Journal of Plant Biotechnology

  • 제39권4호
  • /
  • Pages.305-308
  • /
  • 2012
  • /
  • 1229-2818(pISSN)
  • /
  • 2384-1397(eISSN)
한국식물생명공학회 (The Korean Society of Plant Biotechnology)
권호 표지
DOI QR코드

DOI QR Code

액체배지 첨가에 의한 장미 기내 신초 증식 촉진

Promotion of in vitro shoot proliferation in rose by addition of liquid medium to culture

  • 이예지 (농촌진흥청 국립원예특작과학원) ;
  • 이정림 (농촌진흥청 국립원예특작과학원) ;
  • 형남인 (상명대학교 식물식품공학과) ;
  • 김성태 (농촌진흥청 국립원예특작과학원) ;
  • 이은경 (농촌진흥청 국립원예특작과학원) ;
  • 권오현 (농촌진흥청 국립원예특작과학원) ;
  • 김원희 (농촌진흥청 국립원예특작과학원) ;
  • 이수영 (농촌진흥청 국립원예특작과학원)
  • Lee, Ye Ji (National Institute of Horticultural & Herbal Science, Rural Development Administration) ;
  • Lee, Jung Lim (National Institute of Horticultural & Herbal Science, Rural Development Administration) ;
  • Hyung, Nam-In (Department of Plant Science and Technology, Sangmyung University) ;
  • Kim, Seung Tae (National Institute of Horticultural & Herbal Science, Rural Development Administration) ;
  • Lee, Eun Kyung (National Institute of Horticultural & Herbal Science, Rural Development Administration) ;
  • Kwon, O Hyeon (National Institute of Horticultural & Herbal Science, Rural Development Administration) ;
  • Kim, Won Hee (National Institute of Horticultural & Herbal Science, Rural Development Administration) ;
  • Lee, Su Young (National Institute of Horticultural & Herbal Science, Rural Development Administration)
  • 투고 : 2012.11.26
  • 심사 : 2012.12.05
  • 발행 : 2012.12.31
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

장미 기내 신초 생육을 촉진시키고자 액체배지 첨가 실험을 실시하였다. 국립원예특작과학원 육성 신품종 '엔텍컬', '샤이니오렌지', '화이트젠', '레드젠' 등 4품종의 internode를 기내 도입한 후, 2회의 계대배양에 의해 신초를 증식하였다. 10~15 mL의 액체배지 첨가에 의해 4품종 모두 신초의 신장이 촉진되었고, 증식력에 있어서는 품종간 차이를 보였다.

To promote the growth and proliferation of in vitro rose (Rosa hybrida L) shoots, a liquid medium was added to shoot culture. Shoots were obtained by culturing internodes of four cultivars, 'Antique Curl', 'Shiny Orange', 'White Zen', and 'Red Zen', and then were proliferated by the subculture two times. An addition with 10~15 mL of liquid medium enhanced the shoot elongation of all four cultivars. However, the effect of liquid medium addition to culture of in vitro shoot for proliferation was dependent on cultivars of rose.

키워드

참고문헌

  1. Han BH, Goo DH (2003) Growth stimulation of in vitro shoots by the post-supplying of liquid medium in Anthurium andreanum 'Atlanta'. J Plant Biotechnol 30:185-188 https://doi.org/10.5010/JPB.2003.30.2.185
  2. Han BH, Yae BW, Goo DH, Shin JS (2001) Promotion of in vitro growth and rooting of micropropagated shoots in Spathiphyllum floribundum by the addition of liquid medium. J Plant Biotechnol 28:185-188
  3. Han BH, Yu HJ, Yae BW, Peak KY (2004) In vitro micro-propagation of Lilium longiglorum ‘Georgia’ by shoot formation as influenced by addition of liquid medium. Sci Hort 103:39-49 https://doi.org/10.1016/j.scienta.2004.04.020
  4. Kim CK, Oh JY, Lee SO, Chung JD (2003) In vitro micropropagation of Rosa hybrida L. J Plant Biotechnol 5:115-119
  5. Lee SY, Jung JH, Kim JH, Han BH (2008) In vitro multiple shoot proliferation and plant regeneration in rose (Rosa hybrida L.). J Plant Biotechnology. 35:223-228 https://doi.org/10.5010/JPB.2008.35.3.223
  6. Lee SY, Jung JL, Kim WH, Kim ST, Lee EK (2010) Acquirement of transgenic rose plants from embryogenic calluses via Agrobacterium tumefaciens. J Plant Biotechnol 37:511-516 https://doi.org/10.5010/JPB.2010.37.4.511
  7. Maene L, Debergh P (1985) Liquid medium additions to established tissue cultures to improve elongation and rooting in vivo. Plant Cell Tiss Org Cult 5:23-33 https://doi.org/10.1007/BF00033566
  8. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassay with tobacco tissue cultures. Physiol Plant 15:473-497 https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
  9. Pati PK, Rath SP, Sharma M, Sood A, Ahuja PS (2006) In vitro propagation of rose-A review. Biotechnol Advances 24:94-114 https://doi.org/10.1016/j.biotechadv.2005.07.001