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http://dx.doi.org/10.7847/jfp.2018.31.2.071

Monitoring of viruses in wild walleye pollock (Gadus chalcogrammus) population in Korea  

Seo, Hyun-Joon (Department of Marine Bioscience, Gangneung-Wonju National University)
Nam, U-Hwa (The East Coast Research Institute of Life Science, Gangneung-Wonju National University)
Kim, Jeong-Ho (Department of Marine Bioscience, Gangneung-Wonju National University)
Publication Information
Journal of fish pathology / v.31, no.2, 2018 , pp. 71-79 More about this Journal
Abstract
Wild walleye pollock were caught from Goseong, The East Sea of Korea and examined for the existence of several fish pathogenic viruses; viral hemorrhagic septicemia virus (VHSV), nervous necrosis virus (NNV) and marine birnavirus (MABV). We collected 1,253 wild walleye pollock in total during February 2015 and August 2018. 324 spleen sample sets and 259 brain sample sets were made, and examined for the existence of the viruses mentioned above by reverse transcriptase polymerase chain reaction (RT-PCR). None of the target viruses were detected by one-step PCR. When some of these samples were further examined by two-step PCR, 19.7% (36/183) of spleen sample sets were positive for VHSV, and 4.4% (8/183) of spleen sample sets and 1.2% (3/259) of brain sample sets were positive for NNV. The target sequences of these viruses were clustered with those previously reported in Korea (Genotype IVa of VHSV, RGNNV genotype of NNV) by phylogenetic analysis. The activity of these viruses are not clear because virus isolation was not attempted, but probably very low because all the positive samples were detected by two-step PCR.
Keywords
wild walleye pollock; Gadus chalcogrammus; viral hemorrhagic septicemia virus; nervous necrosis virus; RT-PCR;
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1 Meyers, T.R., Short, S. and Lipson, K.: Isolation of the north American strain of viral hemorrhagic septicemia virus (VHSV) associated with epizootic mortality in two new host species of Alaskan marine fish. Dis. Aquat. Org., 38: 81-86, 1999.   DOI
2 Nylund, A., Karlsbakk, E., Nylund, S., Isaksen, T.E., Karlsen, M., Korsnes, K., Handeland, S., Martinsen, R., Mork Pedersen, T. and Ottem, K.F.: New clade of betanodaviruses detected in wild and farmed cod (Gadus morhua) in Norway. Archiv. Virol., 153: 541-547, 2008.   DOI
3 Seo, J.Y. and Kwon, O.N.: Naturally collection and development until yolk absorption of domestic walleye pollock Theragra chalcogramma fertilized eggs and larvae. J. Kor. Academia-Industrial Coop. Soc., 18: 49-54, 2017.   DOI
4 Snow, M., Cunningham, C.O. and Bricknell, I.R.: Susceptibility of juvenile Atlantic cod Gadus morhua to viral haemorrhagic septicaemia virus isolated from wild-caught Atlantic cod. Dis. Aquat. Org., 41: 225-229, 2000.   DOI
5 Stone, D.M., Way, K. and Dixon, P.F.: Nucleotide sequence of the glycoprotein gene of viral hemorrhagic septicaemia (VHS) viruses from different geographical areas: a link between VHS in farmed fish species and viruses isolated from North Sea cod (Gadus morhua L.). J. Gen. Virol., 78: 1319-1326, 1997.   DOI
6 Suebsing, R.: Development and evaluation of a reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay for detecting fish viruses in Korea. Ph.D. Thesis, Gangneung-Wonju national university, Gangneung, Korea, 2012.
7 Sohn, S.G., Park, M.A., Oh, M.J. and Chun, S.K.: A fish nodavirus isolated from cultured seven band grouper, Epinephelus septemfasciatus. J. Fish. Pathol., 11: 97-104, 1998.
8 Suzuki, S., Hosono, N. and Kusuda, R.: Detection of aquatic birnavirus gene from marine fish using a combination of reverse transcription- and nested PCR. J. Mar. Biotechnol., 5: 205-209, 1997.
9 Tamura, K., Stecher, G., Peterson, D., Filipski, A., and Kumar, S.: MEGA6: Molecular Evolutionary Genetics Analysis Version 6.0. Mol. Biol. Evol., 30: 2725-2729, 2013.   DOI
10 강수경, 박정호, 김수암: 1970-1990년대 동해에서 어획된 명태(Theragra chalcogramma)의 체장에 따른 체급별 어획 마릿수 추정. 한국수산과학회지, 16: 445-453, 2003.
