Browse > Article
http://dx.doi.org/10.5352/JLS.2015.25.3.269

Peptides-derived from Scales of Branchiostegus japonicus Inhibit Ultraviolet B-induced Oxidative Damage and Photo-aging in Skin Cells  

Oh, Min Chang (School of Medicine, Jeju National University)
Kim, Ki Cheon (School of Medicine, Jeju National University)
Ko, Chang-ik (Research Institute of Processing of Jeju Fisher Food, Choung Ryong Fisheries Co., LTD)
Ahn, Yong Seok (Research Institute of Processing of Jeju Fisher Food, Choung Ryong Fisheries Co., LTD)
Hyun, Jin Won (School of Medicine, Jeju National University)
Publication Information
Journal of Life Science / v.25, no.3, 2015 , pp. 269-275 More about this Journal
Abstract
Collagen peptides, which are found at high concentrations in the human body, are present in animal bones and the skin of marine organisms, namely, fish scales. Collagen is the most abundant structural protein of various connective tissues in animals. Furthermore, it is widely used in biomedical material, pharmaceutical, cosmetic, food, and leather industries. Peptides extracted from scales of various fish protect against ultraviolet B (UVB)-induced skin damage and photo-aging. However, the protective effects of collagen peptides derived from the scales of Branchiostegus japonicus against UVB exposure are unclear. This study investigated the effects of peptides larger than 1 kDa (high-molecular weight peptides [HMP]) and smaller than 1 kDa (low-molecular weight peptides [LMP]), derived from extracts of B. japonicus scales, against UVB-induced skin damage and photo-aging. These peptides scavenged 1,1-diphenyl-2-picrylhydrazyl radicals in a dose-dependent manner. In UVB-exposed HaCaT human keratinocytes, LMP inhibited 8-isoprostane generation, a marker of cellular lipid peroxidation. The peptides also suppressed the UVB-induced increase in tyrosinase activity and melanin content in B16F10 mouse melanoma cells. In addition, the LMP and HMP treatment suppressed UVB-induced elastase and matrix metalloproteinase-1 activities in the HaCaT cells. These results indicate that peptides derived from B. japonicus scales have antioxidant, antiphoto-aging, and skin-whitening effects.
Keywords
B16F10 cells; Branchiostegus japonicas scales; collagen peptides; HaCaT cells; ultraviolet B;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Zlatev, Z. S., Lidon, F. J. C. and Kaimakanova, M. 2012. Plant physiological responses to UV-B radiation. Emir. J. Food Agric. 24, 481-501.   DOI
2 Sugimoto, K., Nishimura, T., Nomura, K., Sugimoto, K. and Kuriki, T. 2003. Syntheses of arbutin-alpha-glycosides and a comparison of their inhibitory effects with those of alpha-arbutin and arbutin on human tyrosinase. Chem. Pharm. Bull. (Tokyo) 51, 798-801.   DOI
3 Sugimoto, K., Nishimura, T., Nomura, K., Sugimoto, K. and Kuriki, T. 2004. Inhibitory effects of alpha-arbutin on melanin synthesis in cultured human melanoma cells and a three-dimensional human skin model. Biol. Pharm. Bull. 27, 510-514.   DOI
4 Sankar, S., Sekar, S., Mohan, R., Rani, S., Sundaraseelan, J. and Sastry, T. P. 2004. Preparation and partial characterization of collagen sheet from fish (Lates calcarifer) scales. Int. J. Biol. Macromol. 42, 6-9.
5 Tada, M., Kohno, M. and Niwano, Y. 2014. Alleviation effect of arbutin on oxidative stress generated through tyrosinase reaction with L-tyrosine and L-DOPA. BMC Biochem. 9, 15-23.
6 Tanaka, M., Koyama, Y. and Nomura, Y. 2009. Effects of collagen peptide ingestion on UVB-induced skin damage. Biosci. Biotechnol. Biochem. 73, 930-932.   DOI
7 Tsuji, N., Moriwaki, S., Suzuki, Y., Takema, Y. and Imokawa, G. 2001. The role of elastases secreted by fibroblasts in wrinkle formation: implication through selective inhibition of elastase activity. Photochem. Photobiol. 74, 283-290.   DOI
8 Matsumura, Y. and Ananthaswamy, H. N. 2002. Short-term and long-term cellular and molecular events following UV irradiation of skin: implications for molecular medicine. Expert. Rev. Mol. Med. 4, 1-22.
9 Varani, J., Dame, M. K., Rittie, L., Fligiel, S. E., Kang, S., Fisher, G. J. and Voorhees, J. J. 2006. Decreased collagen production in chronologically aged skin: roles of age-dependent alteration in fibroblast function and defective mechanical stimulation. Am. J. Pathol. 168, 1861-1868.   DOI
10 Wolber, R., Schlenz, K., Wakamatsu, K., Smuda, C., Nakanishi, Y., Hearing, V. J. and Ito, S. 2008. Pigmentation effects of solar-simulated radiation as compared with UVA and UVB radiation. Pigment. Cell Melanoma Res. 21, 487-491.   DOI
11 Moon, H. J., Lee, S. R., Shim, S. N., Jeong, S. H., Stonik, V. A., Rasskazov, V. A., Zvyagintseva, T. and Lee, Y. H. 2008. Fucoidan inhibits UVB-induced MMP-1 expression in human skin fibroblasts. Biol. Pharm. Bull. 31, 284-289.   DOI
12 Masahiro, O., Ralph, J. P., Michael, W. M., Jon B., Mark, A. S. and Jack, N. L. 2004. Biochemical properties of bone and scale collagens isolated from the subtropical fish black drum (Pogonia cromis) and sheepshead seabream (Archosargus probatocephalus). Food Chem. 88, 495-501.   DOI
13 Palmer, D. M. and Kitchin, J. S. 2010. Oxidative damage, skin aging, antioxidants and a novel antioxidant rating system. J. Drugs Dermatol. 9, 11-15.
