• Applied Microscopy
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• v.30 no.4
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• pp.403-412
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• 2000

Urechis unicinctus spermatogenic cells, sperm and spermatids, prepared from testis are investigated to identify nuclear actin using amoeba monorlonal anti-actin as the first Ab and gold particles (10 nm) conjugated mouse IgG (immunogold) as the Ab marker. The Ag-Ab reactions analyzed the localization of nuclear actin of the spermatogenic cells and the immunogold particles incorporated mainly with nuclear matrices. A few immunogold particles are merged into the acrosomes and the other architectures of spermatogenic cells, such as mitochondrion and centrioles. It is often observed and there is a tendency in which the incorporated immunogold particles are increased in number in the nuclear matrices of sperm compared with that of spermatids The increments and decrements of the incorporated immunogold particles according to developmental stages and the spermatogenic architec-tures are interpreted and discussed in aspect of acrosomal function and of nuclear condensation of spermatids.

• 한국전산유체공학회:학술대회논문집
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• 2009.11a
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• pp.1-1
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• 2009

In this presentation, I talk about various fluid simulation methods that have been developed for computer graphics special effects since 1996. They are all based on CFD but sacrifice physical reality for visual plausability and time. But as the speed of computer increases rapidly and the capability of GPU (graphics processing unit) improves, methods for more physical realism have been tried. In this talk, I will focus on four aspects of fluid simulation methods for computer graphics: (1) particle level-set methods, (2) particle-based simulation, (3) methods for exact satisfaction of incompressibility constraint, and (4) GPU-based simulation. (1) Particle level-set methods evolve the surface of fluid by means of the zero-level set and a band of massless marker particles on both sides of it. The evolution of the zero-level set captures the surface in an approximate manner and the evolution of marker particles captures the fine details of the surface, and the zero-level set is modified based on the particle positions in each step of evolution. (2) Recently the particle-based Lagrangian approach to fluid simulation gains some popularity, because it automatically respects mass conservation and the difficulty of tracking the surface geometry has been somewhat addressed. (3) Until recently fluid simulation algorithm was dominated by approximate fractional step methods. They split the Navier-Stoke equation into two, so that the first one solves the equation without considering the incompressibility constraint and the second finds the pressure which satisfies the constraint. In this approach, the first step introduces error inevitably, producing numerical diffusion in solution. But recently exact fractional step methods without error have been developed by fluid mechanics scholars), and another method was introduced which satisfies the incompressibility constraint by formulating fluid in terms of vorticity field rather than velocity field (by computer graphics scholars). (4) Finally, I want to mention GPU implementation of fluid simulation, which takes advantage of the fact that discrete fluid equations can be solved in parallel.

• Journal of the Korean Society for Marine Environment & Energy
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• v.2 no.2
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• pp.70-77
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• 1999

The disturbance of oil-water interface confined between tandem fences caused by a sequence of traveling vortices below the interface is investigated. The traveling vortices are assumed to be those detached from the tip of the fore fence. The potential flow is assumed and the density interface is replaced as a sheet of vortex. The shape of the interface is predicted by tracing a finite number of marker particles placed at the interface. The velocity of the marker particles is determined by the Biot-Savart integral along the vortex sheet plus the contribution from the traveling point vortices. The rate of change of vortex-sheet strength is predicted by using an evolution equation for vorticity. The calculated results obtained for various conditions demonstrate that the large amplitude of interfacial wave following the moving vortek can be generated by the vortices.