11 김선래, 김은미: 한국의 러시아 명태 수입과 러시아 수산업투자 고찰. 한국 시베리아연구, 18: 55-78, 2014.
12 김위식, 김종오: 능성어(Epinephelus septemfasciatus) 양식장에서의 바이러스성신경괴사증(VNN) 예방 대책. 한국수산과학회지, 48: 403-410, 2015.
13 김수미, 박수일: 우리나라 연근해 자연산 해수 어종에서 Viral Hemorrhargic Septicemia Virus(VHSV)의 검출. 한국어병학회지, 17: 1-10, 2004.
14 김춘섭, 김위식, Nishizawa Toyohiko, 오명주: 능성어 양식장에서의 viral nervous necrosis (VNN) 발생 양상. 한국어병학회지, 25: 111-116, 2012.
15 남우화, 전찬혁, 서현준, 최다영, 권오남, 김위식, 김정호: 양성 중인 명태 (Gadus chalcogrammus)의 바이러스 모니터링. 한국어병학회지, 30:1-9, 2017.
16 안상중, 조미영, 지보영, 박명애: 아시아에서 분리된 viral hemorrhagic septicemia virus (VHSV) isolates의 계통분석학적 비교, 한국어병학회지, 26: 149-161, 2013.
17 양윤선, 강수경, 김수암, 김순송: 명태(Theragra chalcogramma) 이석 내 산소동위원소 조성과 서식 수온 특성. Ocean Polar Res, 30: 249-258, 2008.   DOI
18 오태기, 사쿠라모토 카즈미, 이상고: 동해안에 있어서의 명태 어획량 변동과 산란장의 수온과의 관계. 한국수산자원학회지, 6: 2-13, 2004.
19 김위식, 김시우, 오명주: 신경괴사증바이러스 (nervous necrosis virus, RGNNV genotype)에 대한 단클론 항체 생산. 한국수산과학회지 51: 328-331, 2018.
20 이월라, 윤현미, 김석렬, 정성주, 오명주: 남서해안과 동중국해 자연산 어류에서 viral hemorrhagic septicemia virus (VHSV) 검출. 한국어병학회지, 20: 201-209, 2007.
21 Hedrick, R.P.: Relationships of the hosts, pathogen and environment: Implications for diseases of cultured and wild fish populations. J. Aquat. Ani. Health., 10: 107-111, 1998.   DOI
22 Cha, S.J., Do, J.W., Lee, N.S., An, E.J., Kim, Y.C., Kim, J.W. and Park, J.W.: Phylogenetic analysis of betanodaviruses isolated from cultured fish in Korea. Dis. Aquat. Org., 77: 181-189, 2007.   DOI
23 Doan, Q.K., Vandeputte, M., Chatain, B., Morin, T. and Allal, F.: Viral encephalopathy and retinopathy in aquaculture: a review. J. Fish Dis., 40: 717-742, 2017.   DOI
24 Dixon, P.F., Avery, S., Chambers, E., Feist, S., Mandhar, H., Parry, L., Stone, D.M., Strommen, H.K., Thurlow, J.K., Lui, C.T. and Way, K.: Four years of monitoring for viral hemorrhagic septicaemia virus in marine waters around the United Kingdom. Dis. Aquat. Org., 54: 175-186, 2003.   DOI
25 Jensen, M.H.: Research on the virus of Egtved disease. Ann. NY Acad. Sci., 126: 422-426, 1965.
26 Krkosek, M.: Population biology of infectious diseases shared by wild and farmed fish. Can. J. Fish. Aquat. Sci., 74: 620-628, 2017.   DOI
27 Mao, M.G., Wen, S.H., Peralvarez-Marin, A., Li, H., Jiang, J.L., Jiang, Z.Q., Li, X., Sun, H., Lu, H.Q.: Evidence for and characterization of nervous necrosis virus infection in Pacific cod (Gadus macrocephalus). Archiv. Virol., 160: 2237-2248, 2015.   DOI
28 Meyers, T.R., Sullivan, J., Emmenegger, E., Follett, J., Short, S., Batts, W.N., Winton, J.R.: Identification of viral hemorrhagic septicemia virus isolated from Pacific cod Gadus macrocephalus in Prince William Sound, Alaska, USA. Dis. Aquat. Org., 12: 167-175, 1992.   DOI