluid was developed and validated according to international guidelines. The validated method was applied to actual oral fluid samples collected from drug abuse suspects. The recovery of phenylalkylamines from oral fluid collection devices was also assessed. Oral fluid specimens from 20 drug abuse suspects submitted by the police were collected using Salivette$^{TM}$, Quantisal$^{TM}$ or direct expectoration. The samples were screened using a biochip array analyzer. For confirmation, the samples were analyzed by GC-MS in selected-ion monitoring (SIM) mode after extraction using automated SPE with a mixed-mode cation exchange cartridge and derivatization with trifluoroacetic anhydride (TFAA). The results from the immunoassay were consistent with those from GC-MS. All the oral fluid samples gave positive results for MA, AM, PT and/or PM. The detection of phenylalkylamines in oral fluid can provide a better indication of recent use than urine or hair. Therefore, the oral fluid specimen was useful for demonstrating phenylalkylamines abuse in the driving under the influence of drug (DUID) as an alternative specimen for urine.

• 고려인삼학회:학술대회논문집
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• 고려인삼학회 1998년도 Advances in Ginseng Research - Proceedings of the 7th International Symposium on Ginseng -
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• pp.168-173
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• 1998

A single administration of cocaine (CO), morphine (MOR) and methamphetamine (MA) showed hyperactivity in mice. Ginseng total saponin (GTS), ginsenosides Rbl and Rgl inhibited the hyperactivity induced by the drugs. The repeated administration of CO, MOR and MA showed the development of psychological dependence showing a.: the development of conditioned place preference (CPP) in mice and the development of dopamine (DA) receptor supersensitivity showing as sensitization of the drugs. GTS and Rgl inhibited the development of not only psychological dependence but also of DA receptor supersensitivity induced by CO and MA Rbl prevented also the development of psychological dependence and DA receptor supersensitivity induced by CO and MA but not by MOR. These results suggest that the development psychological dependence induced by the drugs is closely related with the development of DA receptor supersensitivity since both phenomena were inhibited by them. Apomorphine induced climbing behavior was also inhibited by G75 but not by both of Rbl and Rgl, indicating that GTS modulate dopaminergic action at both of pre and postsynaptic sites, but both of Rbl and Rgl , only at the presynaptic site. These results suggest that active components acting at the postsynaptic site exist in GTS. In this study, it was found that GTS, ginsenosides Rbl and Rgl inhibited tyrosine hydroxylase (TH) and these components exerted inhibitory effects on both Cal' currents and $\Delta$ Cm in rat adrenal chromaffin cells. These results suggest that G75 and ginsenosides regulate catecholamine synthesis and secretion. Meanwhile, it has been demonstrated that Rbl, at high doses has more powerful inhibition of cartecholamine secretion at the presynaptic site than Rbl. Therefore, it was presumed that inhibition of morphine induced psychological dependence by Rgl, but not by Rbl results from differences in the extent of this inhibitory action on dopaminergic synthesis and secretion.

• 한국학교보건학회지
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• 제8권2호
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• pp.257-275
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• 1995

The purpose of this study was to provide basic data for preventing the youth from their dug abuse and contributing to improve their health. A total of 1,210 students of middle and high schools in Pusan were selected to answer this questionnaire. The data was collected from March 23 to April 6, 1995. The major findings of this study are as follows; 1) The attitude about drugs of middle and high school students was desirable. By groups, girl students showed more desirable attitude than boy students, and middle school students than high school students. 2) For the experience of drinking coffee, 70.4% of the subjects of survey answered that they have had:62.4% of middle school students and 78.9% of high school students. For the stated time of drinking coffee 59.8% of them, the largest number, said from middle school days. 3) For the experience of drinking alcoholic liquors, 40.4% of the subjects of survey answered that they have had: 51.4%n of boy students and 29.5% of girl students, 23.4% of middle school students and 57.7% of high school students. For the started time of drinking liquors 53.1% of them, the largest number, said from middle school days. 4) For the experience of smoking, 24.8% of the subjects of survey answered that they have had: 37.2% of boy students and 12.5% of girl students, 16.3% of middle school students and 33.3% of high school students. For the started time of smoking 52.0% of them, the largest number, said from middle school days. 5) For the experience of drugs, 6.3% of the subjects of survey answered that they have had. For the started time of abusing drugs 71.0% of them, the largest number, said from middle school days. The kinds and composing ratio of abused drugs were: Timing was 51.4%, bond 20.3%, butane gas 12.6%, thinner 3.8%, methamphetamine 1.9% and mescaline 1.9%. 6) The attitude points toward drugs in case of not taking coffee and liquors, smoking and abusing drugs were higher than the points in case of taking them. 7) The rate of abusing drugs in case of taking coffee and liquors and smoking was higher than that in case of not taking them and smoking.

