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http://dx.doi.org/10.12925/jkocs.2015.32.1.93

Making yeast culture medium with rapeseed pollen granules  

Lee, Yong-Hwa (Bioenergy Crop Research Institute, National Institute of Crop Science, Rural Development Administration)
Kim, Kwang-Soo (Bioenergy Crop Research Institute, National Institute of Crop Science, Rural Development Administration)
Jang, Young-Seok (Bioenergy Crop Research Institute, National Institute of Crop Science, Rural Development Administration)
Choi, In-Hu (Bioenergy Crop Research Institute, National Institute of Crop Science, Rural Development Administration)
Lee, Kyeong-Bo (Bioenergy Crop Research Institute, National Institute of Crop Science, Rural Development Administration)
Publication Information
Journal of the Korean Applied Science and Technology / v.32, no.1, 2015 , pp. 93-99 More about this Journal
Abstract
The aim of this study was to analyze nutritional compositions of rapeseed pollen granules and to determine the possible usage of pollen granules as a yeast culture medium. Rapeseed pollen granules (per 100 g) were consisted of carbohydrate 58.9 g, protein 20.8 g, fat 4.1 g, ash 2.5 g and water 13.7 g. And fructose (13.7 g), glucose (11.1 g), and sucrose (6.6 g) of sugars and K (606.7 mg) and P (603.3 mg) of minerals were highly contained. In addition, free amino acids such as glutamic acid (2,482.4 mg), aspartic acid (2,136.5 mg), lysine (1,648.3 mg), and leucine (1,631.1 mg) were present at a higher level. When liquid medium, which was made from cracked pollen granules (5, 10, 15, 20, 25, 30, and 40 g/L), was tested for yeast culture, liquid medium containing pollen granules over 15 g/L showed higher yeast growth than YPD medium (control). Liquid medium containing both cracked pollen granules (15 g/L) and NaCl (1 ~ 20 g/L) improved yeast growth than the liquid medium without NaCl. In addition, when yeast growth was tested on solid medium made from pollen granules (15 g/L) at $30^{\circ}C$ for 2 days, yeast colonies were equally well-formed like those grown on YPD medium. Overall, rapeseed pollen granules have potential properties on yeast growth and could be used as a primary source for yeast culture.
Keywords
Rapeseed; pollen granule; yeast; culture medium;
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