• Journal of the Korean Wood Science and Technology
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• v.15 no.3
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• pp.14-23
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• 1987

This study was carried out to investigate the anatomical characteristics, the radial and axial variation of the cell dimension and bulk density for four bamboo species used as the major material of bamboo products in Korea; Phyllostachys(P.bambusoides, P.edulis, P.nigra var.henonis, and Pnigra). The results obtained were summarized as follows; 1) In the outer part of culm, the mophological difference of cell diameter and distribution were not acknowledged. However, gum-like substance was occurred In the epidermis, hypodermis, cortex and metaxylem vessel of P.nigra, while it was occured only in the metaxylem vessel of P.edulis. 2) The protoxylem vessel in the central zone of culm-wall could be in the two types; the first type consisted of 4-5 small cell pieces occurred in P.edulis and P.nigra var. henonis, the second type of 1-2 large cell pieces in P.bambusoides and P.nigra. And transition of cell diameter from the vascular bundle sheath to the ground tissue was gradually enlarged in P.nigra and P.edulis but abruptly in P.bambusoides and P.nigra var. henonis. 3) Thin-wall tyloses were well developed in the outer and central zone of culm-wall in four species. 4) Compared with the central part of culm-wall, the number of parenchyma cells and the amount of the vascular bundle sheaths per unit area were a few in the inner part. The inner part nearest to the pith cavity was consisted of thick-well parenchyma cell. 5) The dimensional variations of metaxylem vessel and parenchyma cell in the radial direction were decreased from the inner part to the epidermis in all species observed. 6) The fiber length was the maximum in the central zone and its diameter was increased from the inner part to the outer part. In the axial variation. fiber length was slightly increased from the base and then decresed slowly toward the top, and its diameter was reverse. 7) The axial variation of the bulk density was continuously increased from the base toward the top and its radial variation was rapidly increased from the inner part to the epidermis.

• The Journal of Pediatrics of Korean Medicine
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• v.28 no.2
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• pp.72-87
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• 2014

Objectives This study was carried out to investigate the effect of Ganoderma lucidum extract (GLE) and microneedle therapy system (MTS) on hair growth in an alopecia model of C57BL/6N mice. Methods Five-week old mice were depilated and separated in 4 groups ; CON (50% EtOH), MXD (5% Minoxidil), MTS and GLE + MTS. The treatments were applied twice a week for 3 weeks. The hair growth was determined photographically, the hair density, thickness and length were identified by Folliscope and the weights of body and organs were measured. In dorsal skin tissue, the expression of hair growth-related gene and protein was analyzed by RT - PCR or Western blot. In addition, the hair follicles in the dermis were observed by H&E staining. Results The promotion of hair growth was observed in GLE + MTS and MTS compared to CON. The hair density, thickness and length were also improved in GLE + MTS and MTS compared to CON. The mRNA expression of TGF-${\alpha}$, TGF-${\beta}1$, IGF-1, PRL and PL and the protein expression of VEGF and IGF-1 were increased in GLE + MTS and MTS compared to CON. The hair follicles and hair root growth were improved in GLE + MTS and MTS compared to CON. In the above results, GLE + MTS were more effective than MTS. Conclusions These results suggest that GLE and MTS has a hair growth activity and can be useful for the treatment of alopecia.

• Food Science of Animal Resources
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• v.36 no.6
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• pp.819-828
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• 2016

Muscle fiber characteristics account for meat quality and muscle fibers are mainly classified into three or more types according to their contractile and metabolic properties. However, the majority of previous studies on bovine skeletal muscle are based on myosin ATPase activity. In the present study, the differences in the characteristics of muscle fibers classified by the expression of myosin heavy chain (MHC) among four bovine skeletal muscles such as longissimus thoracis (LT), psoas major (PM), semimembranosus (SM) and semi-tendinosus (ST) and their relationships to beef quality were investigated. MHCs 2x, 2a and slow were identified by LC-MS/MS and IIX, IIA and I fiber types were classified. PM, which had the smallest size and highest density of fibers regardless of type, showed the highest myoglobin content, CIE $L^*$, $a^*$, $b^*$ and sarcomere length (p<0.05), whereas ST with the highest composition of IIX, showed high shear force and low sarcomere length (p<0.05). The correlation coefficients between muscle fiber characteristics and meat quality showed that type IIX is closely related to poor beef quality and that a high density of small-sized fibers is related to redness and tenderness. Therefore, the differences in meat quality between muscles can be explained by the differences in muscle fiber characteristics, and especially, the muscles with good quality are composed of more small-sized fibers regardless of fiber type.

