• Journal of Fisheries and Marine Sciences Education
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• 제26권2호
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• pp.345-356
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• 2014

The purpose of this study was to identify the knowledge level of human papilloma virus and performance of anti-cervical cancer vaccine among health college student in Korea. A quantitative, descriptive design was used to study 471 students in Ulsan, recruited from April 1 to May 30, 2011. The data were analyzed SPSS program, using descriptive statistics, t-test and ANOVA. The percentage of the participants who received HPV vaccination was 4.0%. The average level of health belief and knowledge about human papilloma virus were 3.05, 5.01 points. Therefore, it is needed to reduce HPV infection through concrete educational programs and advertisement. Also, this educational programs need to include strategies the knowledge of human papilloma virus.

• Journal of fish pathology
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• 제11권2호
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• pp.89-96
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• 1998

Marine birnavirus (MABV) has wide host range in marine organisms. To clarify various infection properties of MABV in different host species, in vitro study was performed by subculture for 10 passages in several fish cell lines. In CHSE-214, RTG-2 and RSBK-2 cells, the virus produced high yield of virus. Typical CPE with high protein expression was observed in these cells. On the contrary, the virus grown in EPC, FHM and BF-2 cells exhibited no CPE appearance although virus protein was detected. In EPC and FHM cells, the virus titer increased in later passages. The plaque size was distinctly bigger in CHSE-214, RTG-2 and RSBK-2 cells than in other cell lines. The nucleotide sequence of VP2/NS junction region on genome segment A exhibited one specific nucleotide change at 195. The different infection properties in several cell types performed in the present work might reflect in vivo MABV infection in various host species occurring in natural conditions.

• Journal of fish pathology
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• 제17권1호
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• pp.1-10
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• 2004

In order to analyse the detection of viral hemorrhagic septicemia virus (VHSV) in marine environment surrounding coastal region of Eastern and Southern sea of Korea, the pools of each organ sample of three fish were taken for virus assay from February to May in 2003. The samples comprised 42, taken from 9 species of marine fishes. The VHSV was detected from chub mackerel Scomber japonicus and striped mullet Mugil cephalus in epithelioma papulosum cyprini (EPC) cells. The identity of the virus was confirmed a reverse transcriptase-polymerase chain reaction (RT-PCR). VHSV has previously been reported from chub mackerel, but not from striped mullet. The new isolates was classified as a member of genogroup I (American type) of VHSV and was closely related to the VHSV KVHS'01-l based on comparisons of the partial nucleotide sequence of the glycoprotein (G) gene.

• Asian Journal for Public Opinion Research
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• 제10권4호
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• pp.316-339
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• 2022

The effects of the COVID-19 pandemic across various sectors are far-reaching, and the education sector is not an exemption. Approved and recognized vaccines are clinically proven to provide protection and have been considered one of the primary mitigations against the virus. Knowledge, attitude, and related practices (henceforth KAP) toward the virus is widely considered to be an essential cognitive key in public health for health intervention, prevention, and promotion. Previous studies revealed a positive correlation between knowledge and attitude. This study investigated the KAP and their relationship among the 857 college students in one state college in the Philippines using an online survey. Using descriptive statistics, the findings showed that the majority of the respondents have been fully vaccinated against COVID-19. Respondents were shown to be knowledgeable about COVID-19, specifically about its transmission, clinical symptoms, treatment, risk groups, isolation, prevention, and control. Positive attitudes toward individual and governmental mitigation and control of the virus were shown. Their scores on practices revealed that they always exhibit protective and preventive behavioral practices. Pearson correlation revealed a weak significant relationship between knowledge and practice, and a fair significant relationship between attitude and practice. No significant relationship is found between knowledge and attitude. This study hopes to find its place in the literature worthy of consideration as empirical support toward understanding a specific group's perceptive risk and preventive behavior toward the virus, policy making, and health intervention and promotion.

• Journal of fish pathology
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• 제22권3호
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• pp.229-237
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• 2009

The causative agent for the tunic softness syndrome of the cultured ascidian Halocynthia roretzi from Jan 1999 to Feb 2009 was identified using virus isolation and polymerase chain reaction (PCR). The pathogenicity of the isolated virus MABV UR-1 strain was determined by experimental infection trials. The cytopathic effects was observed in CHSE-214 cell line at a level 5.1% (4/78) in normal ascidian and 1.8% in abnormal ascidian showing tunic softness syndrome signs. MABV gene was detected in 16.8% (18/107) of normal and 13.1% (5/38) of abnormal organisms by PCR. The ratio of MABV isolation and gene detection was similar level in normal and soft tunic diseased ascidian. Based on the VP2/NS junction region sequences, eight strains of virus isolated from ascidian, were included in the same genogroup with MABV which is originally isolated in wide ranges of marine fish and shellfish species. The UR-1 strain caused 60% mortality (36.5% mortality in control group) by immersion infection and 37% mortality (same mortality in control group) in injection infection indicating no significant differences in infected and control groups. These results suggest that ascidian can act as reservoir of the MABV, and this virus is not directly related with the ascidian mortality.

