• Biotechnology and Bioprocess Engineering:BBE
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• v.5 no.4
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• pp.234-241
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• 2000

The Saccharomyces cerevisiae PMR1 gene encodes a Ca2+-ATPase localized in the Golgi. We have investigated the effects of PMR1 disruption in S. cerevisiae on the glycosylation and secretion of three heterologous glycoproteins, human ${\alpha}$1-antitrypsin (${\alpha}$1-AT), human antithrombin III (ATHIII), and Aspergillus niger glucose oxidase (GOD). The pmr1 null mutant strain secreted larger amounts of ATHIII and GOD proteins per a unit cell mass than the wild type strain. Despite a lower growth rate of the pmr1 mutant, two-fold higher level of human ATHIII was detected in the culture supernatant from the pmr1 mutant compared to that of the wild-type strain. The pmr1 mutant strain secreted ${\alpha}$1-AT and the GOD proteins mostly as core-glycosylated forms, in contrast to the hyperglycosylated proteins secreted in the wild-type strain. Furthermore, the core-glycosylated forms secreted in the pmr1 mutant migrated slightly faster on SDS-PAGE than those secreted in the mnn9 deletion mutant and the wild type strains. Analysis of the recombinant GOD with anti-${\alpha}$1,3-mannose antibody revealed that GOD secreted in the pmr1 mutant did not have terminal ${\alpha}$1,3-linked mannose unlike those secreted in the mnn9 mutant and the wild type strains. The present results indicate that the pmr1 mutant, with the super-secretion phenotype, is useful as a host system to produce recombinant glycoproteins lacking high-mannose outer chains.

• Journal of Microbiology and Biotechnology
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• v.17 no.4
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• pp.547-559
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• 2007

Bacillus thuringiensis (Bt) was first described by Berliner [10] when he isolated a Bacillus species from the Mediterranean flour moth, Anagasta kuehniella, and named it after the province Thuringia in Germany where the infected moth was found. Although this was the first description under the name B. thuringiensis, it was not the first isolation. In 1901, a Japanese biologist, Ishiwata Shigetane, discovered a previously undescribed bacterium as the causative agent of a disease afflicting silkworms. Bt was originally considered a risk for silkworm rearing but it has become the heart of microbial insect control. The earliest commercial production began in France in 1938, under the name Sporeine [72]. A resurgence of interest in Bt has been attributed to Edward Steinhaus [105], who obtained a culture in 1942 and attracted attention to the potential of Bt through his subsequent studies. In 1956, T. Angus [3] demonstrated that the crystalline protein inclusions formed in the course of sporulation were responsible for the insecticidal action of Bt. By the early 1980's, Gonzalez et al. [48] revealed that the genes coding for crystal proteins were localized on transmissible plasmids, using a plasmid curing technique, and Schnepf and Whiteley [103] first cloned and characterized the genes coding for crystal proteins that had toxicity to larvae of the tobacco hornworm, from plasmid DNA of Bt subsp. kurstaki HD-1. This first cloning was followed quickly by the cloning of many other cry genes and eventually led to the development of Bt transgenic plants. In the 1980s, several scientists successively demonstrated that plants can be genetically engineered, and finally, Bt cotton reached the market in 1996 [104].

• Microbiology and Biotechnology Letters
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• v.34 no.3
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• pp.221-227
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• 2006

BSCX1 was an antimicrobial peptide produced by Bacillus subtilis cx1. Attempts were made to determine the location of inducing factor in the bacteriocin-sensitive cell affecting bacteriocin BSCX1 production. Mixed culture of the bacteriocin producer strain B. subtilis cx1 and its sensitive strain B. subtilis ATCC6633, increased production of bacteriocin BSCX1. The result suggested the presence of a bacteriocin inducing factor in the sensitive strain. The inducing factor was localized in the cell debris and intracellular fraction of B. subtilis ATCC6633. Bacteriocin BSCX1 inducing factor was found to be highly stable in the pH range 2.5-9.5, but inactivated within 3h over $50^{\circ}C$, and treatment with proteinase K destroyed its inducing activity, this result suggested that the inducing factor should be a proteinaceous nature.