• Toxicological Research
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• 제11권2호
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• pp.315-327
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• 1995

Diverse neurotoxic insults result in proliferation and hypertrophy of astrocytes, a subtype of glia in central nervous system. The hallmark of this response, often terms "reactive gliosis", is the enhanced expression of the major intermediate filament protein of castrocytes, glial fibrillary acidic protein (GFAP). These changes in the astrocytes suggest that GFAP may be a useful biochemical indicator of neurotoxicity. To investigate this possibility, we administered intra-peritoneally prototype nerotoxicants, metharnphetamine (MAP, 5 mg/kg), cocaine (30 mg/kg), N-buthyl benzenesulfonamide (NBBS, 300 mg/kg) and trimethytin (TMT, 8 mg/kg) to Wistar Rats and then assessed the effects of these agents on content of GFAP, which were determined by Sandwish ELISA and evaluated with neurotoxic symptoms, and quantitative changes of imrnunoreactivity of GFAP by light microscopic image analysis in specific regions. We found that assay of GFAP revealed time- and region-dependant patterns of neurotoxicity. The GFAP immunoreactivity of rat brain was increased in substantia nigra and hippocampus by MAP, NBBS and TMT; in roedial septal nucleus and nucleus accurnbens, it was also increased by RrBBS. Sandwich ELISA showed that GFAP levels of cerebrum in all groups on days 3 and 7 and that of brainstem(including cerebellum) in MAP, NBBS groups on day 1 and 3 were increased. A review of the background, design and results of these experiments are presented in this paper. Our findings indicate that GFAP is a sensitive and specific biomarker of neurotoxicity.otoxicity.

• Biomolecules & Therapeutics
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• 제25권6호
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• pp.578-585
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• 2017

Recently, there has been a rise in the number of amphetamine derivatives that serve as substitutes for controlled substances (e.g. amphetamine and methamphetamine) on the global illegal drug market. These substances are capable of producing rewarding effects similar to their parent drug. In anticipation of the future rise of new and similar psychoactive substances, we designed and synthesized four novel amphetamine derivatives with N-benzyl, N-benzylamphetamine HCl (NBNA) substituent on the amine region, 1,4-dioxane ring, ethylenedioxy-amphetamine HCl (EDA), methyl, para-methylamphetamine HCl (PMEA), and naphthalene, 2-(aminopropyl) naphthalene HCl (2-APN) substituents on the phenyl site. Then, we evaluated their abuse potential in the conditioned place preference (CPP) test in mice and self-administration (SA) test in rats. We also investigated the psychostimulant properties of the novel drugs using the locomotor sensitization test in mice. Moreover, we performed qRT-PCR analyses to explore the effects of the novel drugs on the expression of D1 and D2 dopamine receptor genes in the striatum. NBNA, but not EDA, PMEA, and 2-APN, induced CPP and SA in rodents. None of the test drugs have produced locomotor sensitization. qRT-PCR analyses demonstrated that NBNA increased the expression of striatal D1 dopamine receptor genes. These data indicate that NBNA yields rewarding effects, suggesting potential for abuse. Continual observation for the rise of related substances is thus strongly encouraged.

