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http://dx.doi.org/10.7783/KJMCS.2019.27.2.96

Variation in Fatty Acid Composition, Caffeic and Rosmarinic Acid Content, and Antioxidant Activity of Perilla Accessions  

Kim, Da Jeong (National Agrobiodiversity Center, NIAS, RDA)
Assefa, Awraris Derbie (National Agrobiodiversity Center, NIAS, RDA)
Jeong, Yi Jin (National Agrobiodiversity Center, NIAS, RDA)
Jeon, Young Ah (International Technology Cooperation Center, RDA)
Lee, Jae Eun (National Agrobiodiversity Center, NIAS, RDA)
Lee, Myeong Chul (National Agrobiodiversity Center, NIAS, RDA)
Lee, Ho Sun (National Agrobiodiversity Center, NIAS, RDA)
Rhee, Ju Hee (National Agrobiodiversity Center, NIAS, RDA)
Sung, Jung Sook (National Agrobiodiversity Center, NIAS, RDA)
Publication Information
Korean Journal of Medicinal Crop Science / v.27, no.2, 2019 , pp. 96-107 More about this Journal
Abstract
Background: The aim of this study was to compare the fatty acid composition, antioxidant activity, and the content of two major phenolic acids, caffeic and rosmarinic acids, of 45 Perilla accessions collected from Russia. Methods and Results: A total of 45 accessions of the genus Perilla were used in this study. The antioxidant activities of these accessions were analyzed using a spectrophotometer, and their caffeic and rosmarinic acid contents were determined using a reversed-phase ultraperformance liquid chromatography (UPLC) system. The Perilla seed oil was recovered using hexane in a soxhlet extraction method. The fatty acid compositions were analyzed using a Shimadzu QP2010 gas chromatography-mass spectoroscopy system. The results showed that accessions IT226732 and IT274300 had the highest content of caffeic and rosmarinic acid, respectively. A cluster analysis based on antioxidant assay results and concentration of phenolic acids led to the categorization of Perilla accessions into three major groups. The accessions in Group I were characterized by higher caffeic and rosmarinic acid content and antioxidant activity, compared to the accessions in the other two groups (p < 0.05). The total oil content ranged between 28.39 and 46.89%. The compositions of oleic, linoleic, and linolenic acid ranged from 11.83 to 19.55%, 11.92 to 16.71%, and 59.19 to 67.28%, respectively. Conclusions: The results of this study indicated that accessions IT274300, IT226732, IT274293, IT235818, and IT235820 could be used as a source of functional materials.
Keywords
Perilla; Plant Germplasm; Functional Component; Oil Composition;
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Times Cited By KSCI : 10  (Citation Analysis)
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1 Hyun KW, Kim JH, Song KJ, Lee JB, Jang JH, Kim YS and Lee JS. (2003). Physiological functionality in geumsan perilla leaves from greenhouse and field cultivation. Korean Journal of Food Science and Technology. 35:975-979.
2 Jennings PE and Barnett AH. (1988). New approaches to the pathogenesis and treatment of diabetic microangiopathy. Diabetic Medicine. 5:111-117.   DOI
3 Jeong HS, Jeong JT, Lee JH, Park CG, Choi JH, Jang GY, Kim JW, Chang JK, Kim DH and Lee SE. (2018). Comparison of anti-oxidative and anti-inflammatory effect of Atractylodes interspecific hybrid cultivar roots. Korean Journal of Medicinal Crop Science. 26:391-400.   DOI
4 Jo JH, Bae EY, Lee TK, Kim MH, Lee SW, Kim BS and Lim CH. (2016). Antioxidative activities of Sanguisorba officinalis L. in diabetic rats. Korean Journal of Medicinal Crop Science. 24:152-158.   DOI
5 Lee AY, Choi JM, Lee MH, Lee JM, Lee S and Cho EJ. (2018). Protective effects of perilla oil and alpha linolenic acid on SH-SY5Y neuronal cell death induced by hydrogen peroxide. Nutrition Research and Practice. 12:93-100.   DOI
6 Lee HS, Lee HA, Hong CO, Yang SY, Hong SY, Park SY, Lee HJ and Lee KW. (2009). Quantification of caffeic acid and rosmarinic acid and antioxidant activities of hot-water extracts from leaves of Perilla frutescens. Korean Journal of Food Science and Technology. 41:302-306.
