• Mass Spectrometry Letters
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• v.9 no.1
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• pp.17-23
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• 2018

Studies on the interactions of amyloidogenic proteins with trace metals, such as copper, have indicated that the metal ions perform a critical function in the early oligomerization process. Herein, we investigate the effects of Cu(II) ions on the active sequence regions of amyloidogenic proteins using electrospray ionization mass spectrometry (ESI-MS) and collision induced dissociation tandem MS (CID-MS/MS). We chose three amyloidogenic peptides NNQQNY, LYQLEN, and VQIVYK from yeast prion like protein Sup35, insulin chain A, and tau protein, respectively. [Cu-peptide] complexes for all three peptides were observed in the mass spectra. The mass spectra also show that increasing Cu(II) concentrations decrease the population of existing peptide oligomers. The tandem mass spectrum of NNQQNY shows preferential binding for the N-terminal region. All three peptides are likely to appear to be in a Cu-monomer-monomer (Cu-M-M) structure instead of a monomer-Cu-monomer (M-Cu-M) structure.

• Mass Spectrometry Letters
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• v.10 no.1
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• pp.32-37
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• 2019

The ${\pi}-{\pi}$ interactions of the peptide-dimer and peptide-trimer complexes were investigated in the (VQIVYK + LYQLEN) and (VQIVYK + NNQQNY) mixing solutions. The results showed that tyrosine (Y) residues were critical in the formation of hetero peptide-dimers and -trimers during the early oligomerization process. We used collision-induced dissociation (CID) along with electrospray ionization mass spectroscopy (ESI-MS) to obtain the structural information of the hetero-dimers and -trimers. We chose three amyloidogenic peptides-VQIVYK, NNQQNY, and LYQLEN-from tau protein, yeast prion-like protein Sup35, and insulin chain A, respectively. Hetero-dimer, -trimer, -tetramer, and -pentamer complexes were observed in the mass spectra. The tandem mass spectrum of the hetero-dimer and hetero-trimer showed two different fragmentation patterns (covalent and non-covalent bond dissociation). Y-Y interaction structures were also proposed for the hetero-dimer and -trimer complexes.

• The Bulletin of The Korean Astronomical Society
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• v.44 no.2
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• pp.60.3-60.3
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• 2019

Binary systems of a white dwarf showing mass transfer activities are classified into cataclysmic variables and symbiotic stars. In the case of cataclysmic variables, the companion is usually a late type main sequence star filling its Roche lobe, where material is transferred through the inner Lagrangian point to form an accretion disk around the white dwarf. The disk becomes unstable and highly viscous when the surface density exceeds the critical density, leading to dwarf nova outbursts. In contrast, symbiotic stars are wide binary systems having a giant as the mass donor. Some fraction of giant stellar wind is accreted to the white dwarf giving rise to various symbiotic activities. In particular, half of symbiotics show Raman O VI at 6830 and 7088, which are important spectroscopic probe of mass transfer process. Monitoring observations using 1 m class telescopes will produce valuable information regarding the mass loss and mass transfer to white dwarf stars, shedding much light on the last stage of stellar evolution of low and intermediate mass stars.

• Proceedings of the KSME Conference
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• 2003.11a
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• pp.532-537
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• 2003

Gas flow through orifice is encountered in many diverse fields of engineering applications. In order to investigate the critical gas flow through an orifice system, a computational analysis is performed using axisymmetric, compressible, Navier-Stokes equations which are numerically solved by a fully implicit finite volume method. In the present study, the discharge coefficients of two different types of orifices which are a straight-bore orifice and a sharp-edged orifice, are predicted to obtain the critical flow conditions. The present CFD data are compared with the previous experimental results. The present computational results show that the critical mass flow rate through orifice is well predicted and it is a strong function of Reynolds number. The discharge coefficient increases with the orifice diameter.

• Transactions of the Korean Society of Automotive Engineers
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• v.23 no.4
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• pp.361-370
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• 2015

There will inevitably be errors and uncertainties in tire yaw mark related critical speed formula, which is derived merely from the relationship between the centrifugal force and the friction force acting on the point-mass vehicle. Constructing and measuring yaw marks through appropriate simulation works have made it possible to perform uncertainty analysis in calculation of critical speeds under variation of variety of conditions and parameters while existing yaw mark experimental tests have not performed properly. This paper does not present only the critical speed analysis results for parametric sensitivity and uncertainty of chord and middle ordinate, coefficient of friction and road grade, but also modeling uncertainty such as variation of braking level during turning and vehicle size. The yaw mark analysis methods and results may be now applied in practice of traffic accident investigation.

