• Journal of Life Science
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• v.25 no.2
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• pp.189-196
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• 2015

Bacterial diversity was studied in the rhizosphere of Suaeda japonica Makino, which is native to Suncheon Bay in South Korea. Soil samples from several sites were diluted serially, and pure isolation was performed by subculture using marine agar and tryptic soy agar media. Genomic DNA was extracted from 29 pure, isolated bacterial strains, after which their 16S rDNA sequences were amplified and analyzed. Phylogenetic analysis was performed to confirm their genetic relationship. The 29 bacterial strains were classified into five groups: phylum Firmicutes (44.8%), Gamma proteobacteria group (27.6%), Alpha proteobacteria group (10.3%), phylum Bacteriodetes (10.3%), and phylum Actinobacteria (6.8%). The most widely distributed genera were Bacillus (phylum Firmicutes), and Marinobacterium, Halomonas, and Vibrio (Gamma proteobacteria group). To confirm the bacterial diversity in rhizospheres of S. japonica, the diversity index was used at the genus level. The results show that bacterial diversity differed at each of the sampling sites. These 29 bacterial strains are thought to play a major role in material cycling at Suncheon Bay, in overcoming the sea/mud flat-specific environmental stress. Furthermore, some strains are assumed to be involved in a positive interaction with the halophyte S. japonica, as rhizospheric flora, with induction of growth promotion and plant defense mechanism.

• Molecules and Cells
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• v.38 no.11
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• pp.950-958
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• 2015

BCS1L gene encodes mitochondrial protein and is a member of conserved AAA protein family. This gene is involved in the incorporation of Rieske FeS and Qcr10p into complex III of respiratory chain. In our previous study, AluYRa2-derived alternative transcript in rhesus monkey genome was identified. However, this transcript has not been reported in human genome. In present study, we conducted evolutionary analysis of AluYRa2-exonized transcript with various primate genomic DNAs and cDNAs from humans, rhesus monkeys, and crabeating monkeys. Remarkably, our results show that AluYRa2 element has only been integrated into genomes of Macaca species. This Macaca lineage-specific integration of AluYRa2 element led to exonization event in the first intron region of BCS1L gene by producing a conserved 3' splice site. Intriguingly, in rhesus and crabeating monkeys, more diverse transcript variants by alternative splicing (AS) events, including exon skipping and different 5' splice sites from humans, were identified. Alignment of amino acid sequences revealed that AluYRa2-exonized transcript has short N-terminal peptides. Therefore, AS events play a major role in the generation of various transcripts and proteins during primate evolution. In particular, lineage-specific integration of Alu elements and species-specific Alu-derived exonization events could be important sources of gene diversification in primates.

• The Journal of the Korea Contents Association
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• v.13 no.4
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• pp.459-468
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• 2013

With advancements in augmented reality technology, attempts to apply it in various fields have increased. With advancements in information technology and a growth in the number of Korean learners in Korea and abroad, there is a growing demand for state-of-the-art Korean learning contents. Because the Korean learning contents developed using augmented reality have been found insufficient, this paper investigates ways to develop better contents. This paper proposes ways to develop learning contents that can increase learners' interest in learning and lead to academic achievement, because the methods of education in Korea are limited to textbook learning and learning through the media. Because of the structure of fairytales and the educational lessons that can be learned from them, Korean learning contents are developed using fairytales. Additionally, Korean contents are designed to implement augmented reality technology, and learners need only have computers, webcams, and markers to make use of it. In consideration of Korean learners who do not have access to augmented reality technology, this paper clarifies that there should be a harmony between existing and new Korean learning contents. On the basis of this study, further studies on Korean education exploring the role of augmented reality should be conducted so that Korean learning contents that use diverse types of augmented reality technology can be will developed.

• Journal of Digital Convergence
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• v.12 no.5
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• pp.403-413
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• 2014

This study is to select educational films suitable for enhancing creativity and personality in order to develop educational contents aimed at pre-service early childhood teacher. First, three film critics based on sub-elements of ego-identity selected final 59 films. Next, three education experts classified the 59 films into small groups based on components each for creativity and personality. As a result, first, films classified according to pre-service early childhood teacher' sub-elements of ego-identity, educational components for creativity, and educational components for personality were 8 in job, 7 in religion, 6 in politics, 7 in philosophical life style, 8 in friendship, 8 in relationship, 7 in gender role, and 8 in leisure activity. Second, verifying suitability of film critics and education experts using Delphi method in order to select educational films to enhance pre-service early childhood teacher' creativity and personality based on films was effective in increasing validity of the results of this research.

• The Korean Journal of Ecology
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• v.22 no.1
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• pp.39-43
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• 1999

Serratia marcescens, an enterobacterium of gram-negative bacteria, is characterized by resistance of the admium. Cadmium sensitive PM strain did not grow in the medium at cadmium concentration of 50 ppm. PA strain was induced to accommodate to cadmium by cultivating the mother strain (PC strain) in the medium with 50 ppm cadmium. As compared with PC and PM strains, PA strain revealed the excellent growth in cadmium media and accumulated four to five times higher cadmium concentration in cell than other strains. PA strain accumulated 23% of cadmium in cells when cultured in medium treated with 100 ppm cadmium and this cadmium was more accumulated in cytosol fractions than membrane fractions. Analysis by TEM indicated that cadmium was concentrated as a form of granule in cytosol. In protein patterns of cell after the treatment of cadmium, two inducible proteins (28 KDa and 64 KDa) and one reducible protein (45 KDa) were detected by SDS-PAGE. By Atomic Absorption Spectrophotometer, the amounts of cadmium attached to inducible proteins of 28 KDa and 64 KDa were 318.28 ㎍ and 325.37 ㎍ per gram of protein, respectively. It is assumed that these inducible proteins play an important role in the mechanism of cadmium accumulation in cells. A plasmid of 23Kbp was found in S. marcescens. The ability of resistance to cadmium in plasmid was confirmed by curing experiments.

