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http://dx.doi.org/10.3746/jkfn.2005.34.3.323

Effect of Mycelia Extracts of Mushroom-Cultured Ginseng By-product on Proliferation in Cancer Cell Lines  

Park, Eun-Mi (Efficacy and Safety Research Center for Traditional Oriental Medicine, Daegu Hanny University, Kyongbuk Technopark)
Kim, Soo-Jung (Efficacy and Safety Research Center for Traditional Oriental Medicine, Daegu Hanny University, Kyongbuk Technopark)
Ye, Eun-Ju (Efficacy and Safety Research Center for Traditional Oriental Medicine, Daegu Hanny University, Kyongbuk Technopark)
Bae, Man-Jong (Dept. of Oriental Medicine Biofood Science, Daegu Haany University)
Jo, Kyeong-Cheol (Korea Bio Ginseng)
Publication Information
Journal of the Korean Society of Food Science and Nutrition / v.34, no.3, 2005 , pp. 323-329 More about this Journal
Abstract
This study was conducted to investigate the effect of mycelia extracts of mushroom-cultured ginseng by-product on electron donating ability and proliferation of hepatic cancer cell (Hep3B) lines and sarcoma 180(S-180). The ginseng by-product was obtained from ginseng residues generated in processing of ginseng water extract. Mushroom strains used for preparation of mushroom mycelia cultured with ginseng residue were Phellinus linteus, Ganoderma lucidum, Coriolus versicolor and Lentinus edodes. The electron donating abilities of the test samples were increased in a dose-dependent manner in the range of 500ppm to 10,000ppm, and Coriolus versicolor extract showed the most potent activity among four mycelia extracts. In an anti-cancer test using Hep3B cells, ethanol extract showed higher antiproliferating effect than water extract. Ethanol extract of Lentinus edodes showed growth-inhibitory effect of 99.1% at 5,000ppm. All of mycelia extracts of mushroom showed the tumor suppressive effect in mice injected with S-180 cells. The growth­inhibitoy rates against tumor cells were 59% for Phellinus linteus, 61% for Ganoderma lucidum, 65% for Coriolus versicolor, 56% for Lentinus edodes. In conclusion, these results suggest that mycelia extracts of mushroom cultured with ginseng by-product have an antiproliferating effect against Hep3B cell and S-180 tumor cells.
Keywords
mycelia extracts; ginseng by-product; electron donating ability; hepatic cancer cell (Hep3B); sarcoma-180;
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1 Kim KH, Kim HJ, Joo JW, Lee SR, Kim DS, Maeng WJ. 1994. Effect of substitution level of ginseng meal for alfalfa hay on the ruminal fermentation characteristics in vitro. Kor J Anim Nutr Feed 18: 481-490
2 Ota SS. 1984. Lentinus edodes. New Food Industry 26: 49-54
3 Lee BW. Lee MS, Park KM. Kim CH. Ahn PU, Choi CU. 1992. Anticancer activities of the extract from the mycelia of Coriolus versicolor. Korean J Appl Microbial Biotechnol 20: 311 315
4 Blois MS. 1958. Antioxidant determination by the use of a stable free radical. Nature 26: 1198
5 Ju HK, Lee KU, Choi BK, Bak MY, Hong SP. 1975. A study on the nutritive effect of ginseng meal in laying hen. Korean J Food Sci Technol 7: 11-14
6 Chihara G, Hamuro J, :vIaeda Y, Arai Y, Fukuoka F. 1970. Fractionation and purification of the polysaccharides with marked antitumor activity, especially lentinan from Lentinus edodes (Berk.) Sing (an edible mushroom). Cancer Res 30: 2776-2781
7 Hu SY. 1976. The genus Panax (ginseng) in Chinese medicine. Economic Botany 30: 11-28   DOI
8 Lee MK, Park H. 1987. Free amino acids of xylene-pith in Panax ginseng. Korean J Ginseng Sci 11: 32-38
9 Choi KJ, Kim MW, Kim DH. 1985. Studies on the lipid components of various ginsengs. Korean J Ginseng Sci 9: 193-203