• Transactions of the Korean Society for Noise and Vibration Engineering
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• v.13 no.3
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• pp.202-209
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• 2003

A simply supported beam subjected to a uniformly distributed tangential follower force and the two successively moving spring-mass systems upon it constitute this vibration system. The influences of the velocities of the moving spring-mass system, the distance between two successively moving spring-mass systems and the uniformly distributed tangential follower force have been studied on the dynamic behavior of a simply supported beam by numerical method. The uniformly distributed tangential follower force is considered within its critical value of a simply supported beam without two successively moving spring-mass systems, and three kinds of constant velocities and constant initial displacement of two successively moving spring-mass systems are also chosen. Their coupling effects on the transverse vibration of the simply supported beam are inspected too. In this study the simply supported beam is deflected with small vibration proportional to natural frequency of the moving spring-mass systems. According to the increasing of initial displacement of the moving spring-mass systems the amplitude of the small vibration of the simply supported beam is increased due to the spring force. The velocity of the moving spring-mass system more affect on the transverse deflection of simply supported beam than other factors of the system and the effect is dominant at high velocity of the moving spring-mass systems.

• Transactions of the Korean Society of Mechanical Engineers
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• v.8 no.2
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• pp.119-126
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• 1984

An analysis is presented for the vibration and stability of Beck's column carring a tip mass at its free and subjected there to a follower compressive force by using variational approach. The influence of transverse shear deformation and rotatory inertial of the mass of the column upon the critical flutter load and frequency is considered, and Timoshenko's shear coefficient K' is calculated by Cowper's formulae. It is, moreover, worth noticing that the influence of inertial moment of tip mass upon the flutter load and frequency is investigated. The centroid of a tip mass is offset from the free end of the beam and located along its extended axis of the two cases, one of which has a tip mass increasing as .xi., the tip mass offset parameter, is augmented, the other has a tip mass constant but the inertial moment is variable according to a magnitude of .eta., the tip mass offset parament. This study reveals that the effects of inertial moment of a tip mass and larger value of P are specially remarkable even a tip mass is a same.

• The Bulletin of The Korean Astronomical Society
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• v.45 no.1
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• pp.42.4-43
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• 2020

The hydrogen-rich envelope mass of a dying massive star is the key factor that determines the type and properties of the resulting supernova. Emulating wind-driven mass loss of single stars with the MESA(Modules for Experiments in Stellar Astrophysics) stellar evolution code, we made a grid of models for a large parameter space of initial mass (12 M⊙ to 30M⊙), metallicity (solar, LMC and SMC), hydrogen envelope mass (0.01M⊙ to 10M⊙) for progenitor stars in their final step of evolution. Our results suggest the final luminosity of the progenitor is largely determined by the initial mass, which means there is luminosity degeneracy for stars with the same initial mass but with different hydrogen-rich envelope masses. Since we can break this degeneracy by correcting luminosity with surface gravity (spectroscopic HR diagram), we can infer the exact mass property of an observed progenitor. The surface temperature drastically varies near the envelope mass of ~0.1M⊙ and surface temperature of ~10000 K, where the demarcation between the hydrogen-rich envelope and the helium core lies, which explains the rarity of 'white' supergiants. There also exists a discontinuity in the chemical composition of the progenitor envelope around this critical hydrogen-rich envelope mass of ~0.1 M⊙, which can be tested in future observations of "flash spectroscopy" of supernovae.

• Journal of the Korean Society for Aeronautical & Space Sciences
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• v.33 no.4
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• pp.27-34
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• 2005

The paper discussed on the stability of cantilevered pipe conveying fluid subjected to distributed follower force. Governing equations of motion are derived by extended Hamilton's principle, and the numerical scheme using finite element method is applied to obtain the discretized equations. The critical flow velocity as a function of the distributed follower force for the various mass ratio is determined. The flutter configurations of the pipes at the critical flow velocities are drawn graphically at every twelfth period to define the order of quasi-mode of flutter configuration The critical mass ratios, at which the transference of the eigenvalue branches related to flutter take place, are definitely determined. Also, the effect of damping on the stability of the system is considered.

• Korean Journal of Air-Conditioning and Refrigeration Engineering
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• v.13 no.8
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• pp.674-681
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• 2001

An experiment is conducted to investigate the effect of the inclination angle on convective boiling heat transfer of a uniformly heated tube. The test section used is a stainless steel tube with10.7mm in inner diameter. The hating length is 3m and is heated directly by an AC current. The test fluid is R-113. Experiment are carried out with mass flow rates of 300, 500 and $700\;kg/m^{2}s$, and heat fluxes varying from 5 to 65 kW/$m^2$. The inclination angles of the tube are $0^{\circ},\;5^{\circ},\;11^{\circ}\;and\;25^{\circ}$. the circumferential temperature variation at low quality region and the location of dryout at high quality region are mainly observed. Circumferential anisothermality occurring at low mass flow rate and low quality conditions is gradually reduced with the increase in the inclination angle and finally disappears at the inclination angle of $25^{\circ}$. Critical quality where dryout is initiated is seriously influenced by the inclination angle. Wall temperature after critical quality is also affected by the inclination angle.