Browse > Article

Establishment and Characterization of Canine Mammary Gland Cancer Cell Lines  

Lee, Sun-Tae (Department of Veterinary Surgery, College of Veterinary Medicine, Seoul National University)
Kweon, Oh-Kyeong (Department of Veterinary Surgery, College of Veterinary Medicine, Seoul National University)
Kim, Wan-Hee (Department of Veterinary Surgery, College of Veterinary Medicine, Seoul National University)
Publication Information
Journal of Veterinary Clinics / v.27, no.3, 2010 , pp. 232-239 More about this Journal
Abstract
Two cell lines derived from spontaneous canine mammary gland tumors were established and characterized. Mammary gland tumors from 9 years old pug and 9 years old toy-poodle dogs were collected by aseptic surgical resection and primary culture was performed. The histopathologic examination of tumors revealed adenocarcinoma and complex carcinoma and two dogs died from metastasis of the tumors. The tumor cells were subcultured over 60 times for more than 1 year and morphological consistency maintained. Light microscopic examination, growth curve, doubling time calculation, xenotransplantation to female nude mice, immunohistochemistry for wide spectrum keratin, vimentin, $\alpha$-smooth muscle actin and cytokeratin 8 was performed for characterization. The cell lines exhibited polygonal, elongated cell shape and cytoplasmic bridge and doubling time of 47.1 hrs and 18.6 hrs, respectively. Subcutaneous xenotransplantation to nude mice of the cells produced localized palpable mass within 4 weeks in 4 of 5 and 5 of 5 nude mice, respectively. In immunohistochemical examination one cell line showed strong positive against wide spectrum keratin and cytokeratin 8 and the other cell line showed strong positive against smooth muscle actin and cytokeratin 8. Additional characterization would be possible by investigator's needs and the cell lines may be useful for in vivo and in vitro studies of canine mammary tumor and adjuvant therapies.
Keywords
canine mammary gland tumor; cell line; establishment; characterization;
Citations & Related Records