7 Oh SI and Lee MS. (2003). Screening for antioxidative and antimutagenic capacities in 7 common vegetables taken by Korean. Journal of the Korean Society of Food Science and Nutrition. 32:1344-1350.   DOI
8 Park JH, Lee CO, Yoo JH, An NTL, Yoo NH and Kim MJ. (2018). Antioxidative and inhibitory activities of extract and juice powder from Hippophae rhamnoides L. against nitric oxide and elastase production. Korean Journal of Medicinal Crop Science. 26:119-126.   DOI
9 Re R, Pellegrini N, Proteggente A, Pannala A, Yang M and Rice-Evans C. (1999). Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Biology and Medicine. 26:1231-1237.   DOI
10 Piao XM, Jang EK, Chung JW, Lee GA, Lee HS, Sung JS, Jeon YA, Lee JR, Kim YG and Lee SY. (2013). Variation in antioxidant activity and polyphenol content in tomato stems and leaves. Plant Breeding and Biotechnology. 1:366-373.   DOI
11 Ryu HS and Kim HS. (2008). Studies on the effects of water extract from mixture of pine needles, Sedum sarmentosum bunge, hijkiaorme, buckwheat, and perilla leaves on the immune function activation. Korean Journal of Food and Nutrition. 21:269-274.
12 Seong ES, Kim SK, Lee JW, Choi SH, Yoo JH, Lim JD, Na JK and Yu CY. (2018). Antioxidant and antibacterial activities of the byproducts of Abies holophylla extract. Korean Journal of Medicinal Crop Science. 26:134-140.   DOI
13 Shekarchi M, Hajimehdipoor H, Saeidnia S, Gohari AR and Hamedani MP. (2012). Comparative study of rosmarinic acid content in some plants of Labiatae family. Pharmacognosy Magazine. 8:37-41.   DOI
14 Sung JS, Jeong YJ, Kim DJ, Assefa AD, Jeon YA, Hur OS, Ro NY, Ko HC, Ok HC, Rhee JH, Lee MC and Baek HJ. (2018). Oil compositions and antioxidant properties of safflower germplasm collected from east asia. Korean Journal of Medicinal Crop Science. 26:32-41.   DOI
15 Um JN, Min JW, Joo KS and Kang HC. (2017). Antioxidant, anti-wrinkle activity and whitening effect of fermented mixture extracts of Angelica gigas, Paeonia lactiflora, Rehmannia chinensis and Cnidium officinale. Korean Journal of Medicinal Crop Science. 25:152-159.   DOI
16 Villalva M, Jaime L, Aguado E, Nieto JA, Reglero G and Santoyo S. (2018). Anti-inflammatory and antioxidant activities from the basolateral fraction of caco-2 cells exposed to a rosmarinic acid enriched extract. Journal of Agricultural and Food Chemistry. 66:1167-1174.   DOI
17 Asif M. (2012). Phytochemical study of polyphenols in Perilla Frutescens as an antioxidant. Avicenna Journal of Phytomedicine. 2:169-178.
18 Yamazaki M and Saito K. (2006). Isolation and characterization of anthocyanin 5-O-glucosyltransferase in Perilla frutescens var. Crispa by differential display. Differential Display Methods and Protocols. 317:255-266.   DOI
19 Yen GC and Chen HY. (1995). Antioxidant activity of various tea extracts in relation to their antimutagenicity. Journal of Agricultural and Food Chemistry. 43:27-32.   DOI
20 Waterhouse AL. (2002). Determination of total phenolics. In current protocols in food analytical chemistry. I1.1.1-I1.1.7. John Wiley and Sons. New York. NY, USA. p.1-4.
21 Fridovich I. (1989). Superoxide dismutase an adaption to paramagnetic gas. Journal of Biological Chemistry. 264:7761-7764.   DOI
22 Benzie IFF and Strain JJ. (1996). The ferric reducing ability of plasma (FRAP) as a measure of "antioxidant power": The FRAP assay. Analytical Biochemistry. 239:70-76.   DOI
23 Cho MS. (2003). A study of intakes of vegetables in Korea. Korean Journal Food Culture. 18:601-612.
24 Choi HG, Tran PT, Lee JH, Min BS and Kim JA. (2018). Antiinflammatory activity of caffeic acid derivatives isolated from the roots of Salvia miltiorrhiza Bunge. Archives of Pharmacal Research. 41:64-70.   DOI
25 Ganguly R, Hasanally D, Stamenkovic A, Maddaford TG, Chaudhary R, Pierce GN and Ravandi A. (2018). Alpha linolenic acid decreases apoptosis and oxidized phospholipids in cardiomyocytes during ischemia/reperfusion. Molecular and Cellular Biochemistry. 437:163-175.   DOI
26 Han HM, Kwon YS and Kim MJ. (2016). Antioxidant and antiproliferative activity of extracts from water chestnut(Trapa japonica Flerow). Korean Journal of Medicinal Crop Science. 24:14-20.   DOI
27 Hong YP, Kim SY and Choi WY. (1986). Postharvest changes in quality and biochemical components of perilla leaves. Korean Journal of Food Science and Technology. 18:255-258.