• Title/Summary/Keyword: liquid phase

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Fabrication and characterization of InGaAsP/InP multi-quantum well buried-ridge waveguide laser diodes (Buried-Ridge Waveguide Laser Diode 제작 및 특성평가)

  • 오수환;이지면;김기수;이철욱;고현성;박상기
    • Korean Journal of Optics and Photonics
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    • v.14 no.6
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    • pp.669-673
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    • 2003
  • We fabricated a buried-ridge waveguide laser diode (B-RWG LD) which has more advantages for obtaining lateral single mode operation on the same ridge width and for the planarization of the device surface, compared to the conventional RWG LD. In this LD, the difference of the lateral effective refractive index can be controlled by the thickness of the InGaAsP layer which is grown on the active and the p-InP layers. The InGaAsP multiple quantum well was grown on a n-InP substrate by the CBE. The buried ridge structure was formed by selective wet etchings, followed by liquid phase epitaxy methods. The fabricated LD with the ridge width of 7 ${\mu}{\textrm}{m}$ showed a linear increase of the optical power up to 20 ㎽ without any kinks and a saturated output power of more than 80 ㎽. By measuring the far field pattern, we demonstrate that LDs with the ridge widths of 5 ${\mu}{\textrm}{m}$ and 7 ${\mu}{\textrm}{m}$ were operated in a lateral single mode up to 2.7I$_{th}$ and 2.4I$_{th}$, respectively.ely.

Change in Physical Properties depending on Contaminants and Introduction to Case Studies of Geophysical Surveys Applied to Contaminant Detection (오염원에 따른 오염지역 물성 변화 및 물리탐사 적용 사례 소개)

  • Yu, Huieun;Kim, Bitnarae;Song, Seo Young;Cho, Sung Oh;Caesary, Desy;Nam, Myung Jin
    • Geophysics and Geophysical Exploration
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    • v.22 no.3
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    • pp.132-148
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    • 2019
  • Recently, safety and environmental concerns have become major social issues. Especially, a special underground-safety law has been made and enacted to prevent ground subsidence around construction sites. For environmental problems, several researches have started or will start on characterization of contaminated sites, in-situ environmental remediation in subsurface, and monitoring of remediation results. As a part of the researches, geophysical surveys, which have been mainly applied to explore mineral resources, geological features or ground, are used to characterize not only contaminated areas but also fluid flow paths in subsurface environments. As a basic study for the application of geophysical surveys to detect contamination in subsurface, this paper analyzes previous researches to understand changes in geophysical properties of contaminated zones by various contaminants such as leachate, heavy metals, and non-adequate phase liquid (NAPL). Furthermore, this paper briefly introduces how geophysical surveys like direct-current electrical resistivity, induced polarization and ground penetration radar surveys can be applied to detect each contamination, before analyzing case studies of the applications in contaminated areas by NAPL, leachate, heavy metal or nitrogen oxides.

Effect of Substrate Temperature and Growth Duration on Palladium Oxide Nanostructures (팔라듐 옥사이드 나노구조물의 성장에서 기판 온도와 성장 시간의 효과)

  • Kim, Jong-Il;Kim, Ki-Chul
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.20 no.4
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    • pp.458-463
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    • 2019
  • Palladium (Pd) is widely used as a catalyst and noxious gas sensing materials. Especially, various researches of Pd based hydrogen gas sensor have been studied due to the noble property, Pd can be adsorbed hydrogen up to 900 times its own volume. In this study, palladium oxide (PdO) nanostructures were grown on Si substrate ($SiO_2(300nm)/Si$) for 3 to 5 hours at $230^{\circ}C{\sim}440^{\circ}C$ using thermal chemical vapor deposition system. Pd powder (source material) was vaporized at $950^{\circ}C$ and high purity Ar gas (carrier gas) was flown with the 200 sccm. The surface morphology of as-grown PdO nanostructures were characterized by field-emission scanning electron microscopy(FE-SEM). The crystallographic properties were confirmed by Raman spectroscopy. As the results, the as-grown nanostructures exhibit PdO phase. The nano-cube structures of PdO were synthesized at specific substrate temperatures and specific growth duration. Especially, PdO nano-cube structrures were uniformly grown at $370^{\circ}C$ for growth duration of 5 hours. The PdO nano-cube structures are attributed to vapor-liquid-solid process. The nano-cube structures of PdO on graphene nanosheet can be applied to fabricate of high sensitivity hydrogen gas sensor.