• 디지털콘텐츠학회 논문지
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• 제18권8호
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• pp.1551-1560
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• 2017

본 논문의 목적은 가상현실을 활용한 중독치료의 동향 파악을 위해 중독의 종류와 물질사용장애에 대한 가상현실치료 방법을 분류하고 분석하는 것이다. 가상현실은 다양한 정신장애 및 심리문제를 치료하기 위한 새로운 패러다임으로 활용되어 왔다. 최근에는 많은 연구가 수행되어 중독치료, 특히 물질사용장애에 대한 가상현실치료의 효과를 입증하였다. 이러한 연구들의 경향을 파악하기 위하여, 이 논문은 학술문헌 데이터베이스인 ScienceDirect, MEDLINE, ProQuest 그리고 Embase에 등재된 논문들을 체계적으로 검토하였다. 그 결과 45편의 연구논문들이 검색되었는데, 이 연구들 중에서 니코틴/흡연에 관하여 가장 많은 연구 (73 %, n = 33)가 되었고, 알코올 (18 %, n = 8), 대마초/마리화나 (2 %, n = 1), 그리고 헤로인, 코카인, 메스암페타민 등 기타 약물(7 %, n = 3)에 대한 연구가 이루어졌다. 이 논문은 이러한 연구들의 결과를 분석하고, 장점뿐만 아니라 몇 가지 한계점을 발견했으며, 가상 현실기술을 이용한 중독치료에 대한 추가연구 방향을 제시하였다.

• 한국융합학회논문지
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• 제13권3호
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• pp.275-280
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• 2022

본 연구는 청소년기에서 성인기로 진입하는 청년층의 약물남용에 대한 인식과 태도를 살펴 보기위해 분석 가능한 설문지 1076부를 토대로 분석하였다. 설문에 참여한 청년들의 성별을 보면 남자 699명, 여자 377명이고 남용약물 중 필로폰, 대마, 본드, 프로포폴에 대한 인식이 높았다. 남용약물의 위험성에 대한 인식은 384명(35.7%)이 잘 알고 있는 것으로 나타났으나, 남용약물이 유발하는 다양한 문제에 대해서 잘 알고 있다가 249명(23.1%), 잘 모른다는 442명(41.1%)로 거의 2배 가까이 높게 나타났다. 약물남용에 대한 문제를 개선하기 위한 바람직한 방안은 강력한 처벌이 53.6%로 가장 높게 나왔으며 약물남용 예방을 위한 효과적인 교육 방법으로 학교 필수교육 53.5%로 나타났다. 따라서 약물남용 예방을 위한 홍보나 교육 사업이 좀 더 현실적으로 진행되어야 하며 청년들이 공감할 수 있는 예방 교육 프로그램을 개발되어야 한다. 또한 약물 남용에 의한 약물 중독은 거의 대부분이 재발될 수 있으므로 재활 대책 수립은 필수적이며 약물사용에 대한 올바른 교육은 지속적으로 필요하다고 사료된다.

• 분석과학
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• 제22권3호
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• pp.210-218
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• 2009

GC-MS/MS를 이용하여 모발 중 암페타민계 마약류 4종과 노르케타민의 동시 분석법을 확립하였으며 결과의 신뢰도를 높이기 위해 분석법 검정을 실시하였다. 모발 시료의 전처리 과정은 증류수와 아세톤을 이용하여 세척, 자동분쇄기를 이용한 세절, 메탄올 용매를 이용한 추출 및 배양 그리고 HFBA로 유도체화하는 단계를 거쳐 GC-MS/MS를 이용하여 분석하였다. 그 결과 모든 분석물질에 대해 정량범위 내에서 우수한 직선성을 나타내었으며, 실제 마약 복용자의 모발감정에 이용될 수 있는 충분한 감도와 선택성, 정밀도와 정확도를 확인하였다. 검정곡선의 결정계수($r^2$)는 0.998 이상을 나타내었고 검출 한계는 0.007 ng/mg 이하였으며 회수율은 75.9-100.9%이었다. 일내 (intra-day) 및 일간 (inter-day) 정확도는 -2.6-17.0%의 범위를 나타내었으며 정밀도는 10.7%이하로 모두 기준값(20% 이하)이내의 값을 나타내었다. 확립된 분석법을 마약 복용자의 모발에 적용한 결과 메스암페타민의 농도 분포는 0.97-19.30 ng/mg이었으며 암페타민은 0.14-2.56 ng/mg이었다.