• 한국자원식물학회지
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• 제34권6호
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• pp.510-516
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• 2021

Whitening agents derived from natural sources which do not have side effects are sought after. Schizophragma hydrangeoides is an edible plant that grows wild on Jeju Island. We aimed to determine whether S. hydrangeoides extracts show anti-melanogenic activity. Here, we found that 70% ethanol extracts of S. hydrangeoides leaf suppressed α-melanocyte-stimulating hormone-induced melanogenesis in B16F10 mouse melanoma cells. This activity of anti-melanogenesis in B16F10 cells were investigated by determining melanin content and tyrosinase activity, and by performing western blotting. The 70% ethanol extract downregulated tyrosinase and tyrosinase-related protein 1. In addition, the n-hexane fraction of S. hydrangeoides leaf (HFSH) exhibited significant anti-melanogenic activity among the various solvent fractions tested without reducing the viability of B16F10 cells. Taken together, these results indicate that extracts from S. hydrangeoides leaf can influence cellular processes via modulation of tyrosinase activity. Hence, S. hydrangeoides can be used as a whitening agent in the cosmetic industry and as a therapeutic agent for treating hyperpigmentation disorders in the clinic.

• 대한화장품학회:학술대회논문집
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• 대한화장품학회 2003년도 IFSCC Conference Proceeding Book II
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• pp.243-244
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• 2003

The effects of Lactic acid bacteria have been investigated on anti-tumor. cholesterol reduction in blood. promotion of immune and skin-beauty. We are focused on cosmeceutical activity of Lactic acid bacteria (LAB). Ml, which is found in Korean traditional food. Kimchi The LAB.Ml has been identified as Lactobacillus plantarum Ml and individually cultured with Soybean soup and Soybean-Curd whey, until the total acidity has been reached the highest. After then, cell-free extracts from Ml have been used for the following studies. We assessed the effect of Lactobacillus plantarum Ml on the depigmentation of B16FlO melanoma cell. The melanin content of cells was decreased with 1-3% of cultured extracts. The tyrosinase activity was reduced by cell-free extracts of Lactobacillus plantarum Ml. Anti-aging and anti-oxidative activity of Ml cultured extract was also studied in NIH-3T3 human fibroblast cells. It showed that induction of cell proliferation. collagen synthesis and free radical scavenging activity. Additional studies for anti-fungal and anti-acne activity were also detected on Staphylococcus aureus and Propionibacterium acnes, respectively. These results suggest that cultured extract of Lactobacillun plantarum Ml would be used for cosmeceutical ingredients through multifunctional reaction on skin such as whitening, anti-wrinkle. anti-oxidation and anti-acnes.

• 한국임상수의학회지
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• 제40권5호
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• pp.370-374
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• 2023

Oral melanoma is the most common type of oral tumor in dogs. In this report, computed tomography (CT) and magnetic resonance imaging (MRI) were performed to diagnose a right oral pigmented mass in an 8-year-old dog. The oral mass appeared as a homogeneous soft tissue density parenchyma on pre-contrast CT images, and with heterogeneous enhancement on post-contrast images. Bone destruction of the right mandibular body around the mass and mild enlargement of the right mandibular lymph node were also found. On MRI, the bulky oral mass showed mixed hyperintensity and isointensity compared to the adjacent muscle, where irregular hyperintensity on T1-weighted images corresponded to hypointensity on the T2-weighted images. Based on the physical examinations and imaging results, melanoma was suspected and confirmed via fine-needle aspiration. These unique MRI signals were due to the high paramagnetic melanin content in the tumor, therefore MRI examination could be useful for diagnosis of melanoma.

• Toxicological Research
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• 제33권1호
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• pp.55-62
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• 2017

We evaluated the antioxidant activity and anti-melanogenic effects of Oenothera laciniata methanol extract (OLME) in vitro by using melan-a cells. The total polyphenol and flavonoid content of OLME was 66.3 and 19.0 mg/g, respectively. The electron-donating ability, 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical-scavenging activity, and superoxide dismutase (SOD)-like activity of OLME ($500{\mu}g/mL$) were 94.5%, 95.6%, and 63.6%, respectively. OLME and arbutin treatment at $50{\mu}g/mL$ significantly decreased melanin content by 35.5% and 14.2%, respectively, compared to control (p < 0.05). OLME and arbutin treatment at $50{\mu}g/mL$ significantly inhibited intra-cellular tyrosinase activity by 22.6% and 12.6%, respectively, compared to control (p < 0.05). OLME ($50{\mu}g/mL$) significantly decreased tyrosinase, tyrosinase-related protein-1 (TRP-1), TRP-2, and microphthalmia-associated transcription factor-M (MITF-M) mRNA expression by 57.1%, 67.3%, 99.0%, and 77.0%, respectively, compared to control (p < 0.05). Arbutin ($50{\mu}g/mL$) significantly decreased tyrosinase, TRP-1, and TRP-2 mRNA expression by 24.2%, 42.9%, and 48.5%, respectively, compared to control (p < 0.05). However, arbutin ($50{\mu}g/mL$) did not affect MITF-M mRNA expression. Taken together, OLME showed a good antioxidant activity and anti-melanogenic effect in melan-a cells that was superior to that of arbutin, a well-known skin-whitening agent. The potential mechanism underlying the anti-melanogenic effect of OLME was inhibition of tyrosinase activity and down-regulation of tyrosinase, TRP-1, TRP-2, and MITF-M mRNA expression.