Variation in Fatty Acid Composition, Caffeic and Rosmarinic Acid Content, and Antioxidant Activity of Perilla Accessions (들깨 유전자원의 지방산 변이 및 rosmarinic acid와 caffeic acid의 함량에 따른 항산화 활성 비교)

  • Kim, Da Jeong;Assefa, Awraris Derbie;Jeong, Yi Jin;Jeon, Young Ah;Lee, Jae Eun;Lee, Myeong Chul;Lee, Ho Sun;Rhee, Ju Hee;Sung, Jung Sook
    • Korean Journal of Medicinal Crop Science
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    • v.27 no.2
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    • pp.96-107
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    • 2019
  • Background: The aim of this study was to compare the fatty acid composition, antioxidant activity, and the content of two major phenolic acids, caffeic and rosmarinic acids, of 45 Perilla accessions collected from Russia. Methods and Results: A total of 45 accessions of the genus Perilla were used in this study. The antioxidant activities of these accessions were analyzed using a spectrophotometer, and their caffeic and rosmarinic acid contents were determined using a reversed-phase ultraperformance liquid chromatography (UPLC) system. The Perilla seed oil was recovered using hexane in a soxhlet extraction method. The fatty acid compositions were analyzed using a Shimadzu QP2010 gas chromatography-mass spectoroscopy system. The results showed that accessions IT226732 and IT274300 had the highest content of caffeic and rosmarinic acid, respectively. A cluster analysis based on antioxidant assay results and concentration of phenolic acids led to the categorization of Perilla accessions into three major groups. The accessions in Group I were characterized by higher caffeic and rosmarinic acid content and antioxidant activity, compared to the accessions in the other two groups (p < 0.05). The total oil content ranged between 28.39 and 46.89%. The compositions of oleic, linoleic, and linolenic acid ranged from 11.83 to 19.55%, 11.92 to 16.71%, and 59.19 to 67.28%, respectively. Conclusions: The results of this study indicated that accessions IT274300, IT226732, IT274293, IT235818, and IT235820 could be used as a source of functional materials.

Recombinant Production and Antimicrobial Activity of an Antimicrobial Model Peptide (Uu-ilys-CF) Derived from Spoon Worm Lysozyme, Uu-ilys (개불 라이소자임 유래 항균성 모델 펩타이드(Uu-ilys-CF)의 재조합 단백질 생산 및 항균 활성)

  • Oh, Hye Young;Go, Hye-Jin;Park, Nam Gyu
    • Journal of Life Science
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    • v.31 no.1
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    • pp.83-89
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    • 2021
  • Uu-ilys, an i-type lysozyme from spoon worm (Urechis unicinctus), is an innate immune factor that plays an important role in the defense against pathogens. It also possesses non-enzymatic antibacterial activity. Thus, there is a possibility to develop an antimicrobial model peptide from Uu-ilys. In this study, we report the design, production, and antibacterial activity of an Uu-ilys analog that exhibits antibacterial activity. The Uu-ilys structure was fragmented according to its secondary structures to predict the regions with antimicrobial activity using antimicrobial peptide (AMP) prediction tools from different AMP databases. A peptide containing the C-terminal fragment was predicted to exert antimicrobial activity. The chosen fragment was designated as an Uu-ilys analog containing the C-terminal fragment, Uu-ilys-CF. To examine the possibility of developing an AMP using the sequence of Uu-ilys-CF, recombinant fusion protein (TrxA-Uu-ilys-CF) was produced in an expression system that was heterologous. The produced fusion protein was cleaved after methionine leaving Uu-ilys-CF free from the fusion protein. This was then isolated through high performance liquid chromatography and reverse phase column, CapCell-Pak C18. The antibacterial activity of Uu-ilys-CF against different microbial strains (four gram-positive, six gram-negative, and one fungal strain) were assessed through the ultrasensitive radial diffusion assay (URDA). Among the bacterial strains tested, Salmonella enterica was the most susceptible. While the fungal strain tested was not susceptible to Uu-ilys-CF, broad spectrum antibacterial activity was observed.

Effect of Re and Ru Addition on the Solidification and Solute Redistribution Behaviors of Ni-Base Superalloys (니켈계 초내열합금의 응고 및 용질원소의 편석 거동에 미치는 레늄 및 루테늄 첨가의 영향)