• Journal of fish pathology
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• 제31권2호
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• pp.71-79
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• 2018

Wild walleye pollock were caught from Goseong, The East Sea of Korea and examined for the existence of several fish pathogenic viruses; viral hemorrhagic septicemia virus (VHSV), nervous necrosis virus (NNV) and marine birnavirus (MABV). We collected 1,253 wild walleye pollock in total during February 2015 and August 2018. 324 spleen sample sets and 259 brain sample sets were made, and examined for the existence of the viruses mentioned above by reverse transcriptase polymerase chain reaction (RT-PCR). None of the target viruses were detected by one-step PCR. When some of these samples were further examined by two-step PCR, 19.7% (36/183) of spleen sample sets were positive for VHSV, and 4.4% (8/183) of spleen sample sets and 1.2% (3/259) of brain sample sets were positive for NNV. The target sequences of these viruses were clustered with those previously reported in Korea (Genotype IVa of VHSV, RGNNV genotype of NNV) by phylogenetic analysis. The activity of these viruses are not clear because virus isolation was not attempted, but probably very low because all the positive samples were detected by two-step PCR.

• Journal of fish pathology
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• 제13권1호
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• pp.53-59
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• 2000

Presence of marine birnavirus (MABV) was examined against egg and ovarian fluid, and seminal fluid from the brood stocks of flounder, Paralichthys olivaceus collected from 9 different stations around Korean peninsula. The detection rate of MABV in brood stocks flounder was observed to 34% by PCR. The mean virus titer of the PCR positive fish was $10^{2.30}$ to $10^{4.30}$ $TCID_{50}$/g(ml). By a neutralization test, all of the isolated virus were ascertained to be closely related to marine birnavirus (MABV).

• BMB Reports
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• 제41권9호
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• pp.670-677
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• 2008

A cDNA microarray composed of 2,028 different ESTs from two shrimp species, Penaeus monodon and Masupenaeus japonicus, was employed to identify yellow head virus (YHV)-responsive genes in hemocytes of P. monodon. A total of 105 differentially expressed genes were identified and grouped into five different clusters according to their expression patterns. One of these clusters, which comprised five genes including cathepsin L-like cysteine peptidase, hypothetical proteins and unknown genes, was of particular interest because the transcripts increased rapidly ($\leq$ 0.25 hours) and reached high expression levels in response to YHV injection. Microarray data were validated by realtime RT-PCR analyses of selected differentially expressed transcripts. In addition, comparative analysis of the hemocyte transcription levels of three of these genes between surviving and non-surviving shrimp revealed significantly higher expression levels in surviving shrimp.

• Journal of fish pathology
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• 제31권1호
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• pp.49-55
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• 2018

Tendency of viral hemorrhagic septicemia virus (VHSV) detection from 2001 to 2016 in Korea was studied based on 15 reported cases. Since the VHSV was first detected from cultured olive flounder (Paralichthys olivaceus) in Pohang in 2001, it has been continuously reported from olive flounder farms in various regions of the Korean coastal area. So far, the virus has been detected from 2 farmed fishes, 12 wild marine fishes and 2 marine bivalves. All the 67 isolates were belong to VHSV genotype IVa. The predisposing factor analysis from different olive flounder farms revealed that the VHSV were highly detected from the juveniles under 40 g in body weight, in the temperature range from 9.5 to $17^{\circ}C$ and during the period of March to June. Therefore, we recommend that farmers, need to exercise caution against VHSV infection in Spring.

• Korean Journal of Environmental Biology
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• 제37권4호
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• pp.474-482
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• 2019

Disease monitoring in wild aquatic animals is necessary to obtain information about disease occurrence, disease agents, and the transmission of diseases between wild and cultured species. In this study, we monitored viral diseases in wild marine fish and crustacea caught by trawl in Korea in April and October 2018. We monitored the viral diseases in 977 fish from 39 different species and 287 crustacea from 14 different species. In fish, we collected kidney and spleen to detect viral hemorrhagic septicemia virus (VHSV), red sea bream iridovirus (RSIV), marine birnavirus (MABV), hirame rhabdovirus (HRV), and lymphocystis disease virus (LCDV). In crustacea, we monitored white spot syndrome virus (WSSV), infectious hypodermal and hematopoietic necrosis virus (IHHNV), taura syndrome virus (TSV), infectious myonecrosis virus (IMNV), yellowhead disease virus (YHDV), and white tail disease virus (WTDV) using pleopods, pereiopods, gills, muscle, and hepatopancreases. Although none of the viral diseases tested in this study were detected in the samples, these results will help disease control between aquaculture species and wild aquatic animals.