• Journal of computational fluids engineering
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• v.4 no.1
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• pp.19-26
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• 1999

This paper describes a numerical method for predicting the incompressible unsteady laminar three-dimensional flows with free-surface. The Navier-Stokes equations governing the flows have been discretized by means of finite-difference approximations, and the resulting equations have been solved via the SIMPLE-C algorithm. The free-surface is defined by the motion of a set of marker particles and the interface behaviour was investigated by means of a "Lagrangian" technique. Using the GALA concept of Spalding, the conventional mass continuity equation is modified to form a volumetric or bulk-continuity equation. The use of this bulk-continuity relation allows the hydrodynamic variables to be computed over the entire flow domain including both liquid and gas regions. Thus, the free-surface boundary conditions are imposed implicitly and the problem formulation is greatly simplified. The numerical procedure is validated by comparing the predicted results of a periodic standing waves problems with analytic solutions. The results show that this numerical method produces accurate and physically realistic predictions of three-dimensional free-surface flows.

• Journal of the Society of Naval Architects of Korea
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• v.54 no.1
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• pp.26-33
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• 2017

In this study, the surface marker method, one of the particle tracking methods, used to track the free surface is extended to cover the more general cases easily including the collision and separation of the free surface. In surface particle method to redistribute particles effectively using the grid system, the free surface is composed of the sum of quadrilaterals having four curves where fixed markers are placed at ends of each curve. Fixed markers are used to know how curves are connected to each other. The position of fixed markers can move as the free surface deforms but all fixed markers cannot be deleted during all time of simulation to keep informations of curve connection. In the case of the collision or separtion of the free surface where several curves can be intersected disorderly, severe difficulties can occur to define newly states of curve connection. In this study, the adaptable surface parTicle method without fixed markers is introduced. Intersection markers instead of the fixed markers are used to define quadrilaterals. The position of the intersection markers is defined to be the intersection point between the free surface and the edge of the grid and it can be added or deleted during the time of simulation to allow more flexibilities. To verify numerical schemes, two flow cases are simulated and the numerical results are compared with other's one and shown to be valid.

• KIPS Transactions on Software and Data Engineering
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• v.8 no.2
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• pp.79-88
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• 2019

Machine translation (MT) system, especially Korean-Mongolian MT system, has recently attracted much attention due to its necessary for the globalization generation. Korean and Mongolian have the same sentence structure SOV and the arbitrarily changing of their words order does not change the meaning of sentences due to postpositional particles. The particles that are attached behind words to indicate their grammatical relationship to the clause or make them more specific in meaning. Hence, the particles play an important role in the translation between Korean and Mongolian. However, one Korean particle can be translated into several Mongolian particles. This is a major issue of the Korean-Mongolian MT systems. In this paper, to address this issue, we propose a method to use the combination of UTagger and a Korean-Mongolian particles table. UTagger is a system that can analyze morphologies, tag POS, and disambiguate homographs for Korean texts. The Korean-Mongolian particles table was manually constructed for matching Korean particles with those of Mongolian. The experiment on the test set extracted from the National Institute of Korean Language's Korean-Mongolian Learner's Dictionary shows that our method achieved the accuracy of 88.38% and it improved the result of using only UTagger by 41.48%.

• IMMUNE NETWORK
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• v.12 no.3
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• pp.104-112
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• 2012

Silica is one of the most abundant compounds found in nature. Immoderate exposure to crystalline silica has been linked to pulmonary disease and crystalline silica has been classified as a Group I carcinogen. Ultrafine (diameter <100 nm) silica particles may have different toxicological properties compared to larger particles. We evaluated the effect of ultrafine silica nanoparticles on mouse bone marrow-derived dendritic cells (BMDC) and murine dendritic cell line, DC2.4. The exposure of dendritic cells (DCs) to ultrafine silica nanoparticles showed a decrease in cell viability and an induction of cell death in size- and concentration-dependent manners. In addition, in order to examine the phenotypic changes of DCs following co-culture with silica nanoparticles, we added each sized-silica nanoparticle along with GM-CSF and IL-4 during and after DC differentiation. Expression of CD11c, a typical DC marker, and multiple surface molecules such as CD54, CD80, CD86, MHC class II, was changed by silica nanoparticles in a size-dependent manner. We also found that silica nanoparticles affect inflammatory response in DCs in vitro and in vivo. Finally, we found that p38 and NF-${\kappa}B$ activation may be critical for the inflammatory response by silica nanoparticles. Our data demonstrate that ultrafine silica nanoparticles have cytotoxic effects on dendritic cells and immune modulation effects in vitro and in vivo.