14 Nazeer, R. A., Kumar, N. S. and Jai Ganesh, R. 2012. In vitro and in vivo studies on the antioxidant activity of fish peptide isolated from the croaker (Otolithes ruber) muscle protein hydrolysate. Peptides 35, 261-268.   DOI   ScienceOn
15 Puglia, C., Offerta, A., Saija, A., Trombetta, D. and Venera, C. 2014. Protective effect of red orange extract supplementation against UV-induced skin damages: photoaging and solar lentigines. J. Cosmet. Dermatol. 13, 151-157.   DOI
16 Chung, L., Dinakarpandian, D., Yoshida, N., Lauer-Fields, J. L., Fields, G. B., Visse, R. and Nagase, H. 2004. Collagenase unwinds triple-helical collagen prior to peptide bond hydrolysis. EMBO J. 23, 3020-3030.   DOI
17 Pati, F., Adhikari, B. and Dhara, S. 2009. Isolation and characterization of fish scale collagen of higher thermal stability. Bioresour. Technol. 101, 3737-3742.
18 Qinghui, L., Caili, W. and Congli, L. 1999. Study on the senility-delaying effect of fish scale extract on rats. Marine Fisheries Res. 20, 75-79.
19 Gragnani, A., Cornick, S. M., Chominski, V., de Noronha, S. M. R., de Noronha, S. A. A. C. and Ferreira, L. M. 2014. Review of major theories of skin aging. Adv. Aging Res. 3, 49375.
20 Hearing, V. J. and Ekel, T. M. 1976. Mammalian tyrosinase. A comparison of tyrosine hydroxylation and melanin formation. Biochem. J. 157, 549-557.   DOI
21 Hearing, V. J. and Tsukamoto, K. 1991. Enzymatic control of pigmentation in mammals. FASEB J. 5, 2902-2909.   DOI
22 Barker, D., Dixon, K., Medrano, E. E., Smalara, D., Im, S., Mitchell, D., Babcock, G. and Abdel-Malek, Z. A. 1995. Comparison of the responses of human melanocytes with different melanin contents to ultraviolet B irradiation. Cancer Res. 55, 4041-4046.
23 Hosoi, J., Abe, E., Suda, T. and Kuroki, T. 1985. Regulation of melanin synthesis of B16 mouse melanoma cells by 1 alpha, 25-dihydroxyvitamin D3 and retinoic acid. Cancer Res. 45, 1474-1478.
24 Imokawa, G. 2008. Recent advances in characterizing biological mechanisms underlying UV-induced wrinkles: a pivotal role of fibrobrast-derived elastase. Arch. Dermatol. Res. 1, 7-20.
25 Kang, S., Fisher, G. J. and Voorhees, J. J. 1997. Photoaging and topical tretinoin: therapy, pathogenesis, and prevention. Arch. Dermatol. 133, 1280-1284.   DOI
26 Kim, Y. J., No, J. K., Lee, J. H. and Chung, H. Y. 2005. 4,4'-Dihydroxybiphenyl as a new potent tyrosinase inhibitor. Biol. Pharm. Bull. 28, 323-327.   DOI
27 Labat-Robert, J., Fourtanier, A., Boyer-Lafargue, B. and Robert, L. 2000. Age dependent increase of elastase type protease activity in mouse skin: effect of UV-irradiation. J. Photochem. Photobiol. B 57, 113-118.   DOI
28 Akiu, S., Suzuki, Y., Asahara, T., Fujinuma, Y. and Fukuda, M. 1991. Inhibitory effect of arbutin on melanogenesis-biochemical study using cultured B16 melanoma cells. Nihon Hifuka Gakkai Zasshi 101, 609-613.
29 Boisnic, S., Branchet-Gumila, M. C., Nocera, T. and Verriere, F. 2005. RALGA (Diacnèal®) decreases melanin content in a human skin model. Dermatology 1, 35-38.
30 Boissy, R. E., SaKai, C., Zhao, H., Kobayashi, T. and Hearing, V. J. 1998. Human tyrosinase related protein-1 (TRP-1) does not function as DHICA oxidase activity in contrast to murine TRP-1. Exp. Dermatol. 7, 198-204.   DOI
31 Carmichael, J., DeGraff, W. G., Gazdar, A. F., Minna, J. D. and Mitchell, J. B. 1987. Evaluation of a tetrazolium-based semiautomated colorimetric assay: assessment of chemosensitivity testing. Cancer Res. 47, 936-942.