• 한국수정란이식학회지
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• 제28권2호
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• pp.127-132
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• 2013

The objective of this study was to examine the effect of in vitro maturation (IVM) medium, cytochalasin B (CB) treatment during intracytoplasmic sperm injection (ICSI), and electric activation on in vitro development ICSI-derived embryos in pigs. Immature pig oocytes were matured in vitro in medium 199 (M199) or porcine zygote medium (PZM)-3 that were supplemented with porcine follicular fluid, cysteine, pyruvate, EGF, insulin, and hormones for the first 22 h and then further cultured in hormone-free medium for an additional 21~22 h. ICSI embryos were produced by injecting single sperm directly into the cytoplasm of IVM oocytes. The oocytes matured in PZM-3 with 61.6 mM NaCl (low-NaCl PZM-3) tended to decrease (0.05

• Journal of Plant Biotechnology
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• 제39권4호
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• pp.305-308
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• 2012

장미 기내 신초 생육을 촉진시키고자 액체배지 첨가 실험을 실시하였다. 국립원예특작과학원 육성 신품종 '엔텍컬', '샤이니오렌지', '화이트젠', '레드젠' 등 4품종의 internode를 기내 도입한 후, 2회의 계대배양에 의해 신초를 증식하였다. 10~15 mL의 액체배지 첨가에 의해 4품종 모두 신초의 신장이 촉진되었고, 증식력에 있어서는 품종간 차이를 보였다.

• 한국수정란이식학회지
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• 제12권3호
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• pp.283-291
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• 1997

In vitro development of bovine embryos is affected by many factors such as energy substrates, amino acids, and some growth factors. It has been reported that mRNA of insulin, PDGF and their receptors are detected in cow embryos, and that some chelating agents such as EDTA and transferrin have beneficial role on mouse and bovine embryos. The author hypothesized that insulin, transferrin arid PDGF added to a culture medium increase in vitro development of bovine embryos by chelating toxic substance(s) or increasing cell growth and metabolism. Immature oocytes from slaughtered ovaries of Holstein cows and heifers were matured for 24 hours in a TCM199 containing 10% fetal calf serum, FSH, LH and estradiol with granulosa cells in vitro. Matured oocytes were coincubated with sperm for 30 hours in a modified Tyrode's medium (IVF). Embryos cleaved to 2- to 4-cell at 30 hours after IVF were selected and cultured in a 30-$\mu$l drop of a synthetic oviduct fluid medium (SOFM) containing 0.8% BSA, Minimum Essential Medium essential and non-essential amino acids, and insulin, transferrin or PDGF for 9 days. Supplementation of a SOFM with insulin, and /or transferrin did not increase develop-mental rate to expanding and hatching blastocyst of 2- to 4-cell bovine embryos compared with control. The highest developmental rate to hatching blastocyst was shown when PDGF was added at the concentration of 10 ng /ml among the supplementing doses tested in the present study (p<0.05). Addition of PDGF without insulin to a SOFM could not increase embrye development, but combined addition of PDGF with insulin significantly increased (p<0.05) embryo development to hatching blastocyst (50%) compared with control (38%). In conclusion, insulin and PDGF supplemented to a SOFM may act synergistically and have beneficial effect on in vitro development of 2- to 4-cell bovine embryos matured and fertilized in vitro.

• 대한건축학회논문집:계획계
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• 제35권4호
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• pp.127-134
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• 2019

Recently, the city development policies are being switched to the policies focusing on recession worldwide. Even though South Korea is also facing the phenomena that small and medium-sized cities are downsized because of a decrease in population and an aging society problem, there is lack of solutions and even they are still planning city development policies based on prospect of high growth. This study is featuring 4 viewpoints depending on shrinking population trend in 7 small and medium sized local cities, which are chosen as downsized cities. It is dealing with population peak, current population, 2030 population, and 2040 population viewpoints. And this study uses downsizing simulation that suggests optimum area that fits population and then shows visual check of the status of the small and medium sized local cities. The area of the small and medium sized local cities are expanded more than needs when they are on population peak point. However, they need only 20% to 25% of current area in 2030 and 2040. The most important thing is realizing the seriousness and facing up to the 'shrinking' phenomena that each small and medium-sized cities suffer. Therefore, this study has significance for presenting the status of small and medium-sized cities' problems in South Korea.

