• Journal of Radiopharmaceuticals and Molecular Probes
• /
• v.7 no.2
• /
• pp.105-111
• /
• 2021

⁸⁹Zr is a positron-emitting radioisotope, which has known as well-suited radioisotope for use in a monoclonal antibody-based imaging agent for immuno-PET. The purpose of this study was to quantitatively evaluate the diagnostic ability of general trastuzumab and thio-trastuzumab as HER2 positive receptors based on Df hexadentate iron chelator. Desferrioxamine-p-SCN (Df-Bz-NCS) and desferroixamine-maleimide (Df-Mal) were purchased from Macrocyclics (Dallas, TX, USA). The trastuzumab was purchased from Roche (Schweiz), and thio-trastuzumab was obtained from professor Hyo-Jeong Hong group (Kangwon National University). The radioisotope ⁸⁹Zr was produced by domestic purification system and KIRAMS using medical cyclotron (50 MeV, Scantronix). The conjugates of Df-trastuzumab and Df-thio-trastuzumab were prepared with Df-Bz-NCS and Df-Mal under basic aqueous solution (pH 8-9) at room temperature, respectively. The conjugates purified by PD-10 column were mixed with dried ⁸⁹Zr chloride. ⁸⁹Zr-labeled conjugates were purified and concentrated by Amicon ultra centrifugal filter. The preparation step and time of ⁸⁹Zr-labeled conjugates was shorted as 4 steps within 2 hours. ⁸⁹Zr-labeled conjugates showed the highly radiochemical purity of over 98%, and were very stable until 7 days by the analysis of radio-ITLC method. Each radio-labeled conjugates were also exhibited the highly stability in both PBS buffer and mouse serum. Immuno-PET imaging of ⁸⁹Zr-labeled conjugates in mice bearing gastric cancer xenograft tumors with HER2 expression showed high tumor uptake in the NCI-N87 HER2-expressing. However, ⁸⁹Zr-Df-Mal-thio-trastuzumab showed a relatively lower tumor-to-background ratio than ⁸⁹Zr-Df-Bz-trastuzumab, as well as whole-body distribution. In the results, ⁸⁹Zr-Df-Bz-trastuzumab was evaluated to have a relatively higher HER2 diagnostic ability than ⁸⁹Zr-Df-Mal-thio-trastuzumab.

• Journal of Apiculture
• /
• v.32 no.3
• /
• pp.147-154
• /
• 2017

Honeybees (Apis mellifera) are common pollinators and important insects studied in agriculture, ecology and basic research. Recently, RDA (Rural Development Administration) and YIRI (Yecheon-gun Industrial Insect Research Institute) have been breeding a triple crossbred honey bee named Jangwon, which have the ability to produce superior quality honey. In this study, we identified a single nucleotide polymorphism (SNP) marker in the genome of Jangwon honeybee, particularly, in the paternal line (D line). Initially, we performed Sequence-Based Genotyping (SBG) using the Illumina Hiseq 2500 in 5 honeybee inbred lines; A, C, D, E, and F; and obtained 1,029 SNPs. Seventeen SNPs for each inbred line were generated and selected after further filtering of the SNP dataset. The 17 SNP markers validated by performing TaqMan probe-based real-time PCR and genotyping analysis was conducted. Genotyping analysis of the 5 honeybee inbred lines and one hybrid line, $D{\times}F$, revealed that one set of SNP marker, AmD9, precisely discriminated the inbred line D from the others. Our results suggest that the identified SNP marker, AmD9, is successful in distinguishing the inbred honeybee lines D, and can be directly used for genotyping and breeding applications.

• Journal of Engineering Education Research
• /
• v.10 no.3
• /
• pp.21-37
• /
• 2007

The purpose of this study is to exploring the direction of woman engineering education by the analysis of learner's recognition. In order to investigate the direction of woman engineering education, the literature reviews were explored in the context of the human resource developmentand in the viewpoint of instructional technology. The survey results such as the learner's experience recognition of engineering education were analyzed and they were discussed by experts in the field of education, instructional technology, and engineering. From the analysis result of 399 students(man:206, woman:193) in P university, there were significant differences with man and woman to the factors of (a) understanding, (b) satisfaction, (c) motivation, (d) learning ability, (e) parents' expectation, (f) pleasure in the study, and (g) expectation grade. This study was suggesting the recommendations of woman engineering education in the viewpoints of cognition, emotion, motivation, environment and instructional strategy. The research results will show the cues of human resource development for women in the field of engineering education.