• 대한한의학방제학회지
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• 제25권3호
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• pp.337-347
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• 2017

Objectives : Atopic Dermatitis (AD) is chronic skin disease characterized by allergic hypersensitivity reactions. Samhwangsasim-tang Gamibang(SHSST) can treat skin disease by cooling down blood heat, clearing away congenital fever and detoxicating. This study was conducted to investigate the effects of SHSST on AD induced by oxazolone in mice. Methods : We investigated the effects of SHSST on skin tickness, skin lesions, erythema and melanin index, water content and histopathological changes in vivo. The effects on body weights and spleen body weight ratio were also investigated in mice with AD. Results : In our results, oral administration of SHSST ameliorated skin lesion such as flare, erythema, exudate and petechia and lowered skin thickness induced by oxazolone. In addition, SHSST lowered erythema index and elevated water content compared to AD control respectively. In the histopathological observation, oral administration of SHSST prevented epidermal hyperplasia and spongiotic changes which is a hallmark of skin inflammation respectively. Finally, SHSST did not affect spleen/body weight ratio. Conclusions : These data imply that SHSST can ameliorate skin lesion of AD such as flare, erythema, exudate and petechia, and be used to treat AD patients with relative safety.

• 한국응용과학기술학회지
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• 제34권3호
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• pp.602-612
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• 2017

본 연구에서는 잣나무 잎으로 70% 에탄올 추출물과 증류수 추출물을 얻은 후 항산화 효과, 항염증, 미백효과 등 다양한 효과를 검증하고자 연구를 수행하였다. 항산화 실험 결과, 폴리페놀, 플라보노이드 효과에서는 폴리페놀과 플라보노이드 함량이 농도 의존적으로 증가하였다. 폴리페놀과 플라보노이드 함량은 증류수 추출물에 비해 에탄올 추출물에서 높은 것으로 확인되었다. B16F10, RAW264.7 세포에서 농도별로 세포독성이 나타나지 않았으며, NO 생성 억제능 측정 결과, LPS로 유도된 NO 생성을 효과적으로 억제하여 항염증 활성이 있을 것으로 예측되었다. 멜라닌 생합성 억제능 측정결과, 유의한 감소효과를 확인하였으며, western blot을 수행하여 MITF, Tyrosinase 단백질 발현억제 실험결과, 농도 의존적으로 현저히 억제됨을 확인할 수 있었다. 따라서 본 연구결과로부터 잣나무 잎추출물은 화장품 소재로서의 다양한 활용 가능성이 있을 것으로 사료된다.

• 대한화장품학회지
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• 제46권2호
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• pp.159-166
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• 2020

프로폴리스는 식물에서 채취한 수지에 꿀벌의 분비물이 합쳐져 만들어진 아교성 물질로 세균이나 바이러스로부터 자신을 보호하는 기능을 한다. 본 연구에서는 한국, 중국, 브라질 유래 프로폴리스 추출물의 항산화, 항균, 미백, 항염 활성을 비교하고, 이들 추출물의 화장품소재로서의 응용가능성을 살펴보았다. 플라보노이드, 폴리페놀함량분석과 자유라디칼 소거능 시험을 통해 항산화 활성을 확인한 결과 한국, 중국, 브라질 프로폴리스 추출물 모두 유의한 항산화 효능을 보였다. 피부에 상재하는 미생물에 대한 항균효능을 MIC 시험법을 통해 측정한 결과 C. acnes 균에서 한국 프로폴리스 추출물이 다른 추출물에 비해 우수한 항균력을 보였다(KPE: 62.5 ㎍/mL, CPE: 250 ㎍/mL, BPE: 500 ㎍/mL). 또한, 한국 프로폴리스 추출물은 멜라닌 세포의 멜라닌 생성을 억제하였고, 마우스대식세포에서 리포폴리사카라이드로 유도된 염증인자인 산화질소와 PGE₂ 생성을 나머지 두 추출물보다 우수하게 억제하였다. 이들 결과를 종합하면 프로폴리스 추출물은 항산화, 항균, 항염 효능소재로 응용될 수 있으며, 특히 항균, 항염, 미백에서 우수한 효능을 보인 한국프로폴리스추출물의 응용가능성이 우수함을 확인할 수 있었다.