• Journal of Biomedical Engineering Research
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• v.43 no.1
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• pp.11-18
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• 2022

An electromagnetic generator with variable stimulation parameters is required to conduct basic research on magnetic flux density and frequency for pulsed electromagnetic fields (PEMFs). In this study, we design an electromagnetic generator that can conduct basic research by providing parameters optimized for cell and animal experimental conditions through adjustable stimulation parameters. The magnetic core was selected as a solenoid capable of uniform and stable electromagnetic stimulation. The solenoid was designed in consideration of the experimental mouse and cell culture dish insertion. A voltage and current adjustable power supply for variable magnetic flux density was designed. The system was designed to be adjustable in frequency and pulse width and to enable 3-channel output. The reliability of the system and solenoid was evaluated through magnetic flux density, frequency, and pulse width measurements. The measured magnetic flux density was expressed as an image and qualitatively observed. Based on the acquired image, the stimulation area according to the magnetic flux density decrease rate was extracted. The PEMF frequency and pulse width error rates were presented as mean ± SD, and were confirmed to be 0.0928 ± 0.0934% and 0.529 ± 0.527%, respectively. The magnetic flux density decreased as the distance from the center of the solenoid increased, and decreased sharply from 60 mm or more. The length of the magnetic stimulation area according to the degree of magnetic flux density decrease was obtained through the magnetic flux density image. A PEMF generator and stimulation parameter control system suitable for cell and animal models were designed, and system reliability was evaluated.

• Proceedings of the Korean Geotechical Society Conference
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• 1999.10a
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• pp.3-44
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• 1999

This paper discusses the lateral behavior of single and group piles in homogeneous and non-homogeneous(two layered) soil. In the single pile, the model tests were conducted to investigate the effects on ratio of lower layer height to embedded pile length, ratio of soil modules of upper layer to lower layer, boundary rendition of pile head and tip, embedded pile length, pile construction condition, ground condition with saturate and moisture state in Nak-Dong river sand. Also, in the group pile, the model tests were to investigate the effects on spacing-to-diameter ratio of pile, pile array, ratio of pile spacing, boundary condition of pile head and tip, eccentric load and ground condition. The maximum bending moment and deflection induced in active piles were found to be highly dependent on the relative density, pile construction condition, boundary condition of pile head and tip. Based on the results obtained, it was found that the decrease of lateral bearing capacity in saturated sand was in the range of 31% - 53% as compared with the case of dry sand. Also, in the group pile, a spacing-to-diameter of 6.0 seems to be large enough to eliminate the group effect for the case of relative density of 61.8%, and 32.8%, and then each pile in such a case behaves essentially the same as a single pile. In this study, the program is developed by using the modified Chang method which used p - y method and the exact solution of governing equation of pile and it can be used to calculate the deflection, bending moment and soil reaction with FDM in non-homogeneous soil. In comparing the modified Chang method with field test results, the predict results shows better agreement with measured results in field tests.

• The Journal of Korean Medicine
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• v.41 no.4
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• pp.27-40
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• 2020

Objectives: This study was conducted to evaluate the hair growth-promoting effects by Rumex japonicas Houttuyn ethanol extract (RJHEE) in C57BL/6N mice and HaCaT cells. Methods: The hair growth effect was examined by topical application of RJHEE on the shaved dorsal skin of C57BL/6 mice. Six-week old mice were depilated and separated in 4 groups; CON (vehicle treatment), MXD (2% Minoxidil), and RJHEE (2% and 4%). The treatments were applied daily for 17 days. The hair growth was determined photographically and the hair density, thickness and length were identified by Folliscope. In dorsal skin tissue, the expression of hair growth-related protein was analyzed by Western blotting. In HaCaT cells, the cell proliferation and the protection against H₂O₂-induced cell damage by RJHEE were analyzed. Results: Our results indicate that RJHEE promote the hair growth, hair density, thickness and length. RHE activate the Wnt/𝛽-catenin signaling and induced the expression of cell survival-related proteins, such as pERK/ERK and Bcl-2/Bax. In HaCaT, RJHEE accelerated the cell proliferation and protected the H₂O₂-induced cell damage. Conclusions: Our results strongly suggest that RJHEE promotes hair growth by regulating the activation of Wnt/𝛽-catenin signaling and cell survival signaling and protects oxidative stress-induced hair damage. Therefore, RJHEE has a hair growth activity and can be useful for the treatment of alopecia.

• Bulletin of the Korean Chemical Society
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• v.33 no.10
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• pp.3285-3292
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• 2012

The interaction of thiazole drug with (6,0) zigzag single-walled boron nitride nanotube of finite length in gas and solvent phases was studied by means of density functional theory (DFT) calculations. In both phases, the binding energy is negative and presenting characterizes an exothermic process. Also, the binding energy in solvent phase is more than that the gas phase. Binding energy corresponding to adsorption of thiazole on the BNNT model in the gas and solvent phases was calculated to be -0.34 and -0.56 eV, and about 0.04 and 0.06 electrons is transferred from the thiazole to the nanotube in the phases. The significantly changes in binding energies and energy gap values by the thiazole adsorption, shows the high sensitivity of the electronic properties of BNNT towards the adsorption of the thiazole molecule. Frontier molecular orbital theory (FMO) and structural analyses show that the low energy level of LUMO, electron density, and length of the surrounding bonds of adsorbing atoms help to the thiazole adsorption on the nanotube. Decrease in global hardness, energy gap and ionization potential is due to the adsorption of the thiazole, and consequently, in the both phases, stability of the thiazole-attached (6,0) BNNT model is decreased and its reactivity increased. Presence of polar solvent increases the electron donor of the thiazole and the electrophilicity of the complex. This study may provide new insight to the development of functionalized boron nitride nanotubes as drug delivery systems for virtual applications.