Times Cited By SCOPUS : 0
연도 인용수 순위
  • Reference
1 Feuerhake F, Sigg W, Hofter EA, Dimpfl T, Welsch U. Immunohistochemical analysis of Bcl-2 and Bax expression in relation to cell turnover and epithelial differentiation markers in the non-lactating human mammary gland epithelium. Cell Tissue Res 2000; 299: 47-58.   DOI   ScienceOn
2 Sommers CL, Byers SW, Thompson EW, Torri JA, Gelmann EP. Differentiation state and invasiveness of human breast cancer cell lines. Breast Cancer Res Treat 1994; 31: 325-335.   DOI   ScienceOn
3 Zhou S, Schuetz JD, Bunting KD, Colapietro AM, Sampath J, Morris JJ, Lagutina I, Grosveld GC, Osawa M, Nakauchi H, Sorrentino BP. The ABC transporter Bcrp1/ABCG2 is expressed in a wide variety of stem cells and is a molecular determinant of the side-population phenotype. Nat Med 2001; 7: 1028-1034.   DOI   ScienceOn
4 Perez-martinez C, Garcia-iglesias MJ, Duran-navarrete AJ, Espinosa-alvarez J, Garcia-fernandez RA, Lorenzana-robles N, Fernandez-perez S, Garcia-marin JF. Histopathological and immunohistochemical characteristics of two canine lipid-rich mammary carcinomas. J Vet Med A Physiol Pathol Clin Med 2005; 52: 61-66.   DOI   ScienceOn
5 Destexhe E, Lespagnard L, Degeyter M, Heymann R, Coignoul F. Immunohistochemical identification of myoepithelial, epithelial, and connective tissue cells in canine mammary tumors. Vet Pathol 1993; 30: 146-154.   DOI   ScienceOn
6 Salsbury AJ. The significance of the circulating cancer cell. Cancer Treat Rev 1975; 2: 55-72.   DOI   ScienceOn
7 Sartin EA, Barnes S, Toivio-kinnucan M, Wright JC, Wolfe LG. Heterogenic properties of clonal cell lines derived from canine mammary carcinomas and sensitivity to tamoxifen and doxorubicin. Anticancer Res 1993; 13: 229-236.
8 Schneider R, Dorn CR, Taylor DO. Factors influencing canine mammary cancer development and postsurgical survival. J Natl Cancer Inst 1969; 43: 1249-1261.
9 Brodey RS. Surgical treatment of cancer in the dog. J Am Vet Med Assoc 1975; 166: 494-496.
10 Sell S, Pierce GB. Maturation arrest of stem cell differentiation is a common pathway for thecellular origin of teratocarcinomas and epithelial cancers. Lab invest 1994; 70: 6-22.
11 Taipale J, Beachy PA. The Hedgehog and Wnt signalling pathways in cancer. Nature 2001; 411: 349-354.   DOI   ScienceOn
12 Nowell PC. Mechanisms of tumor progression. Cancer Res 1986; 46: 2203-2207.
13 Fialkow PJ. Clonal origin of human tumors. Biochim Biophys Acta 1976; 458: 283-321.
14 Fearon ER, Hamilton SR, Vogelstein B. Clonal analysis of human colorectal tumors. Science 1987; 238: 193-197.   DOI
15 Rabanal RM, Else RW. Immunohistochemical localisation of cytokeratin and vimentin intermediate filament proteins in canine mammary tumours. Res Vet Sci 1994; 56: 225-233.   DOI   ScienceOn
16 Hellmen E, Lindgren A. The expression of intermediate filaments in canine mammary glands and their tumors. Vet Pathol 1989; 26: 420-428.   DOI
17 Heppner GH. Tumor heterogeneity. Cancer Res 1984; 44: 2259-2265.
18 Fidler IJ, Naito S, Pathak S. Orthotopic implantation is essential for the selection, growth and metastasis of human renal cell cancer in nude mice [corrected]. Cancer Metastasis Rev 1990; 9: 149-165.   DOI   ScienceOn
19 Ferguson HR. Canine mammary gland tumors. Vet Clin North Am Small Anim Pract 1985; 15: 501-511.   DOI
20 Campling BG, Haworth AC, Baker HM, Greer DL, Holden JJ, Bradley EC, Pym J, Dexter DF. Establishment and characterization of a panel of human lung cancer cell lines. Cancer 1992; 69: 2064-2074.   DOI   ScienceOn
21 Gazdar AF, Oie HK. Re: Growth of cell lines and clinical specimens of human non-small cell lung cancer in a serumfree defined medium. Cancer Res 1986; 46: 6011-6012.
22 Brower M, Carney DN, Oie HK, Gazdar AF, Minna JD. Growth of cell lines and clinical specimens of human nonsmall cell lung cancer in a serum-free defined medium. Cancer Res 1986; 46: 798-806.
23 Dorn CR, Taylor DO, Schenider R, Hibbard HH, Klauber MR. Survey of animal neoplasms in Alameda and Contra Costa Counties, California. II. Cancer morbidity in dogs and cats from Alameda County. J Natl Cancer Inst 1968; 40: 307-318.
24 Sorenmo KU, Shofer FS, Goldschmidt MH. Effect of spaying and timing of spaying on survival of dogs with mammary carcinoma. J Vet Intern Med 2000; 14: 266-270   DOI   ScienceOn
25 Gilbertson SR, Kurzman ID, Zachrau RE, Hurvitz AI, Black MM. Canine mammary epithelial neoplasms: biologic implications of morphologic characteristics assessed in 232 dogs. Vet Pathol 1983; 20: 127-142.   DOI
26 Fidler IJ, Hart IR. The development of biological diversity and metastatic potential in malignant neoplasms. Oncodev Biol Med 1982; 4: 161-176.