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http://dx.doi.org/10.12750/JET.2013.28.2.127

Effect of Oocyte Maturation Medium, Cytochalasin Treatment and Electric Activation on Embryonic Development after Intracytoplasmic Sperm Injection in Pigs  

Lee, Joohyeong (College of Veterinary Medicine, Kangwon National University)
Choi, Jung Hoon (College of Veterinary Medicine, Kangwon National University)
Lee, Seung Tae (Department of Animal Biotechnology, College of Animal Life Sciences, Kangwon National University)
Hyun, Sang-Hwan (College of Veterinary Medicine, Chungbuk National University)
Lee, Eunsong (College of Veterinary Medicine, Kangwon National University)
Publication Information
Journal of Embryo Transfer / v.28, no.2, 2013 , pp. 127-132 More about this Journal
Abstract
The objective of this study was to examine the effect of in vitro maturation (IVM) medium, cytochalasin B (CB) treatment during intracytoplasmic sperm injection (ICSI), and electric activation on in vitro development ICSI-derived embryos in pigs. Immature pig oocytes were matured in vitro in medium 199 (M199) or porcine zygote medium (PZM)-3 that were supplemented with porcine follicular fluid, cysteine, pyruvate, EGF, insulin, and hormones for the first 22 h and then further cultured in hormone-free medium for an additional 21~22 h. ICSI embryos were produced by injecting single sperm directly into the cytoplasm of IVM oocytes. The oocytes matured in PZM-3 with 61.6 mM NaCl (low-NaCl PZM-3) tended to decrease (0.05
Keywords
cytochalasin B; electric activation; embryonic development; ICSI; pig;
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