• 벤처창업연구
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• 제2권1호
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• pp.133-151
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• 2007

지난 20세기 우리나라는 단시간 내에 산업화를 이룩하여 세계 12위의 주요 경제국으로 올라섰으나, 압축 성장은 사회의 모든 부문에 심각한 불균형을 초래했으며, 특히 수도권과 경부선축 중심의 발전전략은 지역경제 간의 불균형발전, 수도권 과밀화로 인한 각종 사회 경제적 문제를 노정시키고 있다. 국민의 정부 이후 행정수도 이전, 공공기관 및 연구기관의 지방이전 정책 등을 추진하고, 지역특화산업의 도출을 통한 중소기업의 육성 및 장려, 지역 균형발전을 도모하고자 하나, 중앙정부와 지방자치단체, 각종 이해집단의 이견으로 통합적이고 조화로운 지역균형발전에 대한 해법을 제시하지 못하고 있는 실정이다. 이에 본 연구는 정부의 지역 간, 산업체간 균형발전이라는 국정목표를 수행함에 있어 우리나라 지역산업 발전의 근간을 이루고 있는 중소기업의 자생적, 창의적 발전을 위한 지역균형발전 모형을 제시함으로써, 조화롭고 통합된 국가산업정책을 도모하고, 나아가 동북아 중심국가 실현의 기초를 제공하고자 한다. 그간 우리나라의 지역 중소기업정책은 수도권에 대한 입지규제와 지방에서의 하드 인프라스트럭처 구축을 중심으로 중앙정부의 하향식 정책 집행에 의해 추진되어 왔다. 정책의 유기적 연계성이 결여된 상태에서 부처별로 예산집행이 이루어짐으로써, 지역의 입지여건에 적합한 산업군집의 형성을 통한 지역경쟁력의 강화가 효율적으로 이루어지지 못한 것이 주지의 사실로, 본 연구는 지식기반경제 하에서의 지역 중소기업정책의 의미와 지역균형발전 모형의 구축 방안, 그리고 이에 근거한 지역의 중소기업발전 방안을 제시하였다.

• 한국응용과학기술학회지
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• 제32권1호
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• pp.93-99
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• 2015

본 연구는 유채 꽃가루 분말의 영양성분 분석과 파쇄한 꽃가루 분말을 이용하여 효모 배양용 액체배지와 고체배지를 제조하여 효모 배양효율을 분석하였다. 유채 꽃가루 분말(100 g)은 탄수화물 58.9 g, 단백질 20.8 g, 지방 4.1 g, 회분 2.5 g 및 수분 13.7 g으로 구성되었다. 당질 중에서는 Fructose(13.7 g), Glucose(11.1 g)와 Sucrose(6.6 g), 미네랄 중에서는 K(606.7 mg)와 P(603.3 mg) 함향이 높았다. 유리 아미노산은 Glutamic acid(2,482.4 mg), Aspartic acid(2,136.5 mg), Lysine(1,648.3 mg), 및 Leucine(1,631.1 mg) 순으로 많았다. 유채 꽃가루 분말을 단독 또는 NaCl을 첨가하여 액체배지 및 고체배지 제조하고 효모를 배양한 결과, 꽃가루 분말이 15 g/L 이상 함유된 액체배지는 효모 배양 후 건물중이 YPD 배지(대조구)에 비해 많았고, 꽃가루 분말의 농도 의존적으로 효모 건물중이 증가하였다. 또한 액체배지에 NaCl을 1~20 g/L 추가 시 배양된 효모의 건물중이 한층 더 증가하였다. 유채 꽃가루로 제조한 고체배지를 이용하여 효모를 배양(48시간)한 결과, 콜로니(colonies) YPD 배지와 유사하게 잘 자랐다.