  • Seo, Seong-Moon;Jeong, Hi-Won;Lee, Je-Hyun;Yoo, Young-Soo;Jo, Chang-Yong
    • Korean Journal of Metals and Materials
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    • v.49 no.11
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    • pp.882-892
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    • 2011
  • The influence of rhenium (Re) and ruthenium (Ru) addition on the solidification and solute redistribution behaviors in advanced experimental Ni-base superalloys has been investigated. A series of model alloys with different levels of Re and Ru were designed based on the composition of Ni-6Al-8Ta and were prepared by vacuum arc melting of pure metallic elements. In order to identify the influence of Re and Ru addition on the thermo-physical properties, differential scanning calorimetry analyses were carried out. The results showed that Re addition marginally increases the liquidus temperature of the alloy. However, the ${\gamma}^{\prime}$ solvus was significantly increased at a rate of $8.2^{\circ}C/wt.%$ by the addition of Re. Ru addition, on the other hand, displayed a much weaker effect on the thermo-physical properties or even no effect at all. The microsegregation behavior of solute elements was also quantitatively estimated by an electron probe microanalysis on a sample quenched during directional solidification of primary ${\gamma}$ with the planar solid/liquid interface. It was found that increasing the Re content gradually increases the microsegregation tendency of Re into the dendritic core and ${\gamma}^{\prime}$ forming elements, such as Al and Ta, into the interdendritic area. The strongest effect of Ru addition was found to be Re segregation. Increasing the Ru content up to 6 wt.% significantly alleviated the microsegregation of Re, which resulted in a decrease of Re accumulation in the dendritic core. The influence of Ru on the microstructural stability toward the topologically close-packed phase formation was discussed based on Scheil type calculations with experimentally determined microsegregation results.

Effect of Addition of β-TCP on Bioactivity and Mechanical Properties of Biodegradable PLA/β-TCP Composites (β-TCP 첨가량에 따른 생분해성 PLA/β-TCP 복합체의 생체활성과 기계적 특성 평가)

  • Moon, Hee-Jung;Kim, Seol-Ak;Kim, Dong-Ae
    • The Journal of the Korea Contents Association
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    • v.21 no.3
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    • pp.609-615
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    • 2021
  • The aim of the present study was to investigate the mechanical properties of melt-injected poly lactic acid (PLA) composites with β-tricalcium phosphate (β-TCP). The PLA mixed with calcined PLA/β-TCP powder to be contents of 0, 10, 30, 50 wt%, respectively, was dissolved in chloroform solvent under stirring for 24 h. Then the liquid mixtures were dropped into ethanol to extract solvent. After drying, the well-dispersed PLA/β-TCP composites were granulated and melt-injected to prepare specimens for various mechanical testing. PLA/β-TCP induced the precipitation of an apatite bone-mineral phase on the surface after immersion in a human simulated body fluid (SBF) for 90 days, showing in bioactivity. Mean various mechanical properties PLA/β-TCP composite were increased up to 10-30 wt% with significantly in part and composite were decreased 50 wt% of showing in mechanical properties. In conclusion, Over 30 wt% addition of β-TCP to PLA may be not advisable to improve the mechanical properties of melt-injected polymeric composites. Results indicated that β-TCP can be used considered as potential reinforcing agent for increasing mechanical properties for PLA. Therefore, it was suggest that the additional effects of β-TCP and research on a wide range of substances.

Antimicrobial effect of Enterococcus faecalis BMSE-HMP005 isolated from human breast milk against multidrug-resistant bacteria (모유 유래 유산균 Enterococcus faecalis BMSE-HMP005의 다제내성 균에 대한 항균효과)

  • Lee, Jeong-Eun;Kim, Soo-bin;Yu, Du-na;Jo, So-Yeon;Kim, Ae-Jung;Kook, Moochang
    • Korean Journal of Food Science and Technology
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    • v.54 no.2
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    • pp.209-217
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    • 2022
  • In this study, Enterococcus faecalis BMSE-HMP005 isolated from human breast milk demonstrated antimicrobial effects against multidrug-resistant (MDR) bacterial strains. The bacteriocin produced by E. faecalis BMSE-HMP005 was fractionated using reverse-phase high-performance liquid chromatography. This fraction showed antimicrobial effects against both gram-positive and gram-negative MDR bacteria. No hemolytic reactions were observed. E. faecalis BMSEHMP005 was resistant to vancomycin; however, kanamycin, ampicillin, and erythromycin showed minimum inhibitory concentrations that were lower than the acceptable range provided by the European Food Safety Authority. For artificial gastric juice and bile acid, the survival rates were 98.67% and 95.70%, respectively. These results show the potential utility of E. faecalis BMSE-HMP005 as a probiotic with remarkable antimicrobial effects against MDR bacteria.