• Journal of Nutrition and Health
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• v.55 no.2
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• pp.240-249
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• 2022

Purpose: Glabridin (GD) is a bio-available isoflavane isolated from the root extract of licorice (Glycyrrhiza glabra L.). It exhibits a variety of pharmacological activities such as anti-inflammatory and anti-oxidant activities. However, extracellular vesicles (EVs) secretion and the anti-cancer mechanism of action remains largely unknown. The present study investigates the anticancer effects of GD by determining the inhibition of EVs secretion in the human breast cancer cell line, MDA-MB-231. Methods: Cell viability, reactive oxygen species (ROS) production, migration, invasion rate, and vascular endothelial growth factor (VEGF) concentration were assessed in MDA-MB-231 cells treated with increasing concentrations of GD (0.1, 1, 5, 10, 20 µM). Subsequently, EV secretion and exosomal DEL-1 protein expression were evaluated to determine the anticancer effects of GD. Results: The results showed that GD significantly inhibited the cell proliferation of MDA-MB-231 cells in a dose- or time-dependent manner. Also, ROS production and apoptosis marker protein cleaved caspase-3 were significantly increased in GD-treated MDA-MB-231, compared to control. Furthermore, GD exposure resulted in significantly decreased not only migration and invasion rates but also the VEGF concentration, thereby contributing to a reduction in angiogenesis. Interestingly, the concentration and number of EVs as well as EV marker proteins, such as CD63 and TSG101, were decreased in GD-treated MDA-MB-231 cells. Markedly, extracellular matrix protein DEL-1 as angiogenesis factor was decreased in EVs from GD-treated MDA-MB-231 cells. Conclusion: This study identifies that the anti-cancer molecular mechanism of GD is exerted via inhibition of angiogenesis and EVs secretion, indicating the potential of GD as a chemotherapeutic agent for breast cancer.

• Journal of computational fluids engineering
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• v.19 no.1
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• pp.57-63
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• 2014

This study performed numerical analysis for the characteristics of flow-induced forces and the flow instability depending on the cross-sectional shape of the cylinder in laminar flow. To implement the cylinder cross-section, we adopted an Immersed Boundary Method with marker particles. We analyzed flow characteristics based on the radius of corner curvature. Main parameters are corner radius and Reynolds number (Re). With Re = 40, 50, 150 we calculated the flow field, drag coefficient, RMS of lift coefficient, pressure coefficient and Strouhal number in conjunction with the corner radius variation. Also, we calculated critical Reynolds number ($Re_c$) depending on the corner radius variation.

• 한국전산유체공학회:학술대회논문집
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• 1998.05a
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• pp.156-161
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• 1998

This describes a numerical method for predicting the incompressible unsteady laminar three-dimensional flows of fluid behaviour with free-surface. The elliptic differential equations governing the flows have been linearized by means of finite-difference approximations, and the resulting equations have been solved via a fully-implicit iterative method. The free-surface is defined by the motion of a set of marker particles and interface behaviour was investigated by way of a 'Lagrangian' technique. Using the GALA concept of Spalding, the conventional mass continuity equation is modified to form a volumetric or bulk-continuity equation. The use of this bulk-continuity relation allows the hydrodynamic variables to be computed over the entire flow domain including both liquid and gas regions. Thus, the free-surface boundary conditions are imposed implicitly and the problem formulation is greatly simplified. The numerical procedure is validated by comparing the predicted results of a periodic standing waves problems with analytic solutions or experimental results from the literature. The results show that this numerical method produces accurate and physically realistic predictions of three-dimensional free-surface flows.