• Journal of the Korean Society of Food Science and Nutrition
• /
• v.36 no.9
• /
• pp.1198-1204
• /
• 2007

This study was carried out to find the optimal conditions for the extraction of the effective ingredients based on central composite by monitoring the extraction characteristics of each ingredient with a response surface methodology. The optimal condition for the effective component alliin was extract temperature of $60.86^{\circ}C$, extract time of 3.77 hr, and ethanol concentration of 50.68%, and that for allicin was $65.11^{\circ}C$, 2.79 hr, and 13.62%, respectively, with the maximum extraction of 16.72 mg%. The maximum value of extracted total phenolics was 16.72 mg%, the optimal condition for electron donating ability was $93.35^{\circ}C$, 3.22 hr and 10.38%. The optimal conditions for pH 1.2 and 3.0 nitrite-scavenging ability was extract temperature of $79.77^{\circ}C$ and $76.46^{\circ}C$, extract time of 3.22 hr and 3.31 hr, and the ethanol concentration of 10.38% and 1.12%, respectively. With this optimal condition, the obtained maximum values for nitrite-scavenging activities at pH 1.2 and 3.0 were 94.85% and 63.22%, respectively.

• Korean Journal of Plant Resources
• /
• v.34 no.4
• /
• pp.278-286
• /
• 2021

Efficient in vitro regeneration system is essential for the successful crop breeding of soybean (Glycine max (L.) Merr.) using the new biotechnology. The genotype of donor plants strongly influences the establishment of tissue culture system. Therefore, the screening of genotypes with excellent tissue culture ability is very important for soybean genetic improvement. In this study, we report the tissue culture efficiency of 21 soybean cultivars belong to Korean soybean core-collection and two foreign cultivars (Jack and Maverick). The Kwangan, Anpyeong and Seonam are share close genetic relationship in 21 cultivars and these three cultivars were observed the high frequency of germination and regeneration. Furthermore, the high tissue culture abilities were also observed in the Williams 82 used in reference genome sequencing and the two foreign cultivars. The transformation of pBAtc:tRNA with bar gene was performed by Agrobacterium tumefaciens in the cultivars with high tissue culture ability. Transformation of the bar gene was identified by PCR analysis in Kwangan, Pungwon, Seonam, and Maverick. Our results provide useful information for the breeding of various soybean cultivars by plant biotechnology such as, genome editing.

• 한국사회복지학회:학술대회논문집
• /
• 2000.04a
• /
• pp.615-628
• /
• 2000

It is said that stress is the threat of peace and excess of resources which man has from special relationship between individual and environment. This study is for suggesting the method of intervention of the clinical social work service concerning stress for the adolescents who can conceive looking into the relationship between adolescent pregnancy and stress through the sundry records for them. When the change of making into parents from pregnancy overlapped with stress in the cycle of life as an adolescent period itself, the accumulated stress grows much bigger. The repeated change causes an obscurity of individual role and worsens stress and brings confusion on a priority ranking of developmental task. The pregnancy of adolescence has a demoralizing influence upon the adolescents themselves who are going to be adults, their children, and society. These negative effects won't be end in a short period so the effort of intervention in necessary to improve the coping skills and adaptability of pregnant adolescents. In case of pregnant adolescents, the approach of personal counsel is not just enough and effective prevention program can be better alternative and purpose at the same time. This intervention protects adolescents from the distress which can be easily disclosed after delivery and contribute to promote a good mental health of adolescents who will grow to be adults later. After all it implies the necessity of stress management program which can improve the coping ability against stress and develop positive recognition for the pregnant adolescents. Therefore much profounder study for practical intervention program, implementation, and evaluation is necessary with the conclusion which has the results of this literature study.

