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http://dx.doi.org/10.4014/jmb.1006.06037

Isolation and Structure Determination of a Cholesterol Esterase Inhibitor from Ganoderma lucidum  

Kim, Shin-Duk (Department of Biological Engineering, Seokyung University)
Publication Information
Journal of Microbiology and Biotechnology / v.20, no.11, 2010 , pp. 1521-1523 More about this Journal
Abstract
Bioassay-guided fractionation of a methanol extract of Ganoderma lucidum gave a pure cholesterol esterase inhibitor. On the basis of spectroscopic analysis and comparison with data from the literature, the structure of this compound was identified as $5{\alpha},8{\alpha}$-epidioxyergosta-6,22-dien-$3{\bet}$-ol (compound I). This compound inhibited cholesterol esterase activity with an $IC_{50}$ value of $42{\mu}M$. Lineweaver-Burk plot analysis revealed that compound I is a noncompetitive inhibitor. The findings of this study suggest that compound I may be the active principle of the hypocholesterolemic effect of Ganoderma lucidum.
Keywords
Ganoderma lucidum; ergosterol peroxide; cholesterol esterase inhibitor; hypocholesterolemic effect;
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