• Korean Journal of Acupuncture
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• v.34 no.3
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• pp.146-155
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• 2017

Objectives : This study was carried out to investigate the effects of Crataegi Fructus water extract(CFWE) on hair growth in an alopecia model of C57BL/6N mice and human dermal papilla cells(hDPCs). Methods : Six-week old mice were depilated and separated in 3 groups ; CON, MXD(2% Minoxidil), and CFWE. The treatments were applied twice a day for 18 days. The hair growth was determined photographically. The hair density, thickness and length were identified by Folliscope and the weights of body were measured. In dorsal skin tissue, the expression of hair growth-related protein was analyzed by Western blot. In hDPCs with/without $IFN-{\gamma}$, cell proliferation and the expression of hair growth-related genes were analyzed. Results : We observed that CFWE promoted hair growth compared to CON. CFWE improved the hair density, thickness and length compared to CON. CFWE increased the $Wnt/{\beta}$-catenin signaling in dorsal skin. In hDPCs, CFWE accelerated the cell proliferation and inhibited $IFN-{\gamma}$-induced hDPCs degeneration. CFWE increased the mRNA expression of ${\beta}$-catenin, Axin-2, BMP-4, FGF-7, FGF-10, and ALP compared to CON and $IFN-{\gamma}$ treated cells. Conclusions : These results suggest that CFWE has a hair regrowth activity via $Wnt/{\beta}$-catenin signaling and can be useful for the treatment of alopecia.

• Journal of Sensor Science and Technology
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• v.18 no.1
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• pp.86-94
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• 2009

Temperature measurement capability was inter-compared using the transfer standard platinum resistance thermometers(SPRT) among four laboratories of KRISS. The transfer SPRTs were primarily calibrated at the triple point of water and Ga melting point, then used at inter-comparison experiment. Temperature difference of calibration value between temperature laboratory and length laboratory at $20^{\circ}C$ was -0.7 mK and +2.4 mK at density laboratory. Temperature measured near $20^{\circ}C$, $25^{\circ}C$ and $30^{\circ}C$ at fluid flow laboratory was deviated by $34.2{\sim}80.4\;mK$ from the calibration values of the transfer SPRT. Ga melting points was inter-compared among three laboratories, and the difference of Ga melting points against the standard Ga melting point of temperature laboratory were $0.03{\sim}0.54\;mK$ at length laboratory and 0.02 mK at density laboratory.

• Journal of Korea Technical Association of The Pulp and Paper Industry
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• v.34 no.4
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• pp.51-60
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• 2002

This study was carried out to develop the biochemical pulping method to enhance energy saving and decrease the capital cost through the soda pulping under atmospheric pressure (100%). Nonwood substrates, rice straw, were pretreated by white-rot fungi, Ganoderma Iucidum. It has acquired several basic data that can be applied in bio-soda pulping. The results of this study were as follow. Without any nutrients or with glucose, N and glucose+N the weight losses of rice straws inoculated by Canoderma Iucidum were 8.5~29.8%, 9.3~32% 11.8~30.1% and 11.8~24.4% respectively for 5, 10, 15, 20, 25 and 30 days incubation. The more extending incubation periods, the more increasing weight losses. The yield of untreated rice saw was 54.8% after pulping. When any nutrients was not added or glucose, N and glucose+N were added for the pretreatment, the total yields were ranged 50.6~38.5%, 48.6~34.4%, 47.2~38.4% and 49.5~42.6% respectively for 5, 10, 15, 20, 25 and 30 days incubation. The yields were gradually decreased based on extending of incubation. The physical properties of rice straw soda pulps without fungal treatment, the density, breaking length, burst index, tear index and folding endurance were 0.24 g/$\textrm{cm}^3$, 2.32 km, 0.91 kPa $m^2$/g, 56.7 mN.$m^2$/g and 35 times, respectively. After pretreatment without any nutrients or with glucose, N and glucose+N as nutrients the density was 0.27~0.30 g/$\textrm{cm}^3$, the breaking length 3.14~5.25 km, burst index 1.42~2.78 kPa.$\textrm{m}^2$/g, tear index45.8~64.5 mN.$\textrm{m}^2$/g and folding endurance 47~288 times at all incubating periods when pulping was done. The physical properties were increased with the increasing incubation periods. However, when glucose+N was added, the physical properties were shown superior results each incubating duration.