• BMB Reports
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• v.32 no.4
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• pp.379-384
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• 1999

Taxol, a natural product with significant anti-tumor activity, stabilizes microtubules and arrests cells in the G2/M phase of the cell cycle. It has been reported that taxol has additional effects on the cell such as an increase in tyrosine phosphorylation of proteins and activation of mitogen-activated protein (MAP) kinase. This phosphorylated kinase translocates into the nucleus and phosphorylates its substrate c-jun, c-fos, ATF2, and ATF3. The MAP kinase family is comprised of key regulatory proteins that control the cellular response to both proliferation and stress signals. First examination was cytotoxicity and apoptosis-induced concentration with paclitaxel in HeLa cell. A half-maximal inhibition of cell proliferation ($IC_{50}$) occurred at 13 nM paclitaxel. When DNA fragmentation was analyzed by agarose gel electrophoresis, a nucleosomal ladder became evident 24 h after a taxol (50 nM) addition to the cells. In addition, an apoptotic body was detected by electron microscopy. Taxol-treated cells were arrested at the S phase at 10 nM. Treatment of 50 nM taxol activated the extracellular signal-regulated protein kinase (ERK1), and a fraction of the activated MAP kinases entered the nucleus. It was also discovered that nucleus substrates c-jun was phosphorylated and activated in the cell. The activated ERK1 could subsequently translocate into the nucleus and phosphorylate its substrate c-jun as well. This study suggests that taxol-induced apoptosis might be related with signal transduction via MAP kinases.

• Proceedings of the Korean Society of Computer Information Conference
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• 2009.01a
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• pp.107-111
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• 2009

의료기관의 응급실은 환자의 생명을 다루는 긴박한 현장으로 환자에 대한 실시간 모니터링 및 관리가 필수적으로 요구되는 곳이다. 본 연구에서는 기존 응급실 진료 프로세스를 체계적으로 관리하고 모니터링 하기 위해 BPM 아키텍처를 도입하여 응급실 업무들을 표준화, 가시화함으로써 의료진의 신속한 응급 업무 대응이 가능한 응급신속대응관리시스템(EQRMS-Emergency Quick Response Management System)를 제안한다. 의료기관에서 BPM의 도입은 단순히 병원 경영 목표나 병원 내외부의 운영을 모니터링 할 수 있는 이점 이외에 병원 업무의 이윤을 극대화 할 수 있는 다양한 효과가 있다. 또한 임상위험수치 (CV-Critical Value)를 정의함으로써 복잡한 검사의 단순화와 검사 시간 단축, 검사의 오류 발생률 감소 등 환자의 안정성 제고 측면에도 크게 기여할 것이다.

• BMB Reports
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• v.47 no.4
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• pp.203-208
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• 2014

To gain insights into the effect of MexB gene under the short interfering RNA (siRNA), we synthesized 21 bp siRNA duplexes against the MexB gene. RT-PCR was performed to determine whether the siRNA inhibited the expression of MexB mRNA. Changes in antibiotic susceptibility in response to siRNA were measured by the E-test method. The efficacy of siRNAs was determined in a murine model of chronic P. aeruginosa lung infection. MexB-siRNAs inhibited both mRNA expression and the activity of P. aeruginosa in vitro. In vivo, siRNA was effective in reducing the bacterial load in the model of chronic lung infection and the P. aeruginosa-induced pathological changes. MexB-siRNA treatment enhanced the production of inflammatory cytokines in the early infection stage (P < 0.05). Our results suggest that targeting of MexB with siRNA appears to be a novel strategy for treating P. aeruginosa infections.

• Journal of Korean Neurosurgical Society
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• v.52 no.5
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• pp.435-440
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• 2012

Objective : The purpose of this study is to find the optimal stiffness and volume of bone cement and their biomechanical effects on the adjacent vertebrae to determine a better strategy for conducting vertebroplasty. Methods : A three-dimensional finite-element model of a functional spinal unit was developed using computed tomography scans of a normal motion segment, comprising the T11, T12 and L1 vertebrae. Volumes of bone cement, with appropriate mechanical properties, were inserted into the trabecular core of the T12 vertebra. Parametric studies were done by varying the volume and stiffness of the bone cement. Results : When the bone cement filling volume reached 30% of the volume of a vertebral body, the level of stiffness was restored to that of normal bone, and when higher bone cement exceeded 30% of the volume, the result was stiffness in excess of that of normal bone. When the bone cement volume was varied, local stress in the bony structures (cortical shell, trabecular bone and endplate) of each vertebra monotonically increased. Low-modulus bone cement has the effect of reducing strain in the augmented body, but only in cases of relatively high volumes of bone cement (>50%). Furthermore, varying the stiffness of bone cement has a negligible effect on the stress distribution of vertebral bodies. Conclusion : The volume of cement was considered to be the most important determinant in endplate fracture. Changing the stiffness of bone cement has a negligible effect on the stress distribution of vertebral bodies.

• Bulletin of the Korean Chemical Society
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• v.31 no.11
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• pp.3309-3312
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• 2010

D-[$^{18}F$]FMAU and L-[$^{18}F$]FMAU are F-18 labeled nucleoside analogue which have been efficiently synthesized in order to be a PET imaging probe. D-[$^{18}F$]FMAU and L-[$^{18}F$]FMAU were compared as PET imaging agents using HSV1-TK gene expressing tumor-bearing mice. Their cellular uptake profiles were also compared using MCA and MCA-TK cell lines. D-[$^{18}F$]FMAU demonstrated higher cellular uptake and higher accumulation in MCA-TK tumor regions than L-[$^{18}F$]FMAU. On the other hand, L-[$^{18}F$]FMAU showed higher MCA-TK/MCA ratio of %ID/g than that of D-[$^{18}F$]FMAU. L-[$^{18}F$]FMAU can be utilized as a good candidate for HSV1-TK PET imaging. It can be used for antiviral drug evaluation.