Comparative Study on the Growth Condition of Landscape Woody Plants according to the Ground Structure - Focusing on Manseok Beach Town Complex 2, Incheon - (지반구조에 따른 수목 생육상태 비교 연구 - 인천광역시 만석비치타운 단지를 대상으로 -)

  • Cho, Sung-Ho;Han, Bong-Ho;Park, Seok-Cheol
    • Journal of the Korean Society of Environmental Restoration Technology
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    • v.25 no.3
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    • pp.63-82
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    • 2022
  • The purpose of this study is to compare growth condition of landscape woody plants growing on the different ground structures in apartment complex. I chose Manseok Beach Town Complex 2, in Manseok-dong, Seo-gu, Incheon which has both natural and artificial ground as a subject site. Analysis of three phases of soil showed that artificial ground had average liquid phase of 30.89%, artificial ground mounding 33.88% and natural ground 24.40%. It means that artificial ground has higher water content than natural ground despite having same earthiness. It is believed that artificial ground is not as well drained as natural ground even though it is connected to the natural ground and has a deep soil depth because of mounding. Comparative study between woody plants on natural ground and those on artificial ground demonstrated that trees on natural ground grew 40.4% compared to those on artificial ground(0.875mm more) in terms of diameter growth. Average diameter growth of trees on natural ground was 3.040mm against 2.165mm for those on artificial ground. All 19 tree species which were measured for root diameter growth showed similar or higher growth on natural ground than on artificial ground. When it comes to growth of height, arborvitae showed highest growth on natural ground, followed by Thuja occidentalis, Pinus strobus, Magnolia denudata, Diospyros kaki and Aesculus turbinata. I measured branch growth and rate of leaf adherence of Pinus strobus. Average annual rate of branch growth of woody plants on natural ground was twice as high as those on artificial ground. I could conclude that ground structure influences branch growth of Pinus strobus. Statistics analysis of tree damage demonstrated significant result, meaning that there is a difference in the average damage rate depending on structure of ground. In order to validate growth difference by planting ground, I conducted T-Test of growth of diameter, root diameter, branch and height on woody plants growing on natural and artificial ground. As a result, it is believed that there is a difference in the growth of trees depending on the ground structure. Putting all these results together demonstrates that woody plants on natural ground generally grow better than those on artificial ground, which means ground structure does have an influence on the environment of growth of trees.

Improvement of Reproductive Efficiency of Artificial Insemination Following Estrus Induction in Dog II. Semen Freezing and Artificial Insemination in Dog

  • Lee, Y. R.;S. Y. Choe
    • Proceedings of the Korean Society of Embryo Transfer Conference
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    • 2002.11a
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    • pp.92-92
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    • 2002
  • Considerable attention has been focused on the cryopreservation of semen and estrus induction in dog, as consequence of poor productivity caused by long anestrus period, in order to enhance the productivity of youngs and to preserve the breeds. The objectives of this study were to evaluate semen quality after cryopreservation and to evaluate the Pregnancy rate after insemination (AI). Fifty infertilie dogs (age 2∼3 years) were selected for the study and divided into three different estrus induction treatment groups. Group 1: dogs (n=15) were given clomifene (0.1 mg/kg) orally for five days at 12 hr intervals. Group 2: dogs (n=15) were given bromocriptine (50 $\mu\textrm{g}$/kg) orally for five days at 12 hr intervals, followed by single injection intravenously of 500 IU GnRH (Group 3, n=20) when pro-estrus occurred. The rates of pregnancy in estrus inducted dogs mated naturally compared to those inseminated artificially with ejaculated fresh semen and frozen-thawed semen. Estrus detection was performed using the method of vaginal smear and confirmed by the plasma progesterone assay. The ejaculated semen to freeze was exposed to a mixture of Tris extender with cryoprotectant (Trisma, 81 mM: TES, 209 mM: citric acid, 6 mM; glucose, 5 mM; glycerol, 8%) and cryopreserved gradually by slow-cooling at 17 cm above the surface of liquid nitrogen (LN$_2$) for 23 min. The motility of frozen-thawed spermatozoa was assessed by phase-contrast microscopy. To assess their viability and acrosome content, spermatozoa were stained with a vital stain and Fluorescence conjugated lectin Pisum Savitum Agglutinin (FITC/PAS), respectively. Pregnancy was confirmed by ultrasonograpy on day 25, 35 and 55 post insemination. The use of fresh semen, the pregnancy rates were observed 66.6, 66.6, 75.0 and 83.3% in natural estrus, clomifene induced, bromocriptine induced and a combination of GnRH and bromocriptine, respectively. The use of frozen-thawed semen, the pregnancy rates were observed 66.6, 33.3, 50.0 and 60.0% in natural estrus, clomifene induced, bromocriptine induced and a combination of GnRH and bromocriptine, respectively. No difference was observed in the number of offspring produced among natural estrus and treated groups inseminated with fresh or frozen-thawed semen. In conclusion, the pregnancy rate of dogs treated with a combination of GnRH and bromocriptine was more effective than use of clomifene or bromocriptine only. In addition, frozen-thawed semen can be used successfully far artificial insemination in dog.

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