• Journal of The Korean Association For Science Education
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• v.9 no.2
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• pp.81-87
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• 1989

In 1987 Ministry of Education published the 5th-revised version of curriculm, the main goal of which is to improve science and culture as well as to educate the students who are able to actively adapt to the 21st century's society. Howerver, the revised curriculum neglects the problems associated with the nature of and localized characteristics of science education. Therefore this research had its main objective to survey and analyze the status of science education of secondary schools in Kangwon Province and, based on the results, to develop the teaching/learning materials appropriate for science education in this province. This research is 3d-Year project and this paper is about its first year research results. The first year's main objective is to investigate and analyze the status of middle school science education, focusing on the curricular operation, science teachers, and lavoratory facilities and instruments. This research used the survey methods. The questionaires were sent to all the middle schools the number of which is 163, and 162 schools of which returned the survey questionaires. Based on the analyses of the data following conclusions were drawn. The average class size is 48.8 students. Almost all of the middle schools alocate the science class hours per week of 4,3,4 hours to first, second, third grade, respectively. However, the greater part of science teachers want 5,4,5 hours of science classes a week. Total number of science teachers exeeds the number actually needed, implicating that many science teachers are teaching non-major subjects. The lavoratory facilities and instruments are not sufficiently provided for lavoratory-based science education. Along with these conclusinons suggestions for better science educations are recommanded. Among the suggestions two are highly emphasized.They are: to increase science class hours; to use field lavoratory substituting for school lavoratory.

• Journal of Fashion Business
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• v.18 no.5
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• pp.116-133
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• 2014

This study is to research the characteristics of garment which are 'written(or described)' in fashion magazines. With examples particularly of Korea and Japan, this study aims at pointing out the similarities and differences described by modifier in oversea licensed fashion magazine. It is considered to be meaningful to provide fundamental document, which can estimate the aspects of fashion that has global features and localized characteristics. This is a follow-up of a previous study, covering its range of "Vogue Korea" and "VOGUE Japan" research document, and we researched on the materials that have been mentioned most frequently in each country from May to August of 2012. To target analyzing list of top 6 materials, we compared and analyzed the characteristics of the kind of material represented by Korean and Japanese fashion magazines and modifier's properties. As established in the previous study, to designate each contrary concept to a total of 8 groups as an analyzing frame, we researched the properties of each material and differences between Korea and Japan.

• Review of Culture and Economy
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• v.19 no.2
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• pp.147-170
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• 2016

This study has identified the current status and challenges to development of Korean Ethnic Media Companies in North America, China, and Australia New Zealand and aimed to understand implication of cultural policy. It examines the media circumstances, business resources of Korean Ethnic Media Companies(KEMC), and Korean ethnic broadcasting aid project which is operated for 10 years, and interviewed nine CEOs' of KEMC. According to the analysis, KEMCs in North America are in the market which is fully exposed to competition. China's KEMCs are the "limitedly localized market" which is supported by the government's regulatory for minority media. Australia & New Zealande's KEMCs are the market which potential growth is expected. North American KEMCs are needed to upgrade the business structure and organizational resources for content differentiation. China's KEMCs have to diversify financial resources and to consolidate content power. and Australia & New Zealande's KEMCs need support & cooperatation from home country for ensuring high-quality content.

• Archives of Hand and Microsurgery
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• v.23 no.4
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• pp.271-276
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• 2018

A 60-year-old male presented with a three-month history of redness and swelling on his left little finger. His medical history was not informative. Wound culture revealed methicillin-resistant Staphylococcus aureus. After vancomycin administration, the skin lesions became worse and whole body bullae and desquamation occurred. This was initially suspected to be a drug eruption; thus, we switched antibiotics from vancomycin to teicoplanin. However, biopsy revealed Staphylococcal scalded skin syndrome (SSSS). After several days, generalized skin symptoms improved. The patient recovered and is in good physical health without recurrence six months later. We describe a localized form of SSSS, which is very rare in healthy adults. Consequently, there is a high risk of misdiagnosis. Thus, we report a rare case of SSSS in a healthy adult and the importance of early histological examination for accurate diagnosis.

