YAKHAK HOEJI (약학회지)

The Pharmaceutical Society of Korea (대한약학회)
권호 표지

Development of Simultaneous Quantification Method of Phenylalkylamines in Oral Fluid

타액 중 페닐알킬아민유도체류의 일제분석법 확립

  • Choi, Hye-Young (Narcotics Analysis Division, National Institute of Scientific Investigation) ;
  • Baeck, Seung-Kyung (Narcotics Analysis Division, National Institute of Scientific Investigation) ;
  • Jang, Moon-Hee (Narcotics Analysis Division, National Institute of Scientific Investigation) ;
  • Choi, Hwa-Kyung (Narcotics Analysis Division, National Institute of Scientific Investigation) ;
  • Chung, Hee-Sun (Narcotics Analysis Division, National Institute of Scientific Investigation)
  • 최혜영 (국립과학수사연구원 마약분석과) ;
  • 백승경 (국립과학수사연구원 마약분석과) ;
  • 장문희 (국립과학수사연구원 마약분석과) ;
  • 최화경 (국립과학수사연구원 마약분석과) ;
  • 정희선 (국립과학수사연구원 마약분석과)
  • Received : 2011.02.15
  • Accepted : 2011.03.20
  • Published : 2011.04.30
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Abstract

A simultaneous detection and quantification method for determining the Phenylalkylamine derivatives, such as methamphetamine (MA), amphetamine (AM), 3,4-methylenedioxymethamphetamine (MDMA), 3,4-methylenedioxyamphetamine (MDA), ketamine (KT), norketamine (NKT), phentermine (PT), fenfluramine (FFA) and phenmetrazine (PM), in oral fluid was developed and validated according to international guidelines. The validated method was applied to actual oral fluid samples collected from drug abuse suspects. The recovery of phenylalkylamines from oral fluid collection devices was also assessed. Oral fluid specimens from 20 drug abuse suspects submitted by the police were collected using Salivette$^{TM}$, Quantisal$^{TM}$ or direct expectoration. The samples were screened using a biochip array analyzer. For confirmation, the samples were analyzed by GC-MS in selected-ion monitoring (SIM) mode after extraction using automated SPE with a mixed-mode cation exchange cartridge and derivatization with trifluoroacetic anhydride (TFAA). The results from the immunoassay were consistent with those from GC-MS. All the oral fluid samples gave positive results for MA, AM, PT and/or PM. The detection of phenylalkylamines in oral fluid can provide a better indication of recent use than urine or hair. Therefore, the oral fluid specimen was useful for demonstrating phenylalkylamines abuse in the driving under the influence of drug (DUID) as an alternative specimen for urine.

Keywords

References

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