10 Ko SR, Choi KJ, Kim HK, Han KW. 1996. Comparison of proximate composition, mineral nutrient, amino acid and free sugar contents of several panax species. Korean J Ginseng Sci 20: 36-41
11 Wang, ZG. 1995. Ginseng research of China during the past 20 year. Korean J Ginseng Sci 19: 295-317
12 Joo HK, Cho GS. 1984. A study on the production of single cell protein from ginseng-cake extract using Saccharomyces cerevisiae. Korean J Appl Microbiol Bioeng 12: 203-209
13 Lee IS, Yang EJ, Jeong YJ, Seo JH. 1999. Fermentation process and physiochemical characteristics of Yakju (Korean cleared rice wine) with addition of ginseng power. Korean J Postharvest Sci Technol 6: 463-468
14 Kim GJ, Kim HS, Chung SY. 1992. Effect of varied mushroom on lipid compositions in dietary hypercholestrolemic rats. J Korean Soc Food Nutr 21: 131-135
15 Kim HR, Shim MJ, Kim HW, Lee CO, Choi EC, Kim BK. 1984. Antitumor components extracted from the cultured mycelia of Lyophyllum decastes. Kor J Phamacogn 15: 61-73
16 Yanmaguchi M, Yearul KA. 1987. Effect of shitake and maitake mushroom on blood pressure and plasma lipids of spontaneouly hypertensive rats. J Nutr Sci Vitaminol 33: 341-345   DOI
17 Park MH, Oh KY, Lee BW. 1998. Anti-cancer activity of Letinus edodes and Pleurotus. Korean J Food Sci Technol 30: 702-708
18 Lee JW, Bang KW. 2001. Biological activity of Phellinus spp. Food Industry and Nutrition 6: 25-33
19 Choi JH, Oh SK. 1983. Studies on the anti-aging action of Korean ginseng. Korean J Food Nutr 12: 323-335
20 Jo SG. 1995. Experimental studies on the change of cytotoxic and antitumor effects according to the prebrewed method of Semen tiglii and Rhieoma coptidis. J Kor Oriental Oncology 1: 191-211
21 Jung IC, Park S, Park KS, Ha HC, Kim SH, Kim YI, Lee JS. 1996. Antioxidative effect of fruit body and and mycelial extracts of Pleurotus ostreatus. Korean J Food Sci Technol 28: 464-469
22 Jung ME, Ham SS, Nam SM, Kang IJ, Kim SJ, Chung CK. 2001. Biochemical and histological effects of Phellinus linteus methanol extract on liver lipid metabolism of rats fed $CCl_4$ and high fat. J Korean Soc Food Sci Nutr 30: 331-337
23 Kim BK, Choi EC, Kim BK. 1979. Studies on the constituents of higher fungi of Korea (XXIV). Antineoplastic actives of Coriolus versicolor (Lex Fr) Qule. Pleurotus ostreatus (Fr.) Kummer and Lentinus edodes (Berk) Sing. Arch Pharm Res 2: 145-151   DOI
24 Kang CY, Shim MJ, Choi EC, Lee UN, Kim BK. 1981. Studies on antitumor components of Korean basidiomycetes. Mycelial culture and antitumor components of Ganoderma lucidum. J Kor Biochem 14: 100-109
25 Hartwell JL. 1971. Plants used against cancer. A Suvey Lloyda 34: 386-389
26 Tsang KW, Lam CL, Lam WK. 2003. Coriolus versicolor polysaccharide peptide slows progression of advenced non small cell lung cancer. Respiratory Medicine 97: 618-624   DOI   ScienceOn
27 Choi MY, Jung TY, Hahm KJ. 1995. Cytotoxic effect of hot water soluble polysaccharides from mushroom, Lentinus edodes and vitamin A & E supplementation against $P_{388}$ cells. Korean J Nutr 28: 1091-1099
28 Tamura Y, Niinobe M, Arima T, Okuda H, Fujii S. 1973. Studies on arninopeptidases in rat liver and plasma. Biochim Biophys Acta 327: 437-445   DOI   ScienceOn
29 Li XY, Wang JF, Zhu PP. 1990. Immune enhancement of a polysaccharide peptides isolated from Coriolus versicolor. Zhobgguo Yao Li Xue Bao 11: 542-545
30 Choi JH, Ha TM, Kim YH, Rho YD. 1996. Studies on the main factors affecting the mycelial growth of Phellinus linteus. Kor J Mycol 24: 214-222
31 Cho SM, Yu SH, Shin GC. 1996. Biological activities of culture broth of some wood rotting basidiomycetes. Korean J Mycol 24: 17 24