Journal of fish pathology (한국어병학회지)

The Korean Society of Fish Pathology (한국어병학회)
권호 표지

Isolation of marine birnavirus from ascidian Halocynthia roretzi, and its relation with tunic softness syndrome

멍게, Halocynthia roretzi에서 분리된 해양버나바이러스의 특성과 물렁증과의 관련성

  • Song, Jin-Kyung (Department of Aqualife Medicine, College of Fisheries and Ocean Science, Chonnam National University) ;
  • Yun, Hyun-Mi (Department of Aqualife Medicine, College of Fisheries and Ocean Science, Chonnam National University) ;
  • Choi, Byeong-Dae (Department of Seafood Science and Technology/Institutue of Marine Industry, Gyeongsang National University) ;
  • Oh, Myung-Joo (Department of Aqualife Medicine, College of Fisheries and Ocean Science, Chonnam National University) ;
  • Jung, Sung-Ju (Department of Aqualife Medicine, College of Fisheries and Ocean Science, Chonnam National University)
  • 송진경 (전남대학교 수산해양대학 수산생명의학과) ;
  • 윤현미 (전남대학교 수산해양대학 수산생명의학과) ;
  • 최병대 (경상대학교 해양과학대학 해양식품공학과/해양산업연구소) ;
  • 오명주 (전남대학교 수산해양대학 수산생명의학과) ;
  • 정성주 (전남대학교 수산해양대학 수산생명의학과)
  • Published : 2009.12.01
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Abstract

The causative agent for the tunic softness syndrome of the cultured ascidian Halocynthia roretzi from Jan 1999 to Feb 2009 was identified using virus isolation and polymerase chain reaction (PCR). The pathogenicity of the isolated virus MABV UR-1 strain was determined by experimental infection trials. The cytopathic effects was observed in CHSE-214 cell line at a level 5.1% (4/78) in normal ascidian and 1.8% in abnormal ascidian showing tunic softness syndrome signs. MABV gene was detected in 16.8% (18/107) of normal and 13.1% (5/38) of abnormal organisms by PCR. The ratio of MABV isolation and gene detection was similar level in normal and soft tunic diseased ascidian. Based on the VP2/NS junction region sequences, eight strains of virus isolated from ascidian, were included in the same genogroup with MABV which is originally isolated in wide ranges of marine fish and shellfish species. The UR-1 strain caused 60% mortality (36.5% mortality in control group) by immersion infection and 37% mortality (same mortality in control group) in injection infection indicating no significant differences in infected and control groups. These results suggest that ascidian can act as reservoir of the MABV, and this virus is not directly related with the ascidian mortality.

Keywords

References

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