• KSII Transactions on Internet and Information Systems (TIIS)
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• v.7 no.5
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• pp.967-979
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• 2013

Now a day, Wireless Sensor Networks (WSNs) are being widely used in different areas one of which is healthcare services. A wireless medical sensor network senses patient's vital physiological signs through medical sensor-nodes deployed on patient's body area; and transmits these signals to devices of registered medical professionals. These sensor-nodes have low computational power and limited storage capacity. Moreover, the wireless nature of technology attracts malicious minds. Thus, proper user authentication is a prime concern before granting access to patient's sensitive and private data. Recently, P. Kumar et al. claimed to propose a strong authentication protocol for healthcare using Wireless Medical Sensor Networks (WMSN). However, we find that P. Kumar et al.'s scheme is flawed with a number of security pitfalls. Information stored inside smart card, if extracted, is enough to deceive a valid user. Adversary can not only access patient's physiological data on behalf of a valid user without knowing actual password, can also send fake/irrelevant information about patient by playing role of medical sensor-node. Besides, adversary can guess a user's password and is able to compute the session key shared between user and medical sensor-nodes. Thus, the scheme looses message confidentiality. Additionally, the scheme fails to resist insider attack and lacks user anonymity.

• Journal of Radiopharmaceuticals and Molecular Probes
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• v.5 no.2
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• pp.120-128
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• 2019

Radiopharmaceuticals include therapeutic radiopharmaceuticals and diagnostic radiopharmaceuticals. Therapeutic radiopharmaceuticals are administered to the body and ingested at specific organs to detect radiation emitted from the site and to construct an image to diagnose the disease. Diagnostic radiopharmaceuticals are used to treat diseases by killing cells with radiation emitted from radiopharmaceuticals, such as cancer cells, vascular endothelial cells, arthritis, and Alzheimer's disease. The application possibilities of terahertz imaging technology for the combination of radiopharmaceuticals and molecular imaging medicine are discussed and experimental methods are presented. Terahertz imaging is expected to be a powerful technique because of the effective piercing feasibility, which enables to perform safe and high resolutive imaging. To investigate the response of cell to the terahertz wave, both the pulsed and CW THz wave systems are employed. THz imaging of a rat's paraffin-embedded epithelial cell with tumor is studied in advance.

• The Korean Journal of Emergency Medical Services
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• v.16 no.2
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• pp.43-51
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• 2012

Purpose : We try to disclose how much the keywords of the papers from the Korean Journal of Emergency Medical Services with Medical Subject Headings(MeSH) terminologies and to understand the major subjects of the recent emergency medical technology research in Korea from keywords. Methods : We analyzed keywords from 524 articles of the Korean Journal of Emergency Medical Services that were published between 2005 and 2011. We investigated frequently used keywords and what percentages of keywords agree with MeSH terms using the MeSH browser. Results : There were on average 3.2 keywords per article. The most frequent key words were AED, Attitude, Cardiopulmonary Resuscitation, CPR, EMT, EMT students, External Defibrillator, Job satisfaction, Knowledge, 119 EMT in order. The number of terms in precise agreement with MeSH headings was 101(19.3%); 327 terms(62.4%) were not found in the MeSH browser and 96 terms(18.3%) partially matched MeSH terms. Conclusion : Many keywords used in the Korean Journal of Emergency Medical Services did not agree with MeSH terms. We conclude that contribution rules should be using MeSH terms and authors should be educated in the proper use of MeSH terms in their research and subsequent publication.

• KSII Transactions on Internet and Information Systems (TIIS)
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• v.12 no.12
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• pp.6177-6200
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• 2018

In order to achieve the goal of protecting medical images, some existing watermark techniques for medical image protection mainly focus on improving the invisibility and robustness properties of the method, in order to prevent unnecessary medical disputes. This paper proposes a novel copyright method for medical image protection based on visual secret sharing (VSS) and cellular automata transform (CAT). This method uses the protected medical image feature as well as VSS and a watermark to produce the ownership share image (OSI). The OSI is used for medical image verification and must be registered to a certified authority. In the watermark extraction process, the suspected medical image is used to generate a master share image (MSI). The watermark can be extracted by combining the MSI and the OSI. Different from other traditional methods, the proposed method does not need to modify the medical image in order to protect the copyright of the image. Moreover, the registered OSI used to verify the ownership and its appearance display meaningful information, facilitating image management. Finally, the results of the final experiment can prove the effectiveness of our method.

• Nuclear Engineering and Technology
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• v.56 no.2
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• pp.753-761
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• 2024

Introduction: In this study, TLD 600 and TLD 700 pairs were used to measure the neutron dose of Elekta Precise medical linac. To this end, the optimum moderate thickness for the conversion of fast to thermal neutrons were evaluated. Materials and methods: ²⁴¹Am-Be and ²⁵²Cf sources were simulated to calculate the optimum thicknesses of the moderator for the conversion of maximum fast neutrons (FN) into thermal neutrons (TN). Pair TLDs were used to measure F&TN doses for three different field sizes at four depths of the medical linac. Results: The maximum thickness of the moderator was optimized at 6 cm. The measurement results demonstrated that the TN dose increased with the expansion of field size and depth. The FN dose, which was converted TN, exhibits behaviors comparable to the TN due to its nature. Conclusion: This study presents the optimum thickness for the moderator to convert FN into TN and measure F&TN using TLDs.