• 유통과학연구
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• 제11권4호
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• pp.89-97
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• 2013

Purpose - The purpose of this study is to systematically review the background of the Korean distribution promotion policy and distribution adjustment policies along with related regulations and policies. Research design, data, and methodology - Domestic distribution policy and relevant laws were examined through a review of existing research literature. The results of the development process of the domestic distribution policy, promotion policies, and adjustment policies are summarized below. Results - The results are summarized as follows. First, the purpose of the development of the domestic distribution promotion policy was to strengthen the competitiveness of the small and medium business industry through structural advancement of the small and medium industry. By expanding the managerial base for the small and medium industry, a new balance could be created in the national economy. There was a requirement for an early assistance policy for small and medium businesses as a base of these businesses in the distribution industry developed from their original model of catering to a traditional market of retail shops. Since 1996, there was a need for this early assistance policy due to the expansion and rapid growth of large scale stores causing a change in the consumption pattern for distribution markets and the decline of large enterprises. Second, the government supports small and medium business distribution through distribution promotion policies by supporting an organization promoting small business and supporting innovation in the distribution system. Third, in 1961 a business mediation system was established to protect small and medium industries. The Small and Medium Business Administration advises conglomerates to postpone acquisitions, restrain expansion of the business, or to reduce business scale if small businesses undergo an adverse effect such as decreasing demand because large companies are expanding into their areas. Fourth, the Distribution Adjustment Policy managed large-scale store regulation as follows: ① limitation on construction by urban planning ordinance, ② limitation on location based on traffic impact assessments, ③ regulation based on business guidelines by chiefs of autonomous bodies, ④ regulation on mandatory holidays and limitation of business hours. This large-scale store regulation is a policy introduced by authority to increase competitiveness of small and medium business distribution by the government. Conclusions - As discussed in this study, the distribution promotion policy and distribution adjustment policy are government distribution policies focused on the protection of the small and medium distribution businesses. This study is timely, since it was planned when the strengthening of the revisions of the Distribution Industry Development Act, aimed to protect small and medium retailers and merchants, was under discussion. The significance of this study is that it offers insights for the development of new policies in the future and an opportunity to consider the background of the distribution policy by the government.

• 한국해양공학회지
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• 제11권2호
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• pp.131-137
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• 1997

The technology of small and medium sized shipbuilding companies is lower relatively to major shipbuilding companies, and should be up-graded from the viewpoint of national economic development. In spite that hul form design is one of the most important elements with respect to shipbuilding technology, small and medium sized shipbuilding companies are incapable of developing hull form. Inorder to support technically such companies hull form development was carried out for G/T 999ton class tanker and D/W 4990ton class tanker which are the typical vessels operating in coastal area. The operating efficiency for the developed hull forms are impeoved by 30% for G/T 999ton class tanker and 24% for D/W 4990 tonclass tanker respectively. The results of hull form development including ship model test and performance analysis are presented in this paper.

• 한국가축번식학회지
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• 제14권1호
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• pp.9-16
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• 1990

This study was carried out in order to investigate effects of cryoprotectant concentration and equilibration time on survival of ultrarapidly frozen 2-cell mouse embryos. Mouse 2-cell embryos, following dehydration by exposure to DMSO and sucrose, were directly immersed into liquid nitrogen and thawed in 37$^{\circ}C$ water. Viability was defined by development rate to the blastocyst stage after in vitro culture for 72 hours. The results are summarized as follows ; 1. When 0.25M of sucrose was added into the freezing medium at various concentrations of DMSO and dilution medium, higher development rate of embryo was obtained in 3.0M DMSO concentrations (82.6%). However, when sucrose concentraitons of 0.25 and 0.5 M were added to the freezing medium with 3.0 M DMSO and dilution medium, development rate of embryos were 81.7% and 24.1%, respectively. 2. In the equilibration time at room temperature, higher development rate was attained after short period of time (2.5min) in 3.0 M DMSO＋0.25 M sucrose (85.9%). 3. The development rate of embryos at in vitro 2-cell, in vitro 2-cell, solution control and untreated control was 84.6%, 90.9%, 89.9%,, and 89.7%, respectively.

• 한국주거학회논문집
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• 제22권2호
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• pp.63-71
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• 2011

The growth of housing complex in the Housing Development District in local small and medium cities can be defined to process individual lots are possessed by buildings. The study is the basic research of analysis for the internal factors that affect on the each lot, and is for hypothesis of the verification about the Housing Development District growth. So, it can be applied in the Housing Development District of the site conditions and restriction of use of buildings and infrastructure works and the constant boundary of the region. The rate of the development is the occupation of the individual lots per entire lots was limited as the factor of analysis. As above conditions haver no possibility to change in the Housing Development District, the results of this study and further are available to consider. By the results of the connected study, it can be used for Planning and management of the Housing Development District in local small and medium cities. by the adjustment of the relation between growth tendecy and city's properties or district characters.