• Journal of the Korean earth science society
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• v.26 no.8
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• pp.777-789
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• 2005

Study courses using cognitive conflict strategy about geological stratum were performed on the 6th grade elementary students and their conceptual changes were investigated. Some misconceptions about geological stratum that the students have are as follows: (1) stratum was formed due to volcanic eruption, (2) gneiss composes the geological stratum, (3) folds in the stratum were formed due to vertical pressure from the top. The classes had positive effects on the changes of some the misconceptions: (1) the places where the stratum is filmed, (2) the kind of rock in the stratum, and (3) the reason for folding. However, there were no significant changes in the following items: (1) the reason that every layer consists of different sizes of grains. It shows that some misconceptions can be changed easily by cognitive conflict strategy, while others can not. We checked the students who didn't change their misconceptions after the test. These students have very strong misconceptions that most of the natural phenomena on the earth's surface are due to the internal heat pressure, and volcanic activity. Another affecting the students' misconceptions are the role of teachers in class and mass media, such as TV.

• Korean Chemical Engineering Research
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• v.55 no.2
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• pp.201-213
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• 2017

Water is removed from crude oil containing water by using oil separator. This study aims to develop a three-dimensional (3D) Eulerian computational fluid dynamics (CFD) model to predict the separation efficiency of air-water-oil separator. In the incompressible, isothermal and unsteady-state CFD model, air is defined as continuous phase, and water and oil are given as dispersed phase. The momentum equation includes the drag force, lift force and resistance force of porous media. The standard k-${\varepsilon}$ model is used for turbulence flow. The exit pressures of water and oil play an important role in determining the liquid level of the oil separator. The exit pressures were identified to be 6.3 kPa and 5.1 kPa for water and oil, respectively, to keep a liquid level of 25 cm at a normal operating condition. The time evolution of volume fractions of air, water and oil was investigated. The settling velocities of water and oil along the longitudinal separator distance were analyzed, when the oil separator reached a steady-state. The oil separation efficiency obtained from the CFD model was 99.85%, which agreed well with experimental data. The relatively simple CFD model can be used for the modification of oil separator structure and finding optimal operating conditions.

• Journal of the Korea Society of Computer and Information
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• v.18 no.1
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• pp.31-42
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• 2013

This research sheds light on the non-intrusive traits of mobile devices (Electronic Guidebook, Rememberer, I-Guides and eXspot) deployed in Exploratorium for enhancing visitor experience via case studies. In an interactive exhibition environment, non-intrusiveness was the key to supporting the immersive experience and meaning-making for visitors. The usability of hand-held devices directly impacted on the non-intrusiveness, thereby reshaping the form-factors of mobile devices. The change in from-factor has also minimized the functions of devices as the remember of museum experience. Furthermore, the role of mobile devices, which turned from a supposed multi-media guide to a mere rememberer, made them virtually impossible for realizing the "seamless visiting model" originally planned. An array of projects carried out in Exploration have achieved some degree of success such as increasing viewing time as well as reinforcing post-visit activities. However, taken from musicological perspective, increase in viewing time is by all means insufficient to be taken as proof since it is assumed to be achieved by photo-taking (i.e. MyExploratorium) rather than by interacting between visitors and exhibits. This issue --increased viewing time -- needs to be analyzed in depth. All in all, mobile devices used in Exploratorium can be defined as a learning tool/educational supporting medium based on personalization for (visitors') optimizing extended museum experience.

• The Korean Journal of Zoology
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• v.31 no.3
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• pp.177-184
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• 1988

The pattern of progesterone production and secretion of frog(R. dybowskii) follicles was investigated in follicle culture in vitro. Involvement of cAMP in the regulation of the steroid production by the follicles was also investigated by manipulating endogeneous cAMP level with forskolin and/or 3-isoburyl- 1 - methylxanthine(IBMX). Endogeneous follicular progesterone level increased rapidly in one hour of culture by treatment of frog pituitary homogenate(FPH) and reached peak level at 2 hours or later. But the absolute amount of progesterone produced (60-300 pg/follicle) or the peak time of the honnone level was different between individual animals. Basal level of progesterone in untreated sister follicles was very low (around 10 pg/follicle) and nearly undetectable in most cases regardless of culture lime. Secretion level of progesterone by the follicles obtained by measunng the honnone in the culture media was just the reflection of the intrafollicular level. Exogeneously added forskolin, an adenylate cyclase stimulator, and/or IBMX, a phosphodiesterase inhibitor, could mimic FPH action in terms of progesterone production and secretion. Thus, it seems clear that FPH regulates progesterone production via cAMP system in the follicle cells.

• Journal of Digital Convergence
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• v.15 no.2
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• pp.285-294
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• 2017

In this study, change of the view of love was analyzed by big data analysis in TV drama of married person's love. Two dramas were selected for analysis with opposite theme of love story. The sympathy of audience for the one month period from the end of the drama was analyzed by text mining and sentiment analysis. In particular, changes in the meaning of home meaning are identified. Home is not 'a place where a husband and wife play a social role', but 'a place where they can share real sympathy and one can be happy'. If individuals are not happy, they need to break their homes. In this study, the current divorce rate and the question regarding the matter should be considered. But based on Google Trends, in Korean society, interest in marriage were still higher than romance. It means that people prefer to 'a love to get marriage' in Korean modern society, than 'love for love affair'. It seems to be reflection of cognition change, marriage should be based on true love. This study is expected to be applied to the study of trend change through social media.