• Journal of Yeungnam Medical Science
• /
• v.14 no.1
• /
• pp.183-196
• /
• 1997

An experimental study was performed to investigate. The subjects drank (0.5g/kg ethanol and performed 7 items of SPES(simple reaction time, color word stress, digit classification, finger tapping speed, numerical ability, symbol digit coding, memory digit span). 20 students of medical college participated in the study during August, 1996. After ethanol intake, performance of 4 items(simple reaction time, digit classification, finger tapping speed, symbol digit coding) significantly showed to be decreased. The function of perception-response speed and steady movement were found to be more sensitive to ethanol than that of short-term memory, numerical ability and specification of color. No significant association were found between smoking, alcohol drinking, BMI(body mass index) and the effects of ethanol on neurobehavioral performance.

• Journal of the Korean Society of Food Science and Nutrition
• /
• v.34 no.3
• /
• pp.323-329
• /
• 2005

This study was conducted to investigate the effect of mycelia extracts of mushroom-cultured ginseng by-product on electron donating ability and proliferation of hepatic cancer cell (Hep3B) lines and sarcoma 180(S-180). The ginseng by-product was obtained from ginseng residues generated in processing of ginseng water extract. Mushroom strains used for preparation of mushroom mycelia cultured with ginseng residue were Phellinus linteus, Ganoderma lucidum, Coriolus versicolor and Lentinus edodes. The electron donating abilities of the test samples were increased in a dose-dependent manner in the range of 500ppm to 10,000ppm, and Coriolus versicolor extract showed the most potent activity among four mycelia extracts. In an anti-cancer test using Hep3B cells, ethanol extract showed higher antiproliferating effect than water extract. Ethanol extract of Lentinus edodes showed growth-inhibitory effect of 99.1% at 5,000ppm. All of mycelia extracts of mushroom showed the tumor suppressive effect in mice injected with S-180 cells. The growth­inhibitoy rates against tumor cells were 59% for Phellinus linteus, 61% for Ganoderma lucidum, 65% for Coriolus versicolor, 56% for Lentinus edodes. In conclusion, these results suggest that mycelia extracts of mushroom cultured with ginseng by-product have an antiproliferating effect against Hep3B cell and S-180 tumor cells.

• The Journal of Korean Philosophical History
• /
• no.43
• /
• pp.171-202
• /
• 2014

The purpose of this study was to explore the changes of right people for society on the period from liberation in 1945 to democracy in the late 1990s. Those periods classified into 'Transitional Era', Industrial Era and 'Democracy Era' based on historical events significantly influenced the changes of value system of Korean people or Korean society. In this study, not only main values and right people for society but also the way of changing value system in those periods were suggested by investigating factors of politics, economy and society. The results are as follows : Main values and right people for society on each period were as follows : In Transitional Era, main values were manners and social norms, and right people for this period were practical intellectuals pursuing the principle of action-oriented truth-and- knowledge-seeking. In Industrial Era, main values were sincerity, diligence, self-help and collaboration, and right people for this period were skilled man having occupational ability which was essential for economic development. In Democracy Era, main values were sincerity, participation, equality and coexistence, and right people for this period were scientific talented man who can make the survival of the country and prosperity in the crisis of the slowdown in economic growth A discussion of right people for each era, proposed that emphasis on the pragmatic and practical type of person in those days required, On the other hand, emphasized that were considered important of Community value.

• Applied Chemistry for Engineering
• /
• v.16 no.3
• /
• pp.385-390
• /
• 2005

In this study, nano sized colloidal Ag was made by using a seed sol method. Colloidal Ag solution was spouted on the surface of the inorganic pigment using the hybridizer system and spray nozzle. Then, the surface of the inorganic pigment was modified by $TiO_2$ to obtain the antibacterial ability. In the manufacturing process of nano sized colloidal Ag, it was confirmed that the size of particles increased by addition of $AgNO_3$ and increased the reaction time. The antibacterial measurement of the inorganic pigment showed that the growth of fungus decreased as the reaction time was increased. After the antibacterial ability appeared, in 5~7 h of the antibacterial inoculation experiment, it was measured that the antibacterial activity was excellent at a fixed time frame. The photodecomposition of benzene using $TiO_2$ as the photocatalyst showed 60~70% efficiency in about 80 min. reaction time. It was shown that more than 90% of this efficiency was achieved in the reaction time of about 30 min.