• Mycobiology
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• v.37 no.4
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• pp.313-316
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• 2009

To determine the optimal medium conditions for the detection of the cellulolytic activity in Ganoderma lucidum, we varied three media conditions: dye reagent, pH, and temperature. First, we evaluated the use of four dyes, Congo Red, Phenol Red, Remazol Brilliant Blue, and Trypan Blue. To observe the effect of pH on the chromogenic reaction, we also made and tested various media spanning acidic and alkaline pHs, ranging from 4.5 to 8.0. Furthermore, in order to research the effect of temperature on the clear zone and the fungus growing zone, we tested temperatures ranging from 15 to $35{^{\circ}C}$. On the whole, the best protocol called for Ganoderma lucidum transfer onto media containing Congo red with pH adjusted to 7.0, followed by incubation at $25{^{\circ}C}$ for 5 days. Our results will be useful to researchers who aim to study extracellular enzyme activity in Ganoderma lucidum.

• Food Science and Biotechnology
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• v.27 no.6
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• pp.1857-1864
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• 2018

Phthalate plasticizers residue in food is a serious threat to public health. Spores of Ganoderma lucidum are easy to be contaminated with phthalates during collection and processing. In this study, supercritical fluid extraction (SFE) was performed to remove phthalates in spores of G. lucidum, and the effects on acid and peroxide values of spores' oil were also evaluated. The results showed SFE removed 100% of the residual di-iso-butyl phthalate, di-n-butyl phthalate and di-2-ethylhexyl phthalate in the spores of G. lucidum. No significant differences in polysaccharides content and fatty acid composition were observed between SFE and control spores. However, the triterpenoid extracts of SFE spores had a 7.45% increase, significantly higher than that in control spores. Accelerated oxidation tests further implied that SFE could improve the stability of spores' oil. Our results suggested SFE is a potential approach to remove phthalate from food related products.

• Journal of Practical Agriculture & Fisheries Research
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• v.9 no.1
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• pp.105-117
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• 2007

This study was carried to analyze the demonstrational cultivation process of selenium supplemented P. linteus and G. lucidum from 2005 to 2006 and ifs effect at Hochimin Agricultural Technology College(HATC) in Vietnam, according to the mutual contract between the Yunjung-nongwon, a fanning company in Korea and HATC. As a result of the demonstrational cultivation of selenium supplemented P. linteus and G. lucidum, the external aspect, such as size, thickness and color of the P. linteus and G. lucidum were very much high qualified comparing to the same strain P. linteus and G. lucidum generally cultivated in Korea. The major contribution factors to this high qualified mushroom product could be drawn as followings; keeping growing condition of the mushroom with precise data collection of Vietnam climate and weather, involvement of the various professors of Korea National Agricultural College(KNAC), maintenance of bio-technical security through the strain cultivation on the oak log in Korea, the Yunjung-nongwon manager's endeavor being consulted by the various professors of KNAC even to be a student of the agricultural chief executive officer(CEO) training course in KNAC and the HATC's constant cooperation to the mushroom demonstrational cultivation instead of the orchid which was originally contracted item.

• Microbiology and Biotechnology Letters
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• v.21 no.6
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• pp.520-526
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• 1993

A morphologically different form of Ganoderma lucidum was isolated from a cultivator's farm, and its optimum growth conditions were determined. A major difference in their morphology was color of fruit bodies. Fruit bodies of the mutant were white wherase those of normal Ganoderma lucidum were red. Spores of the mutant were global and mycelia were thin. Mycelial growth of this white mutant was favorable on potato sucrose agar medium, and optimum pH of the medium was 5.5.

• KSBB Journal
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• v.13 no.5
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• pp.547-553
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• 1998

For the efficient production of a new exo-polysaccharide from Ganoderma lucidum ASI 7004, the optimum conditions and methods in submerged cultivation were investigated with an airlift fermenter system. The optimum aeration rate was 2.5 Wm at the initial pH 5.0 and 28$^{\circ}C$. The increase of dissolved oxygen concentration by pure oxygen supply during cultivation did not improved the exo-polysaccaride production and the mycelial growth. The maximum exo-polysaccharide production and the mycelial growth under the optimum culture condition were obtained in media of glucose 60g/L, yeast extract 6g/L, (NH4)2HPO4 1g/L and KH2PO4 0.5g/L. Under these optimum medium and culture conditions, about 7.15g/L of exo-polysaccharide and 13.9g/L of mycelial growth were producted, respectively.

