Journal of Embryo Transfer (한국수정란이식학회지)

The Korean Society of Embryo Transfer (한국수정란이식학회)
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Effect of Oocyte Maturation Medium, Cytochalasin Treatment and Electric Activation on Embryonic Development after Intracytoplasmic Sperm Injection in Pigs

  • Lee, Joohyeong (College of Veterinary Medicine, Kangwon National University) ;
  • Choi, Jung Hoon (College of Veterinary Medicine, Kangwon National University) ;
  • Lee, Seung Tae (Department of Animal Biotechnology, College of Animal Life Sciences, Kangwon National University) ;
  • Hyun, Sang-Hwan (College of Veterinary Medicine, Chungbuk National University) ;
  • Lee, Eunsong (College of Veterinary Medicine, Kangwon National University)
  • Received : 2013.04.29
  • Accepted : 2013.05.17
  • Published : 2013.06.30
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Abstract

The objective of this study was to examine the effect of in vitro maturation (IVM) medium, cytochalasin B (CB) treatment during intracytoplasmic sperm injection (ICSI), and electric activation on in vitro development ICSI-derived embryos in pigs. Immature pig oocytes were matured in vitro in medium 199 (M199) or porcine zygote medium (PZM)-3 that were supplemented with porcine follicular fluid, cysteine, pyruvate, EGF, insulin, and hormones for the first 22 h and then further cultured in hormone-free medium for an additional 21~22 h. ICSI embryos were produced by injecting single sperm directly into the cytoplasm of IVM oocytes. The oocytes matured in PZM-3 with 61.6 mM NaCl (low-NaCl PZM-3) tended to decrease (0.05

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References

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