• KOMUNHWA
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• no.68
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• pp.59-75
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• 2006

At the beginning of the 21st century with taking a serious view of the harmony between human and nature, and of keeping an ecological balance, China has tried to reduce the gap in economy between rural districts and the urban city, and to carry out the policies for establishing a harmonious socialism through full-scale cooperation and continuous developments. And in order to preserve their traditional culture and to keep the historical legitimacy of People's Republic of China against urgent economical changes since the reform and openness, China has stressed the importance of training patriotic attitude on their cultural heritages. Thus the Chinese museums started to recognize more ad more the importance of social education as well as their functions of preserving the cultural property and of exhibiting it. And with them they have turned their attentions to an Ecomuseum, a new type of a museum. The ecomuseum in China was first established at a remote place between mountains where the ethnic minority inhabited. This was because of the feeling of some crises on culture native to the minority, followed by the process of China's Western Region Development. And it was recognized that they should participate actively in creating the cultural demand of the minority and in establishing a harmonious society with improving economic condition. Therefore in order to activate the ecomuseum it is localized and sinicized little by little through strengthening the management of cultural heritages of the minority.

• Journal of Veterinary Clinics
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• v.27 no.3
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• pp.232-239
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• 2010

Two cell lines derived from spontaneous canine mammary gland tumors were established and characterized. Mammary gland tumors from 9 years old pug and 9 years old toy-poodle dogs were collected by aseptic surgical resection and primary culture was performed. The histopathologic examination of tumors revealed adenocarcinoma and complex carcinoma and two dogs died from metastasis of the tumors. The tumor cells were subcultured over 60 times for more than 1 year and morphological consistency maintained. Light microscopic examination, growth curve, doubling time calculation, xenotransplantation to female nude mice, immunohistochemistry for wide spectrum keratin, vimentin, $\alpha$-smooth muscle actin and cytokeratin 8 was performed for characterization. The cell lines exhibited polygonal, elongated cell shape and cytoplasmic bridge and doubling time of 47.1 hrs and 18.6 hrs, respectively. Subcutaneous xenotransplantation to nude mice of the cells produced localized palpable mass within 4 weeks in 4 of 5 and 5 of 5 nude mice, respectively. In immunohistochemical examination one cell line showed strong positive against wide spectrum keratin and cytokeratin 8 and the other cell line showed strong positive against smooth muscle actin and cytokeratin 8. Additional characterization would be possible by investigator's needs and the cell lines may be useful for in vivo and in vitro studies of canine mammary tumor and adjuvant therapies.

• Therapeutic Science for Rehabilitation
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• v.10 no.3
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• pp.83-96
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• 2021

Objective : This study aimed to determine Korea-type family function factors that affect individual growth and social activities, and examine check the appropriate contents and domestic culture components based on the McMaster model, a family function evaluation tool, through the Delphi technique. Methods : The Delphi technique was applied to 12 expert panels in fields related to family function. The period lasted 9 weeks, from May to June 2020. The Delphi survey was conducted twice. In the first survey, the domestic culture and appropriate contents of the McMaster model were selected and localized, and expert' opinions about the components were collected using closed and open-ended questions. In the second survey, the fitness and importance of the components were investigated. Results : As a result of the first Delphi investigation, 18 items were deleted from the 53 items presented. After adding 11 items and excluding any overlapping items, a total of 40 items were selected. Subsequently, sentences that were difficult to understand were revised to familiar vocabulary. A second survey was constructed, with an example sentence. In the second Delphi investigation, 33 items were selected. The average content validity ratio for the final selected component was 0.76, and the stability was 0.28. Conclusion : Family function and the factors influencing domestic family function identified though this study can be used to conduct family function evaluations and interventions in clinical sites or relevant research studies.