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http://dx.doi.org/10.4014/mbl.1810.10014

Whitening Activities of Ethanol Extract from Polygonum amphibium L.  

Hwang, Buyng Su (Freshwater Bioresources Utilization Bureau, Nakdonggang National Institute of Biological Resources)
Lee, Seung Young (Freshwater Bioresources Utilization Bureau, Nakdonggang National Institute of Biological Resources)
Kang, Chang Hee (Freshwater Bioresources Utilization Bureau, Nakdonggang National Institute of Biological Resources)
Han, Woog (Freshwater Bioresources Utilization Bureau, Nakdonggang National Institute of Biological Resources)
Oh, Young Taek (Freshwater Bioresources Utilization Bureau, Nakdonggang National Institute of Biological Resources)
Yu, Sang Mi (Freshwater Bioresources Utilization Bureau, Nakdonggang National Institute of Biological Resources)
Kim, Min Jin (Freshwater Bioresources Utilization Bureau, Nakdonggang National Institute of Biological Resources)
Kim, Chul Hwan (Freshwater Bioresources Utilization Bureau, Nakdonggang National Institute of Biological Resources)
Eom, Jung Hye (Freshwater Bioresources Utilization Bureau, Nakdonggang National Institute of Biological Resources)
Jeong, Sang Chul (Freshwater Bioresources Utilization Bureau, Nakdonggang National Institute of Biological Resources)
Lee, Wook Jae (Freshwater Bioresources Utilization Bureau, Nakdonggang National Institute of Biological Resources)
Ahn, Young Hee (Freshwater Bioresources Utilization Bureau, Nakdonggang National Institute of Biological Resources)
Jeong, Yong Tae (Freshwater Bioresources Utilization Bureau, Nakdonggang National Institute of Biological Resources)
Publication Information
Microbiology and Biotechnology Letters / v.47, no.2, 2019 , pp. 195-200 More about this Journal
Abstract
The purpose of this study was to investigate the melanogenesis inhibiting activity of the ethanol extract from Polygonum amphibium L. Firstly, the n-hexane (Hx), chloroform ($CHCl_3$), ethyl acetate (EA), n-butanol (BuOH), and water (Water) fractions were isolated from the P. amphibium L. ethanol extract. The efficacy of melanogenesis was found to significantly decrease via the EA and BuOH fractions when compared to the control in B16F10 cells. EA particularly showed the lowest melanin content in B16F10 cells when compared to all the other extracts. Concentration-dependent inhibition of melanin synthesis was also observed in the EA fraction at concentrations below $50{\mu}g/ml$, which did not exhibit cytotoxicity in B16F10 cells. Notably, the expression of three key proteins (tyrosinase, tyrosinase-related protein-1 (TRP-1), and TRP-2), which are involved in melanogenesis, were significantly decreased via the EA fraction. EA also inhibited body pigmentation in vivo in a zebrafish model. Overall, we demonstrated melanogenesis suppression using the EA fraction from P. amphibium L., which could be a potential candidate for an antimelanogenesis agent.
Keywords
Polygonum amphibium L.; melanogenesis; B16F10; zebrafish; ethanol extract;
Citations & Related Records
Times Cited By KSCI : 3  (Citation Analysis)
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