• 생명과학회지
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• 제25권3호
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• pp.269-275
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• 2015

생체에서 가장 많은 비율로 분포하고 있는 콜라겐 펩타이드는 동물의 뼈와 해양 생물의 비늘에 많이 함유되어 있다. 콜라겐은 동물 생체의 여러 결합조직에서 구조 단백질로 흔하게 발견된다. 또한, 이들은 생물 의료 자재, 제약, 화장품, 식품 및 가죽 산업에 널리 이용된다. 각종 어류 비늘에서 추출된 펩타이드는 UVB 조사에 의해 유도된 피부의 손상 및 광 노화에 대한 보호 효과가 있었다. 그럼에도 불구하고, UVB 조사에 대한 옥돔 비늘 유래의 펩타이드 특성은 명확히 알려져 있지 않다. 이 연구에서는 옥돔 비늘 추출물에서 분리된 1 kDa 이상(HMP)과 1 kDa 이하(LMP)의 펩타이드를 이용하여 UVB 조사에 의해 유도된 피부 손상과 광 노화에 대한 효과를 연구하였다. 이들 펩타이드는 농도 의존적으로 DPPH 라디칼 소거능을 보였으며 LMP는 HaCaT 인간 피부세포에서 UVB 조사에 의해 유도된 세포 지질 과산화 산물인 8-isoprostane 생성을 억제하였다. 그리고 LMP와 HMP는 B16F10 마우스 흑색종 세포에서 tyrosinase 활성 및 melanin 함량을 감소시켰으며 또한 HaCaT 세포에서 UVB로 유도된 elastase 활성을 감소시켰고 matrix metalloproteinase-1의 활성을 감소시켰다. 이러한 결과는 옥돔 비늘에서 유래된 펩타이드가 미백효과, 항산화제 및 광 노화 억제제로서 유용한 물질이 될 것으로 기대된다.

• Journal of Ginseng Research
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• 제37권4호
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• pp.389-400
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• 2013

Korean Red Ginseng (KRG) has been reported to exert anticancer, anti-oxidant, and anti-inflammatory effects. However, there has been no report on the effect of KRG on skin pigmentation. In this study, we investigated the inhibitory effect of KRG on melanocyte proliferation. KRG extract (KRGE) at different concentrations had no effect on melanin synthesis in melan-A melanocytes. Saponin of KRG (SKRG) inhibited melanin content to 80% of the control at 100 ppm. Keratinocyte-derived factors induced by UV-irradiation were reported to stimulate melanogenesis, differentiation, proliferation, and dendrite formation. In this study, treatment of melan-A melanocytes with conditioned media from UV-irradiated SP-1 keratinocytes increased melanocyte proliferation. When UV-irradiated SP-1 keratinocytes were treated with KRGE or SKRG, the increase of melanocyte proliferation by the conditioned media was blocked. Granulocyte-macrophage colony-stimulating factor (GM-CSF) was produced and released from UV-irradiated keratinocytes. This factor has been reported to be involved in regulating the proliferation and differentiation of epidermal melanocytes. In this study, GM-CSF was significantly increased in SP-1 keratinocytes by UVB irradiation ($30mJ/cm^2$), and the proliferation of melan-A melanocytes increased significantly by GM-CSF treatment. In addition, the proliferative effect of keratinocyte-conditioned media on melan-A melanocytes was blocked by anti-GM-CSF treatment. KRGE or SKRG treatment decreased the expression of GM-CSF in SP-1 keratinocytes induced by UVB irradiation. These results demonstrate that UV irradiation induced GM-CSF expression in keratinocytes and KRGE or SKRG inhibited its expression. Therefore, KRG could be a good candidate for regulating UV-induced melanocyte proliferation.

• 한국미생물·생명공학회지
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• 제47권2호
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• pp.195-200
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• 2019

The purpose of this study was to investigate the melanogenesis inhibiting activity of the ethanol extract from Polygonum amphibium L. Firstly, the n-hexane (Hx), chloroform ($CHCl_3$), ethyl acetate (EA), n-butanol (BuOH), and water (Water) fractions were isolated from the P. amphibium L. ethanol extract. The efficacy of melanogenesis was found to significantly decrease via the EA and BuOH fractions when compared to the control in B16F10 cells. EA particularly showed the lowest melanin content in B16F10 cells when compared to all the other extracts. Concentration-dependent inhibition of melanin synthesis was also observed in the EA fraction at concentrations below $50{\mu}g/ml$, which did not exhibit cytotoxicity in B16F10 cells. Notably, the expression of three key proteins (tyrosinase, tyrosinase-related protein-1 (TRP-1), and TRP-2), which are involved in melanogenesis, were significantly decreased via the EA fraction. EA also inhibited body pigmentation in vivo in a zebrafish model. Overall, we demonstrated melanogenesis suppression using the EA fraction from P. amphibium L., which could be a potential candidate for an antimelanogenesis agent.