• Journal of the Korean Society of Food Science and Nutrition
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• v.20 no.1
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• pp.59-64
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• 1991

Studies comparing the relative effects of water soluble extract of Ganoderma lucidum kale juice and sodium dextrothyroxine on lipid component in liver brain and testis of hypercholeste-rolemic rats for 4 weeks. Therefore the aim of this work was to investigate for prevention in cardiovascular disease, Total cholesterol concentration in liver were significantly lower kale juice and sodium dextrothyroxine(1.25mg/kg diet) fed groups than the other groups and trigly-ceride concentration was significantly lower Ganodrma lucidum fed group than in control group;. Total cholesterol concentration in testis were lower other groups than in control group and triglyceride concentration were significantly lower cholesterol Ganoderma lucidum and kale juice groups in control group. Phospholipid concentration in testis was higher sodium dextroth-yroxine fed group thanin control group.

• Microbiology and Biotechnology Letters
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• v.32 no.3
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• pp.271-276
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• 2004

$\beta$-Immunan was proteoglycan obtained from mycelium of Ganoderma lucidum IY009. In this study, the protective effects of $\beta$-Immunan, against the CDDP induced in vitro cytotoxicity and in vivo renal toxicity, was measured. Concentration dependent cytotoxicities of CDDP in normal kidney cells (Vero, TCMK-l) were reduced by $\beta$-Immunan treatment. Increased renal toxicity factors, such as elevation of blood urea nitrogen (BUN) and serum creatinine, reduction of kidney weight and malonidialdehyde (MDA), by intraperitoneal administration of CDDP in rats was improved. These results indicated that $\beta$-Immunan have a protective effects against the CDDP induced renal toxicity, however, it needed to confirm the detailed mechanism for therapeutic effects.

• Journal of Microbiology and Biotechnology
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• v.20 no.11
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• pp.1521-1523
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• 2010

Bioassay-guided fractionation of a methanol extract of Ganoderma lucidum gave a pure cholesterol esterase inhibitor. On the basis of spectroscopic analysis and comparison with data from the literature, the structure of this compound was identified as $5{\alpha},8{\alpha}$-epidioxyergosta-6,22-dien-$3{\bet}$-ol (compound I). This compound inhibited cholesterol esterase activity with an $IC_{50}$ value of $42{\mu}M$. Lineweaver-Burk plot analysis revealed that compound I is a noncompetitive inhibitor. The findings of this study suggest that compound I may be the active principle of the hypocholesterolemic effect of Ganoderma lucidum.

• Mycobiology
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• v.41 no.1
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• pp.42-46
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• 2013

The effects of the composition of a mixture containing food waste compost (FWC), rice bran (RB), and oak sawdust (SD) on the antler-type fruiting body (FB) yield of Ganoderma lucidum were studied. Experiments were performed using 0 (control), 5, 10, 15, 20, 25, 30, 35, and 40% (w/w) FWC added to a basal growth medium consisting of 20% (w/w) RB and 80% (w/w) SD. The content of 15% FWC gave the highest FB yield ($27.0{\pm}1.3$ g/bottle), which was 44% higher than the yield ($18.6{\pm}2.8$ g/bottle) of the control treatment. However, FWC contents of 20~40% showed reduced yield (2.4~23.0 g/bottle), partly because FWC had a high Na concentration (0.6%). These results demonstrate the potential for use of FWC as a component of a growth medium for production of G. lucidum FBs.

• YAKHAK HOEJI
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• v.45 no.6
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• pp.641-649
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• 2001

In this study, the antitumor activities were investigated using $\beta$-lmmunan, a proteoglycan obtained from cultured mycelia of the IY009 strain of Ganoderma lucidum belonging to basidiomycetes. The result showed the significant effect of cytotoxicity test against murine sarcoma 180 and murine lymphocytic leukemia L1210 using immunized macrophage cultures by $\beta$-lmmunan. When intraperitoneally injected at 40 mg/kg/day daily for 10 days, $\beta$-lmmunan inhibited the growth of sarcoma 180 solid tumor in ICR mice by 88.8% (p<0.05). It was also observed that $\beta$-lmmunan increased life span by 85.2% (p<0.01) after treatment of 100 mg/kg/day in BDF1 mice bearing lymphocytic leukemia L1210. And combination therapy with cisplatin (dosage: 4 mg/kg) increased life span by 140.4% (p<0.05) after treatment of 100 mg/kg/day daily in BDF1 mice bearing lymphocytic leukemia L1210.